##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138788_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20482826
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.227158254432275	33.0	31.0	34.0	30.0	34.0
2	32.353093611203846	34.0	31.0	34.0	31.0	34.0
3	32.40713478696738	34.0	31.0	34.0	30.0	34.0
4	36.031635136674986	37.0	35.0	37.0	35.0	37.0
5	35.91596330506347	37.0	35.0	37.0	35.0	37.0
6	35.93879804476199	37.0	35.0	37.0	35.0	37.0
7	35.860942332859736	37.0	35.0	37.0	35.0	37.0
8	35.82606945936073	37.0	35.0	37.0	35.0	37.0
9	37.47423461000938	39.0	37.0	39.0	35.0	39.0
10-11	37.372313468854344	39.0	37.0	39.0	34.0	39.0
12-13	37.337051025088044	39.0	37.0	39.0	34.0	39.0
14-15	38.54695189521212	40.0	38.0	41.0	34.0	41.0
16-17	38.568166692428086	40.0	38.0	41.0	34.0	41.0
18-19	38.5675370673949	40.0	38.0	41.0	34.0	41.0
20-21	38.53904531589457	40.0	38.0	41.0	34.0	41.0
22-23	38.441247890284686	40.0	38.0	41.0	34.0	41.0
24-25	38.3570499234568	40.0	38.0	41.0	34.0	41.0
26-27	38.20144046747387	40.0	38.0	41.0	33.5	41.0
28-29	38.07494351318755	40.0	37.5	41.0	33.0	41.0
30-31	37.92636262615649	40.0	37.0	41.0	33.0	41.0
32-33	38.10661612007051	40.0	37.5	41.0	33.0	41.0
34-35	38.09072340910285	40.0	37.5	41.0	33.0	41.0
36-37	38.010341844616434	40.0	37.0	41.0	33.0	41.0
38-39	37.908341654236494	40.0	37.0	41.0	33.0	41.0
40-41	37.85686519812895	40.0	37.0	41.0	33.0	41.0
42-43	37.74795993711645	40.0	37.0	41.0	33.0	41.0
44-45	37.79064567423303	40.0	37.0	41.0	33.0	41.0
46-47	37.930471181666476	40.0	37.0	41.0	33.0	41.0
48-49	37.85848381766843	40.0	37.0	41.0	33.0	41.0
50-51	37.7446956255782	40.0	37.0	41.0	32.5	41.0
52-53	37.59466777655794	40.0	36.5	41.0	32.0	41.0
54-55	37.48866742642228	39.0	36.0	41.0	32.0	41.0
56-57	37.32731631992068	39.0	36.0	41.0	32.0	41.0
58-59	37.18202929429967	39.0	35.5	40.5	31.0	41.0
60-61	36.953717540089656	39.0	35.0	40.0	31.0	41.0
62-63	36.69234454960804	38.0	35.0	40.0	31.0	41.0
64-65	36.4690364599645	38.0	35.0	40.0	31.0	41.0
66-67	36.17590148225896	37.5	34.5	40.0	30.0	41.0
68-69	35.85333932949453	37.0	34.0	39.5	30.0	41.0
70-71	35.54784958155061	36.5	34.0	39.0	30.0	41.0
72-73	35.23342460158324	36.0	34.0	39.0	29.0	40.0
74-75	34.83557623866672	35.5	34.0	38.5	29.0	40.0
76-77	33.76230102383505	35.0	32.0	37.0	28.0	39.0
78-79	34.090453456026594	35.0	33.0	37.0	29.0	39.0
80-81	33.95276653317441	35.0	33.0	36.5	29.0	39.0
82-83	33.70699037163929	35.0	33.0	36.0	29.0	38.5
84-85	33.46501566517664	35.0	33.0	36.0	29.0	37.0
86-87	33.23389468191928	35.0	32.5	35.0	29.0	37.0
88-89	32.98629979195941	34.0	32.0	35.0	28.0	36.0
90-91	32.824672247719256	34.0	32.0	35.0	28.0	36.0
92-93	32.7157326244532	34.0	32.0	35.0	27.5	36.0
94-95	32.64637613710926	34.0	32.0	35.0	28.0	35.5
96-97	32.56741213437071	34.0	32.0	35.0	27.5	35.0
98-99	32.683993909156925	34.0	32.0	35.0	29.0	35.0
100-101	31.791928307718734	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	0.0
15	1.0
16	1.0
17	2.0
18	6.0
19	17.0
20	103.0
21	567.0
22	2810.0
23	9917.0
24	26365.0
25	56649.0
26	104068.0
27	168414.0
28	247222.0
29	338757.0
30	443587.0
31	567065.0
32	723362.0
33	935590.0
34	1261867.0
35	1749755.0
36	2654920.0
37	4204544.0
38	4661253.0
39	2234733.0
40	91249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.400541019095705	22.11980417155328	14.986940766864883	24.49271404248613
2	13.906184753998483	22.86709333498417	41.25113749114674	21.975584419870607
3	16.723346671011118	28.05623110795356	31.712279350515406	23.50814287051992
