##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138787_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17879274
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19224757112621	33.0	31.0	34.0	30.0	34.0
2	32.315923621954674	34.0	31.0	34.0	30.0	34.0
3	32.369755953177965	34.0	31.0	34.0	30.0	34.0
4	36.00121179417017	37.0	35.0	37.0	35.0	37.0
5	35.88699250316316	37.0	35.0	37.0	35.0	37.0
6	35.913796276068034	37.0	35.0	37.0	35.0	37.0
7	35.834652905928955	37.0	35.0	37.0	35.0	37.0
8	35.799568763250676	37.0	35.0	37.0	33.0	37.0
9	37.438594318762604	39.0	37.0	39.0	34.0	39.0
10-11	37.33639626530697	39.0	37.0	39.0	34.0	39.0
12-13	37.29902545259947	39.0	37.0	39.0	34.0	39.0
14-15	38.4832228366767	40.0	38.0	41.0	34.0	41.0
16-17	38.50968903994648	40.0	38.0	41.0	34.0	41.0
18-19	38.5270311870605	40.0	38.0	41.0	34.0	41.0
20-21	38.50469992668204	40.0	38.0	41.0	34.0	41.0
22-23	38.4069369528826	40.0	38.0	41.0	34.0	41.0
24-25	38.32070784277	40.0	38.0	41.0	34.0	41.0
26-27	38.16225043769485	40.0	38.0	41.0	33.0	41.0
28-29	38.03545685967507	40.0	37.0	41.0	33.0	41.0
30-31	37.88197193328885	40.0	37.0	41.0	33.0	41.0
32-33	38.05599311176671	40.0	37.0	41.0	33.0	41.0
34-35	38.038541955128736	40.0	37.0	41.0	33.0	41.0
36-37	37.95987200802103	40.0	37.0	41.0	33.0	41.0
38-39	37.86140988438698	40.0	37.0	41.0	33.0	41.0
40-41	37.81817831110682	40.0	37.0	41.0	33.0	41.0
42-43	37.7188924787953	40.0	37.0	41.0	33.0	41.0
44-45	37.764338022644935	40.0	37.0	41.0	33.0	41.0
46-47	37.90374741106511	40.0	37.0	41.0	33.0	41.0
48-49	37.83947964452324	40.0	37.0	41.0	33.0	41.0
50-51	37.73225999890998	40.0	37.0	41.0	32.5	41.0
52-53	37.59611239404137	40.0	36.5	41.0	32.0	41.0
54-55	37.5068236040555	39.5	36.0	41.0	32.0	41.0
56-57	37.35686345328861	39.0	36.0	41.0	32.0	41.0
58-59	37.22610887637093	39.0	36.0	41.0	31.0	41.0
60-61	37.01701647576108	39.0	35.0	40.0	31.0	41.0
62-63	36.77109215343427	38.5	35.0	40.0	31.0	41.0
64-65	36.570738923087426	38.0	35.0	40.0	31.0	41.0
66-67	36.29700105219442	38.0	35.0	40.0	30.5	41.0
68-69	35.99224451085226	37.0	34.0	40.0	30.0	41.0
70-71	35.70192815670299	37.0	34.0	39.0	30.0	41.0
72-73	35.39411687231705	36.0	34.0	39.0	29.0	40.5
74-75	35.006662981933886	36.0	34.0	39.0	29.0	40.0
76-77	33.92187781426671	35.0	32.5	37.0	28.0	39.0
78-79	34.25684283771125	35.0	33.0	37.0	29.0	39.0
80-81	34.10031532580256	35.0	33.0	37.0	29.0	39.0
82-83	33.8331534087811	35.0	33.0	36.0	29.0	38.5
84-85	33.58284085345156	35.0	33.0	36.0	29.0	37.0
86-87	33.340887147313694	35.0	33.0	35.5	29.0	37.0
88-89	33.08223061362803	34.5	32.0	35.0	29.0	36.5
90-91	32.90538515255794	34.0	32.0	35.0	28.0	36.0
92-93	32.793148492327845	34.0	32.0	35.0	28.5	36.0
94-95	32.708152372698066	34.0	32.0	35.0	28.5	36.0
96-97	32.62311769951978	34.0	32.0	35.0	28.0	35.0
98-99	32.73446963316669	34.0	32.0	35.0	29.0	35.0
100-101	31.837081692994662	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	2.0
18	5.0
19	8.0
20	89.0
21	506.0
22	2381.0
23	8691.0
24	22877.0
25	49292.0
26	90605.0
27	146164.0
28	216017.0
29	294680.0
30	387612.0
31	493738.0
32	628839.0
33	811074.0
34	1088891.0
35	1492550.0
36	2236854.0
37	3564428.0
38	4174820.0
39	2090206.0
40	78943.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.295760778653545	22.631769052815006	15.097262897811175	23.975207270720276
