##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138797_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8491924
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3211199252372	33.0	31.0	34.0	31.0	34.0
2	32.50345916897042	34.0	31.0	34.0	31.0	34.0
3	32.62722817585273	34.0	31.0	34.0	31.0	34.0
4	36.15131258828977	37.0	35.0	37.0	35.0	37.0
5	35.97676203885009	37.0	35.0	37.0	35.0	37.0
6	36.016677610397835	37.0	35.0	37.0	35.0	37.0
7	35.99750633660876	37.0	35.0	37.0	35.0	37.0
8	35.98292872145347	37.0	35.0	37.0	35.0	37.0
9	37.82868935237762	39.0	38.0	39.0	35.0	39.0
10-11	37.707662598016654	39.0	37.0	39.0	35.0	39.0
12-13	37.73082254386638	39.0	37.0	39.0	35.0	39.0
14-15	39.05903556131685	40.0	38.0	41.0	36.0	41.0
16-17	39.012325946393304	40.0	38.0	41.0	36.0	41.0
18-19	39.068759388331785	40.0	38.5	41.0	36.0	41.0
20-21	39.06619584021469	40.0	39.0	41.0	36.0	41.0
22-23	39.03305436311008	40.0	39.0	41.0	36.0	41.0
24-25	39.02224066215467	40.0	39.0	41.0	36.0	41.0
26-27	38.93124027369208	40.0	38.0	41.0	35.0	41.0
28-29	38.84895339264591	40.0	38.0	41.0	35.0	41.0
30-31	38.71329231778039	40.0	38.0	41.0	35.0	41.0
32-33	38.630305161139404	40.0	38.0	41.0	35.0	41.0
34-35	38.59208191006168	40.0	38.0	41.0	35.0	41.0
36-37	38.49417855080017	40.0	38.0	41.0	34.0	41.0
38-39	38.46016292635568	40.0	38.0	41.0	34.0	41.0
40-41	38.45994461174959	40.0	38.0	41.0	34.0	41.0
42-43	38.42266053410644	40.0	38.0	41.0	34.0	41.0
44-45	38.316211222799026	40.0	38.0	41.0	34.0	41.0
46-47	38.26244591452662	40.0	38.0	41.0	34.0	41.0
48-49	38.23927239063735	40.0	38.0	41.0	34.0	41.0
50-51	38.16232859256354	40.0	37.5	41.0	33.0	41.0
52-53	38.027143152572016	40.0	37.0	41.0	33.0	41.0
54-55	37.85128015825351	40.0	37.0	41.0	33.0	41.0
56-57	37.72693283806497	39.0	37.0	41.0	33.0	41.0
58-59	37.50404859420265	39.0	36.0	41.0	32.5	41.0
60-61	37.337590250615555	39.0	36.0	40.0	32.0	41.0
62-63	37.12125137138593	39.0	35.0	40.0	32.0	41.0
64-65	36.793975819084565	38.0	35.0	40.0	31.0	41.0
66-67	36.718831633443045	38.0	35.0	40.0	31.0	41.0
68-69	36.48114361162032	37.5	35.0	40.0	31.0	41.0
70-71	36.15926327282363	37.0	35.0	39.5	31.0	41.0
72-73	35.840200747728915	36.5	34.5	39.0	31.0	41.0
74-75	35.432874992499805	36.0	34.0	39.0	30.5	40.0
76-77	33.390814488333405	34.5	32.0	36.5	27.5	39.0
78-79	34.56025357705823	35.0	34.0	37.0	30.0	39.0
80-81	34.5678913698673	35.0	34.0	37.0	30.0	39.0
82-83	34.284864584074285	35.0	34.0	36.0	30.0	38.5
84-85	34.03265227729922	35.0	34.0	36.0	30.0	37.0
86-87	33.829115808824355	35.0	34.0	35.5	30.0	37.0
88-89	33.59906946473522	35.0	33.0	35.0	29.5	36.5
90-91	33.439873700438596	35.0	33.0	35.0	29.0	36.0
92-93	33.37915040131418	35.0	33.0	35.0	29.0	36.0
94-95	33.36124206809844	35.0	33.0	35.0	29.5	36.0
96-97	33.39963550323321	35.0	33.0	35.0	30.0	35.5
98-99	33.43155779595307	35.0	33.0	35.0	30.0	35.0
100-101	32.195137372031866	34.0	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	7.0
20	32.0
21	155.0
22	691.0
23	2312.0
24	6146.0
25	13769.0
26	25696.0
27	43094.0
28	64414.0
29	89782.0
30	120218.0
31	158752.0
32	207912.0
33	283723.0
34	407732.0
35	628131.0
36	1108547.0
37	2031200.0
38	2371754.0
39	916935.0
