##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138794_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11174888
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3879620091047	33.0	31.0	34.0	31.0	34.0
2	32.5486105990503	34.0	31.0	34.0	31.0	34.0
3	32.66603208909118	34.0	31.0	34.0	31.0	34.0
4	36.172631260375944	37.0	35.0	37.0	35.0	37.0
5	36.016004366218255	37.0	35.0	37.0	35.0	37.0
6	36.05972829436859	37.0	35.0	37.0	35.0	37.0
7	36.035718747248296	37.0	35.0	37.0	35.0	37.0
8	36.022742867758495	37.0	35.0	37.0	35.0	37.0
9	37.86151673287464	39.0	38.0	39.0	35.0	39.0
10-11	37.74468612123898	39.0	37.5	39.0	35.0	39.0
12-13	37.762292114247586	39.0	37.5	39.0	35.0	39.0
14-15	39.09443454824782	40.0	38.5	41.0	36.0	41.0
16-17	39.046046054331825	40.0	38.0	41.0	36.0	41.0
18-19	39.10745897408547	40.0	38.5	41.0	36.0	41.0
20-21	39.10680749775746	40.0	39.0	41.0	36.0	41.0
22-23	39.069879527331295	40.0	39.0	41.0	36.0	41.0
24-25	39.05299066302034	40.0	39.0	41.0	36.0	41.0
26-27	38.9579587254802	40.0	38.5	41.0	35.5	41.0
28-29	38.88117900628288	40.0	38.0	41.0	35.0	41.0
30-31	38.74417768865974	40.0	38.0	41.0	35.0	41.0
32-33	38.655657638398836	40.0	38.0	41.0	35.0	41.0
34-35	38.60774705878891	40.0	38.0	41.0	35.0	41.0
36-37	38.51083860332605	40.0	38.0	41.0	34.5	41.0
38-39	38.46758998803581	40.0	38.0	41.0	34.0	41.0
40-41	38.46858214349004	40.0	38.0	41.0	34.0	41.0
42-43	38.43185921545133	40.0	38.0	41.0	34.0	41.0
44-45	38.32236299130708	40.0	38.0	41.0	34.0	41.0
46-47	38.26197554043176	40.0	38.0	41.0	34.0	41.0
48-49	38.232293697926515	40.0	38.0	41.0	34.0	41.0
50-51	38.146943435668454	40.0	37.5	41.0	33.0	41.0
52-53	38.01677634000626	40.0	37.0	41.0	33.0	41.0
54-55	37.837563138536254	40.0	37.0	41.0	33.0	41.0
56-57	37.70569749116932	39.0	36.5	41.0	33.0	41.0
58-59	37.479243966066235	39.0	36.0	41.0	32.5	41.0
60-61	37.30483747433432	39.0	36.0	40.5	32.0	41.0
62-63	37.08793000372037	39.0	35.0	40.0	32.0	41.0
64-65	36.77406958984861	38.0	35.0	40.0	31.0	41.0
66-67	36.69888192957478	38.0	35.0	40.0	31.0	41.0
68-69	36.4630645571368	37.0	35.0	40.0	31.0	41.0
70-71	36.152427664470245	37.0	35.0	39.5	31.0	41.0
72-73	35.837712377026946	36.5	34.5	39.0	31.0	41.0
74-75	35.440594645398534	36.0	34.0	39.0	30.5	40.0
76-77	33.40150424976632	34.5	32.0	36.5	27.5	39.0
78-79	34.59388894647117	35.0	34.0	37.0	30.0	39.0
80-81	34.60243105604437	35.0	34.0	37.0	30.0	39.0
82-83	34.319924171120995	35.0	34.0	36.0	30.0	38.5
84-85	34.06643012328979	35.0	34.0	36.0	30.0	37.0
86-87	33.85999384797401	35.0	34.0	36.0	30.0	37.0
88-89	33.629608182612074	35.0	33.5	35.0	29.5	36.5
90-91	33.471918358031196	35.0	33.0	35.0	29.5	36.0
92-93	33.41168755398979	35.0	33.0	35.0	29.0	36.0
94-95	33.38764436870332	35.0	33.0	35.0	30.0	36.0
96-97	33.42435325140504	35.0	33.0	35.0	30.0	35.5
98-99	33.45478904607096	35.0	33.0	35.0	30.0	35.0
100-101	32.168542160779474	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	9.0
20	48.0
21	219.0
22	917.0
23	3194.0
24	8686.0
25	19239.0
26	35444.0