2	13.547232420686239	23.193514600940297	41.79209929115936	21.4671536872141
3	16.458380804500226	28.852664822967643	31.953803045917862	22.735151326614268
4	11.421527337322322	20.03452052158875	40.63543599776724	27.908516143321688
5	11.877434173222023	39.25222578948116	36.7038169446925	12.16652309260432
6	27.346306119588526	40.64978812898107	19.768940282474556	12.234965468955842
7	24.267232550941387	33.639167899099256	25.34651015471881	16.747089395240543
8	23.193111756103743	37.96806850211032	22.2998484166639	16.538971325122038
9	25.191095566855793	16.329494139415278	22.178059355206482	36.30135093852245
10-11	22.960345034144	27.669624616748976	30.487823498873613	18.882206850233406
12-13	23.98210632042442	25.443684122744582	30.329122983405256	20.245086573425745
14-15	21.211898760542514	26.6448095151962	27.868502938094693	24.2747887861666
16-17	20.62483856457747	29.666240199352682	28.508919513346243	21.20000172272361
18-19	20.680814556564208	28.96879929240975	30.080337713936263	20.270048437089784
20-21	21.675609002490848	28.160789992220714	29.708203576054302	20.455397429234136
22-23	21.308683953173524	28.217344188525356	29.573259610437574	20.90071224786354
24-25	21.012848412810293	28.320775382553965	29.699961804821097	20.966414399814646
26-27	21.021234906065235	28.44387493082352	29.580680950144405	20.95420921296684
28-29	20.95385461996006	28.491641087711788	29.60607872836781	20.948425563960342
30-31	21.20011698753946	28.386271727558988	29.540284499969665	20.87332678493189
32-33	21.049769677365575	28.476457696779278	29.509854373346865	20.963918252508275
34-35	21.04518360907409	28.53535785298984	29.603227981861846	20.816230556074228
36-37	21.090093375816156	28.554830915626784	29.44386205444044	20.911213654116622
38-39	20.95031125385849	28.550855474595128	29.526836784296883	20.9719964872495
40-41	21.09984354502627	28.435980555026013	29.447942063590265	21.016233836357443
42-43	21.05710525874837	28.574912797922973	29.242743516512775	21.125238426815883
44-45	21.114627972354537	28.695968016718666	29.03324805206934	21.156155958857457
46-47	21.203398181598548	28.64141168601721	28.804349572475125	21.35084055990912
48-49	21.175943013704437	28.735155152021925	28.746394714027666	21.342507120245973
50-51	21.188051412213355	28.82749157712496	28.60350991395445	21.38094709670724
52-53	21.227405006693857	28.74997753946224	28.595695182565407	21.4269222712785
54-55	21.22974290698069	28.736528150075745	28.561452661489156	21.472276281454405
56-57	21.310452056627096	28.739217888853542	28.537411071394896	21.412918983124463
58-59	21.220779217480125	28.79107446922284	28.583064971539716	21.405081341757317
60-61	21.222626483421873	28.65139197369783	28.612214064920433	21.513767477959867
62-63	21.400278975434755	28.698351990683076	28.57699116797981	21.324377865902363
64-65	21.056384832559765	28.691615394059973	28.602468248087536	21.649531525292726
66-67	21.212372903071362	28.705280866994254	28.432092010359987	21.650254219574393
68-69	21.177938043073873	28.95730892237825	28.45329923695474	21.411453797593133
70-71	21.189378780718393	28.74436811061372	28.523374355250148	21.542878753417742
72-73	21.22688121763647	28.725044719254388	28.27099383960279	21.77708022350635
74-75	21.183179771971897	28.761320767947048	28.232512235825375	21.822987224255684
76-77	21.273203490978197	28.492225708297266	28.466070667727983	21.76850013299655
