##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138795_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10302682
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.340359238497314	33.0	31.0	34.0	31.0	34.0
2	32.502286297878555	34.0	31.0	34.0	31.0	34.0
3	32.61920371802216	34.0	31.0	34.0	31.0	34.0
4	36.14402201290887	37.0	35.0	37.0	35.0	37.0
5	35.98318777576557	37.0	35.0	37.0	35.0	37.0
6	36.02742179172375	37.0	35.0	37.0	35.0	37.0
7	36.001721105242304	37.0	35.0	37.0	35.0	37.0
8	35.98714858907613	37.0	35.0	37.0	35.0	37.0
9	37.81822199306938	39.0	38.0	39.0	35.0	39.0
10-11	37.697453633917846	39.0	37.0	39.0	35.0	39.0
12-13	37.717340494446006	39.0	37.0	39.0	35.0	39.0
14-15	39.037875526003816	40.0	38.0	41.0	36.0	41.0
16-17	38.99189788639502	40.0	38.0	41.0	36.0	41.0
18-19	39.05158583949306	40.0	38.5	41.0	36.0	41.0
20-21	39.04900268508531	40.0	39.0	41.0	36.0	41.0
22-23	39.013214054567335	40.0	39.0	41.0	36.0	41.0
24-25	38.99873832873437	40.0	38.0	41.0	36.0	41.0
26-27	38.90290674441715	40.0	38.0	41.0	35.0	41.0
28-29	38.82116552403318	40.0	38.0	41.0	35.0	41.0
30-31	38.67831571221899	40.0	38.0	41.0	35.0	41.0
32-33	38.590558729295154	40.0	38.0	41.0	35.0	41.0
34-35	38.54734952839141	40.0	38.0	41.0	35.0	41.0
36-37	38.44889992073572	40.0	38.0	41.0	34.0	41.0
38-39	38.412362426101524	40.0	38.0	41.0	34.0	41.0
40-41	38.4069104968771	40.0	38.0	41.0	34.0	41.0
42-43	38.365545744953764	40.0	38.0	41.0	34.0	41.0
44-45	38.2558909231372	40.0	38.0	41.0	34.0	41.0
46-47	38.19498795745713	40.0	38.0	41.0	33.0	41.0
48-49	38.17175761104241	40.0	38.0	41.0	33.0	41.0
50-51	38.091369344615465	40.0	37.0	41.0	33.0	41.0
52-53	37.95301551849229	40.0	37.0	41.0	33.0	41.0
54-55	37.774259449802116	39.5	37.0	41.0	33.0	41.0
56-57	37.64675157060858	39.0	36.0	41.0	33.0	41.0
58-59	37.420556006278034	39.0	36.0	41.0	32.5	41.0
60-61	37.25164230042415	39.0	35.5	40.0	32.0	41.0
62-63	37.03079710390995	39.0	35.0	40.0	31.5	41.0
64-65	36.70553221562456	38.0	35.0	40.0	31.0	41.0
66-67	36.633494402103295	38.0	35.0	40.0	31.0	41.0
68-69	36.39774107051984	37.0	35.0	40.0	31.0	41.0
70-71	36.08705637568856	37.0	35.0	39.5	31.0	41.0
72-73	35.77640113595113	36.0	34.0	39.0	31.0	41.0
74-75	35.37785980506261	36.0	34.0	39.0	30.5	40.0
76-77	33.34028742901182	34.5	32.0	36.5	27.5	39.0
78-79	34.53777099202679	35.0	33.5	37.0	30.0	39.0
80-81	34.55398852221189	35.0	34.0	37.0	30.0	39.0
82-83	34.27407957183539	35.0	34.0	36.0	30.0	38.5
84-85	34.02077250266606	35.0	34.0	36.0	30.0	37.0
86-87	33.81629144741929	35.0	34.0	35.5	30.0	37.0
88-89	33.58425660264335	35.0	33.0	35.0	29.5	36.5
90-91	33.423710141542486	35.0	33.0	35.0	29.0	36.0
92-93	33.36177574383214	35.0	33.0	35.0	29.0	36.0
94-95	33.339236118793224	35.0	33.0	35.0	29.0	36.0
96-97	33.37725561450849	35.0	33.0	35.0	30.0	35.5
98-99	33.40851202710609	35.0	33.0	35.0	30.0	35.0
100-101	32.13566198379098	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	9.0
20	42.0
21	189.0
22	871.0
23	2929.0
24	8125.0
25	17581.0
26	32719.0
27	54506.0
28	81025.0
29	113098.0
30	151159.0
31	198984.0
32	261477.0
33	353893.0
34	514295.0
35	792393.0
36	1394741.0
37	2478741.0
38	2767393.0
39	1063804.0
