FastQCFastQC Report
Fri 27 May 2016
SRR2138794_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138794_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11174888
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119700.044.1323131
GTATCAA209950.036.0539671
ATCAACG247800.029.7792433
TCAACGC249400.029.6389484
CAACGCA253350.029.0269625
AACGCAG264350.027.9471686
TATCAAC268150.027.85122
ACGCAGA303250.024.2647937
CGCAGAG306300.023.9818528
GTACATG319050.021.4505561
TACATGG317000.021.3098562
ACATGGG319450.020.524533
GCAGAGT370900.019.7479449
CATGGGG226550.018.679364
GAGTACT201400.018.21537812-13
AGAGTAC324300.018.19472710-11
CAGAGTA354550.017.58247410-11
CTTATAC127800.016.0784661
GTGGTAT66650.015.8898591
TATACTG58500.015.7947715
Produced by FastQC (version 0.11.5)