78-79	21.28929296680298	28.437518195674137	28.417486955447973	21.855701882074907
80-81	21.549582181761924	28.44122795605158	28.13903202950836	21.87015783267814
82-83	21.57146408780448	28.686052503285797	28.236897173263927	21.505586235645797
84-85	21.37603904510696	28.447954729697027	28.154091750556574	22.021914474639434
86-87	21.24776189445915	28.718516700738938	28.240516363909045	21.793205040892868
88-89	21.626800836098177	28.33753365903903	28.215218953885145	21.82044655097765
90-91	21.68253273346375	28.619870765746285	28.282094439570127	21.415502061219836
92-93	21.340192659886988	28.525485037602266	28.327265273333897	21.807057029176853
94-95	21.4920035114857	28.26442324898521	28.435250704745634	21.808322534783457
96-97	21.393769977502348	28.63164503066991	28.557110404146606	21.41747458768113
98-99	21.562250212609403	29.012711075523246	28.73237491546356	20.692663796403792
100-101	23.887383366540494	36.72709934864551	30.115616211545156	9.269901073268844
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3379.0
1	3333.0
2	5346.5
3	7902.5
4	10822.5
5	16928.5
6	21604.0
7	24874.0
8	26776.0
9	25912.5
10	23918.0
11	22400.5
12	25328.5
13	34092.0
14	53711.5
15	80113.5
16	100012.5
17	108927.5
18	109177.5
19	104813.0
20	99360.5
21	96763.0
22	101218.0
23	116202.0
24	142237.5
25	177803.0
26	222261.0
27	270556.5
28	320989.5
29	372682.0
30	419586.0
31	464033.0
32	508776.0
33	551238.5
34	591647.0
35	629740.0
36	666338.0
37	697915.0
38	719899.0
39	735583.5
40	749111.0
41	757454.0
42	758135.0
43	758337.5
44	757416.5
45	751997.0
46	741617.0
47	728271.5
48	713722.5
49	693272.5
50	668949.5
51	635014.5
52	589009.5
53	544201.5
54	500987.0
55	454528.0
56	407981.0
57	362038.0
58	315301.0
59	268284.0
60	222487.0
61	178871.0
62	139174.5
63	105972.5
64	78842.0
65	58460.5
66	43466.5
67	32293.0
68	23829.5
69	16741.5
70	11619.5
71	8214.0
72	5807.0
73	4125.5
74	2957.5
75	2112.5
76	1486.0
77	1000.0
78	693.5
79	506.0
80	367.5
81	267.5
82	207.5
83	153.0
84	110.0
85	81.5
86	61.0
87	50.5
88	38.0
89	26.0
90	19.5
91	15.0
92	12.0
93	6.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0012304750181690823
3	0.0
4	3.020256862778656E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0034005855047581908
18-19	0.0
20-21	0.00213383198441872
22-23	0.0
24-25	3.608848089819921E-4
26-27	0.0035318149924482966
28-29	0.0
30-31	9.812499672332601E-5
32-33	7.984023182454693E-4
34-35	7.758860484656798E-4
36-37	0.009789283613644123
38-39	0.011405960755090611
40-41	0.02476390899254797
42-43	0.0020130311769658033
44-45	0.001311052534433575
46-47	0.0019369010018360098
48-49	0.0018215406241344858
50-51	8.222185875423592E-5
52-53	1.4460873261368438E-4
54-55	0.006362022700740765
56-57	0.0037767888290937443
58-59	0.003715054435708316
60-61	0.0016984908732664479
62-63	4.565702893198495E-4
64-65	7.667526358022955E-4
66-67	6.105705625946489E-4
68-69	1.0089542333987595E-5
70-71	0.0
72-73	9.338118646469447E-5
74-75	2.3139329687276166E-4
76-77	0.0016064531079181077
78-79	0.0019540532851583486
80-81	0.011210448240305718
82-83	0.009989744135631248
84-85	0.01298637715804005
86-87	0.0065225803819681455
88-89	0.08848975709270478
90-91	3.9451315880708785E-4
92-93	4.4791726007220344E-5
94-95	2.3775801698134496E-4
96-97	0.01538045877219626
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	383.0