40	10920.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43318993434232	20.434379770709203	14.08690186110945	24.04552843383902
2	14.583099031754983	21.145341091478127	41.96342740293908	22.3081324738278
3	18.487803057093195	25.88825876070708	30.215417638619662	25.40852054358006
4	12.548007388668903	17.9678522718414	39.02644183495798	30.457698504531717
5	13.273435582735393	37.80275264145335	35.7534742453882	13.170337530423051
6	30.02816558414795	38.12728422910992	18.831280166897397	13.013270019844736
7	26.594514976817972	32.40999330658164	23.303164277023676	17.692327439576708
8	23.907712812340915	37.70962223572793	21.212174025478213	17.170490926452946
9	25.355841492959243	17.110985475239797	21.318258912921035	36.214914118879925
10-11	24.15187633612273	26.922226793887177	29.109175755500345	19.816721114489745
12-13	24.869319571511625	24.28877215802967	29.256135759504442	21.585772510954264
14-15	21.94344426206854	25.82168810164152	26.64297435086409	25.591893285425854
16-17	21.763631083724945	28.98333560609326	27.288987194587584	21.96404611559422
18-19	21.849512548628557	28.308137237215032	28.71246257031975	21.12988764383666
20-21	22.555849445687596	27.489945560027728	28.556470173363905	21.39773482092077
22-23	22.255874421479124	27.40646871651763	28.429158482700835	21.908498379302415
24-25	22.05376509445359	27.52408230275058	28.4889528946265	21.933199708169333
26-27	22.047992341249017	27.693033414294334	28.39288195268925	21.866092291767398
28-29	21.99188411949477	27.789842030709455	28.40245687425102	21.815816975544756
30-31	22.3049319294281	27.58961102884622	28.2964684408879	21.808988600837775
32-33	22.030846012349347	27.777374482981664	28.27625343529205	21.91552606937694
34-35	22.13466938897451	27.79033880183517	28.27497804791704	21.80001376127328
36-37	22.172118380806502	27.747472339303275	28.2388026613895	21.841606618500727
38-39	21.99249287482557	27.832915682507714	28.337044938298227	21.837546504368486
40-41	22.229652883248814	27.680879908897293	28.190651295551657	21.89881591230224
42-43	22.145218585578004	27.756221123253923	28.081531563854483	22.017028727313594
44-45	22.1856758853084	27.828404498829435	27.969541896138217	22.01637771972395
46-47	22.24725177058013	27.780123408782565	27.75158027173316	22.221044548904135
48-49	22.162818035988966	27.88215759534458	27.7975098394256	22.157514529240853
50-51	22.171408368011473	27.954516209683288	27.73193047541927	22.142144946885967
52-53	22.328999846177354	27.870499367059743	27.62099491045435	22.179505876308557
54-55	22.29189572291665	27.788829429032084	27.64125340490632	22.27802144314495
56-57	22.281424285372452	27.914445849756643	27.626031085739527	22.17809877913138
58-59	22.285521022043156	27.939187871229237	27.596762926551634	22.17852818017597
60-61	22.361051797865475	27.916426688783602	27.592170992002053	22.13035052134887
62-63	22.322616869497026	27.94430211330838	27.556266222772802	22.17681479442179
64-65	22.298383997540448	27.901406556243035	27.55982805096982	22.240381395246697
66-67	22.347244500263496	27.93714027719813	27.421999945315957	22.29361527722242
68-69	22.29356354654216	27.913723687840246	27.515136247466	22.277576518151594