40	14701.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5435611814477	20.47463951619588	14.19271215009839	23.789087152258023
2	14.52453215963298	21.139758840608522	42.096042876922205	22.239666122836294
3	18.36726691201393	26.12084472034256	30.259633516573754	25.25225485106976
4	12.48690024932355	17.99399321166722	39.006134036813926	30.5129725021953
5	13.156916962900498	37.872874143717276	35.77146782892826	13.198741064453959
6	29.98884174043225	38.149192608293646	18.861147029482225	13.000818621791879
7	26.562292529710717	32.49931085892214	23.37493739493025	17.56345921643689
8	23.987686651188433	37.72494234487471	21.130758718153793	17.156612285783062
9	25.358807369699104	17.13834846549046	21.368241038029645	36.13460312678079
10-11	24.132708421316128	26.958930188726676	29.08371290296687	19.824648486990323
12-13	24.914081583755404	24.379175593777973	29.31077985118685	21.395962971279772
14-15	21.968891207163338	25.846119486168746	26.769349961495465	25.41563934517245
16-17	21.68100725712579	29.04381477258361	27.39377532878126	21.881402641509343
18-19	21.693096195058352	28.288787161322375	28.999150803562685	21.018965840056584
20-21	22.43999490212557	27.446855969419744	28.856269740067614	21.256879388387077
22-23	22.174002119814205	27.380632555393458	28.73262689362473	21.712738431167608
24-25	21.94075114355936	27.46774192138945	28.815919057037714	21.775587878013475
26-27	21.914218603202094	27.647161240362404	28.695197977523236	21.743422178912265
28-29	21.834717158876224	27.775542761477713	28.664769294964838	21.72497078468123
30-31	22.13938382958909	27.610651562617633	28.575115353774365	21.67484925401891
32-33	21.925862402813827	27.744160633493326	28.538856882227876	21.791120081464975
34-35	22.022127270587447	27.752031872821902	28.564516748621788	21.661324107968866
36-37	22.036968051392865	27.69242845481913	28.497099618330346	21.77350387545766
38-39	21.937026875406275	27.763794633625572	28.583458411538132	21.71572007943002
40-41	22.109816197919343	27.693785690767648	28.45090799709907	21.745490114213943
42-43	21.985091964090266	27.814952257937254	28.290587633209064	21.909368144763413
44-45	22.10637743164142	27.846342077052938	28.13629834874259	21.910982142563057
46-47	22.12092289950423	27.85350255113233	27.92688059457666	22.09869395478678
48-49	22.114291967761734	27.94922839464671	27.868977176319742	22.06750246127182
50-51	22.09415862472323	28.05204808455529	27.766747086101017	22.08704620462046
52-53	22.222602185203993	27.969623663293596	27.678019345829853	22.129754805672558
54-55	22.1986345961675	27.911696690114578	27.688880819189134	22.200787894528787
56-57	22.20521986730898	27.961176723535143	27.71959284895411	22.114010560201766
58-59	22.213334621737165	28.031622071680694	27.622195966113892	22.13284734046825
60-61	22.23668469268352	27.984398280550366	27.68203580943232	22.09688121733379
62-63	22.26256210914721	28.030825956833194	27.588255314430437	22.11835661958916
64-65	22.222738180494943	28.03481813085566	27.560956092658806	22.18148759599059
66-67	22.269658552714677	27.994627826631657	27.486703808803387	22.249009811850275
68-69	22.210194696352037	28.02400905257488	27.56111612649172	22.20468012458137
