##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138792_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11252341
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37743897025517	33.0	31.0	34.0	31.0	34.0
2	32.54486377545793	34.0	31.0	34.0	31.0	34.0
3	32.66142325405887	34.0	31.0	34.0	31.0	34.0
4	36.16947753360834	37.0	35.0	37.0	35.0	37.0
5	36.01068622076064	37.0	35.0	37.0	35.0	37.0
6	36.05568619010035	37.0	35.0	37.0	35.0	37.0
7	36.03214975443777	37.0	35.0	37.0	35.0	37.0
8	36.01779194213897	37.0	35.0	37.0	35.0	37.0
9	37.85073132781881	39.0	38.0	39.0	35.0	39.0
10-11	37.73509761213245	39.0	37.0	39.0	35.0	39.0
12-13	37.75204306375002	39.0	37.0	39.0	35.0	39.0
14-15	39.08326525120417	40.0	38.5	41.0	36.0	41.0
16-17	39.03633159535425	40.0	38.0	41.0	36.0	41.0
18-19	39.09738951210241	40.0	38.5	41.0	36.0	41.0
20-21	39.0968976662824	40.0	39.0	41.0	36.0	41.0
22-23	39.0628378668998	40.0	39.0	41.0	36.0	41.0
24-25	39.04792951444027	40.0	39.0	41.0	36.0	41.0
26-27	38.95283229374168	40.0	38.0	41.0	35.5	41.0
28-29	38.871439254978974	40.0	38.0	41.0	35.0	41.0
30-31	38.73197583122713	40.0	38.0	41.0	35.0	41.0
32-33	38.64457814854607	40.0	38.0	41.0	35.0	41.0
34-35	38.60095420751986	40.0	38.0	41.0	35.0	41.0
36-37	38.50339370536348	40.0	38.0	41.0	34.5	41.0
38-39	38.46862220592905	40.0	38.0	41.0	34.0	41.0
40-41	38.465962186070094	40.0	38.0	41.0	34.0	41.0
42-43	38.428181929034565	40.0	38.0	41.0	34.0	41.0
44-45	38.321984749706345	40.0	38.0	41.0	34.0	41.0
46-47	38.26422531865538	40.0	38.0	41.0	34.0	41.0
48-49	38.238563552243164	40.0	38.0	41.0	34.0	41.0
50-51	38.162261664815546	40.0	37.5	41.0	33.0	41.0
52-53	38.0331251884173	40.0	37.0	41.0	33.0	41.0
54-55	37.8617275214879	40.0	37.0	41.0	33.0	41.0
56-57	37.73989930794599	39.0	37.0	41.0	33.0	41.0
58-59	37.518338978207595	39.0	36.0	41.0	32.5	41.0
60-61	37.35535018257696	39.0	36.0	40.5	32.0	41.0
62-63	37.137820579007865	39.0	35.0	40.0	32.0	41.0
64-65	36.81719551428202	38.5	35.0	40.0	31.0	41.0
66-67	36.74342508372835	38.0	35.0	40.0	31.5	41.0
68-69	36.507626415794064	37.5	35.0	40.0	31.0	41.0
70-71	36.193315371018514	37.0	35.0	39.5	31.0	41.0
72-73	35.87706593591466	36.5	34.5	39.0	31.0	41.0
74-75	35.478090915553956	36.0	34.0	39.0	30.5	40.0
76-77	33.42828198286607	34.5	32.0	36.5	27.5	39.0
78-79	34.630670601702334	35.0	34.0	37.0	30.0	39.0
80-81	34.6371233965469	35.0	34.0	37.0	30.0	39.0
82-83	34.3515220114687	35.0	34.0	36.5	30.0	39.0
84-85	34.093984080902786	35.0	34.0	36.0	30.0	37.0
86-87	33.886531447589704	35.0	34.0	36.0	30.0	37.0
88-89	33.64851070193207	35.0	33.5	35.0	30.0	36.5
90-91	33.48463768898388	35.0	33.0	35.0	29.5	36.0
92-93	33.41875525177035	35.0	33.0	35.0	29.0	36.0
94-95	33.39134755238173	35.0	33.0	35.0	30.0	36.0
96-97	33.425996866077696	35.0	33.0	35.0	30.0	35.5
98-99	33.45242190225798	35.0	33.0	35.0	30.0	35.0
100-101	32.16026864639515	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	15.0
20	60.0
21	209.0
22	888.0
23	3073.0
24	8432.0
25	18636.0
26	34648.0
27	56727.0
28	85083.0
29	118939.0
30	158881.0
31	207397.0
32	273102.0
33	372606.0
34	538512.0
35	828756.0
36	1469609.0
37	2687685.0
38	3117902.0
39	1251035.0
