FastQCFastQC Report
Fri 27 May 2016
SRR2138789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16389083
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA185600.043.2978321
GTATCAA327300.034.3990671
TCAACGC397450.027.491194
ATCAACG400300.027.3058383
CAACGCA405800.026.9254325
AACGCAG416000.026.4149846
TATCAAC424700.026.0895582
ACGCAGA484450.022.6226927
CGCAGAG488100.022.4535228
GTACATG459700.020.7786081
TACATGG467450.020.0165462
ACATGGG468750.019.3493563
GCAGAGT582850.018.6323999
AGAGTAC522250.018.13190310-11
CATGGGG331400.017.7445874
GAGTACT319200.017.03192512-13
CAGAGTA565650.016.43967810-11
AGTACTT358300.015.85719212-13
ATGGGGG208550.014.6761175
GTACTTT370000.014.65516114-15
Produced by FastQC (version 0.11.5)