70-71	22.19584537521982	27.829026065558637	27.605364979082875	22.369763580138667
72-73	22.267214918184486	27.883094466052178	27.552149863927934	22.297540751835403
74-75	22.108131838025137	28.00490195933712	27.579242782496667	22.307723420141077
76-77	22.268087629094985	27.93966220215418	27.473435776514183	22.31881439223665
78-79	22.224199338769278	27.97454013467063	27.471559269071687	22.329701257488406
80-81	22.2262196540557	27.9733805280299	27.494540641989317	22.30585917592508
82-83	22.214040252238014	27.932139371074104	27.599481824160232	22.25433855252765
84-85	22.32438605078811	27.91234391559926	27.50358971401997	22.25968031959266
86-87	22.186806835022356	27.921402421409784	27.59285269325611	22.29893805031175
88-89	22.299819062195215	27.8624859820153	27.501343591192217	22.336351364597267
90-91	22.292064653950266	27.928292530666717	27.518824127970838	22.26081868741218
92-93	22.26092939484517	27.910311431610186	27.55526146210576	22.273497711438882
94-95	22.183510055606472	27.94348438765751	27.595142321777384	22.277863234958637
96-97	22.203956966019607	27.973890156882565	27.59903149872448	22.22312137837335
98-99	22.406945538606713	28.523006611891926	27.700186213052724	21.369861636448636
100-101	23.204469967416635	31.071759411750378	24.833662686907267	20.890107933925716
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2483.0
1	2100.5
2	2631.5
3	3783.5
4	5157.5
5	8195.0
6	10728.5
7	12520.5
8	13737.0
9	13478.0
10	12070.5
11	10688.5
12	11466.5
13	15178.0
14	23674.5
15	35013.0
16	44197.5
17	49495.5
18	50877.0
19	49220.0
20	46488.5
21	44566.0
22	45419.0
23	51018.0
24	61823.0
25	77378.0
26	97229.5
27	119597.5
28	144364.5
29	170355.5
30	193276.0
31	215524.5
32	238827.0
33	259472.0
34	278105.0
35	296955.0
36	317307.5
37	336463.0
38	352074.0
39	364060.5
40	375741.5
41	388333.5
42	398851.5
43	408827.0
44	417481.5
45	425027.5
46	430283.5
47	432422.5
48	431615.5
49	425466.0
50	417660.0
51	405592.0
52	387896.0
53	369403.5
54	348237.0
55	323320.5
56	297747.5
57	270844.5
58	242091.5
59	211532.0
60	177190.5
61	144621.0
62	116233.0
63	90206.0
64	67578.5
65	50282.5
66	37764.5
67	28653.0
68	21713.5
69	15627.5
70	11161.5
71	8229.5
72	5951.0
73	4186.5
74	2919.0
75	1993.0
76	1396.5
77	978.5
78	662.5
79	456.5
80	333.0
81	242.0
82	172.5
83	118.5
84	79.0
85	58.0
86	50.5
87	40.0
88	27.0
89	16.0
90	11.5
91	10.0
92	7.5
93	4.5
94	2.0
95	1.0
96	1.0
97	1.5
98	2.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004367794715977839
3	5.823726287970453E-5
4	4.853105239975378E-5
5	9.706210479950756E-5
6	0.0
7	1.941242095990151E-5
8	4.076608401579317E-4
9	8.735589431955678E-5
10-11	4.853105239975378E-5
12-13	2.96039419638498E-4
14-15	0.0
16-17	4.8045741875756237E-4
18-19	9.706210479950755E-6
20-21	1.9413074802945158E-5
22-23	0.0
24-25	0.0
26-27	8.741847559370866E-5
28-29	1.4579712805901208E-5
30-31	7.786522745771248E-5
32-33	4.0492296560115985E-4
34-35	0.0
36-37	3.63722960085583E-4
38-39	0.0011378111975462854
40-41	0.0
42-43	0.0
44-45	2.8883556297496985E-4
46-47	0.0
48-49	0.0
50-51	5.222038464490921E-5
52-53	6.112156061469954E-4
54-55	0.0030889572913640625