40	20140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56822122614307	20.296807570975677	14.125158489242374	24.00981271363888
2	14.39416912108441	21.120814232719685	42.01945996292155	22.46555668327435
3	18.344691130165142	26.00816239909089	30.248484558174578	25.398661912569388
4	12.388783054037154	17.928959081548605	39.147262985130546	30.53499487928369
5	13.062671192306732	37.921067199320746	35.87347368291957	13.142787925452955
6	29.958459310822523	38.16209444772426	18.86085748734419	13.018588754109034
7	26.439729553117804	32.53549328721788	23.407408895163933	17.617368264500385
8	23.960485579077766	37.50685748874208	21.304912232586823	17.227744699593337
9	25.33934311193204	17.001512931368513	21.41807455310581	36.24106940359364
10-11	23.987923575342617	26.96251898605596	29.188700978294552	19.860856460306866
12-13	24.73807934823697	24.434879396547046	29.363320537590926	21.46372071762506
14-15	21.834983738270605	25.908514402715987	26.890003629911806	25.3664982291016
16-17	21.515721958058663	29.034846068639553	27.56427848274058	21.88515349056121
18-19	21.54570420260567	28.33382530505542	29.113951892941742	21.006518599397165
20-21	22.25253645910704	27.531036716240127	29.015232063439996	21.20119476121284
22-23	21.969212801505297	27.42293812273949	28.894779259604253	21.713069816150956
24-25	21.727872711979934	27.5569314058272	28.971556367054735	21.74363951513813
26-27	21.75047898097839	27.74049714412386	28.815162146484024	21.693861728413726
28-29	21.643310927356808	27.85387400422868	28.845994162229573	21.656820906184944
30-31	21.9862000280668	27.653947282985968	28.757964646078193	21.60188804286903
32-33	21.737998948576546	27.81985581691201	28.714133244798685	21.728011989712762
34-35	21.81411437538607	27.85781324353277	28.753905991858087	21.57416638922307
36-37	21.85901906495972	27.784289201706535	28.671343509354713	21.685348223979027
38-39	21.70927312951704	27.924495859879904	28.727585927652072	21.63864508295098
40-41	21.9428033772474	27.75957322051456	28.6299200314285	21.667703370809534
42-43	21.80500304391334	27.889280377584463	28.46855734548815	21.837159233014045
44-45	21.899482843058326	27.97298230520245	28.316390113131224	21.811144738608
46-47	21.951746625894607	27.932296506729394	28.116014448294358	21.999942419081638
48-49	21.907420809393987	28.020051569492015	28.117572812644443	21.954954808469555
50-51	21.91002696220148	28.121063689802174	28.02228537208237	21.94662397591398
52-53	22.049810397309162	28.04190124679448	27.86333614723628	22.044952208660078
54-55	22.01654894394152	27.983771675265707	27.913308873922198	22.086370506870576
56-57	22.013990952266976	28.040203465198672	27.94693704368192	21.998868538852438
58-59	21.986447333548934	28.100070372632107	27.897863377739405	22.01561891607955
60-61	22.088778819630946	28.04897358060027	27.909264075560912	21.952983524207873
62-63	22.069278180397287	28.096394754845388	27.850408847021495	21.983918217735827
64-65	22.008959607528052	28.067786578942012	27.82516211868245	22.09809169484749
66-67	22.073846567864173	28.060951614245553	27.72433267564946	22.14086914224081
68-69	22.008387768292987	28.07010589554834	27.81464172763442	22.10686460852425
