FastQCFastQC Report
Fri 27 May 2016
SRR2138784_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138784_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41227851
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA482650.044.4536861
GTATCAA824350.033.2489281
ATCAACG997350.026.648673
TCAACGC999600.026.5764854
CAACGCA1015850.026.1390955
AACGCAG1036750.025.7002496
TATCAAC1062500.025.4208562
ACGCAGA1196700.022.1645957
CGCAGAG1206450.021.9648238
GTACATG1077850.019.1267871
TACATGG1048100.019.090592
ACATGGG1054750.018.3300723
GCAGAGT1447600.018.128319
AGAGTAC1300600.017.80846210-11
GAGTACT869150.017.27462212-13
CATGGGG739500.016.6792354
CAGAGTA1428050.016.49673510-11
AGTACTT958700.015.55710912-13
GTACTTT1020950.014.70811714-15
TATACTG260600.014.2739865
Produced by FastQC (version 0.11.5)