##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138784_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41227851
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.433532080049478	31.0	31.0	34.0	30.0	34.0
2	31.559093342022607	31.0	31.0	34.0	30.0	34.0
3	31.595098274707553	31.0	31.0	34.0	30.0	34.0
4	35.41262077909421	37.0	35.0	37.0	33.0	37.0
5	35.23671583076207	37.0	35.0	37.0	32.0	37.0
6	35.253198960091325	37.0	35.0	37.0	32.0	37.0
7	35.15782661094802	37.0	35.0	37.0	32.0	37.0
8	35.15212352445923	37.0	35.0	37.0	32.0	37.0
9	36.68016804950614	39.0	35.0	39.0	32.0	39.0
10-11	36.5610205101401	39.0	35.0	39.0	32.0	39.0
12-13	36.5045160612422	38.0	35.0	39.0	32.0	39.0
14-15	37.56650663164568	39.0	36.0	41.0	32.0	41.0
16-17	37.54491424983563	39.0	36.0	41.0	32.0	41.0
18-19	37.55270056399496	39.0	36.0	41.0	32.0	41.0
20-21	37.53336835405935	39.0	36.0	41.0	32.0	41.0
22-23	37.441540848846444	39.0	36.0	41.0	32.0	41.0
24-25	37.33195969183521	39.0	36.0	41.0	31.0	41.0
26-27	37.118712087518574	39.0	36.0	41.0	31.0	41.0
28-29	36.93969110833762	39.0	36.0	40.0	30.5	41.0
30-31	36.778639612231544	39.0	35.5	40.0	30.0	41.0
32-33	36.59973077016352	38.0	35.0	40.0	30.0	41.0
34-35	36.45948316895818	38.0	35.0	40.0	30.0	41.0
36-37	36.30668899017415	38.0	35.0	40.0	30.0	41.0
38-39	36.14524799540466	38.0	35.0	40.0	30.0	41.0
40-41	35.94514509629637	38.0	34.0	40.0	29.0	41.0
42-43	35.744429518570485	38.0	34.0	40.0	28.0	41.0
44-45	35.94321411281197	38.0	34.0	40.0	29.0	41.0
46-47	35.92509359275833	38.0	34.0	40.0	29.0	41.0
48-49	36.27086566173855	38.0	35.0	40.0	30.0	41.0
50-51	36.33794204793979	38.0	35.0	40.0	29.5	41.0
52-53	36.261823172147075	38.0	34.5	40.0	29.0	41.0
54-55	36.13731049758084	38.0	34.0	40.0	29.0	41.0
56-57	36.01818259022049	38.0	34.0	40.0	28.5	41.0
58-59	35.84702735976788	38.0	34.0	40.0	28.0	41.0
60-61	35.64828557878903	38.0	34.0	40.0	28.0	41.0
62-63	35.43051701454207	37.0	33.5	40.0	28.0	41.0
64-65	35.206336494278546	37.0	33.0	40.0	28.0	41.0
66-67	34.9365129222158	36.5	33.0	39.0	28.0	41.0
68-69	34.66941129119606	36.0	32.5	39.0	27.0	41.0
70-71	34.421474561150475	36.0	32.0	39.0	26.5	40.0
72-73	34.10885926964223	35.0	32.0	38.5	26.0	40.0
74-75	33.79340991367976	35.0	32.0	38.0	26.0	40.0
76-77	31.929592873998793	33.5	29.5	35.5	25.0	38.5
78-79	33.057699854276365	34.5	31.0	36.0	26.0	39.0
80-81	33.14472567504049	35.0	31.0	36.0	26.0	39.0
82-83	32.93036404655451	34.0	31.0	36.0	26.0	37.5
84-85	32.698883979528375	34.0	31.0	35.5	26.0	37.0
86-87	32.51119761134653	34.0	31.0	35.0	26.0	37.0
88-89	32.34554349492648	34.0	31.0	35.0	26.0	36.0
90-91	32.17207124070349	34.0	31.0	35.0	26.0	36.0
92-93	32.15125656533482	34.0	31.0	35.0	26.0	36.0
94-95	32.160058484960764	34.0	31.0	35.0	26.0	35.0
96-97	32.26645241715178	34.0	31.0	35.0	26.5	35.0
98-99	32.41955551268395	34.0	31.0	35.0	27.0	35.0
100	30.933269798713575	32.0	29.0	34.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	45.0
20	612.0
21	4009.0
22	18735.0
23	62154.0
24	154014.0
25	308753.0
26	522094.0
27	777880.0
28	1052622.0
29	1329063.0
30	1604121.0
31	1891340.0
32	2231243.0
33	2652542.0
34	3230116.0