4	11.926882488555982	19.608559473984798	39.99071609218174	28.473841945277478
5	12.382319705298478	38.60265668418996	36.5645736579513	12.450449952560257
6	28.35243535242647	40.00275645557893	19.262810707858378	12.381997484136221
7	24.85604281362347	33.19005883270209	24.995501109075477	16.958397244598963
8	23.389560600671018	37.75947713464929	22.0503459825319	16.800616282147786
9	25.212282719191194	16.429041578539994	21.871469298230625	36.48720640403819
10-11	23.366424144793303	27.259619839567062	30.250369260569805	19.123586755069834
12-13	24.252278469777558	24.905672195819072	30.023852665642913	20.818196668760454
14-15	21.54390463503425	26.200012634975273	27.349853970345695	24.90622875964479
16-17	21.18819764411428	29.173680904951958	28.033180406664375	21.60494104426938
18-19	21.300832707361767	28.580746133370464	29.397259440665074	20.721161718602694
20-21	22.118864395230258	27.860618459435187	29.04012545278028	20.98039169255427
22-23	21.776489040955926	27.781507839171404	28.9668079874502	21.47519513242247
24-25	21.57516206119669	27.91745318381327	29.013147066537275	21.494237688452763
26-27	21.584329598881652	28.039913421135935	28.903183416359056	21.472573563623353
28-29	21.55110809058417	28.085444199977704	28.91788101633438	21.445566693103746
30-31	21.796975534222756	27.949762327891893	28.832142844210583	21.421119293674764
32-33	21.59293588172125	28.14207114172208	28.78373906578687	21.481253910769794
34-35	21.679863565879266	28.11517029905371	28.81913916191734	21.385826973149683
36-37	21.71542926460156	28.062874678774346	28.783923171381865	21.43777288524223
38-39	21.534732985235298	28.138780985182304	28.874435064507686	21.452050965074708
40-41	21.73451832610893	28.015206516866463	28.750221451632292	21.500053705392315
42-43	21.63831607076334	28.139738406872805	28.567229405368867	21.654716116994987
44-45	21.68610920588043	28.19545291106305	28.458006868629145	21.660431014427378
46-47	21.739870137354572	28.150646650892064	28.276468735483668	21.8330144762697
48-49	21.69979998748326	28.222124105518105	28.266552803580602	21.811523103418036
50-51	21.686204922558986	28.333718612012614	28.15810258166537	21.821973883763025
52-53	21.80208447304993	28.222546683676992	28.077742038625903	21.897626804647174
54-55	21.78314969870011	28.12670599486293	28.120490808945558	21.969653497491397
56-57	21.817063919079093	28.19178507041986	28.119573096507704	21.871577913993338
58-59	21.76327961748421	28.218863498777974	28.10106736588297	21.91678951785485
60-61	21.853071499195938	28.18538146450567	28.052413607912374	21.909133428386017
62-63	21.774606303115977	28.282116277883784	28.03331612385307	21.909961295147166
64-65	21.754655218323073	28.241813096561184	28.02810238916945	21.97542929594629
66-67	21.86063071556764	28.33278010813323	27.835719902961742	21.97086927333739
68-69	21.812717364123234	28.33091965015477	27.862044742628534	21.994318243093463
70-71	21.85804096933658	28.070247374023694	27.926185582445385	22.14552607419434
72-73	21.86664778193451	28.106622746808224	27.882284470700842	22.144445000556427
74-75	21.76185469877239	28.217757609972438	27.872564099081593	22.14782359217358
76-77	21.94646015056599	28.19264198727672	27.77441327899891	22.086484583158388
78-79	21.910978625715575	28.180990040579257	27.738936347464527	22.16909498624064
80-81	21.86865677006814	28.193911526284115	27.759347975885472	22.178083727762264
82-83	21.914160017121194	28.116025529346857	27.846795184336536	22.123019269195414