27	57801.0
28	86134.0
29	119015.0
30	158089.0
31	206297.0
32	269885.0
33	367311.0
34	534106.0
35	823169.0
36	1471002.0
37	2686890.0
38	3084779.0
39	1223537.0
40	19115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.478599159114616	20.338253054527257	14.021661783097962	24.161486003260165
2	14.387719461055632	21.10262571566719	42.08877051873113	22.42088430454604
3	18.239492189534417	26.178827187196134	30.344501300148423	25.237179323121023
4	12.310364921908809	18.192600478000486	38.96259683769424	30.53443776239646
5	13.02326522043462	37.99757813910809	35.83509368066479	13.14406295979249
6	29.743832779353134	38.24194032190748	18.93611819644188	13.07810870229751
7	26.38848396305788	32.503785720946055	23.293553061749357	17.81417725424671
8	24.047058541383162	37.40991711116939	21.320077133941968	17.222947213505474
9	25.418431973512007	16.84511012427432	21.2609268560051	36.47553104620857
10-11	23.997093302250523	26.91031908573877	29.140383201413204	19.952204410597503
12-13	24.803910312620445	24.326955834171002	29.228645439079738	21.640488414128818
14-15	21.917235322626947	25.818871741712314	26.661528956710796	25.602363978949942
16-17	21.673055571886884	28.997687752335803	27.333068989527458	21.996187686249854
18-19	21.66651789559403	28.251581951048728	28.853171899940826	21.22872825341642
20-21	22.425125957723235	27.422699223637498	28.663517199698763	21.48865761894051
22-23	22.049851413056043	27.474217953502077	28.587323429804357	21.888607203637527
24-25	21.855926189739368	27.549449181100954	28.76687876810069	21.827745861058983
26-27	21.83370717290642	27.71265926987441	28.63372624734396	21.81990730987521
28-29	21.65620081495146	27.716679244626985	28.668694105722704	21.958425834698854
30-31	22.01317858421509	27.552399995889854	28.595861252753878	21.83856016714118
32-33	21.7776911684535	27.794638555943063	28.56132450727939	21.866345768324045
34-35	21.876751070650595	27.883829609817518	28.63633223203771	21.60308708749418
36-37	21.927614311945103	27.747757933320255	28.444572020850163	21.880055733884483
38-39	21.8227180622542	27.766873446855406	28.607455130567054	21.80295336032334
40-41	22.01984192452721	27.628965821013747	28.551778361710213	21.799413892748838
42-43	21.993917766569567	27.686388099084542	28.42071658203369	21.898977552312207
44-45	21.94488031634497	27.91328378279438	28.230295460213245	21.9115404406474
46-47	22.12442245888168	27.749017294826768	27.92405903313216	22.202501213159394
48-49	22.034999834827175	27.844719978598185	27.960130908532637	22.160149278042002
50-51	21.956620473986845	28.020241400225448	27.921054585533923	22.10208354025378
52-53	22.08305780887838	27.91372371842144	27.878717050125513	22.124501422574664
54-55	22.06205478770866	27.844640021135596	27.83501374309501	22.25829144806073
56-57	22.203585079336598	27.96630047233666	27.688688060029236	22.14142638829751
58-59	22.015322869382555	28.11098564607677	27.843947091033368	22.029744393507308