70-71	22.285900857963348	27.733266446133126	27.57255952611461	22.408273169788917
72-73	22.354619496450212	27.752091222387353	27.496583646594917	22.396705634567514
74-75	22.21701436904467	27.854382510262642	27.51399738818689	22.4146057325058
76-77	22.32108407743423	27.851010220129545	27.438485790951955	22.389419911484268
78-79	22.317302917308517	27.88829302195198	27.390516401351633	22.40388765938787
80-81	22.318020277116673	27.850216524270678	27.416164822435313	22.415598376177336
82-83	22.349870816156	27.837852023778353	27.492248854757356	22.32002830530829
84-85	22.414658943305774	27.8149924401149	27.441079277559226	22.329269339020094
86-87	22.267621701330633	27.86106442910673	27.50364736082159	22.36766650874105
88-89	22.36618068074891	27.771490965898998	27.46744390092917	22.39488445242292
90-91	22.38155401595745	27.789656109065263	27.47377756293429	22.355012312042987
92-93	22.38812920379898	27.780469564581484	27.50224265466037	22.32915857695917
94-95	22.279680336076186	27.861417266110873	27.5001540421451	22.358748355667846
96-97	22.277176619207047	27.845264447599092	27.532019562316695	22.345539370877162
98-99	22.502154789104292	28.409581744374407	27.669419904654468	21.418843561866836
100-101	26.653703874088762	35.42275333043285	28.219399155838783	9.704143639639607
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1619.0
1	1431.5
2	1855.0
3	2593.0
4	3471.5
5	5506.0
6	7181.0
7	8265.5
8	8937.5
9	8729.5
10	7976.0
11	7287.0
12	7849.0
13	10296.0
14	15922.0
15	23498.0
16	29796.5
17	33540.0
18	34641.5
19	33890.5
20	32515.5
21	31698.0
22	32911.0
23	37539.5
24	46153.5
25	59052.0
26	75780.0
27	94163.5
28	113698.5
29	133385.5
30	151182.0
31	168992.0
32	187236.0
33	205428.0
34	224314.5
35	242710.5
36	263669.0
37	282965.0
38	298455.0
39	312701.5
40	326284.5
41	340056.0
42	350191.0
43	358106.0
44	366829.5
45	373907.0
46	376825.0
47	377914.0
48	376021.5
49	370105.0
50	362118.0
51	349741.0
52	332052.0
53	312018.5
54	290942.5
55	268706.5
56	246238.5
57	222399.0
58	197088.5
59	170326.5
60	142213.5
61	115803.5
62	91987.0
63	71196.0
64	53376.5
65	39800.0
66	30195.0
67	23118.5
68	17667.5
69	12800.5
70	9059.0
71	6474.0
72	4514.5
73	3061.0
74	2053.5
75	1363.5
76	956.5
77	685.5
78	497.0
79	372.0
80	263.5
81	181.0
82	139.0
83	96.0
84	63.0
85	51.0
86	42.0
87	30.5
88	21.5
89	14.0
90	9.0
91	7.0
92	4.5
93	3.0
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004415960387775491
3	1.1775894367401309E-5
4	2.3551788734802617E-5
5	9.420715493921047E-5
6	0.0
7	0.0
8	4.8281166906345366E-4
9	1.2953483804141442E-4
10-11	2.3551788734802617E-5
12-13	2.9439735918503274E-4
14-15	5.887947183700654E-6
16-17	5.416911409004603E-4
18-19	0.0
20-21	6.476949361561213E-5
22-23	0.0
24-25	0.0
26-27	5.8920686157315406E-5
28-29	5.896026172696022E-6
30-31	8.85696654446218E-5
32-33	3.9671418809214407E-4
34-35	0.0
36-37	3.643009658693592E-4
38-39	0.0011913255614783556
40-41	0.0
42-43	0.0
44-45	3.2737789700098375E-4
46-47	0.0
48-49	0.0
50-51	3.827784162734416E-5
52-53	5.797302347739973E-4
54-55	0.003252186721568782
56-57	0.003825750349937835
58-59	0.004279796337517765
60-61	0.001101892836890297