84-85	21.98175638381877	28.132980388433598	27.860977570655514	22.024285657092115
86-87	21.834459872658115	28.124006912332355	27.93796889869702	22.10356431631251
88-89	21.90089237678955	28.170295876301925	27.883612640158752	22.045199106749777
90-91	21.935403159622382	28.113648793849226	27.985797819767654	21.965150226760734
92-93	21.962741936361084	28.02839851129036	27.97277472553834	22.036084826810214
94-95	21.757592778960998	28.054168430862326	28.082083502660655	22.106155287516017
96-97	21.68886203262794	28.10010390871365	28.20685071893631	22.004183339722097
98-99	21.801310845592102	28.691086433902875	28.317914129159	21.189688591346023
100-101	21.44661439905533	31.81866112625919	25.847460793212225	20.887263681473257
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3482.0
1	3285.0
2	4977.5
3	7268.5
4	9754.5
5	15021.0
6	19262.0
7	22296.5
8	24283.0
9	24164.5
10	22798.0
11	21611.5
12	24016.5
13	31262.5
14	47572.0
15	69800.5
16	87177.5
17	96034.5
18	98282.5
19	96370.5
20	92835.5
21	91589.0
22	96374.5
23	111182.0
24	137543.0
25	174274.5
26	220715.0
27	271232.0
28	323290.5
29	374362.5
30	418815.0
31	464437.5
32	513528.0
33	562794.0
34	612181.0
35	656552.5
36	702730.0
37	744460.5
38	772581.0
39	794408.0
40	818925.5
41	842691.5
42	857455.0
43	869607.5
44	878287.0
45	880246.0
46	875074.0
47	865264.5
48	849764.5
49	828017.0
50	802439.0
51	766594.0
52	720580.0
53	671236.5
54	620029.0
55	568280.5
56	517217.5
57	464274.0
58	409485.5
59	352768.5
60	295779.0
61	240524.0
62	190015.0
63	146734.5
64	109907.0
65	82725.0
66	62855.5
67	47946.0
68	36507.5
69	26363.0
70	19077.0
71	13913.5
72	9791.0
73	6876.5
74	4820.5
75	3391.5
76	2351.5
77	1615.5
78	1118.0
79	802.5
80	585.0
81	420.0
82	301.5
83	212.0
84	153.5
85	115.0
86	92.5
87	79.0
88	59.0
89	40.0
90	26.5
91	18.0
92	15.0
93	10.0
94	4.5
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001215652566691725
3	0.0
4	3.5151399518796873E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0033100901213533717
18-19	0.0
20-21	0.002209245798636986
22-23	0.0
24-25	2.783835604837369E-4
26-27	0.003534773141459887
28-29	0.0
30-31	2.0307122473650226E-4
32-33	0.0010031287561377562
34-35	7.676943962147549E-4
36-37	0.010033991196050515
38-39	0.011647785990681423
40-41	0.024968465209714664
42-43	0.002017726307158577
44-45	0.0013728189753182203
46-47	0.001799783474187018
48-49	0.0016414274186328794
50-51	6.05218276181519E-5
52-53	1.035702552494252E-4
54-55	0.006129083535390395
56-57	0.0036216927440264925
58-59	0.003770995529976668
60-61	0.001613346397019022
62-63	4.273026986343825E-4
64-65	7.73520572739475E-4
66-67	6.16859190038181E-4
68-69	1.4456364648729711E-5
70-71	0.0
72-73	1.39439857631312E-4
74-75	3.03485713710508E-4
76-77	0.001433645542371394
78-79	0.0018511247711778393
80-81	0.011020449477172031
82-83	0.009563483701554472
84-85	0.01268974662988683
86-87	0.006134616678984484
88-89	0.08737705783496551
90-91	3.8341572926044946E-4
92-93	5.196215686183492E-5
94-95	2.65958437079287E-4
96-97	0.014422472318196308
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	450.0
20-21	1607.0
22-23	3457.0
24-25	7114.0
26-27	8468.0
28-29	14813.0
30-31	47071.0
32-33	60371.0
34-35	84556.0
36-37	135511.0
38-39	149091.0
40-41	154055.0
42-43	172992.0
44-45	199981.0
46-47	203691.0
48-49	192142.0
50-51	179551.0
52-53	159578.0
54-55	176469.0
56-57	185149.0
58-59	197612.0
60-61	199211.0
62-63	189859.0
64-65	198973.0
66-67	213094.0
68-69	220332.0
