##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138786_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16078147
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23920138309471	33.0	31.0	34.0	30.0	34.0
2	32.36592357315803	34.0	31.0	34.0	31.0	34.0
3	32.422393078008305	34.0	31.0	34.0	31.0	34.0
4	36.040686840342985	37.0	35.0	37.0	35.0	37.0
5	35.92249511091048	37.0	35.0	37.0	35.0	37.0
6	35.94623708814206	37.0	35.0	37.0	35.0	37.0
7	35.86706938305764	37.0	35.0	37.0	35.0	37.0
8	35.83179492014845	37.0	35.0	37.0	35.0	37.0
9	37.4848095990166	39.0	37.0	39.0	35.0	39.0
10-11	37.38156240890197	39.0	37.0	39.0	34.0	39.0
12-13	37.34418223070109	39.0	37.0	39.0	34.0	39.0
14-15	38.55579657282646	40.0	38.0	41.0	34.0	41.0
16-17	38.57555743208468	40.0	38.0	41.0	34.0	41.0
18-19	38.57725035105103	40.0	38.0	41.0	34.0	41.0
20-21	38.54805018518117	40.0	38.0	41.0	34.0	41.0
22-23	38.448819552673	40.0	38.0	41.0	34.0	41.0
24-25	38.36225176310161	40.0	38.0	41.0	34.0	41.0
26-27	38.204393251117565	40.0	38.0	41.0	33.5	41.0
28-29	38.07692119171439	40.0	37.5	41.0	33.0	41.0
30-31	37.92546782769067	40.0	37.0	41.0	33.0	41.0
32-33	38.11232676618668	40.0	37.5	41.0	33.0	41.0
34-35	38.09772355976459	40.0	37.5	41.0	33.0	41.0
36-37	38.017968007125475	40.0	37.0	41.0	33.0	41.0
38-39	37.919668635056084	40.0	37.0	41.0	33.0	41.0
40-41	37.87159606799021	40.0	37.0	41.0	33.0	41.0
42-43	37.764846241833865	40.0	37.0	41.0	33.0	41.0
44-45	37.80422509227359	40.0	37.0	41.0	33.0	41.0
46-47	37.93879797845402	40.0	37.0	41.0	33.0	41.0
48-49	37.86385725804482	40.0	37.0	41.0	33.0	41.0
50-51	37.74866117869382	40.0	37.0	41.0	32.5	41.0
52-53	37.59683172872049	40.0	36.5	41.0	32.0	41.0
54-55	37.499836311967215	39.0	36.0	41.0	32.0	41.0
56-57	37.33961163913927	39.0	36.0	41.0	32.0	41.0
58-59	37.20074291293186	39.0	35.5	41.0	31.0	41.0
60-61	36.979146517753705	39.0	35.0	40.0	31.0	41.0
62-63	36.72084046900315	38.0	35.0	40.0	31.0	41.0
64-65	36.51599899223373	38.0	35.0	40.0	31.0	41.0
66-67	36.22754982848868	37.5	34.5	40.0	30.5	41.0
68-69	35.90547181949589	37.0	34.0	40.0	30.0	41.0
70-71	35.5976738829733	36.5	34.0	39.0	30.0	41.0
72-73	35.28759993613869	36.0	34.0	39.0	29.5	40.0
74-75	34.881338606913275	35.5	34.0	38.5	29.0	40.0
76-77	33.79460990115655	35.0	32.5	37.0	28.0	39.0
78-79	34.089526927497616	35.0	33.0	37.0	29.0	39.0
80-81	33.923194799137725	35.0	33.0	36.5	29.0	39.0
82-83	33.67318789751532	35.0	33.0	36.0	29.0	37.5
84-85	33.456198167285265	35.0	33.0	36.0	29.0	37.0
86-87	33.23929377961352	35.0	33.0	35.0	29.0	37.0
88-89	32.99093072195339	34.5	32.0	35.0	28.0	36.0
90-91	32.84818509462656	34.0	32.0	35.0	28.0	36.0
92-93	32.760145946453974	34.0	32.0	35.0	28.0	36.0
94-95	32.69138858067264	34.0	32.0	35.0	28.0	35.0
96-97	32.60003757946693	34.0	32.0	35.0	27.5	35.0
98-99	32.711267533162896	34.0	32.0	35.0	29.0	35.0
100-101	31.77906607999717	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	12.0
20	73.0
21	447.0
22	2143.0
23	7656.0
24	20603.0
25	45195.0
26	82403.0
27	134343.0
28	196861.0
29	270184.0
30	352519.0
31	448034.0
32	569107.0
33	733904.0
34	988829.0
35	1366735.0
36	2077404.0
37	3327682.0
38	3753416.0
39	1629774.0
40	70822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.71076063678234	21.938523139513528	14.863223977240661	24.48749224646348