56-57	0.0035269217939180404
58-59	0.004104720808494441
60-61	9.202918267294269E-4
62-63	1.1066339774367291E-5
64-65	5.643843837634995E-4
66-67	0.0
68-69	0.0
70-71	5.773984757373119E-6
72-73	9.927569040256934E-5
74-75	1.771365382607837E-5
76-77	0.0
78-79	0.0
80-81	4.6468910067434333E-4
82-83	0.0
84-85	0.0
86-87	6.34621134038774E-6
88-89	0.0
90-91	0.0011686089949205331
92-93	1.6580865111279824E-4
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	196.0
20-21	776.0
22-23	1589.0
24-25	3628.0
26-27	5885.0
28-29	10787.0
30-31	24296.0
32-33	29119.0
34-35	39581.0
36-37	61027.0
38-39	74228.0
40-41	75179.0
42-43	85126.0
44-45	99153.0
46-47	100097.0
48-49	95128.0
50-51	86952.0
52-53	83616.0
54-55	89290.0
56-57	92711.0
58-59	93264.0
60-61	92581.0
62-63	88279.0
64-65	91345.0
66-67	95826.0
68-69	98606.0
70-71	98723.0
72-73	94179.0
74-75	95639.0
76-77	92976.0
78-79	99755.0
80-81	102099.0
82-83	98846.0
84-85	98340.0
86-87	102608.0
88-89	110851.0
90-91	122050.0
92-93	120622.0
94-95	138316.0
96-97	215441.0
98-99	877057.0
100-101	6216915.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.70134620188881
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.93749872871429	74.44188371422162
2	7.473181993770544	12.5103078658062
3	1.9848997662712875	4.984163475381638
4	0.7355200019351542	2.4625605728155313
5	0.3458432903776531	1.4473774489750153
6	0.17127003625517248	0.8601319559162544
7	0.10705307696258257	0.6272340659775799
8	0.06490282960225806	0.43459633680166404
9	0.04323844004904024	0.32572040757968984
>10	0.1340068353887782	1.7242812625161457
>50	0.002119714106724913	0.11667750248727453
>100	4.652865665985697E-4	0.06506539152144501
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.706210479950755E-6
2	0.0	0.0	0.0	9.706210479950755E-6	9.706210479950755E-6
3	0.0	0.0	0.0	9.706210479950755E-6	9.706210479950755E-6
4	0.0	1.941242095990151E-5	0.0	9.706210479950755E-6	9.706210479950755E-6
5	0.0	1.941242095990151E-5	0.0	9.706210479950755E-6	9.706210479950755E-6
6	0.0	2.9118631439852264E-5	0.0	9.706210479950755E-6	1.941242095990151E-5
7	0.0	2.9118631439852264E-5	0.0	9.706210479950755E-6	1.941242095990151E-5
8	0.0	2.9118631439852264E-5	0.0	9.706210479950755E-6	1.941242095990151E-5
9	0.0	9.706210479950755E-5	0.0	9.706210479950755E-6	2.9118631439852264E-5
10-11	0.0	9.706210479950755E-5	0.0	9.706210479950755E-6	2.9118631439852264E-5
12-13	0.0	1.0191521003948292E-4	0.0	9.706210479950755E-6	2.9118631439852264E-5
14-15	0.0	2.135366305589166E-4	0.0	1.4559315719926132E-5	3.882484191980302E-5
16-17	0.0	2.135366305589166E-4	0.0	2.9118631439852264E-5	3.882484191980302E-5
18-19	0.0	2.523614724787196E-4	0.0	4.853105239975377E-5	4.367794715977839E-5
20-21	0.0	2.9118631439852265E-4	0.0	6.794347335965529E-5	4.853105239975377E-5
22-23	0.0	3.203049458383749E-4	0.0	8.250278907958141E-5	9.706210479950755E-5
24-25	0.0	3.7854220871807944E-4	0.0	1.261807362393598E-4	1.5529936767921207E-4
26-27	0.0	4.076608401579317E-4	0.0	2.9118631439852265E-4	1.6500557815916282E-4
28-29	0.0	4.076608401579317E-4	0.0	0.0014171067300728102	1.6500557815916282E-4
30-31	0.0	4.5133878731771007E-4	0.0	0.004950167344774885	1.698586833991382E-4
