##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138790_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9801889
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36555565973049	33.0	31.0	34.0	31.0	34.0
2	32.544140012195605	34.0	31.0	34.0	31.0	34.0
3	32.6665533551747	34.0	31.0	34.0	31.0	34.0
4	36.175756836258806	37.0	35.0	37.0	35.0	37.0
5	36.007221057083996	37.0	35.0	37.0	35.0	37.0
6	36.04900953275435	37.0	35.0	37.0	35.0	37.0
7	36.02886923122676	37.0	35.0	37.0	35.0	37.0
8	36.016669337920476	37.0	35.0	37.0	35.0	37.0
9	37.86473117579683	39.0	38.0	39.0	35.0	39.0
10-11	37.748602896849775	39.0	37.5	39.0	35.0	39.0
12-13	37.76852778071655	39.0	37.5	39.0	35.0	39.0
14-15	39.106492738287486	40.0	38.5	41.0	36.0	41.0
16-17	39.057316809035484	40.0	38.0	41.0	36.0	41.0
18-19	39.11510709823382	40.0	38.5	41.0	36.0	41.0
20-21	39.117137088477406	40.0	39.0	41.0	36.0	41.0
22-23	39.0813333396878	40.0	39.0	41.0	36.0	41.0
24-25	39.06615945543058	40.0	39.0	41.0	36.0	41.0
26-27	38.970255095562536	40.0	38.5	41.0	35.5	41.0
28-29	38.89126532805187	40.0	38.0	41.0	35.0	41.0
30-31	38.75396699944994	40.0	38.0	41.0	35.0	41.0
32-33	38.67055641493701	40.0	38.0	41.0	35.0	41.0
34-35	38.62751046945898	40.0	38.0	41.0	35.0	41.0
36-37	38.526614076764744	40.0	38.0	41.0	34.5	41.0
38-39	38.488569170623684	40.0	38.0	41.0	34.0	41.0
40-41	38.498716105814054	40.0	38.0	41.0	34.0	41.0
42-43	38.46632943153455	40.0	38.0	41.0	34.0	41.0
44-45	38.35907989998251	40.0	38.0	41.0	34.0	41.0
46-47	38.30204447350515	40.0	38.0	41.0	34.0	41.0
48-49	38.281098034799896	40.0	38.0	41.0	34.0	41.0
50-51	38.192085649835775	40.0	38.0	41.0	33.5	41.0
52-53	38.060569036861835	40.0	37.0	41.0	33.0	41.0
54-55	37.88712381475612	40.0	37.0	41.0	33.0	41.0
56-57	37.75479847570995	39.0	37.0	41.0	33.0	41.0
58-59	37.52808708625067	39.0	36.0	41.0	32.5	41.0
60-61	37.357855612734454	39.0	36.0	40.5	32.0	41.0
62-63	37.14563097654175	39.0	35.0	40.0	32.0	41.0
64-65	36.82806654768247	38.5	35.0	40.0	31.0	41.0
66-67	36.7467432563868	38.0	35.0	40.0	32.0	41.0
68-69	36.513566239257706	37.5	35.0	40.0	31.5	41.0
70-71	36.193343110920104	37.0	35.0	39.5	31.0	41.0
72-73	35.87434633100462	36.5	34.5	39.0	31.0	41.0
74-75	35.472053252987735	36.0	34.0	39.0	31.0	40.0
76-77	33.43672849870043	34.5	32.0	36.5	27.5	39.0
78-79	34.611423244297384	35.0	34.0	37.0	30.0	39.0
80-81	34.616634561068246	35.0	34.0	37.0	30.0	39.0
82-83	34.33910229867813	35.0	34.0	36.0	30.0	38.5
84-85	34.08932230134778	35.0	34.0	36.0	30.0	37.0
86-87	33.887919606014805	35.0	34.0	35.5	30.0	37.0
88-89	33.660825181171084	35.0	33.5	35.0	30.0	36.5
90-91	33.50099514735038	35.0	33.0	35.0	29.5	36.0
92-93	33.43974747798239	35.0	33.0	35.0	30.0	36.0
94-95	33.41777127377244	35.0	33.0	35.0	30.0	36.0
96-97	33.45241659723648	35.0	33.0	35.0	30.0	35.5
98-99	33.47825401989728	35.0	33.0	35.0	30.0	35.0
100-101	32.22901836065201	34.0	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	7.0
19	20.0
20	50.0
21	204.0
22	797.0
23	2629.0
24	7235.0
25	15677.0
26	29411.0
27	48594.0
28	72374.0
29	101289.0
30	135039.0
31	176033.0
32	232482.0
33	317217.0
34	458141.0
35	704728.0
36	1263369.0
37	2350394.0
38	2763702.0
39	1107656.0
40	14839.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.26259744422733	20.336386180255666	14.012645929779454	24.38837044573755