20-21	1422.0
22-23	3061.0
24-25	6329.0
26-27	7980.0
28-29	15265.0
30-31	45780.0
32-33	59023.0
34-35	81995.0
36-37	129715.0
38-39	147595.0
40-41	151869.0
42-43	171682.0
44-45	200484.0
46-47	201646.0
48-49	190613.0
50-51	175152.0
52-53	152720.0
54-55	166616.0
56-57	176489.0
58-59	112009.0
60-61	195252.0
62-63	181408.0
64-65	186487.0
66-67	200701.0
68-69	204918.0
70-71	206643.0
72-73	196334.0
74-75	193905.0
76-77	194511.0
78-79	213623.0
80-81	220696.0
82-83	210356.0
84-85	206534.0
86-87	220147.0
88-89	238543.0
90-91	265006.0
92-93	279920.0
94-95	348012.0
96-97	447668.0
98-99	1539613.0
100-101	9731169.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.08114566057418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.14204714700502	74.951654506491
2	7.415867699273732	12.470693044443633
3	1.9141579150510426	4.828337714182431
4	0.7115500547653696	2.393117751980663
5	0.33041409092942897	1.389079765387176
6	0.17158768302072125	0.8656373381775417
7	0.09521821802792937	0.5604239801682647
8	0.05984324931732513	0.4025351170121656
9	0.037730202955545175	0.28551588214573986
>10	0.11733317220727084	1.513526961981489
>50	0.003145088765039798	0.17917490631155042
>100	0.0010708610177186174	0.13946845314060347
>500	3.4617663833218705E-5	0.020834578577793764
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.593068264404919E-6	0.0	0.0	0.0	5.593068264404919E-6
2	5.593068264404919E-6	2.2372273057619676E-5	0.0	5.593068264404919E-6	5.593068264404919E-6
3	5.593068264404919E-6	2.2372273057619676E-5	0.0	1.677920479321476E-5	5.593068264404919E-6
4	5.593068264404919E-6	3.355840958642952E-5	0.0	3.355840958642952E-5	5.593068264404919E-6
5	5.593068264404919E-6	3.355840958642952E-5	0.0	3.355840958642952E-5	5.593068264404919E-6
6	1.1186136528809838E-5	3.355840958642952E-5	0.0	4.474454611523935E-5	1.677920479321476E-5
7	1.1186136528809838E-5	3.355840958642952E-5	0.0	5.593068264404919E-5	2.2372273057619676E-5
8	1.1186136528809838E-5	3.355840958642952E-5	0.0	6.152375090845411E-5	2.2372273057619676E-5
9	1.1186136528809838E-5	4.474454611523935E-5	0.0	8.94890922304787E-5	5.0337614379644276E-5
10-11	3.355840958642952E-5	4.474454611523935E-5	0.0	1.1186136528809839E-4	5.0337614379644276E-5
12-13	5.0337614379644276E-5	4.474454611523935E-5	0.0	1.3143710421351562E-4	5.0337614379644276E-5
14-15	5.313414851184674E-5	8.109948983387133E-5	0.0	1.4262324074232547E-4	6.991335330506148E-5
16-17	6.152375090845411E-5	8.94890922304787E-5	0.0	1.649955137999451E-4	9.508216049488363E-5
18-19	6.711681917285903E-5	1.0906483115589592E-4	0.0	1.8457125272536233E-4	9.508216049488363E-5
20-21	6.711681917285903E-5	1.3143710421351562E-4	0.0	2.293157988406017E-4	9.508216049488363E-5
22-23	6.711681917285903E-5	1.538093772711353E-4	0.0	4.893934731354304E-4	1.1465789942030085E-4
24-25	9.228562636268117E-5	1.789781844609574E-4	0.0	0.0013339467810605733	1.3143710421351562E-4
26-27	1.0626829702369346E-4	1.9016432098976725E-4	0.0	0.0038704032389682043	1.39826706610123E-4
28-29	1.0906483115589592E-4	1.929608551219697E-4	0.0	0.020176993763840745	1.4541977487452792E-4
30-31	1.2304750181690822E-4	2.1253659404738692E-4	0.0	0.031069494208769324	1.4541977487452792E-4
32-33	1.3423363834571807E-4	2.1253659404738692E-4	0.0	0.03937799711554284	1.5660591140333774E-4
