##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138796_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12041066
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33952874272095	33.0	31.0	34.0	31.0	34.0
2	32.50767157990829	34.0	31.0	34.0	31.0	34.0
3	32.626723497736826	34.0	31.0	34.0	31.0	34.0
4	36.14735530890704	37.0	35.0	37.0	35.0	37.0
5	35.983642727313345	37.0	35.0	37.0	35.0	37.0
6	36.02771897438316	37.0	35.0	37.0	35.0	37.0
7	36.0043325898222	37.0	35.0	37.0	35.0	37.0
8	35.986617214788126	37.0	35.0	37.0	35.0	37.0
9	37.82233267386791	39.0	38.0	39.0	35.0	39.0
10-11	37.69867107281033	39.0	37.0	39.0	35.0	39.0
12-13	37.72065533898743	39.0	37.0	39.0	35.0	39.0
14-15	39.04637558667979	40.0	38.0	41.0	36.0	41.0
16-17	38.99866589884982	40.0	38.0	41.0	36.0	41.0
18-19	39.05508478236063	40.0	38.5	41.0	36.0	41.0
20-21	39.050085180200234	40.0	39.0	41.0	36.0	41.0
22-23	39.01438822642136	40.0	39.0	41.0	36.0	41.0
24-25	39.001539485593746	40.0	38.5	41.0	36.0	41.0
26-27	38.90918171593906	40.0	38.0	41.0	35.0	41.0
28-29	38.828915751041144	40.0	38.0	41.0	35.0	41.0
30-31	38.68797075065167	40.0	38.0	41.0	35.0	41.0
32-33	38.60365960053499	40.0	38.0	41.0	35.0	41.0
34-35	38.56331545064784	40.0	38.0	41.0	35.0	41.0
36-37	38.465104356901264	40.0	38.0	41.0	34.0	41.0
38-39	38.43034841536323	40.0	38.0	41.0	34.0	41.0
40-41	38.42507325651988	40.0	38.0	41.0	34.0	41.0
42-43	38.38444166535694	40.0	38.0	41.0	34.0	41.0
44-45	38.27789710003825	40.0	38.0	41.0	34.0	41.0
46-47	38.2194543101536	40.0	38.0	41.0	34.0	41.0
48-49	38.19453371865603	40.0	38.0	41.0	33.0	41.0
50-51	38.1134990298285	40.0	37.5	41.0	33.0	41.0
52-53	37.97545885599136	40.0	37.0	41.0	33.0	41.0
54-55	37.795927105731785	39.5	37.0	41.0	33.0	41.0
56-57	37.670963074282284	39.0	36.5	41.0	33.0	41.0
58-59	37.44502893740254	39.0	36.0	41.0	32.5	41.0
60-61	37.274436479735115	39.0	36.0	40.0	32.0	41.0
62-63	37.053225376427626	39.0	35.0	40.0	31.5	41.0
64-65	36.725869191209725	38.0	35.0	40.0	31.0	41.0
66-67	36.64905526843562	38.0	35.0	40.0	31.0	41.0
68-69	36.41213420478614	37.0	35.0	40.0	31.0	41.0
70-71	36.09671041500315	37.0	35.0	39.0	31.0	41.0
72-73	35.77798175647193	36.0	34.0	39.0	31.0	41.0
74-75	35.37725572337408	36.0	34.0	39.0	30.5	40.0
76-77	33.33553027499369	34.5	32.0	36.5	27.5	39.0
78-79	34.52915104308289	35.0	33.5	37.0	30.0	39.0
80-81	34.54150723818383	35.0	34.0	37.0	30.0	39.0
82-83	34.259402342109915	35.0	34.0	36.0	30.0	38.5
84-85	34.00852001901431	35.0	34.0	36.0	30.0	37.0
86-87	33.8067558514213	35.0	34.0	35.5	30.0	37.0
88-89	33.57419176295869	35.0	33.0	35.0	29.5	36.5
90-91	33.416841362411986	35.0	33.0	35.0	29.0	36.0
92-93	33.356285231736464	35.0	33.0	35.0	29.0	36.0
94-95	33.33630369585614	35.0	33.0	35.0	29.0	36.0
96-97	33.37423703992273	35.0	33.0	35.0	30.0	35.5
98-99	33.406905363202185	35.0	33.0	35.0	30.0	35.0
100-101	32.12944126150496	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	21.0
20	45.0
21	206.0
22	1032.0
23	3325.0
24	9257.0
25	20425.0
26	38433.0
27	63317.0
28	94463.0
29	132615.0
30	177344.0
31	230807.0
32	303380.0
33	411258.0
34	597621.0
35	919810.0
36	1625623.0
37	2916746.0
38	3258609.0
39	1220485.0
40	16243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5957690124778	20.39495506460973	14.081734956024658	23.927540966887815
