FastQCFastQC Report
Fri 27 May 2016
SRR2138793_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138793_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8879360
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108050.041.060821
CTTATAC81400.036.199241
GTATCAA181000.031.5908371
TATACAC117450.028.9605373
ATCAACG205800.027.1542133
TCAACGC206400.027.0752774
CAACGCA206100.027.0741885
AACGCAG211900.026.5497826
TTATACA121750.026.1219232
TATCAAC223100.025.3301812
ACGCAGA243850.022.9342257
CGCAGAG247450.022.6005698
ACACATC140150.021.5897166
ACATCTC143800.021.5351098
GTACATG252650.021.2442231
TACACAT161500.021.0355535
TACATGG250600.020.8517442
ACATGGG251100.020.2276733
CACATCT162400.018.503277
GCAGAGT302200.018.478379
Produced by FastQC (version 0.11.5)