FastQCFastQC Report
Fri 27 May 2016
SRR2138785_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138785_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18750789
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205500.043.8850981
GTATCAA357550.033.957271
TCAACGC435550.026.9171854
ATCAACG436950.026.8118323
CAACGCA442150.026.515325
AACGCAG452650.026.0293836
TATCAAC471250.025.3478832
ACGCAGA523250.022.4215957
CGCAGAG528900.022.1583928
GTACATG489600.020.5433181
TACATGG494500.019.9766942
ACATGGG495300.019.379443
AGAGTAC567600.018.37549810-11
GCAGAGT638700.018.2249039
CATGGGG350950.017.6070674
GAGTACT371050.017.3066512-13
TATACCG15300.016.9188045
CAGAGTA620650.016.50885210-11
AGTACTT420300.015.77539412-13
GTACTTT428450.014.97926714-15
Produced by FastQC (version 0.11.5)