2	13.920077263687212	22.664891894030127	41.25298981311522	22.16204102916744
3	16.746855219074686	27.95591432271393	31.642545624194128	23.654684834017253
4	11.941098984811196	19.443636754682288	39.90562189184998	28.709642368656528
5	12.417450841816535	38.63115569225732	36.49294287457379	12.45845059135235
6	28.541920906681597	39.842532849090134	19.173565212458872	12.441981031769394
7	24.936045179833226	33.182231758423406	24.863860244591617	17.01786281715175
8	23.590199791058012	37.57197891025626	21.922974084016026	16.914847214669702
9	25.325449506090468	16.275749935611362	21.671595613599003	36.72720494469916
10-11	23.485218787961077	27.124381932818505	30.175392724049605	19.215006555170817
12-13	24.363230414549637	24.814899378641083	29.936105199187445	20.88576500762184
14-15	21.647634519077354	26.128639077625053	27.247172824082277	24.976553579215317
16-17	21.302163703656042	29.10163892987847	27.896894028519004	21.699303337946482
18-19	21.36526989086491	28.514725608616466	29.29758945480471	20.822415045713914
20-21	22.174012860685938	27.78040088288929	28.980210750444694	21.06537550598008
22-23	21.87734106306494	27.72994973862205	28.855137389124234	21.537571809188776
24-25	21.671884675852667	27.842373034535616	28.918777786540566	21.56696450307115
26-27	21.663156496448	27.96917822800582	28.801839904367004	21.56582537117918
28-29	21.65350843756026	28.0359035416975	28.79641318359137	21.514174837150875
30-31	21.868157266186216	27.891091383048654	28.726078359188868	21.51467299157626
32-33	21.667465594571482	28.084599994085412	28.660293875006243	21.58764053633686
34-35	21.78089031153922	28.06425877096988	28.68588624689867	21.468964670592232
36-37	21.80194232787111	28.01161304823917	28.657786279623902	21.528658344265818
38-39	21.649250016237538	28.08977164657392	28.700226868397728	21.56075146879082
40-41	21.75759887839928	27.87775164815282	28.676976726338268	21.68767274710964
42-43	21.779499681420383	28.029699879090252	28.48333975895493	21.70746068053443
44-45	21.689600758096354	28.055098287224727	28.382757526808007	21.872543427870912
46-47	21.76278229361884	27.992252768890523	28.208933794728473	22.036031142762162
48-49	21.71892941681744	28.21885757142158	28.196618291208438	21.865594720552547
50-51	21.69437935362602	28.289837997015095	28.08561830614768	21.93016434321121
52-53	21.769118115129796	28.1589773055888	27.829302355791295	22.242602223490103
54-55	21.69505547013014	27.868779991879787	28.078304721817894	22.357859816172184
56-57	21.790457601657714	27.568824913723834	28.313663214177954	22.327054270440502
58-59	21.594891133728915	27.40277576976901	28.41842745105035	22.583905645451726
60-61	21.663817182739866	27.19212190346138	28.720785821965904	22.42327509183285
62-63	21.391472723017298	27.728500772085724	28.71015458554995	22.169871919347028
64-65	20.987631287219376	27.9269885341363	28.534349627455757	22.551030551188568
66-67	21.11668340252097	28.56096291951188	27.9558711918111	22.36648248615606
68-69	21.210171395719794	28.947457382369475	27.4778413372779	22.36452988463283
70-71	21.236075692360597	28.47716805559035	27.428922836017726	22.857833416031323
72-73	21.120866936960024	28.09934560729112	27.383976400218213	23.395811055530643
74-75	21.53003171402022	27.75409865653688	27.466110143826345	23.249759485616554
