FastQCFastQC Report
Fri 27 May 2016
SRR2138787_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138787_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17879274
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA220650.048.3337481
GTATCAA396550.034.5109181
ATCAACG494250.026.9927923
TCAACGC495700.026.8971164
CAACGCA505100.026.3880585
TATCAAC522750.025.7276652
AACGCAG521200.025.6683166
ACCGAGG198500.024.41023692-93
GACCGAG197750.024.18046690-91
GAGACCG200100.023.63763888-89
AGACCGA197750.023.60013490-91
CGAGACC199350.023.41621688-89
ACGAGAC212750.022.49985586-87
ACGCAGA602250.022.1519957
CGCAGAG608400.021.941698
GCCCACG213400.021.67192682-83
CCCACGA212850.021.59531684-85
CACGAGA218600.021.33129186-87
CCACGAG223000.021.0895984-85
GTACATG637900.020.8106581
Produced by FastQC (version 0.11.5)