2	14.405167907933276	20.935032530513713	42.161421011439884	22.49837855011313
3	18.365117878905078	26.011435968259562	30.265915287076023	25.357530865759337
4	12.442582490230453	18.118009510004605	38.65233922281095	30.78706877695399
5	13.219978934661949	37.723166281094535	35.79632242128146	13.26053236296206
6	29.950869674202597	37.91901744653505	18.86520037107133	13.264912508191024
7	26.425531489443664	32.314121524343065	23.266782889174003	17.993564097039265
8	23.929254937056225	37.548407988171206	21.35419838901725	17.16813868575532
9	25.19034376192056	17.074383043808677	21.23037530283759	36.504897891433174
10-11	24.11767982563352	26.843716983388095	29.02178805625531	20.01681513472308
12-13	24.85324084203011	24.150629189352202	29.17442136101026	21.821708607607423
14-15	21.928119554938757	25.694419860175906	26.46451137418744	25.9129492106979
16-17	21.87558550866034	28.915921453731062	27.112790704970152	22.09570233263845
18-19	21.942795924336625	28.12744563828462	28.547782983463698	21.381975453915057
20-21	22.574003869987518	27.29772091892921	28.386131952984734	21.74214325809854
22-23	22.237852590750588	27.46742165870135	28.269459549483166	22.02526620106489
24-25	22.16687196579958	27.578686378104873	28.342460385091506	21.91198127100404
26-27	22.18966959442942	27.749436091444124	28.218193931660444	21.84270038246601
28-29	21.97075085480786	27.780343725267425	28.24565710407677	22.00324831584794
30-31	22.5014106988011	27.540662145950414	28.171877597898405	21.786049557350086
32-33	21.87900346231106	27.880021103981644	28.233112081526674	22.00786335218062
34-35	22.132172449228698	27.79893339833724	28.29049455337388	21.778399599060183
36-37	22.06673154200989	27.81299234596208	28.191602654412918	21.928673457615112
38-39	21.973140152110602	27.634764734065143	28.38423975523276	22.007855358591495
40-41	22.150301643365534	27.485662886654712	28.336961125805615	22.027074344174142
42-43	22.188120359925943	27.521083582916418	28.216796589934027	22.073999467223615
44-45	22.072937051106926	27.747429360152896	28.07517726642824	22.10445632231193
46-47	22.336600757295805	27.567784835257463	27.746836611948034	22.3487777954987
48-49	22.19344731819057	27.665377996869605	27.780626775162965	22.36054790977686
50-51	22.082856304839815	27.78083612609148	27.876930414686925	22.25937715438178
52-53	22.22246336594852	27.73534456150729	27.89496182017373	22.14723025237046
54-55	22.188486807496545	27.697279003431714	27.721914758704642	22.392319430367095
56-57	22.334295117277986	27.856581740321534	27.576131896051454	22.232991246349027
58-59	22.169149501443325	27.969742671529335	27.762796253342515	22.09831157368483
60-61	22.38016759073834	28.072039562787438	27.48297385511937	22.064818991354855
62-63	22.220159015964303	28.196808323868805	27.45356853332029	22.129464126846603
64-65	22.26172819622254	28.070778088133352	27.43529313922039	22.232200576423715
66-67	22.320941293520267	27.989595782500288	27.38698835819628	22.302474565783168
68-69	22.253859653639662	27.907753827916526	27.49157676174917	22.34680975669464
70-71	22.291205556805085	27.700683553903076	27.57076004506271	22.437350844229126
72-73	22.324386918459847	27.751683770507963	27.498188238638143	22.425741072394043