76-77	21.97208467860271	27.579858484391977	27.352966143378637	23.095090693626673
78-79	22.206836324693	27.46492177351237	26.652124709219066	23.676117192575568
80-81	22.11340995424583	27.906486177466867	26.568282530001973	23.41182133828533
82-83	21.992176454507163	28.053621244813538	26.666021353226192	23.288180947453103
84-85	22.022431270100597	28.0711616647847	26.618503752031565	23.28790331308314
86-87	22.289093361919615	28.42390733591231	26.590909154996556	22.696090147171518
88-89	22.52213313612535	28.07618494346383	26.905849921156012	22.495831999254815
90-91	22.200798372372894	28.10393148118102	27.245882949518847	22.44938719692724
92-93	21.9371247310018	27.644800784293942	27.068118312470048	23.349956172234208
94-95	21.725892943626	27.605671807990028	27.815508574601395	22.852926673782573
96-97	21.23912512315907	27.9738469104034	28.131743324600304	22.655284641837227
98-99	21.46294027716783	28.063046978149885	28.4961576797112	21.977855064971084
100-101	24.398127660236533	36.06116045566826	29.936883941509585	9.603827942585628
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4753.0
1	4248.0
2	5239.5
3	7131.5
4	9400.0
5	14148.0
6	17891.0
7	20402.0
8	21828.0
9	21327.5
10	19873.0
11	18441.0
12	20248.0
13	26310.5
14	39931.5
15	58499.5
16	72498.0
17	79311.5
18	81078.0
19	79112.0
20	75601.0
21	73779.0
22	75955.5
23	85728.5
24	104228.5
25	130512.0
26	163743.0
27	198779.0
28	234359.0
29	270842.0
30	303510.5
31	337095.0
32	372514.0
33	406932.0
34	440766.0
35	472564.0
36	509038.5
37	543823.0
38	570691.5
39	593280.0
40	614480.5
41	634034.5
42	647807.0
43	659142.0
44	668617.0
45	673998.5
46	674864.5
47	672853.0
48	667475.0
49	654636.5
50	637529.0
51	614567.0
52	581546.5
53	545535.0
54	506872.0
55	464882.5
56	422355.0
57	377803.5
58	330842.5
59	283264.5
60	236730.0
61	191378.0
62	151206.5
63	117086.0
64	86579.0
65	63851.0
66	48144.5
67	36468.0
68	27907.5
69	20313.5
70	14456.5
71	10596.0
72	7645.5
73	5318.5
74	3660.0
75	2582.5
76	1826.5
77	1249.0
78	858.0
79	619.5
80	465.0
81	344.0
82	256.5
83	179.5
84	128.5
85	97.0
86	72.0
87	52.5
88	34.0
89	18.5
90	10.0
91	7.0
92	7.5
93	6.0
94	3.5
95	3.0
96	3.0
97	2.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013620972615812008
3	0.0
4	3.358595987460495E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0034363412649480066
18-19	0.0
20-21	0.0020463325770610596
22-23	0.0
24-25	3.5465848114892175E-4
26-27	0.003420415961795914
28-29	0.0
30-31	1.7461988525103696E-4
32-33	8.627971797035842E-4
34-35	8.688136819649491E-4
36-37	0.010342165877119057
38-39	0.011556997342906331
40-41	0.02483000712901631
42-43	0.0019906809759430866
44-45	0.001411674908468297
46-47	0.0018786734952022024
48-49	0.001692594554343663
50-51	5.3506145699174336E-5
52-53	9.095867104126218E-5
54-55	0.005597855903090891
56-57	0.003322103407176967
58-59	0.003452659151762208
60-61	0.0014037339322598111
62-63	3.436826489408904E-4
64-65	6.913943088450733E-4
66-67	6.115763602117277E-4
68-69	2.135071148399259E-5
70-71	0.0
72-73	1.7893418764061396E-4
74-75	3.1067423409924887E-4
76-77	0.001700717732851858
78-79	0.0018944632129602546
80-81	0.01067126329728013
82-83	0.009494983418583527
84-85	0.012396297570345516
86-87	0.006243560321392706
88-89	0.08425814405653133
90-91	3.751892517117176E-4
92-93	5.528704480793834E-5
94-95	3.0880639283165545E-4