70-71	221348.0
72-73	213479.0
74-75	213567.0
76-77	215076.0
78-79	233650.0
80-81	240452.0
82-83	232240.0
84-85	231407.0
86-87	250042.0
88-89	268254.0
90-91	298095.0
92-93	318799.0
94-95	404373.0
96-97	527550.0
98-99	1751149.0
100-101	1.1508147E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.99840410955895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.70882958485558	73.62691285969841
2	7.566326744658433	12.559860895562483
3	1.9968344377838378	4.972022148212006
4	0.769027438309837	2.5531220038471503
5	0.3656117516587887	1.5172595955689923
6	0.19469610874821575	0.9695679787465825
7	0.1169697555734159	0.6795812129175094
8	0.07760597059575881	0.515293736705707
9	0.048447850296874415	0.36189848314614576
>10	0.15232567950085021	1.9512896865782836
>50	0.002628162916539711	0.14240703102305788
>100	6.428096991033355E-4	0.08472015972586054
>500	1.0765950886202183E-5	0.005799183257375608
>1k	4.293945193734497E-5	0.06026502501033482
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.8821388220551205E-6	4.8821388220551205E-6
2	0.0	2.9292832932330723E-5	0.0	1.4646416466165361E-5	4.8821388220551205E-6
3	0.0	3.4174971754385843E-5	0.0	1.9528555288220482E-5	4.8821388220551205E-6
4	0.0	7.811422115288193E-5	0.0	3.4174971754385843E-5	4.8821388220551205E-6
5	0.0	8.299635997493706E-5	0.0	3.9057110576440964E-5	4.8821388220551205E-6
6	0.0	8.299635997493706E-5	0.0	3.9057110576440964E-5	9.764277644110241E-6
7	4.8821388220551205E-6	8.299635997493706E-5	0.0	3.9057110576440964E-5	9.764277644110241E-6
8	9.764277644110241E-6	1.0740705408521265E-4	0.0	4.8821388220551205E-5	9.764277644110241E-6
9	9.764277644110241E-6	1.5622844230576386E-4	0.0	6.834994350877169E-5	9.764277644110241E-6
10-11	9.764277644110241E-6	1.5622844230576386E-4	2.4410694110275602E-6	1.1228919290726778E-4	9.764277644110241E-6
12-13	1.4646416466165361E-5	1.5622844230576386E-4	4.8821388220551205E-6	1.3181774819548826E-4	9.764277644110241E-6
14-15	1.4646416466165361E-5	2.2457838581453556E-4	9.764277644110241E-6	1.3914095642857094E-4	9.764277644110241E-6
16-17	1.4646416466165361E-5	2.3922480228070092E-4	9.764277644110241E-6	1.733159281829568E-4	9.764277644110241E-6
18-19	1.4646416466165361E-5	2.709587046240592E-4	9.764277644110241E-6	2.2457838581453556E-4	9.764277644110241E-6
20-21	1.4646416466165361E-5	3.075747457894726E-4	9.764277644110241E-6	3.0025153755638994E-4	9.764277644110241E-6
22-23	1.9528555288220482E-5	3.9301217517543724E-4	9.764277644110241E-6	5.150656457268152E-4	9.764277644110241E-6
24-25	2.6851763521303163E-5	4.247460775187955E-4	9.764277644110241E-6	0.0012644739549122763	1.2205347055137801E-5
26-27	2.9292832932330723E-5	4.393924939849609E-4	9.764277644110241E-6	0.003393086481328309	1.4646416466165361E-5
28-29	3.4174971754385843E-5	4.393924939849609E-4	9.764277644110241E-6	0.01804926722513778	1.4646416466165361E-5
30-31	3.4174971754385843E-5	4.7844960456140185E-4	9.764277644110241E-6	0.029695609385150274	1.4646416466165361E-5
32-33	3.4174971754385843E-5	4.906549516165397E-4	9.764277644110241E-6	0.044193120617242954	2.4410694110275602E-5
34-35	3.661604116541341E-5	5.809745198245594E-4	9.764277644110241E-6	0.06361670992078924	2.4410694110275602E-5
36-37	3.9057110576440964E-5	6.688530186215515E-4	1.2205347055137801E-5	0.08927723156951096	2.4410694110275602E-5
38-39	4.3939249398496084E-5	6.88381573909772E-4	1.4646416466165361E-5	0.13068997412759353	2.4410694110275602E-5