74-75	22.178986072128247	27.880800504629526	27.51245304279863	22.427760380443594
76-77	22.3515489721221	27.826118164431858	27.40634459950281	22.415988263943238
78-79	22.260897133025622	27.830553769283423	27.40783216174023	22.500716935950724
80-81	22.264109652146274	27.856317075752585	27.429004097077552	22.450569175023592
82-83	22.2805547119883	27.801493537680255	27.54718811163271	22.370763638698733
84-85	22.401368100237047	27.81750761937013	27.41240094818828	22.36872333220454
86-87	22.246633378809012	27.826841313938637	27.531049342630777	22.39547596462157
88-89	22.353419875530143	27.806448800270502	27.44035526897744	22.399776055221913
90-91	22.363464588450448	27.796737205809645	27.478091284034146	22.36170692170576
92-93	22.325975116662832	27.78033956288224	27.514891765595443	22.378793554859485
94-95	22.246953757320703	27.84042867713262	27.51402443586842	22.398593129678247
96-97	22.256642024343705	27.842694898626185	27.57581969423975	22.324843382790355
98-99	22.46385058162778	28.414083271554386	27.667387313256146	21.454678833561687
100-101	26.558847426545682	35.43408869340411	28.253946639109163	9.75311724094105
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1906.0
1	1732.0
2	2238.0
3	3120.0
4	4311.0
5	6989.0
6	9193.5
7	10629.5
8	11540.5
9	11192.5
10	10165.5
11	9274.0
12	10037.0
13	13083.0
14	19980.5
15	29249.0
16	36706.0
17	40939.5
18	41939.5
19	40694.5
20	38996.0
21	37931.5
22	39440.0
23	45460.0
24	56169.0
25	71666.5
26	90511.5
27	111066.0
28	133038.0
29	155364.0
30	176039.0
31	195814.5
32	215705.5
33	235489.5
34	256082.0
35	276132.0
36	297954.0
37	318844.0
38	335796.0
39	350711.0
40	364882.0
41	378940.0
42	390626.0
43	401650.0
44	411958.0
45	421039.0
46	432213.5
47	441268.0
48	440549.5
49	435227.0
50	428470.0
51	414913.0
52	392997.5
53	368183.0
54	344064.0
55	318126.0
56	292069.0
57	265022.0
58	235453.5
59	203892.0
60	171693.5
61	141259.5
62	112217.5
63	86413.0
64	64857.0
65	48318.0
66	36212.0
67	27456.5
68	20871.5
69	14940.5
70	10453.5
71	7371.0
72	5045.5
73	3379.5
74	2240.5
75	1515.0
76	1073.5
77	764.5
78	547.0
79	400.0
80	282.0
81	189.5
82	131.0
83	88.0
84	61.0
85	46.0
86	32.5
87	24.0
88	17.5
89	11.0
90	6.5
91	5.0
92	4.0
93	2.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0048562067985058795
3	8.16169209832921E-5
4	1.0202115122911512E-5
5	5.1010575614557566E-5
6	0.0
7	1.0202115122911512E-5
8	4.6929729565392954E-4
9	1.8363807221240722E-4
10-11	5.1010575614557566E-5
12-13	3.1116451124880116E-4
14-15	2.0404230245823024E-5
16-17	4.948025834612083E-4
18-19	0.0
20-21	4.591093492874853E-5
22-23	0.0
24-25	0.0
26-27	1.1740371185617207E-4
28-29	0.0
30-31	1.0743297550819761E-4
32-33	5.23338243306414E-4
34-35	0.0
36-37	4.140741955741783E-4
38-39	0.0011943640827495074
40-41	0.0
42-43	0.0
44-45	4.286859719321075E-4
46-47	0.0
48-49	1.0958851867391098E-5
50-51	5.538253436458566E-5
52-53	6.488464349412307E-4
54-55	0.003277126100535223
56-57	0.0036511258409374168
58-59	0.004243273703004949
60-61	0.0010062597547230148
62-63	5.921191078375609E-6
64-65	6.471108567406213E-4
66-67	0.0
68-69	0.0
70-71	1.2440012703740972E-5
72-73	2.0141131425540187E-4
74-75	4.4574060151165926E-5
76-77	0.0
78-79	0.0
80-81	6.07528399476337E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.00131999736000528