96-97	0.016698569180007485
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	355.0
20-21	1417.0
22-23	3037.0
24-25	6148.0
26-27	8569.0
28-29	15218.0
30-31	37671.0
32-33	49187.0
34-35	67422.0
36-37	105759.0
38-39	81930.0
40-41	121867.0
42-43	134483.0
44-45	155579.0
46-47	156094.0
48-49	145797.0
50-51	139575.0
52-53	24024.0
54-55	23273.0
56-57	23465.0
58-59	25212.0
60-61	164407.0
62-63	156681.0
64-65	162391.0
66-67	173288.0
68-69	179146.0
70-71	180713.0
72-73	173613.0
74-75	172267.0
76-77	173113.0
78-79	189017.0
80-81	194880.0
82-83	186065.0
84-85	185470.0
86-87	197862.0
88-89	212630.0
90-91	233442.0
92-93	250933.0
94-95	318134.0
96-97	420264.0
98-99	1533068.0
100-101	9294681.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.90205717790978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.03987858798982	72.10647170049778
2	7.99522806725063	13.096512526287807
3	2.077738658817257	5.105132113056137
4	0.8256190424561067	2.704795920896447
5	0.40425117600454885	1.6554501465680918
6	0.21236291524024076	1.0435775775884284
7	0.12175168500655999	0.6980199426839896
8	0.08126707570144397	0.532475254462495
9	0.057275665521995166	0.42218953492367556
>10	0.179984160759753	2.255795214906319
>50	0.003481043986701648	0.19100856212473424
>100	0.0011057203190619179	0.14169125647967923
>500	2.8102682715313685E-5	0.017757227711125624
>1k	2.809826317884116E-5	0.029123021813320764
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.219622199000917E-6	0.0
2	0.0	3.7317733194005504E-5	0.0	6.219622199000917E-6	0.0
3	0.0	4.353735539300642E-5	0.0	6.219622199000917E-6	0.0
4	0.0	7.463546638801101E-5	0.0	1.2439244398001834E-5	0.0
5	0.0	7.463546638801101E-5	0.0	1.2439244398001834E-5	6.219622199000917E-6
6	6.219622199000917E-6	7.463546638801101E-5	0.0	1.8658866597002752E-5	1.8658866597002752E-5
7	6.219622199000917E-6	8.085508858701192E-5	0.0	1.8658866597002752E-5	1.8658866597002752E-5
8	6.219622199000917E-6	8.707471078601284E-5	0.0	2.4878488796003667E-5	2.4878488796003667E-5
9	6.219622199000917E-6	1.7414942157202568E-4	0.0	3.7317733194005504E-5	3.7317733194005504E-5
10-11	1.2439244398001834E-5	1.803690437710266E-4	0.0	4.042754429350596E-5	3.7317733194005504E-5
12-13	1.8658866597002752E-5	1.8347885487052706E-4	0.0	5.286678869150779E-5	3.7317733194005504E-5
14-15	1.8658866597002752E-5	2.176867769650321E-4	0.0	5.5976599791008256E-5	5.9086410890508714E-5
16-17	1.8658866597002752E-5	2.176867769650321E-4	0.0	6.219622199000918E-5	9.329433298501375E-5
18-19	1.8658866597002752E-5	2.705535656565399E-4	0.0	8.085508858701192E-5	9.951395518401467E-5
20-21	1.8658866597002752E-5	3.358595987460495E-4	0.0	1.0573357738301558E-4	1.0884338848251605E-4
22-23	2.4878488796003667E-5	3.638478986415537E-4	0.0	1.7414942157202568E-4	1.2439244398001836E-4
24-25	4.353735539300642E-5	4.13604876233561E-4	0.0	3.7939695413905596E-4	1.3683168837802019E-4
26-27	5.908641089050872E-5	4.291539317310633E-4	0.0	7.836723970741156E-4	1.3683168837802019E-4
28-29	6.219622199000918E-5	4.353735539300642E-4	0.0	0.003486098242540014	1.3683168837802019E-4
30-31	6.219622199000918E-5	5.100090203180752E-4	0.0	0.012653821363867366	1.3683168837802019E-4
32-33	6.841584418901009E-5	5.131188314175756E-4	0.0	0.03343357913072943	1.586003660745234E-4