70-71	22.03734512207546	27.866926465622953	27.884043113256308	22.211685299045275
72-73	22.104742840383473	27.877203817670686	27.82373182085667	22.19432152108917
74-75	21.949281401282917	27.99359278290787	27.83050325897963	22.226622556829582
76-77	22.064902478648698	27.948523095714933	27.753701806039295	22.232872619597078
78-79	22.02235331825592	27.93664758999813	27.758181283977468	22.282817807768478
80-81	22.027116079577432	27.983621908754934	27.762118368613304	22.22714364305433
82-83	22.080553076183147	27.860252365189485	27.882966389718682	22.176228168908683
84-85	22.190406551131165	27.86863065533417	27.796333212479812	22.144629581054858
86-87	22.040825057899045	27.85027630751235	27.887635650204185	22.221262984384417
88-89	22.103268518001	27.80068412366185	27.817535164630737	22.27851219370641
90-91	22.14524517941808	27.8414004526568	27.82808844113199	22.185265926793136
92-93	22.130493783667294	27.76252615150257	27.91312485608063	22.19385520874951
94-95	22.06672358586174	27.77171236124382	27.924164212816226	22.237399840078208
96-97	22.05315123534574	27.82271541916676	27.94495925163028	22.179174093857217
98-99	22.301776718816928	28.34155131845442	28.05021574999249	21.30645621273616
100-101	23.003479831984283	30.921520154756184	25.23057381730075	20.844426195958782
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1877.0
1	1729.0
2	2535.5
3	3778.5
4	5104.0
5	8166.0
6	11022.5
7	13546.5
8	15688.5
9	15801.0
10	14110.5
11	12419.5
12	13277.0
13	17203.0
14	26131.5
15	38030.5
16	47483.5
17	52819.5
18	54239.5
19	52869.5
20	50644.5
21	49384.0
22	51018.0
23	58135.0
24	71080.5
25	88940.0
26	111807.0
27	136332.5
28	162012.0
29	188870.0
30	213978.0
31	238594.5
32	263281.0
33	286716.0
34	309712.0
35	331615.0
36	355533.0
37	377916.5
38	393875.5
39	407182.0
40	422557.0
41	436550.5
42	446134.5
43	457010.0
44	467822.0
45	475534.0
46	478808.5
47	479089.0
48	478120.0
49	470530.0
50	459066.0
51	444872.5
52	423381.0
53	400596.0
54	376287.0
55	347298.5
56	317611.5
57	286631.0
58	254560.0
59	220392.0
60	184615.5
61	151071.0
62	119935.0
63	93091.5
64	70226.5
65	53049.0
66	40654.0
67	30872.5
68	23271.5
69	16710.5
70	11718.5
71	8439.5
72	5970.5
73	4160.0
74	2924.5
75	2000.5
76	1343.5
77	925.0
78	666.0
79	479.0
80	330.5
81	216.5
82	151.0
83	102.0
84	72.0
85	53.5
86	46.0
87	38.5
88	24.5
89	15.5
90	11.5
91	7.5
92	4.5
93	3.0
94	3.0
95	3.0
96	3.0
97	3.0
98	1.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004630147628835635
3	1.066444751363294E-4
4	7.998335635224706E-5
5	8.887039594694118E-5
6	0.0
7	1.7774079189388235E-5
8	3.9102974216654115E-4
9	1.1553151473102353E-4
10-11	3.554815837877647E-5
12-13	2.7105470763817056E-4
14-15	1.3330559392041175E-5
16-17	4.7101309851878823E-4
18-19	4.443519797347059E-6
20-21	3.5549363646394214E-5
22-23	0.0
24-25	0.0
26-27	4.44684475242303E-5
28-29	8.899853508411251E-6
30-31	8.912917718929447E-5
32-33	4.244998051769315E-4
34-35	0.0
36-37	3.829204159831162E-4
38-39	9.663173527731584E-4
40-41	0.0
42-43	0.0
44-45	2.792114770063765E-4
46-47	9.408645905799508E-6
48-49	4.278913869029578E-5
50-51	3.0737554832315834E-4
52-53	5.818156147771663E-4
54-55	0.0030005141500918852
56-57	0.0031944954195584022