2	14.566087097350206	21.104384766476613	41.9213075814643	22.40822055470888
3	18.499351386007547	25.921355761037653	30.143060494674057	25.436232358280748
4	12.525859438597506	17.91573464723781	39.01474438541862	30.543661528746064
5	13.201910795228992	37.92570587639294	35.73932859731618	13.133054731061883
6	30.126311075780166	38.10045555767238	18.774160028688488	12.999073337858958
7	26.735608519678284	32.40357891930656	23.223618508827915	17.637194052187244
8	24.11493007323462	37.63040624611225	21.07221593907363	17.182447741579495
9	25.428110291987682	17.061501914782227	21.26597534302639	36.244412450203704
10-11	24.178800393669707	26.941132293311256	29.0185783915924	19.861488921426634
12-13	24.921040794855724	24.28513163494014	29.269099781181474	21.524727789022666
14-15	21.984911633647023	25.82949680945996	26.61861305457615	25.56697850231686
16-17	21.769691422990494	29.00696142491517	27.270257302187794	21.953089849906537
18-19	21.815665806969733	28.320389433991096	28.777657956335258	21.086286802703913
20-21	22.529842033587354	27.482140250654187	28.651877788590646	21.33613992716781
22-23	22.257983627625705	27.393518477373675	28.532868547653834	21.815629347346785
24-25	22.02942075029165	27.514339339479672	28.55450610510995	21.901733805118734
26-27	22.000128727503697	27.705092352842502	28.457786748720963	21.836992170932838
28-29	21.95953055029206	27.767105265948032	28.47721225037767	21.796151933382237
30-31	22.23172765411071	27.59075674002452	28.388116665067237	21.78939894079753
32-33	21.99577227967422	27.77521611740231	28.334936475065458	21.894075127858013
34-35	22.101419264660375	27.769056517159	28.365353848495108	21.764170369685512
36-37	22.142549425168408	27.727505078843073	28.286433172361964	21.843512323626555
38-39	21.980807556646	27.845069841077013	28.364603634947322	21.80951896732967
40-41	22.18352224386831	27.695487429734705	28.270981695793463	21.850008630603522
42-43	22.113274623392797	27.756612807498716	28.13243682015335	21.99767574895514
44-45	22.165904183383866	27.846870730512087	27.989645869516654	21.997579216587386
46-47	22.196702984653406	27.84507424962854	27.801222472134118	22.157000293583934
48-49	22.15514197570596	27.936099520509778	27.79781135189366	22.110947151890603
50-51	22.16142318410604	27.98008928299682	27.72247790506738	22.136009627829765
52-53	22.31111109113339	27.926653497852854	27.59798903268103	22.164246378332724
54-55	22.269067468536125	27.83541271178928	27.64116205775374	22.254357761920858
56-57	22.25847565101795	27.928097089875386	27.6363810642206	22.17704619488606
58-59	22.276201897318757	27.973465014689523	27.60048320063283	22.14984988735889
60-61	22.32604812681758	27.91408455155851	27.623444434137173	22.136422887486738
62-63	22.305642403123514	27.964824165615116	27.575954810160948	22.15357862110043
64-65	22.299260670805722	27.931497662856064	27.52962105499262	22.239620611345597
66-67	22.33813701762627	27.928221919327033	27.47158530237647	22.262055760670226
68-69	22.24252671847716	27.967535858660224	27.526337605816977	22.263599817045645
70-71	22.278621139861123	27.747279432222495	27.591890746677755	22.382208681238627
72-73	22.30484250700821	27.798082426036157	27.559238127844825	22.337836939110808
74-75	22.1728041065347	27.929509030102217	27.540114623474942	22.35757223988814