35	4010781.0
36	5221319.0
37	6751772.0
38	6553403.0
39	2772502.0
40	78726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.50151318825713	21.966182520646054	14.611620188498303	23.920684102598507
2	14.40459812414768	22.86250268501437	41.13333205741515	21.5995671334228
3	17.414459824895054	28.155035834737745	31.404263727040245	23.026240613326955
4	12.227607982768737	19.619070128103452	40.00689242813068	28.14642946099713
5	12.215075192738036	38.898188023431054	36.609572495059226	12.277164288771685
6	27.908644222682135	40.37423269357527	19.439439262197315	12.27768382154528
7	24.522110065838746	33.421540695875706	25.152982143066343	16.9033670952192
8	23.23888043546097	38.00211900445648	22.108161786070298	16.650838774012257
9	25.09340336215477	16.501055683755496	21.98282987282434	36.4227110812654
10-11	23.17318020157696	27.44553219132948	30.33131565560539	19.049971951488175
12-13	24.161504856869836	25.07268762515224	30.15691989102108	20.608887626956847
14-15	21.38718777075727	26.387925081293655	27.524999945121635	24.69988720282744
16-17	20.955192227935218	29.35923803292162	28.186797765090166	21.498771974053
18-19	20.978873351489998	28.7291903945674	29.689290507510506	20.602645746432096
20-21	21.770018603961226	27.982879285780605	29.409023282029366	20.838078828228802
22-23	21.51678059876385	27.947307393211823	29.285730272464743	21.250181735559583
24-25	21.306303808205694	28.095819486572104	29.319829694696736	21.278047010525462
26-27	21.331900588337703	28.18945855113033	29.196399741876643	21.282241118655325
28-29	21.306298840409756	28.22945453376113	29.198901036319718	21.265345589509398
30-31	21.571374749464844	28.081651483916755	29.09431077497454	21.25266299164386
32-33	21.411784474524133	28.31301100156508	29.01465748285591	21.260547041054874
34-35	21.47379242071627	28.343567262834174	29.074032980788328	21.10860733566123
36-37	21.497960378897314	28.260043488032483	28.97002258366288	21.271973549407324
38-39	21.407793552191578	28.292183373829182	29.04280833489285	21.257214739086386
40-41	21.65274897435565	28.164245297839475	28.907085660366317	21.275920067438562
42-43	21.45836115285298	28.34126907160246	28.71386055577811	21.48650921976645
44-45	21.537447952607906	28.466237920789155	28.556161792760015	21.440152333842924
46-47	21.623900761771374	28.41055381715843	28.321743505463417	21.64380191560678
48-49	21.567578661035565	28.495633943631237	28.32631923957316	21.610468155760028
50-51	21.546207367383364	28.59188684097068	28.20989827100814	21.652007520637824
52-53	21.628231254417727	28.50664482049564	28.155890518843613	21.709233406243023
54-55	21.62551386173151	28.424350591047187	28.233471051664534	21.716664495556774
56-57	21.616359488240004	28.46562591309261	28.21524644520433	21.702768153463058
58-59	21.60324060905374	28.533918154200126	28.197279759596995	21.66556147714914
60-61	21.697430909759248	28.542004143100634	28.163778254599254	21.596786692540864
62-63	21.64867030979959	28.57398572384965	28.123558558301504	21.653785408049256
64-65	21.583842086004097	28.55474813846422	28.102035088808574	21.759374686723106
