FastQCFastQC Report
Fri 27 May 2016
SRR2138788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20482826
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA219100.045.6957631
GTATCAA366800.033.2912141
TCAACGC440250.026.8327314
ATCAACG443750.026.6305333
CAACGCA450750.026.198385
AACGCAG466100.025.3985066
TATCAAC478850.024.976242
ACGCAGA545850.021.6877237
CGCAGAG552600.021.4455498
GTACATG542250.020.2328191
TACATGG542850.019.7011412
ACATGGG544100.018.8087463
AGAGTAC594950.017.62131910-11
GCAGAGT674450.017.372329
GAGTACT363350.016.98907712-13
CATGGGG370500.016.4622154
CAGAGTA655050.016.36912510-11
AGTACTT412200.014.92995912-13
GTACTTT424300.014.63841414-15
TATACTG120850.014.0732545
Produced by FastQC (version 0.11.5)