92-93	1.79312681580987E-4
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	178.0
20-21	671.0
22-23	1495.0
24-25	3267.0
26-27	5545.0
28-29	10783.0
30-31	27374.0
32-33	32585.0
34-35	44125.0
36-37	68682.0
38-39	81542.0
40-41	80289.0
42-43	89470.0
44-45	103221.0
46-47	102842.0
48-49	98620.0
50-51	90690.0
52-53	87915.0
54-55	96401.0
56-57	101768.0
58-59	101823.0
60-61	103710.0
62-63	100062.0
64-65	99830.0
66-67	107178.0
68-69	98991.0
70-71	95913.0
72-73	91951.0
74-75	92960.0
76-77	90174.0
78-79	95648.0
80-81	97801.0
82-83	92964.0
84-85	92532.0
86-87	96350.0
88-89	103141.0
90-91	114953.0
92-93	111694.0
94-95	127282.0
96-97	197083.0
98-99	810756.0
100-101	5751630.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.39313901759122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.84843823155356	76.72440176420511
2	7.112061712595576	12.146425490507239
3	1.7159128873877965	4.395815632043473
4	0.6244681848144111	2.1330119407167945
5	0.27445145910426205	1.17181358004355
6	0.14853620243040067	0.7610383549970566
7	0.08434934104145855	0.5041998503916838
8	0.05268599086029652	0.35992177134502723
9	0.028714175100313544	0.22067941915048778
>10	0.1079800851366248	1.4023739311623307
>50	0.0019505481177658304	0.1123308151452755
>100	4.3204118800116664E-4	0.05744504230974725
>500	1.914066957263544E-5	0.010542407982202646
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0202115122911512E-5	0.0	1.0202115122911512E-5	0.0
3	0.0	1.0202115122911512E-5	0.0	1.0202115122911512E-5	0.0
4	0.0	5.101057561455756E-5	0.0	1.0202115122911512E-5	0.0
5	0.0	8.16169209832921E-5	0.0	1.0202115122911512E-5	0.0
6	4.080846049164605E-5	8.16169209832921E-5	0.0	1.0202115122911512E-5	2.0404230245823024E-5
7	4.080846049164605E-5	8.16169209832921E-5	0.0	2.0404230245823024E-5	2.0404230245823024E-5
8	5.101057561455756E-5	1.1222326635202663E-4	0.0	2.0404230245823024E-5	3.060634536873454E-5
9	5.101057561455756E-5	2.550528780727878E-4	0.0	4.080846049164605E-5	4.080846049164605E-5
10-11	5.101057561455756E-5	2.550528780727878E-4	0.0	5.611163317601332E-5	4.080846049164605E-5
12-13	5.101057561455756E-5	2.652549931956993E-4	0.0	6.121269073746908E-5	4.080846049164605E-5
14-15	5.611163317601332E-5	3.3666979905607993E-4	0.0	6.121269073746908E-5	8.16169209832921E-5
16-17	9.692009366765936E-5	3.3666979905607993E-4	0.0	8.16169209832921E-5	9.181903610620361E-5
18-19	1.0202115122911512E-4	3.5707402930190295E-4	0.0	9.692009366765936E-5	1.0202115122911512E-4
20-21	1.0202115122911512E-4	4.080846049164605E-4	0.0	1.5303172684367268E-4	1.1222326635202664E-4
22-23	1.0202115122911512E-4	4.6929729565392954E-4	0.0	1.632338419665842E-4	1.5303172684367268E-4
24-25	1.0712220879057087E-4	4.999036410226641E-4	0.0	1.7853701465095145E-4	1.9894124489677447E-4
26-27	1.1222326635202663E-4	5.203078712684872E-4	0.0	2.4995182051133205E-4	2.2444653270405326E-4
28-29	1.1222326635202663E-4	5.203078712684872E-4	0.0	7.141480586038059E-4	2.2444653270405326E-4
30-31	1.2242538147493815E-4	6.019247922517793E-4	0.0	0.0020608272548281255	2.2444653270405326E-4
32-33	1.3262749659784966E-4	6.121269073746907E-4	0.0	0.005172472367316137	2.550528780727878E-4
34-35	1.4793066928221694E-4	6.733395981121599E-4	0.0	0.008957457077916307	2.8565922344152235E-4