66-67	21.623009114767537	28.603463496672383	27.98044749662071	21.793079891939367
68-69	21.554772806263514	28.598826632927697	28.043332193483916	21.80306836732487
70-71	21.61455970933567	28.362375505474702	28.087745167967533	21.935319617222092
72-73	21.605905498461787	28.437106622199988	28.037450152818504	21.91953772651972
74-75	21.50549966939804	28.53414746660956	28.07213687186935	21.88821599212304
76-77	21.587225992071865	28.47989062293699	28.014109154405414	21.918774230585733
78-79	21.57428361583807	28.504959264636614	27.986955880229107	21.933801239296212
80-81	21.596361307017105	28.521618602337984	27.989609161955403	21.892410928689507
82-83	21.627345703671168	28.44220848217282	28.127606999482456	21.802838814673557
84-85	21.682765130584343	28.476743842557738	28.081652694241072	21.758838332616843
86-87	21.488496861632928	28.525020817258067	28.180362759808542	21.806119561300463
88-89	21.557165884749235	28.473301446294318	28.14988666385047	21.819646005105973
90-91	21.57905717282699	28.444698567464894	28.25824459586177	21.71799966384635
92-93	21.54625611578735	28.45942575335258	28.262479202690365	21.731838928169704
94-95	21.30229765157199	28.53618343621577	28.401843852813013	21.75967505939923
96-97	21.30932018716791	28.673680205010815	28.552493044450856	21.464506563370417
98-99	22.320455058372588	30.66551014049986	28.811891767190644	18.20214303393691
100	21.798804804611482	31.34634115458848	22.595538005979176	24.25931603482086
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11991.0
1	10608.0
2	14363.0
3	20462.0
4	27065.0
5	43497.0
6	57671.0
7	66384.0
8	69853.5
9	65684.5
10	58321.0
11	51289.5
12	54402.0
13	71040.5
14	109115.5
15	164568.0
16	209875.0
17	232502.0
18	238927.5
19	236445.5
20	228420.5
21	223938.5
22	230530.5
23	256892.0
24	308513.5
25	382751.5
26	477422.5
27	576160.0
28	672150.0
29	775519.0
30	869339.5
31	961203.5
32	1065565.0
33	1159029.0
34	1245658.5
35	1329219.0
36	1416039.5
37	1504403.5
38	1567206.0
39	1602094.5
40	1636212.0
41	1666426.0
42	1688102.5
43	1711659.0
44	1723249.0
45	1734128.0
46	1738669.5
47	1726416.5
48	1698453.5
49	1646137.0
50	1587804.5
51	1519822.5
52	1431678.0
53	1337382.5
54	1238093.0
55	1127979.5
56	1020718.5
57	915387.5
58	807239.5
59	698306.0
60	580352.0
61	466167.5
62	368267.5
63	284841.5
64	213117.0
65	157478.5
66	117418.0
67	89005.5
68	68398.0
69	50372.5
70	36537.5
71	27173.0
72	19731.5
73	13926.0
74	9978.0
75	7129.0
76	5075.5
77	3589.5
78	2546.5
79	1860.0
80	1354.0
81	964.0
82	718.0
83	515.5
84	363.5
85	265.0
86	207.0
87	167.0
88	124.5
89	86.0
90	57.0
91	46.0
92	44.5
93	31.5
94	13.0
95	7.0
96	7.0
97	5.5
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.13313087795917375
3	2.8621428752131656E-4
4	0.0
5	0.0
6	6.306416504706977E-5
7	0.0
8	0.0
9	0.002464353526454726
10-11	4.208320244487155E-4
12-13	1.5280932299866905E-4
14-15	7.531316633505831E-4
16-17	0.0012261129012036062
18-19	3.638317214254025E-5
20-21	0.0027713839867128816
22-23	3.881876111209782E-5
24-25	8.203323399302859E-4
26-27	0.004915191037799968
28-29	0.001280912028809097
30-31	0.005884072524103031
32-33	0.005034891062716837