76-77	22.32764331748689	27.862730952551157	27.450054793667256	22.359570936294695
78-79	22.269911337328416	27.893564718252673	27.42921924801785	22.407304696401063
80-81	22.265074735770472	27.90111684276769	27.459133563637284	22.374674857824562
82-83	22.296965205375283	27.86786257545296	27.549134996966117	22.28603722220564
84-85	22.368421963889386	27.83844183012274	27.491876185702374	22.301260020285497
86-87	22.2413553959181	27.87541562567006	27.553803220254963	22.329425758156876
88-89	22.315020854557087	27.86516566603115	27.44545172196809	22.37436175744368
90-91	22.331255888389137	27.8575330068645	27.49638586686792	22.314825237878445
92-93	22.3104610419343	27.866453757946125	27.50315558188908	22.319929618230496
94-95	22.21335393006594	27.87866366786303	27.548731131782667	22.359251270288365
96-97	22.195982176012315	27.920459002917585	27.560836754404676	22.322722066665417
98-99	22.41772443375678	28.447361116420193	27.68950477887408	21.445409670948944
100-101	23.219176190901052	31.013734129378808	24.753368038562517	21.013721641157627
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2421.0
1	2059.0
2	2595.5
3	3723.0
4	5095.0
5	8176.5
6	10734.5
7	12511.5
8	13699.5
9	13412.5
10	12194.5
11	11084.5
12	11890.0
13	15547.5
14	24367.0
15	36374.0
16	45757.0
17	51148.0
18	52767.5
19	51208.5
20	48581.0
21	46811.0
22	47914.5
23	54233.5
24	66510.5
25	84492.0
26	107701.5
27	133484.5
28	161147.0
29	189207.0
30	214857.5
31	239929.0
32	265187.5
33	289816.5
34	314354.0
35	338417.5
36	364341.5
37	389234.5
38	410619.0
39	427543.0
40	444011.0
41	462967.0
42	477601.0
43	490076.5
44	502994.5
45	513295.0
46	517995.0
47	520189.0
48	520721.5
49	514997.5
50	503982.0
51	487807.0
52	463947.5
53	437426.0
54	411040.0
55	380799.0
56	348266.5
57	314218.5
58	277591.5
59	239381.5
60	199503.0
61	162078.5
62	128897.0
63	99986.0
64	75431.5
65	55711.5
66	41832.0
67	32064.0
68	24612.5
69	18098.5
70	13120.5
71	9586.5
72	6805.5
73	4698.5
74	3298.5
75	2368.0
76	1618.0
77	1067.0
78	740.5
79	543.0
80	402.5
81	281.5
82	202.5
83	142.0
84	96.5
85	69.5
86	52.5
87	42.0
88	30.0
89	21.5
90	16.0
91	10.0
92	8.5
93	6.5
94	3.5
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004285334869852885
3	4.98294752308475E-5
4	6.643930030779667E-5
5	4.98294752308475E-5
6	0.0
7	8.304912538474584E-6
8	3.3219650153898335E-4
9	8.304912538474584E-5
10-11	6.228684403855937E-5
12-13	3.155866764620342E-4
14-15	8.304912538474584E-6
16-17	5.688865088855089E-4
18-19	8.304912538474584E-6
20-21	4.983099610043387E-5
22-23	0.0
24-25	0.0
26-27	7.063802508048787E-5
28-29	4.157851328874232E-6
30-31	6.661384466342543E-5
32-33	3.9228096230860184E-4
34-35	0.0
36-37	4.077265269736738E-4
38-39	0.0011547412002904534
40-41	0.0
42-43	0.0
44-45	3.4631974663247334E-4
46-47	0.0
48-49	8.829251805670287E-6
50-51	8.466722083590788E-5
52-53	5.034360182490163E-4
54-55	0.0032782889127452803
56-57	0.003419288540806027
58-59	0.004215716356988711
60-61	9.103721358525461E-4
62-63	4.714424890843384E-6
64-65	6.140570219062701E-4
66-67	0.0
68-69	0.0
70-71	9.831303677074707E-6
72-73	1.7396853187608014E-4
74-75	4.522347633063547E-5
76-77	0.0
78-79	0.0
80-81	5.199959773111195E-4
82-83	0.0
84-85	0.0
86-87	5.392833108155587E-6
88-89	0.0
90-91	0.0010868621325366263
92-93	2.140423135743551E-4