62-63	1.3581933420819094E-5
64-65	6.313542026092497E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	2.875710084712668E-4
74-75	2.908962914722661E-5
76-77	0.0
78-79	0.0
80-81	6.184860968850928E-4
82-83	0.0
84-85	0.0
86-87	2.3508604345095343E-5
88-89	0.0
90-91	0.0011374886573826079
92-93	2.0495796599058686E-4
94-95	8.337869134142308E-6
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	162.0
20-21	585.0
22-23	1196.0
24-25	3058.0
26-27	4724.0
28-29	9065.0
30-31	22572.0
32-33	27388.0
34-35	37515.0
36-37	58316.0
38-39	68541.0
40-41	67689.0
42-43	75141.0
44-45	88260.0
46-47	87202.0
48-49	83536.0
50-51	76599.0
52-53	73571.0
54-55	80040.0
56-57	82510.0
58-59	82029.0
60-61	80410.0
62-63	76547.0
64-65	79493.0
66-67	83446.0
68-69	84498.0
70-71	83051.0
72-73	79392.0
74-75	81692.0
76-77	79353.0
78-79	83848.0
80-81	85571.0
82-83	81967.0
84-85	81420.0
86-87	85451.0
88-89	91568.0
90-91	101625.0
92-93	98950.0
94-95	113292.0
96-97	175398.0
98-99	714819.0
100-101	5020434.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.02932572243046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.9501558565284	79.15330738543625
2	6.48898024734722	11.29463151105597
3	1.4786426173839569	3.8605580992612647
4	0.5007980613107937	1.7433647039591504
5	0.2259128696025582	0.9830522356764997
6	0.11946048255972487	0.623793914858941
7	0.06336127077978179	0.3860002071016521
8	0.04483778392933254	0.3121761681806271
9	0.030940095300547876	0.2423426068614979
>10	0.09491698851009185	1.2541075970751074
>50	0.0017504941139982813	0.10145373701553775
>100	2.2276035869780623E-4	0.03452170383568085
>500	2.0472274896997025E-5	0.010690129681739343
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	4.710357746960524E-5	0.0	0.0	0.0
5	0.0	4.710357746960524E-5	0.0	0.0	0.0
6	0.0	4.710357746960524E-5	0.0	0.0	0.0
7	0.0	5.887947183700655E-5	0.0	0.0	0.0
8	0.0	7.065536620440786E-5	0.0	0.0	0.0
9	0.0	1.177589436740131E-4	0.0	2.355178873480262E-5	0.0
10-11	0.0	1.177589436740131E-4	0.0	2.355178873480262E-5	0.0
12-13	0.0	1.177589436740131E-4	0.0	3.532768310220393E-5	0.0
14-15	0.0	2.1196609861322355E-4	0.0	5.887947183700655E-5	0.0
16-17	1.177589436740131E-5	2.2374199298062487E-4	0.0	5.887947183700655E-5	0.0
18-19	1.177589436740131E-5	2.6495762326652944E-4	0.0	6.476741902070721E-5	5.887947183700655E-6
20-21	1.177589436740131E-5	3.0617325355243406E-4	0.0	9.420715493921048E-5	2.355178873480262E-5
22-23	1.7663841551101967E-5	3.2383709510353605E-4	0.0	1.1187099649031244E-4	4.121563028590458E-5
24-25	2.355178873480262E-5	3.4150093665463797E-4	0.0	1.4719867959251637E-4	7.065536620440786E-5
26-27	2.355178873480262E-5	3.4150093665463797E-4	0.0	2.1785404579692421E-4	7.65433133881085E-5
28-29	2.355178873480262E-5	3.4738888383833863E-4	0.0	6.358982958396708E-4	8.243126057180916E-5
30-31	2.355178873480262E-5	4.7692372187975305E-4	0.0	0.002196204299520344	8.831920775550982E-5
32-33	2.355178873480262E-5	4.828116690634537E-4	0.0	0.005369807831534998	9.420715493921048E-5
34-35	2.355178873480262E-5	5.475790880841609E-4	0.0	0.009226413236858926	1.0598304930661179E-4
36-37	2.355178873480262E-5	6.123465071048681E-4	0.0	0.015538292617786028	1.0598304930661179E-4
