FastQCFastQC Report
Fri 27 May 2016
SRR2138791_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138791_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11912788
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127550.043.190791
GTATCAA219100.033.108121
TCAACGC248300.028.3477974
ATCAACG252650.027.926453
CAACGCA255100.027.5260645
AACGCAG259100.027.1988326
TATCAAC274200.026.0862832
ACGCAGA303600.023.1844147
CGCAGAG308200.022.778
GTACATG300250.020.3273851
TACATGG298600.019.6916542
ACATGGG298650.018.8407553
GCAGAGT377500.018.5563969
GAGTACT219350.017.30320512-13
AGAGTAC334850.016.87314810-11
CATGGGG216850.016.8515264
CAGAGTA371450.016.70838710-11
GTGGTAT74150.016.371081
TGGTATC73700.015.09851552
GTACTTT257300.014.99725714-15
Produced by FastQC (version 0.11.5)