FastQCFastQC Report
Fri 27 May 2016
SRR2138790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9801889
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100000.042.399741
GTATCAA168350.033.78721
CTTATAC87350.031.149721
TCAACGC190000.028.7721064
ATCAACG192050.028.4649853
CAACGCA196100.027.7707045
AACGCAG202050.027.056596
TATCAAC208750.026.6288032
TATACAC127450.024.6224883
ACGCAGA237650.022.9508297
CGCAGAG242000.022.5727718
TTATACA131350.022.0815032
GTACATG258050.021.0883791
TACATGG257800.020.6072182
ACATCTC140750.019.835288
ACACATC137450.019.7650016
ACATGGG261500.019.3572243
TACACAT163050.019.1696855
GCAGAGT297200.018.3660229
TATGCCG83600.017.46571442-43
Produced by FastQC (version 0.11.5)