FastQCFastQC Report
Fri 27 May 2016
SRR2138795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10302682
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113950.044.8241731
GTATCAA200300.034.8096281
TCAACGC235150.028.7689574
ATCAACG237050.028.5383683
CAACGCA241000.028.088175
AACGCAG246700.027.4737346
TATCAAC251550.027.398392
ACGCAGA289050.023.419187
CGCAGAG292750.023.1231928
GTACATG285850.021.3889641
TACATGG285700.020.911912
ACATGGG284900.020.2576623
GCAGAGT351800.019.1817519
CATGGGG206350.018.5435644
GAGTACT199850.017.98337712-13
AGAGTAC314050.017.59665510-11
GTGGTAT65250.017.4961681
CAGAGTA343850.017.0799510-11
TGGTATC62900.015.9988242
GTACTTT233400.015.4441414-15
Produced by FastQC (version 0.11.5)