94-95	1.717921129897342E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	199.0
20-21	829.0
22-23	1795.0
24-25	3861.0
26-27	6364.0
28-29	12131.0
30-31	27177.0
32-33	32580.0
34-35	45119.0
36-37	69035.0
38-39	84051.0
40-41	84294.0
42-43	96587.0
44-45	112166.0
46-47	111791.0
48-49	107648.0
50-51	98307.0
52-53	95016.0
54-55	102486.0
56-57	106159.0
58-59	106644.0
60-61	105845.0
62-63	101518.0
64-65	104466.0
66-67	111156.0
68-69	113804.0
70-71	113534.0
72-73	108737.0
74-75	110554.0
76-77	107223.0
78-79	115155.0
80-81	117429.0
82-83	113818.0
84-85	113002.0
86-87	118685.0
88-89	127852.0
90-91	142875.0
92-93	141035.0
94-95	162239.0
96-97	252572.0
98-99	1031741.0
100-101	7323587.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.27121919785242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.7072093449059	76.49443111677105
2	7.20233141255779	12.283031612315867
3	1.780515888673858	4.554802818851026
4	0.6207108230486579	2.11715074602646
5	0.2815855537297146	1.2005571737517573
6	0.13409666405153525	0.6860751620423544
7	0.0865645665504673	0.5167026290364386
8	0.048591737668288705	0.33147813711337504
9	0.034045769965637095	0.2612811882149551
>10	0.10120989531684942	1.3273379866174317
>50	0.002644565239908411	0.15189588764861847
>100	4.7809933076370544E-4	0.06756799812515417
>500	1.5678960580336713E-5	0.007687543485532181
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.304912538474584E-6
2	0.0	1.660982507694917E-5	0.0	8.304912538474584E-6	2.491473761542375E-5
3	0.0	1.660982507694917E-5	0.0	1.660982507694917E-5	2.491473761542375E-5
4	0.0	5.813438776932209E-5	0.0	2.491473761542375E-5	2.491473761542375E-5
5	0.0	6.643930030779667E-5	0.0	3.321965015389834E-5	2.491473761542375E-5
6	1.660982507694917E-5	6.643930030779667E-5	0.0	3.321965015389834E-5	4.152456269237292E-5
7	1.660982507694917E-5	6.643930030779667E-5	0.0	4.98294752308475E-5	4.152456269237292E-5
8	1.660982507694917E-5	6.643930030779667E-5	0.0	6.643930030779667E-5	4.98294752308475E-5
9	1.660982507694917E-5	1.4118351315406792E-4	0.0	9.9658950461695E-5	5.813438776932209E-5
10-11	3.321965015389834E-5	1.4118351315406792E-4	0.0	1.2872614434635606E-4	5.813438776932209E-5
12-13	4.152456269237292E-5	1.4118351315406792E-4	0.0	1.5364088196177979E-4	6.228684403855939E-5
14-15	4.152456269237292E-5	1.7025070703872896E-4	0.0	1.8270807584644085E-4	7.889666911550854E-5
16-17	5.813438776932209E-5	1.7855561957720356E-4	0.0	1.9931790092339E-4	9.135403792322042E-5
18-19	7.474421284627125E-5	1.951654446541527E-4	0.0	2.0762281346186457E-4	9.135403792322042E-5
20-21	7.889666911550854E-5	2.076228134618646E-4	0.0	2.4084246361576292E-4	9.135403792322042E-5
22-23	8.304912538474584E-5	2.3253755107728835E-4	0.0	2.657572012311867E-4	1.2872614434635606E-4
24-25	9.9658950461695E-5	2.3669000734652564E-4	0.0	2.8651948257737313E-4	1.6609825076949167E-4
26-27	9.9658950461695E-5	2.4084246361576292E-4	0.0	3.3634895780822063E-4	1.7440316330796627E-4
28-29	9.9658950461695E-5	2.4084246361576292E-4	0.0	6.560880905394921E-4	1.8270807584644085E-4
30-31	1.0796386300016959E-4	2.8236702630813585E-4	0.0	0.0015447137321562725	1.8686053211567814E-4
32-33	1.1626877553864417E-4	2.98976851385085E-4	0.0	0.003259678171351274	2.2008018226957646E-4