58-59	0.004067963374136738
60-61	8.030378467191661E-4
62-63	5.1031241887627265E-6
64-65	4.742965550552357E-4
66-67	0.0
68-69	0.0
70-71	1.6001849387073163E-5
72-73	1.2951117786692823E-4
74-75	4.3663720147865005E-5
76-77	0.0
78-79	0.0
80-81	4.6957262724244263E-4
82-83	0.0
84-85	0.0
86-87	5.8768822551947965E-6
88-89	0.0
90-91	9.378829247400991E-4
92-93	1.4760939209039993E-4
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	230.0
20-21	831.0
22-23	1726.0
24-25	4320.0
26-27	6525.0
28-29	12327.0
30-31	28853.0
32-33	34689.0
34-35	47233.0
36-37	72228.0
38-39	86905.0
40-41	86500.0
42-43	97470.0
44-45	114866.0
46-47	113584.0
48-49	108045.0
50-51	99620.0
52-53	96117.0
54-55	102982.0
56-57	105741.0
58-59	105261.0
60-61	103524.0
62-63	99176.0
64-65	102178.0
66-67	108369.0
68-69	111279.0
70-71	109949.0
72-73	104992.0
74-75	106066.0
76-77	103556.0
78-79	111189.0
80-81	112618.0
82-83	108717.0
84-85	109016.0
86-87	114308.0
88-89	122659.0
90-91	136517.0
92-93	133281.0
94-95	150232.0
96-97	228636.0
98-99	946093.0
100-101	6703933.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.98803823776394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.6216751278553	76.16770352698624
2	7.204588152199685	12.24607626732976
3	1.818375484994785	4.63620495748048
4	0.6288542953461875	2.137803715954555
5	0.29631963549309037	1.259181225594352
6	0.14970840789394776	0.7634054336763356
7	0.08319530510700536	0.49494240401456263
8	0.053795776091509366	0.3657597980396309
9	0.030684320441895438	0.23470201791140505
>10	0.10998398693793181	1.4550820911351232
>50	0.0023425348217808416	0.12885103957300262
>100	4.264684671646822E-4	0.06796588496489328
>500	3.366972280525239E-5	0.02435151886792799
>1k	1.68346269217132E-5	0.01797011847175561
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.6661118784082354E-5	0.0	0.0	0.0
3	0.0	2.6661118784082354E-5	0.0	0.0	0.0
4	0.0	5.332223756816471E-5	0.0	0.0	0.0
5	0.0	7.109631675755294E-5	0.0	0.0	0.0
6	0.0	7.109631675755294E-5	0.0	0.0	0.0
7	0.0	7.998335635224705E-5	0.0	0.0	0.0
8	0.0	8.887039594694118E-5	0.0	0.0	0.0
9	0.0	1.3330559392041175E-4	0.0	1.7774079189388235E-5	0.0
10-11	0.0	1.3330559392041175E-4	0.0	2.6661118784082354E-5	0.0
12-13	0.0	1.3330559392041175E-4	0.0	2.6661118784082354E-5	0.0
14-15	0.0	2.266195096647E-4	0.0	3.9991678176123525E-5	4.443519797347059E-6
16-17	0.0	2.4439358885408824E-4	0.0	5.332223756816471E-5	2.2217598986735294E-5
18-19	0.0	2.7105470763817056E-4	0.0	6.220927716285883E-5	3.554815837877647E-5
20-21	0.0	2.888287868275588E-4	0.0	6.220927716285883E-5	3.554815837877647E-5
22-23	0.0	3.2882046500368233E-4	0.0	9.77574355416353E-5	6.220927716285883E-5
24-25	0.0	3.599251035851117E-4	0.0	1.732972720965353E-4	7.998335635224705E-5
26-27	0.0	3.9102974216654115E-4	0.0	2.9771582642225294E-4	7.998335635224705E-5
28-29	0.0	4.0436030155858234E-4	0.0	0.0012308549838651352	7.998335635224705E-5
30-31	0.0	4.976742173028706E-4	0.0	0.002883844348478241	7.998335635224705E-5
32-33	0.0	5.110047766949117E-4	0.0	0.005932098929458323	7.998335635224705E-5
34-35	0.0	5.909881330471588E-4	0.0	0.010193434415114153	1.0664447513632941E-4