60-61	22.19353824672881	28.157819789807604	27.665211096179586	21.983430867283992
62-63	22.04991291689053	28.207111037857658	27.626930403645446	22.116045641606362
64-65	22.04164421851769	28.141826901075756	27.6304512159386	22.186077664467952
66-67	22.107692144336248	28.13690438424207	27.502242755980568	22.25316071544112
68-69	22.07391876484108	28.101776324356255	27.58478537271687	22.239519538085794
70-71	22.08118511016532	27.824644369058042	27.67034609125667	22.423824429519964
72-73	22.136525389862474	27.861976769438762	27.616022117554547	22.385475723144218
74-75	21.999650377635433	27.961944301355413	27.615006282739092	22.423399038270063
76-77	22.132599115110494	27.939640098833774	27.503002186929653	22.424758599126083
78-79	22.154881935956375	27.927481678944233	27.47816444943337	22.439471935666024
80-81	22.124954948917136	27.9616117956631	27.49295001272014	22.420483242699625
82-83	22.167124589751296	27.887435398939026	27.59182071406118	22.353619297248496
84-85	22.31701588685296	27.890039012889762	27.476726249685985	22.31621885057129
86-87	22.080854827138165	27.946363357731425	27.576501184069098	22.39628063106132
88-89	22.20635162482704	27.889753585690958	27.51554209380757	22.388352695674435
90-91	22.17980816032867	27.902203380985867	27.59286080487132	22.325127653814143
92-93	22.159559438554595	27.88335215064092	27.620482491529746	22.336605919274735
94-95	22.045513113814337	27.953003040568714	27.659314305548982	22.342169540067964
96-97	22.004826642761348	27.97450326280795	27.72915850671826	22.291511587712442
98-99	22.239428479655373	28.579403963183026	27.78896206733583	21.392205489825773
100-101	23.069443211419404	31.101269674337225	24.84823927818335	20.981047836060025
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2402.0
1	2147.5
2	2826.0
3	4028.5
4	5639.5
5	9124.0
6	11993.5
7	14021.0
8	15225.0
9	14763.5
10	13338.5
11	12008.5
12	12851.5
13	16828.0
14	26260.0
15	38590.5
16	47889.5
17	52933.0
18	53657.0
19	51216.0
20	48087.0
21	45941.0
22	46849.0
23	52996.0
24	65068.0
25	82393.0
26	104263.0
27	128066.0
28	152390.0
29	178113.5
30	202206.0
31	226018.5
32	251490.5
33	276149.0
34	299212.5
35	321529.5
36	346099.5
37	369476.0
38	387395.0
39	401236.0
40	415428.0
41	427774.5
42	437081.5
43	447891.5
44	459549.5
45	470695.5
46	480364.0
47	489082.0
48	489326.0
49	481534.5
50	472298.0
51	456785.5
52	432293.0
53	405294.0
54	378707.5
55	348291.5
56	317679.0
57	285152.0
58	251636.0
59	219539.0
60	184197.0
61	149962.5
62	119364.5
63	92393.5
64	69463.0
65	51566.0
66	39132.0
67	29904.0
68	22521.0
69	16060.0
70	11175.5
71	8010.0
72	5596.5
73	3798.5
74	2592.5
75	1762.0
76	1232.0
77	840.0
78	581.5
79	420.5
80	294.0
81	210.0
82	157.0
83	112.5
84	86.5
85	71.0
86	58.0
87	45.0
88	29.5
89	17.0
90	12.0
91	8.5
92	5.5
93	4.5
94	3.5
95	3.0
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004698033662619259
3	8.948635547846206E-5
4	3.5794542191384824E-5
5	8.948635547846206E-5
6	0.0
7	1.7897271095692412E-5
8	3.6689405746169446E-4
9	1.1633226212200068E-4