32-33	0.0	4.5619189255768547E-4	0.0	0.011720249154540535	1.7471178863911357E-4
34-35	0.0	5.095760501974147E-4	0.0	0.019960821852018727	1.8441799911906434E-4
36-37	0.0	5.629602078371438E-4	0.0	0.03409306431082702	1.8441799911906434E-4
38-39	0.0	5.7751952355707E-4	0.0	0.05217573443497528	1.8441799911906434E-4
40-41	0.0	5.92078839276996E-4	0.0	0.06561883594970708	1.8927110435903972E-4
42-43	0.0	6.16344365476873E-4	0.0	0.07610154326805389	1.9897731483899047E-4
44-45	0.0	7.182595755163559E-4	0.0	0.0870598548999183	2.0383042007896584E-4
46-47	0.0	7.862030488760112E-4	0.0	0.09995455552253288	2.0383042007896584E-4
48-49	0.0	7.862030488760112E-4	0.0	0.11040814420943984	2.0383042007896584E-4
50-51	0.0	8.638527327156172E-4	0.0	0.11969213453351273	2.0383042007896584E-4
52-53	0.0	8.638527327156172E-4	0.0	0.1278356451261914	2.1838973579889196E-4
54-55	0.0	8.784120484355433E-4	0.0	0.136197545454669	2.329490515188181E-4
56-57	0.0	8.929713641554694E-4	0.0	0.14426340636350807	2.329490515188181E-4
58-59	0.0	9.075306798753955E-4	0.0	0.1515915952758709	2.3780215675879348E-4
60-61	0.0	9.269431008352971E-4	0.0	0.15883242829391414	2.475083672387442E-4
62-63	0.0	9.366493113152478E-4	0.0	0.16602473025955766	2.523614724787196E-4
64-65	0.0	0.0010191521003948293	0.0	0.172658925122604	2.523614724787196E-4
66-67	9.706210479950755E-6	0.0010337114161147554	0.0	0.17933679793281013	2.523614724787196E-4
68-69	9.706210479950755E-6	0.0010579769423146321	0.0	0.18631556326789472	2.523614724787196E-4
70-71	9.706210479950755E-6	0.001082242468514509	0.0	0.19302255470954066	2.6206768295867035E-4
72-73	9.706210479950755E-6	0.0010919486789944598	0.0	0.19828332078967398	3.008925248784734E-4
74-75	9.706210479950755E-6	0.0011210673104343123	0.0	0.2025540534008523	3.008925248784734E-4
76-77	9.706210479950755E-6	0.0011501859418741643	0.0	0.2058881367007154	3.008925248784734E-4
78-79	9.706210479950755E-6	0.0011550390471141397	0.0	0.20907177373813926	3.008925248784734E-4
80-81	9.706210479950755E-6	0.0011550390471141397	0.0	0.21059564878349152	3.1059873535842415E-4
82-83	9.706210479950755E-6	0.0011550390471141397	0.0	0.21117802141228859	3.203049458383749E-4
84-85	9.706210479950755E-6	0.0011550390471141397	0.0	0.2117603940410856	3.251580510783503E-4
86-87	9.706210479950755E-6	0.0011647452575940906	0.0	0.21208555209216395	3.3001115631832564E-4
88-89	9.706210479950755E-6	0.001184157678553992	0.0	0.21218746730220345	3.3001115631832564E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11395	0.0	44.824173	1
GTATCAA	20030	0.0	34.809628	1
TCAACGC	23515	0.0	28.768957	4
ATCAACG	23705	0.0	28.538368	3
CAACGCA	24100	0.0	28.08817	5
AACGCAG	24670	0.0	27.473734	6
TATCAAC	25155	0.0	27.39839	2
ACGCAGA	28905	0.0	23.41918	7
CGCAGAG	29275	0.0	23.123192	8
GTACATG	28585	0.0	21.388964	1
TACATGG	28570	0.0	20.91191	2
ACATGGG	28490	0.0	20.257662	3
GCAGAGT	35180	0.0	19.181751	9
CATGGGG	20635	0.0	18.543564	4
GAGTACT	19985	0.0	17.983377	12-13
AGAGTAC	31405	0.0	17.596655	10-11
GTGGTAT	6525	0.0	17.496168	1
CAGAGTA	34385	0.0	17.07995	10-11
TGGTATC	6290	0.0	15.998824	2
GTACTTT	23340	0.0	15.44414	14-15
>>END_MODULE