36-37	0.0	6.576409300073647E-4	0.0	0.015930018473489203	1.0664447513632941E-4
38-39	0.0	6.976326081834883E-4	0.0	0.02579018890380233	1.0664447513632941E-4
40-41	1.7774079189388235E-5	7.642854051436941E-4	0.0	0.0345972451421442	1.0664447513632941E-4
42-43	1.7774079189388235E-5	8.042770833198176E-4	0.0	0.04148470082803214	1.0664447513632941E-4
44-45	1.7774079189388235E-5	8.975909990641059E-4	0.0	0.049794082849071136	1.0664447513632941E-4
46-47	2.6661118784082354E-5	9.731308356190058E-4	0.0	0.059258780017420375	1.1553151473102353E-4
48-49	2.6661118784082354E-5	9.820178752137E-4	0.0	0.06814137609231714	1.1997503452837059E-4
50-51	2.6661118784082354E-5	0.0011108799493367647	0.0	0.07666404706362881	1.2886207412306472E-4
52-53	2.6661118784082354E-5	0.001128654028526153	0.0	0.08471570493642167	1.466361533124529E-4
54-55	2.6661118784082354E-5	0.001128654028526153	0.0	0.0921497135573833	1.599667127044941E-4
56-57	2.6661118784082354E-5	0.001128654028526153	0.0	0.0995615045793582	1.599667127044941E-4
58-59	3.554815837877647E-5	0.001128654028526153	0.0	0.10640896858707001	1.599667127044941E-4
60-61	3.554815837877647E-5	0.0011553151473102353	0.0	0.11290983805058877	1.599667127044941E-4
62-63	3.554815837877647E-5	0.0011642021869049293	0.0	0.1186641961881532	1.7774079189388235E-4
64-65	4.443519797347059E-5	0.0013330559392041176	0.0	0.12472960071153194	1.7774079189388235E-4
66-67	5.332223756816471E-5	0.0013419429787988116	0.0	0.13120825257606394	1.9995839088061767E-4
68-69	5.332223756816471E-5	0.0013774911371775883	0.0	0.1376913479603933	2.1773247007000586E-4
70-71	5.332223756816471E-5	0.0013774911371775883	0.0	0.14356568113248613	2.2217598986735293E-4
72-73	5.332223756816471E-5	0.0013774911371775883	0.0	0.14839134363240503	2.2217598986735293E-4
74-75	5.332223756816471E-5	0.0014263698549484057	0.0	0.15251493000434307	2.2217598986735293E-4
76-77	5.332223756816471E-5	0.0014708050529218765	0.0	0.1562252690351279	2.2217598986735293E-4
78-79	5.332223756816471E-5	0.0014752485727192236	0.0	0.15956679592273287	2.2217598986735293E-4
80-81	5.332223756816471E-5	0.0014752485727192236	0.0	0.16119756768835924	2.266195096647E-4
82-83	5.332223756816471E-5	0.0014796920925165707	0.0	0.16179299934120375	2.488371086514353E-4
84-85	5.332223756816471E-5	0.0014974661717059587	0.0	0.1624684143504005	2.488371086514353E-4
86-87	5.332223756816471E-5	0.0015419013696794295	0.0	0.1627705736966201	2.488371086514353E-4
88-89	5.332223756816471E-5	0.0016218847260316763	0.0	0.1628816616915538	2.5772414824612943E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12250	0.0	44.937027	1
GTATCAA	21295	0.0	34.69724	1
TCAACGC	24880	0.0	28.818214	4
ATCAACG	25015	0.0	28.696314	3
CAACGCA	25530	0.0	28.0679	5
AACGCAG	26325	0.0	27.316355	6
TATCAAC	26675	0.0	27.132078	2
ACGCAGA	31025	0.0	23.05619	7
CGCAGAG	31595	0.0	22.693478	8
GTACATG	31160	0.0	21.687986	1
TACATGG	30200	0.0	21.50042	2
ACATGGG	30265	0.0	20.8561	3
GCAGAGT	38375	0.0	18.6838	9
GTGGTAT	6890	0.0	18.554754	1
CATGGGG	21665	0.0	18.109898	4
GAGTACT	21715	0.0	17.661362	12-13
AGAGTAC	33435	0.0	17.230957	10-11
CAGAGTA	36675	0.0	16.975729	10-11
TGGTATC	6875	0.0	16.515724	2
GTACTTT	24605	0.0	15.638831	14-15
>>END_MODULE