38-39	2.355178873480262E-5	6.123465071048681E-4	0.0	0.02542415593921943	1.177589436740131E-4
40-41	2.355178873480262E-5	7.065536620440786E-4	0.0	0.03468000891199686	1.2953483804141442E-4
42-43	2.355178873480262E-5	7.183295564114799E-4	0.0	0.042528642507869835	1.3542278522511508E-4
44-45	2.355178873480262E-5	8.066487641669898E-4	0.0	0.051136821290440185	1.4719867959251637E-4
46-47	2.355178873480262E-5	8.831920775550983E-4	0.0	0.060963805140036575	1.6486252114361833E-4
48-49	2.355178873480262E-5	8.890800247387989E-4	0.0	0.0702255460599977	1.6486252114361833E-4
50-51	3.532768310220393E-5	9.538474437595061E-4	0.0	0.07885138868411917	1.6486252114361833E-4
52-53	3.532768310220393E-5	9.656233381269074E-4	0.0	0.08774807687869085	1.8841430987842097E-4
54-55	3.532768310220393E-5	9.71511285310608E-4	0.0	0.09645635076338413	2.1785404579692421E-4
56-57	3.532768310220393E-5	9.773992324943086E-4	0.0	0.10417544952121569	2.355178873480262E-4
58-59	3.532768310220393E-5	9.950630740454107E-4	0.0	0.11121154640573797	2.472937817154275E-4
60-61	3.532768310220393E-5	0.0010362787043313153	0.0	0.11824175534307654	2.472937817154275E-4
62-63	3.532768310220393E-5	0.0010716063874335192	0.0	0.12490691155502569	2.6495762326652944E-4
64-65	3.532768310220393E-5	0.0011893653311075322	0.0	0.13153674008387262	2.708455704502301E-4
66-67	3.532768310220393E-5	0.0011893653311075322	0.0	0.13803703377467813	2.7673351763393076E-4
68-69	3.532768310220393E-5	0.0012188050670260357	0.0	0.14462564667323918	3.002853063687334E-4
70-71	3.532768310220393E-5	0.001242356855760838	0.0	0.15074911174428784	3.0617325355243406E-4
72-73	3.532768310220393E-5	0.001248244802944539	0.0	0.15603060036806735	3.0617325355243406E-4
74-75	3.532768310220393E-5	0.0012600206973119401	0.0	0.16041712101992434	3.120612007361347E-4
76-77	3.532768310220393E-5	0.0013071242747815454	0.0	0.16450924431259628	3.2383709510353605E-4
78-79	3.532768310220393E-5	0.0013542278522511507	0.0	0.16826575461579732	3.2972504228723665E-4
80-81	3.532768310220393E-5	0.0013542278522511507	0.0	0.16994970751033572	3.4150093665463797E-4
82-83	3.532768310220393E-5	0.0013601157994348513	0.0	0.17060326964772649	3.650527253894406E-4
84-85	3.532768310220393E-5	0.0013895555353533547	0.0	0.17111552105270844	3.650527253894406E-4
86-87	3.532768310220393E-5	0.001401331429720756	0.0	0.17135103894005646	3.650527253894406E-4
88-89	3.532768310220393E-5	0.001448435007190361	0.0	0.17139814251752605	3.8271656694054254E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8305	0.0	42.742653	1
GTATCAA	14390	0.0	34.7917	1
TCAACGC	16630	0.0	29.047146	4
ATCAACG	16815	0.0	28.827143	3
CAACGCA	17075	0.0	28.26562	5
AACGCAG	17710	0.0	27.228668	6
TATCAAC	18115	0.0	26.990425	2
ACGCAGA	20720	0.0	23.293352	7
CGCAGAG	20995	0.0	22.968311	8
GTACATG	22045	0.0	21.11543	1
TACATGG	21905	0.0	20.39043	2
ACATGGG	22340	0.0	19.730364	3
GCAGAGT	26045	0.0	18.402363	9
CATGGGG	16410	0.0	17.626226	4
GAGTACT	13655	0.0	17.396637	12-13
AGAGTAC	22690	0.0	17.175432	10-11
CAGAGTA	25270	0.0	16.32465	10-11
GTGGTAT	4885	0.0	16.281494	1
TATACCG	655	0.0	15.976756	5
GTACTTT	15825	0.0	15.170281	14-15
>>END_MODULE