34-35	7.774527748751147E-5	6.219622199000917E-4	0.0	0.06011575836444336	1.865886659700275E-4
36-37	8.707471078601284E-5	7.308056083826078E-4	0.0	0.09671823500556376	1.8969847706952795E-4
38-39	8.707471078601284E-5	7.4635466388011E-4	0.0	0.16577159046997145	2.1457696586553167E-4
40-41	9.329433298501375E-5	8.209901302681211E-4	0.0	0.4841322821591319	2.3012602136303394E-4
42-43	9.329433298501375E-5	8.551980523626261E-4	0.0	0.5438468748917398	2.3012602136303394E-4
44-45	9.329433298501375E-5	9.858101185416454E-4	0.0	0.6351509287730732	2.3012602136303394E-4
46-47	9.329433298501375E-5	0.0010759946404271586	0.0	0.7508141329967937	2.5189469905953716E-4
48-49	9.329433298501375E-5	0.0010822142626261597	0.0	0.8462106982850698	2.7988299895504126E-4
50-51	9.329433298501375E-5	0.001172398784511673	0.0	0.9556294018209934	2.923222433530431E-4
52-53	9.329433298501375E-5	0.0011786184067106738	0.0	1.193775626009639	3.2653016544754814E-4
54-55	9.329433298501375E-5	0.0012283753843026811	0.0	1.9654317129952847	3.638478986415537E-4
56-57	9.329433298501375E-5	0.0012781323618946887	0.0	2.8064085991998953	3.7628714303955546E-4
58-59	9.329433298501375E-5	0.0012905716062926904	0.0	3.6795533714177386	3.918361985370578E-4
60-61	9.329433298501375E-5	0.0013372187727851972	0.0	4.357632132608316	4.042754429350596E-4
62-63	9.329433298501375E-5	0.0013776463170787033	0.0	4.374453100845514	4.291539317310633E-4
64-65	9.329433298501375E-5	0.001505148572158222	0.0	4.3851197529168005	4.322637428305638E-4
66-67	9.329433298501375E-5	0.0015206976276557244	0.0	4.393444717230164	4.353735539300642E-4
68-69	9.329433298501375E-5	0.001579784038546233	0.0	4.400774541991686	4.353735539300642E-4
70-71	9.329433298501375E-5	0.0016233213939392394	0.0	4.408272296552582	4.5403242052706694E-4
72-73	9.329433298501375E-5	0.0016388704494367417	0.0	4.414495028562682	4.8513053152207154E-4
74-75	9.329433298501375E-5	0.0016917372381282493	0.0	4.4199496372312055	4.8513053152207154E-4
76-77	9.329433298501375E-5	0.0017819217600137628	0.0	4.424797832735328	4.8513053152207154E-4
78-79	9.329433298501375E-5	0.00183167873760577	0.0	4.429254192040911	4.8513053152207154E-4
80-81	9.329433298501375E-5	0.0018441179820037719	0.0	4.431919300153183	4.944599648205729E-4
82-83	9.329433298501375E-5	0.0018596670375012742	0.0	4.4336918924798985	5.100090203180752E-4
84-85	9.329433298501375E-5	0.001884545526297278	0.0	4.435184601807658	5.131188314175756E-4
86-87	9.329433298501375E-5	0.0019218632594912835	0.0	4.435377410095827	5.22448264716077E-4
88-89	9.329433298501375E-5	0.0019996085369787947	0.0	4.435411618017922	5.348875091140789E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17215	0.0	47.272808	1
TATCTCG	20065	0.0	40.53436	94-95
GTATCAA	29890	0.0	33.87687	1
CCGTCTT	8360	0.0	31.02168	92-93
TGCCGTC	8205	0.0	30.835506	90-91
TATGCCG	8060	0.0	30.62491	88-89
GCCGTCT	8510	0.0	29.047316	90-91
ATGCCGT	8430	0.0	28.329395	88-89
CTATCTC	27620	0.0	28.085918	94-95
TCAACGC	36350	0.0	26.980032	4
ATCAACG	36525	0.0	26.88565	3
CAACGCA	37155	0.0	26.429697	5
CGTATGC	9545	0.0	26.26399	86-87
AACGCAG	37660	0.0	26.109125	6
ATCTCGT	15750	0.0	25.846981	94-95
TATCAAC	39300	0.0	25.235811	2
CGCTATC	29265	0.0	25.110336	92-93
GCTATCT	34390	0.0	25.015747	94-95
CTCGTAT	9895	0.0	24.71324	84-85
TCGTATG	9695	0.0	24.63579	84-85
>>END_MODULE