FastQCFastQC Report
Fri 27 May 2016
SRR2138796_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138796_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12041066
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126000.043.476441
GTATCAA213650.034.1207471
TCAACGC248800.028.531464
ATCAACG250350.028.3039673
CAACGCA254600.027.8148275
AACGCAG265950.026.7076516
TATCAAC269400.026.6659852
ACGCAGA306600.023.1389887
CGCAGAG310900.022.791668
GTACATG331550.021.6929661
TACATGG330900.021.2482912
ACATGGG337050.020.318313
GCAGAGT389950.018.1060879
CATGGGG241300.018.0764944
AGAGTAC337950.017.27619210-11
GAGTACT204800.017.1443512-13
GTGGTAT72050.016.7845291
CAGAGTA377550.016.48684910-11
GTACTTT236600.014.95714414-15
TGGTATC72550.014.8557492
Produced by FastQC (version 0.11.5)