34-35	1.4541977487452792E-4	2.4609500363381644E-4	0.0	0.050085926307746054	1.6779204793214759E-4
36-37	1.5660591140333774E-4	2.8244994735244843E-4	0.0	0.06460553152214121	1.7058858206435006E-4
38-39	1.6219897966774266E-4	2.880430156168534E-4	0.0	0.09167318538772884	1.733851161965525E-4
40-41	1.6779204793214759E-4	3.243979593354853E-4	0.0	0.14051465400664478	1.789781844609574E-4
42-43	1.6779204793214759E-4	3.243979593354853E-4	0.0	0.16228846875997313	1.873677868575648E-4
44-45	1.789781844609574E-4	3.4117716412870007E-4	0.0	0.19336075950287468	1.9855392338637465E-4
46-47	1.929608551219697E-4	3.6075290305411726E-4	0.0	0.22982756458679476	2.0974005991518448E-4
48-49	2.013504575185771E-4	3.663459713185222E-4	0.0	0.2600497089535067	2.1253659404738692E-4
50-51	2.0414699165077955E-4	4.1109051743376155E-4	0.0	0.29292017114341445	2.1253659404738692E-4
52-53	2.1812966231179185E-4	4.1388705156596405E-4	0.0	0.33006373748732754	2.572811401626263E-4
54-55	2.2372273057619677E-4	4.166835856981665E-4	0.0	0.4149133795924823	3.020256862778656E-4
56-57	2.2372273057619677E-4	4.2507318809477385E-4	0.0	0.49236059584969727	3.020256862778656E-4
58-59	2.2372273057619677E-4	4.3346279049138125E-4	0.0	0.5586552339876887	3.1321182280667553E-4
60-61	2.4050193536941154E-4	4.362593246235837E-4	0.0	1.0251590752510422	3.188048910710804E-4
62-63	2.4050193536941154E-4	4.446489270201911E-4	0.0	1.0365353761008418	3.6914250545072466E-4
64-65	2.4050193536941154E-4	5.397310875150747E-4	0.0	1.0479815903039464	3.803286419795345E-4
66-67	2.4050193536941154E-4	5.509172240438845E-4	0.0	1.0568661792419536	3.971078467727493E-4
68-69	2.4609500363381644E-4	5.565102923082894E-4	0.0	1.065781530055415	4.027009150371542E-4
70-71	2.4609500363381644E-4	5.59306826440492E-4	0.0	1.0740424918819411	4.1948011983036895E-4
72-73	2.4609500363381644E-4	5.59306826440492E-4	0.0	1.0809135762447624	4.5303852941679844E-4
74-75	2.4609500363381644E-4	5.788825653659092E-4	0.0	1.0867974840589165	4.5303852941679844E-4
76-77	2.4609500363381644E-4	6.096444408201362E-4	0.0	1.0919542929986976	4.5303852941679844E-4
78-79	2.516880718982214E-4	6.20830577348946E-4	0.0	1.096610522328815	4.5303852941679844E-4
80-81	2.516880718982214E-4	6.32016713877756E-4	0.0	1.099331550039448	4.5583506354900094E-4
82-83	2.572811401626263E-4	6.376097821421609E-4	0.0	1.1009954878481083	4.921900072676329E-4
84-85	2.628742084270312E-4	6.487959186709706E-4	0.0	1.102438499460325	4.921900072676329E-4
86-87	2.628742084270312E-4	6.655751234641854E-4	0.0	1.1026678152591654	4.921900072676329E-4
88-89	2.628742084270312E-4	6.87947396521805E-4	0.0	1.102718152873545	4.921900072676329E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	22065	0.0	48.333748	1
GTATCAA	39655	0.0	34.510918	1
ATCAACG	49425	0.0	26.992792	3
TCAACGC	49570	0.0	26.897116	4
CAACGCA	50510	0.0	26.388058	5
TATCAAC	52275	0.0	25.727665	2
AACGCAG	52120	0.0	25.668316	6
ACCGAGG	19850	0.0	24.410236	92-93
GACCGAG	19775	0.0	24.180466	90-91
GAGACCG	20010	0.0	23.637638	88-89
AGACCGA	19775	0.0	23.600134	90-91
CGAGACC	19935	0.0	23.416216	88-89
ACGAGAC	21275	0.0	22.499855	86-87
ACGCAGA	60225	0.0	22.151995	7
CGCAGAG	60840	0.0	21.94169	8
GCCCACG	21340	0.0	21.671926	82-83
CCCACGA	21285	0.0	21.595316	84-85
CACGAGA	21860	0.0	21.331291	86-87
CCACGAG	22300	0.0	21.08959	84-85
GTACATG	63790	0.0	20.810658	1
>>END_MODULE