34-35	1.2042123180788146E-4	3.446538703466952E-4	0.0	0.005344211218508395	2.4084246361576292E-4
36-37	1.2457368807711875E-4	3.944833455775427E-4	0.0	0.008670328690167465	2.4084246361576292E-4
38-39	1.2457368807711875E-4	3.9863580184678E-4	0.0	0.01396471043344501	2.4084246361576292E-4
40-41	1.3287860061559335E-4	4.567701896161021E-4	0.0	0.01954976411556917	2.4084246361576292E-4
42-43	1.3287860061559335E-4	4.609226458853394E-4	0.0	0.024707114801961888	2.4084246361576292E-4
44-45	1.3287860061559335E-4	4.941422960392378E-4	0.0	0.030487333928740198	2.449949198850002E-4
46-47	1.3287860061559335E-4	5.232094899238987E-4	0.0	0.03689872640844258	2.574522886927121E-4
48-49	1.3287860061559335E-4	5.232094899238987E-4	0.0	0.04283258641718267	2.906719388466104E-4
50-51	1.3287860061559335E-4	6.062586153086446E-4	0.0	0.04889102011399987	2.906719388466104E-4
52-53	1.3703105688483064E-4	6.145635278471191E-4	0.0	0.05512385697412505	3.2804404526974606E-4
54-55	1.4118351315406792E-4	6.228684403855938E-4	0.0	0.06128194962140395	3.5711123915440707E-4
56-57	1.4118351315406792E-4	6.477831780010175E-4	0.0	0.06747326191883676	3.6126369542364436E-4
58-59	1.4118351315406792E-4	6.560880905394921E-4	0.0	0.07320365157038422	3.69568607962119E-4
60-61	1.494884256925425E-4	6.602405468087295E-4	0.0	0.07905030999747031	3.8202597676983085E-4
62-63	1.5364088196177979E-4	6.810028281549158E-4	0.0	0.08457307683555593	3.903308893083054E-4
64-65	1.577933382310171E-4	7.640519535396617E-4	0.0	0.09032838122471881	3.944833455775427E-4
66-67	1.577933382310171E-4	7.889666911550854E-4	0.0	0.09628715597107432	4.027882581160173E-4
68-69	1.577933382310171E-4	7.9727160369356E-4	0.0	0.10196771614739093	4.0694071438525457E-4
70-71	1.6194579450025439E-4	8.138814287705091E-4	0.0	0.10793894826255417	4.193980831929665E-4
72-73	1.6609825076949167E-4	8.221863413089838E-4	0.0	0.11281393192263875	5.149045773854241E-4
74-75	1.6609825076949167E-4	8.221863413089838E-4	0.0	0.11692901608545289	5.149045773854241E-4
76-77	1.6609825076949167E-4	8.38796166385933E-4	0.0	0.12069114146538189	5.190570336546614E-4
78-79	1.7440316330796625E-4	8.471010789244075E-4	0.0	0.1239217524428485	5.232094899238987E-4
80-81	1.7440316330796625E-4	8.471010789244075E-4	0.0	0.12541248424350468	5.522766838085598E-4
82-83	1.7440316330796625E-4	8.471010789244075E-4	0.0	0.12609763952792885	5.813438776932208E-4
84-85	1.7440316330796625E-4	8.471010789244075E-4	0.0	0.12679109972489147	5.813438776932208E-4
86-87	1.7440316330796625E-4	8.471010789244075E-4	0.0	0.1271066864013535	5.854963339624582E-4
88-89	1.7440316330796625E-4	8.96930554155255E-4	0.0	0.1272063453518152	5.896487902316954E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12600	0.0	43.47644	1
GTATCAA	21365	0.0	34.120747	1
TCAACGC	24880	0.0	28.53146	4
ATCAACG	25035	0.0	28.303967	3
CAACGCA	25460	0.0	27.814827	5
AACGCAG	26595	0.0	26.707651	6
TATCAAC	26940	0.0	26.665985	2
ACGCAGA	30660	0.0	23.138988	7
CGCAGAG	31090	0.0	22.79166	8
GTACATG	33155	0.0	21.692966	1
TACATGG	33090	0.0	21.248291	2
ACATGGG	33705	0.0	20.31831	3
GCAGAGT	38995	0.0	18.106087	9
CATGGGG	24130	0.0	18.076494	4
AGAGTAC	33795	0.0	17.276192	10-11
GAGTACT	20480	0.0	17.14435	12-13
GTGGTAT	7205	0.0	16.784529	1
CAGAGTA	37755	0.0	16.486849	10-11
GTACTTT	23660	0.0	14.957144	14-15
TGGTATC	7255	0.0	14.855749	2
>>END_MODULE