FastQCFastQC Report
Fri 27 May 2016
SRR2138792_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138792_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11252341
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122500.044.9370271
GTATCAA212950.034.697241
TCAACGC248800.028.8182144
ATCAACG250150.028.6963143
CAACGCA255300.028.06795
AACGCAG263250.027.3163556
TATCAAC266750.027.1320782
ACGCAGA310250.023.056197
CGCAGAG315950.022.6934788
GTACATG311600.021.6879861
TACATGG302000.021.500422
ACATGGG302650.020.85613
GCAGAGT383750.018.68389
GTGGTAT68900.018.5547541
CATGGGG216650.018.1098984
GAGTACT217150.017.66136212-13
AGAGTAC334350.017.23095710-11
CAGAGTA366750.016.97572910-11
TGGTATC68750.016.5157242
GTACTTT246050.015.63883114-15
Produced by FastQC (version 0.11.5)