10-11	6.711476660884655E-5
12-13	2.4161315979184757E-4
14-15	0.0
16-17	4.2058587074877176E-4
18-19	4.474317773923103E-6
20-21	4.921925288932675E-5
22-23	0.0
24-25	0.0
26-27	8.507371010121354E-5
28-29	1.3441979175506633E-5
30-31	9.422816587567357E-5
32-33	3.958582253558799E-4
34-35	0.0
36-37	4.1252623678199074E-4
38-39	0.0011043650530706735
40-41	0.0
42-43	0.0
44-45	2.9454578351866727E-4
46-47	4.7246006649402975E-6
48-49	7.638043797688842E-5
50-51	3.4232158934031784E-4
52-53	6.373654368303223E-4
54-55	0.003519530053890534
56-57	0.0037521821020715467
58-59	0.004621684573482857
60-61	9.968868287010904E-4
62-63	2.0461853707874605E-5
64-65	5.791369132922935E-4
66-67	0.0
68-69	0.0
70-71	1.6065482908334373E-5
72-73	1.245941886020153E-4
74-75	5.4782540431843094E-5
76-77	5.540878998395251E-6
78-79	0.0
80-81	4.5965938445139136E-4
82-83	0.0
84-85	0.0
86-87	1.7672230316758235E-5
88-89	0.0
90-91	0.001109111746947973
92-93	1.7856706335442416E-4
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	253.0
20-21	826.0
22-23	1828.0
24-25	3930.0
26-27	6395.0
28-29	12125.0
30-31	27040.0
32-33	32785.0
34-35	44202.0
36-37	68119.0
38-39	80277.0
40-41	82658.0
42-43	93311.0
44-45	110140.0
46-47	110163.0
48-49	105704.0
50-51	96554.0
52-53	88871.0
54-55	96527.0
56-57	102067.0
58-59	102694.0
60-61	109000.0
62-63	103813.0
64-65	107653.0
66-67	114970.0
68-69	109005.0
70-71	108355.0
72-73	103166.0
74-75	103948.0
76-77	101264.0
78-79	108573.0
80-81	111200.0
82-83	107020.0
84-85	105178.0
86-87	111343.0
88-89	119649.0
90-91	132254.0
92-93	130036.0
94-95	149864.0
96-97	230950.0
98-99	945911.0
100-101	6695267.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.55982484435545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.03420721412668	74.39682759968474
2	7.425657684902418	12.409733110091759
3	1.8820455117350838	4.717901799290668
4	0.7564031554960232	2.528196607398618
5	0.34154503877396075	1.4269721808205373
6	0.18888806698538377	0.9470072275492527
7	0.10734973216691936	0.6279087371869375
8	0.06989514507206879	0.46723408637543046
9	0.045341232658226184	0.34098349132336936
>10	0.14482316234927148	1.8239283060753058
>50	0.003166038444581022	0.17372830225025174
>100	6.040678458429294E-4	0.08101745857469776
>500	3.69780465477005E-5	0.017565771304063685
>1k	3.697139695454439E-5	0.04099532207440796
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.7897271095692412E-5	0.0
2	0.0	1.7897271095692412E-5	0.0	1.7897271095692412E-5	0.0
3	0.0	1.7897271095692412E-5	0.0	1.7897271095692412E-5	0.0
4	0.0	8.053771993061586E-5	0.0	1.7897271095692412E-5	0.0
5	0.0	8.948635547846207E-5	0.0	1.7897271095692412E-5	0.0
6	0.0	8.948635547846207E-5	0.0	1.7897271095692412E-5	0.0
7	0.0	8.948635547846207E-5	0.0	1.7897271095692412E-5	0.0
8	0.0	1.0738362657415448E-4	0.0	2.684590664353862E-5	0.0
9	0.0	2.4161315979184757E-4	0.0	8.053771993061586E-5	0.0
10-11	0.0	2.4161315979184757E-4	0.0	8.053771993061586E-5	0.0
12-13	0.0	2.4608747756577065E-4	0.0	8.053771993061586E-5	0.0