40-41	4.638031880952365E-5	7.27438684486213E-4	1.4646416466165361E-5	0.19140425251867102	2.4410694110275602E-5
42-43	5.3703527042606325E-5	7.616136562405988E-4	1.4646416466165361E-5	0.2261162595434829	2.4410694110275602E-5
44-45	5.3703527042606325E-5	8.690207103258115E-4	1.4646416466165361E-5	0.2753208956615655	2.4410694110275602E-5
46-47	5.3703527042606325E-5	9.422527926566383E-4	1.4646416466165361E-5	0.3303181894920164	2.4410694110275602E-5
48-49	5.3703527042606325E-5	9.49576000889721E-4	1.4646416466165361E-5	0.3732004558355375	2.4410694110275602E-5
50-51	5.8585665864661446E-5	0.0010179259443984928	2.9292832932330723E-5	0.4148060428770913	2.4410694110275602E-5
52-53	6.102673527568901E-5	0.0010252491526315753	2.9292832932330723E-5	0.4565385655280184	2.9292832932330723E-5
54-55	6.83499435087717E-5	0.0010472187773308233	2.9292832932330723E-5	0.5471242103018401	3.9057110576440964E-5
56-57	7.323208233082681E-5	0.0010691884020300713	3.173390234335828E-5	0.6237933183633938	4.3939249398496084E-5
58-59	7.323208233082681E-5	0.001071629471441099	3.4174971754385843E-5	0.687580903142955	4.3939249398496084E-5
60-61	7.811422115288193E-5	0.0010960401655513746	3.4174971754385843E-5	0.7215410607891704	4.3939249398496084E-5
62-63	7.811422115288193E-5	0.0011350972761278156	3.4174971754385843E-5	0.7414772746690325	4.3939249398496084E-5
64-65	8.05552905639095E-5	0.0013059721348997449	3.4174971754385843E-5	0.7558307628058746	4.3939249398496084E-5
66-67	8.787849879699217E-5	0.0013108542737217998	3.4174971754385843E-5	0.7644184449938696	4.3939249398496084E-5
68-69	9.031956820801973E-5	0.0013499113842982409	3.4174971754385843E-5	0.772166399304471	4.3939249398496084E-5
70-71	9.520170703007485E-5	0.0013816452866415993	3.4174971754385843E-5	0.7795506342728293	4.3939249398496084E-5
72-73	9.764277644110241E-5	0.001403614911340847	3.4174971754385843E-5	0.7857729202015387	4.8821388220551205E-5
74-75	1.0008384585212998E-4	0.0014304666748621503	3.4174971754385843E-5	0.7914239958880674	4.8821388220551205E-5
76-77	1.1228919290726778E-4	0.0015110219654260598	3.4174971754385843E-5	0.796345191820699	4.8821388220551205E-5
78-79	1.1717133172932289E-4	0.0015427558677694182	3.4174971754385843E-5	0.8009563719381301	4.8821388220551205E-5
80-81	1.1717133172932289E-4	0.0015427558677694182	3.661604116541341E-5	0.803526818027942	4.8821388220551205E-5
82-83	1.1717133172932289E-4	0.0015476380065914733	3.9057110576440964E-5	0.8052477719627165	4.8821388220551205E-5
84-85	1.2693560937343315E-4	0.0015574022842355835	3.9057110576440964E-5	0.8067002082622778	4.8821388220551205E-5
86-87	1.2693560937343315E-4	0.0015964593948120244	3.9057110576440964E-5	0.806890611676338	4.8821388220551205E-5
88-89	1.2937667878446072E-4	0.001659927199498741	3.9057110576440964E-5	0.8069589616198468	4.8821388220551205E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21910	0.0	45.695763	1
GTATCAA	36680	0.0	33.291214	1
TCAACGC	44025	0.0	26.832731	4
ATCAACG	44375	0.0	26.630533	3
CAACGCA	45075	0.0	26.19838	5
AACGCAG	46610	0.0	25.398506	6
TATCAAC	47885	0.0	24.97624	2
ACGCAGA	54585	0.0	21.687723	7
CGCAGAG	55260	0.0	21.445549	8
GTACATG	54225	0.0	20.232819	1
TACATGG	54285	0.0	19.701141	2
ACATGGG	54410	0.0	18.808746	3
AGAGTAC	59495	0.0	17.621319	10-11
GCAGAGT	67445	0.0	17.37232	9
GAGTACT	36335	0.0	16.989077	12-13
CATGGGG	37050	0.0	16.462215	4
CAGAGTA	65505	0.0	16.369125	10-11
AGTACTT	41220	0.0	14.929959	12-13
GTACTTT	42430	0.0	14.638414	14-15
TATACTG	12085	0.0	14.073254	5
>>END_MODULE