FastQCFastQC Report
Fri 27 May 2016
SRR2138786_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138786_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16078147
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172150.047.2728081
TATCTCG200650.040.5343694-95
GTATCAA298900.033.876871
CCGTCTT83600.031.0216892-93
TGCCGTC82050.030.83550690-91
TATGCCG80600.030.6249188-89
GCCGTCT85100.029.04731690-91
ATGCCGT84300.028.32939588-89
CTATCTC276200.028.08591894-95
TCAACGC363500.026.9800324
ATCAACG365250.026.885653
CAACGCA371550.026.4296975
CGTATGC95450.026.2639986-87
AACGCAG376600.026.1091256
ATCTCGT157500.025.84698194-95
TATCAAC393000.025.2358112
CGCTATC292650.025.11033692-93
GCTATCT343900.025.01574794-95
CTCGTAT98950.024.7132484-85
TCGTATG96950.024.6357984-85
Produced by FastQC (version 0.11.5)