14-15	0.0	3.847913285573869E-4	0.0	8.501203770453896E-5	0.0
16-17	0.0	3.982142818791562E-4	0.0	8.948635547846207E-5	0.0
18-19	0.0	4.2506018852269485E-4	0.0	1.0290930880023138E-4	0.0
20-21	0.0	4.4743177739231033E-4	0.0	1.1185794434807758E-4	0.0
22-23	0.0	5.100722262272339E-4	0.0	1.6554975763515483E-4	0.0
24-25	0.0	5.995585817056958E-4	0.0	2.2819020647007825E-4	0.0
26-27	0.0	6.443017594449269E-4	0.0	3.5347110413992515E-4	0.0
28-29	0.0	6.4877607721885E-4	0.0	0.0012975521544377	0.0
30-31	0.0	7.38262432697312E-4	0.0	0.003847913285573869	0.0
32-33	0.0	7.472110682451582E-4	0.0	0.008774137154663205	0.0
34-35	0.0	8.63543330367159E-4	0.0	0.014917375458259625	0.0
36-37	0.0	9.977728635848521E-4	0.0	0.024465569587811528	0.0
38-39	0.0	0.0010514646768719294	0.0	0.04218834229032094	0.0
40-41	0.0	0.0011096308079329297	0.0	0.07879273599878585	0.0
42-43	0.0	0.0011498996678982376	0.0	0.09393382734574163	0.0
44-45	0.0	0.0012707062477941614	0.0	0.1141085261883609	0.0
46-47	0.0	0.0013333466966290847	0.0	0.13848460942069396	0.0
48-49	0.0	0.0013512439677247773	0.0	0.15891434437642687	0.0
50-51	0.0	0.0015346909964556244	0.0	0.1799525865494133	0.0
52-53	0.0	0.0015525882675513168	0.0	0.2073980517746576	0.0
54-55	0.0	0.0015704855386470093	0.0	0.25956412270082707	0.0
56-57	0.0	0.0015839084919687786	0.0	0.31831638939021134	0.0
58-59	0.0	0.001597331445290548	0.0	0.37932818655542677	0.0
60-61	0.0	0.0016241773519340866	0.0	0.4184605698061582	0.0
62-63	0.0	0.001646548940803702	0.0	0.4373958826254008	0.0
64-65	0.0	0.0018255216517606262	0.0	0.454783081494866	0.0
66-67	0.0	0.001861316193952011	0.0	0.4623312555794743	0.0
68-69	0.0	0.001950802549430473	0.0	0.46898904042707185	0.0
70-71	0.0	0.0019955457271697043	0.0	0.47552154437699956	0.0
72-73	0.0	0.0020134429982653964	0.0	0.48057304914375876	0.0
74-75	0.0	0.002080557764874243	0.0	0.4847654848979247	0.0
76-77	0.0	0.002165569802578782	0.0	0.4882509784438108	0.0
78-79	0.0	0.0022013643447701668	0.0	0.4915843451853835	0.0
80-81	0.0	0.0022013643447701668	0.0	0.4929042689286908	0.0
82-83	0.0	0.0022103129803180133	0.0	0.49346803296820513	0.0
84-85	0.0	0.002232684569187629	0.0	0.4940228483721716	0.0
86-87	0.0	0.0022729534291529366	0.0	0.4942599872141895	0.0
88-89	0.0	0.002389285691274937	0.0	0.49443895992514647	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11970	0.0	44.132313	1
GTATCAA	20995	0.0	36.053967	1
ATCAACG	24780	0.0	29.779243	3
TCAACGC	24940	0.0	29.638948	4
CAACGCA	25335	0.0	29.026962	5
AACGCAG	26435	0.0	27.947168	6
TATCAAC	26815	0.0	27.8512	2
ACGCAGA	30325	0.0	24.264793	7
CGCAGAG	30630	0.0	23.981852	8
GTACATG	31905	0.0	21.450556	1
TACATGG	31700	0.0	21.309856	2
ACATGGG	31945	0.0	20.52453	3
GCAGAGT	37090	0.0	19.747944	9
CATGGGG	22655	0.0	18.67936	4
GAGTACT	20140	0.0	18.215378	12-13
AGAGTAC	32430	0.0	18.194727	10-11
CAGAGTA	35455	0.0	17.582474	10-11
CTTATAC	12780	0.0	16.078466	1
GTGGTAT	6665	0.0	15.889859	1
TATACTG	5850	0.0	15.794771	5
>>END_MODULE