36-37	1.632338419665842E-4	7.396533464110846E-4	0.0	0.014889987021889352	2.8565922344152235E-4
38-39	1.632338419665842E-4	7.549565190954519E-4	0.0	0.024260629762283575	3.264676839331684E-4
40-41	1.734359570894957E-4	8.059670947100095E-4	0.0	0.034177085661753565	3.3666979905607993E-4
42-43	1.734359570894957E-4	8.263713249558324E-4	0.0	0.04291519726452728	3.4177085661753567E-4
44-45	1.8873912977386298E-4	9.538977639922264E-4	0.0	0.05182674482439048	3.519729717404472E-4
46-47	1.9384018733531873E-4	0.0010508178576598857	0.0	0.061926838796072886	3.9278143223209325E-4
48-49	1.9384018733531873E-4	0.0010559189152213415	0.0	0.0713331889393973	4.284888351622835E-4
50-51	2.0404230245823024E-4	0.0012242538147493815	0.0	0.08021413015389177	4.284888351622835E-4
52-53	2.0404230245823024E-4	0.0012395569874337486	0.0	0.08864107724541667	4.5909518053101805E-4
54-55	2.0404230245823024E-4	0.0012854665054868506	0.0	0.09728226875452273	4.897015258997526E-4
56-57	2.0404230245823024E-4	0.0013160728508555852	0.0	0.10489304663621471	4.897015258997526E-4
58-59	2.0404230245823024E-4	0.0013313760235399523	0.0	0.11261094672669728	4.897015258997526E-4
60-61	2.0404230245823024E-4	0.0013925887142774214	0.0	0.11942085857124071	4.948025834612083E-4
62-63	2.2444653270405326E-4	0.0014282961172076118	0.0	0.12620526512797686	5.305099863913987E-4
64-65	2.2444653270405326E-4	0.001591529959174196	0.0	0.13245406064076015	5.407121015143101E-4
66-67	2.6015393563424353E-4	0.001611934189420019	0.0	0.1393098820033567	5.509142166372216E-4
68-69	2.652549931956993E-4	0.0016884500528418552	0.0	0.14641055412890314	5.509142166372216E-4
70-71	2.805581658800666E-4	0.0017343595708949572	0.0	0.15280217925340717	5.509142166372216E-4
72-73	3.0606345368734537E-4	0.0017547638011407801	0.0	0.15784202412412546	6.529353678663368E-4
74-75	3.0606345368734537E-4	0.0018108754343167935	0.0	0.16216772093623993	6.529353678663368E-4
76-77	3.162655688102569E-4	0.0018720881250542626	0.0	0.1658149770926808	6.580364254277925E-4
78-79	3.162655688102569E-4	0.0018975934128615413	0.0	0.16924288777397906	6.682385405507041E-4
80-81	3.162655688102569E-4	0.0018975934128615413	0.0	0.17093133782682093	6.784406556736157E-4
82-83	3.2136662637171265E-4	0.0018975934128615413	0.0	0.17151795944638837	7.039459434808943E-4
84-85	3.264676839331684E-4	0.0019128965855459085	0.0	0.17201276202984955	7.039459434808943E-4
86-87	3.3666979905607993E-4	0.0019486039884760989	0.0	0.17223210750499213	7.039459434808943E-4
88-89	3.3666979905607993E-4	0.0020302209094593907	0.0	0.17231372442597545	7.039459434808943E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10000	0.0	42.39974	1
GTATCAA	16835	0.0	33.7872	1
CTTATAC	8735	0.0	31.14972	1
TCAACGC	19000	0.0	28.772106	4
ATCAACG	19205	0.0	28.464985	3
CAACGCA	19610	0.0	27.770704	5
AACGCAG	20205	0.0	27.05659	6
TATCAAC	20875	0.0	26.628803	2
TATACAC	12745	0.0	24.622488	3
ACGCAGA	23765	0.0	22.950829	7
CGCAGAG	24200	0.0	22.572771	8
TTATACA	13135	0.0	22.081503	2
GTACATG	25805	0.0	21.088379	1
TACATGG	25780	0.0	20.607218	2
ACATCTC	14075	0.0	19.83528	8
ACACATC	13745	0.0	19.765001	6
ACATGGG	26150	0.0	19.357224	3
TACACAT	16305	0.0	19.169685	5
GCAGAGT	29720	0.0	18.366022	9
TATGCCG	8360	0.0	17.465714	42-43
>>END_MODULE