34-35	4.042407153992487E-5
36-37	8.74118168661864E-5
38-39	0.0
40-41	0.0
42-43	2.265842715621558E-5
44-45	0.0
46-47	0.0
48-49	2.322413324258709E-4
50-51	0.0
52-53	2.6427245337715285E-6
54-55	3.598155249791133E-5
56-57	0.0
58-59	0.0
60-61	0.0
62-63	3.1379168755545073E-4
64-65	1.4034523974629958E-6
66-67	0.0
68-69	1.4370868662676855E-6
70-71	5.82260242263348E-6
72-73	4.424584027157271E-6
74-75	3.286746195964772E-5
76-77	7.385542703298717E-4
78-79	0.00423450651079514
80-81	3.8863881880910525E-5
82-83	6.313780229432672E-6
84-85	3.2056466054470443E-6
86-87	1.6287341009492002E-6
88-89	0.0
90-91	8.822922686119809E-5
92-93	6.065953812228626E-4
94-95	0.0
96-97	1.855852347793337E-6
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1767.0
20-21	6270.0
22-23	12294.0
24-25	21882.0
26-27	31982.0
28-29	51336.0
30-31	107610.0
32-33	133690.0
34-35	191126.0
36-37	251715.0
38-39	300004.0
40-41	315411.0
42-43	364245.0
44-45	403996.0
46-47	402195.0
48-49	376535.0
50-51	338016.0
52-53	316148.0
54-55	338202.0
56-57	370258.0
58-59	389633.0
60-61	396326.0
62-63	383251.0
64-65	399710.0
66-67	424355.0
68-69	437418.0
70-71	452282.0
72-73	433689.0
74-75	442303.0
76-77	398911.0
78-79	452045.0
80-81	485664.0
82-83	483110.0
84-85	486895.0
86-87	530683.0
88-89	657509.0
90-91	690514.0
92-93	748808.0
94-95	923330.0
96-97	1819688.0
98-99	2964321.0
100-101	2.2492724E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.78579708641954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.86167672223665	70.10113910632964
2	7.61110466588896	12.14516104952235
3	2.149923879286957	5.146001711521112
4	0.926138384848808	2.9557075698996527
5	0.49376473461672893	1.9697706462280065
6	0.27709269305196016	1.3264836829183935
7	0.1798348670449506	1.0043787747778168
8	0.12218843269537348	0.7799121197872559
9	0.08440486439188272	0.6060878445129761
>10	0.28714320970916096	3.444320366043232
>50	0.005241972113123462	0.2810604972028255
>100	0.0014302149297224056	0.17932475395519729
>500	2.8340470777409308E-5	0.013033022474298045
>1k	2.701871496961194E-5	0.04761885482722113
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.3361985809059024E-5	0.0	4.851089619005366E-6	0.0
3	0.0	5.3361985809059024E-5	0.0	4.851089619005366E-6	0.0
4	0.0	1.576604126176744E-4	0.0	4.851089619005366E-6	2.425544809502683E-6
5	0.0	1.600859574271771E-4	0.0	7.276634428508049E-6	2.425544809502683E-6
6	0.0	1.6251150223667977E-4	0.0	7.276634428508049E-6	9.702179238010731E-6
7	0.0	1.697881366651878E-4	0.0	7.276634428508049E-6	9.702179238010731E-6
8	0.0	1.8919249514120928E-4	0.0	7.276634428508049E-6	1.2127724047513415E-5
9	0.0	3.104697356163434E-4	0.0	7.276634428508049E-6	2.1829903285524148E-5
10-11	0.0	3.104697356163434E-4	0.0	7.276634428508049E-6	2.1829903285524148E-5
12-13	1.2127724047513414E-6	3.104697356163434E-4	0.0	7.276634428508049E-6	2.1829903285524148E-5
14-15	3.6383172142540243E-6	4.329597484962289E-4	0.0	7.276634428508049E-6	3.274485492828622E-5
16-17	4.851089619005366E-6	4.463002449484937E-4	0.0	7.276634428508049E-6	3.638317214254025E-5
18-19	4.851089619005366E-6	4.875345067100393E-4	0.0	9.702179238010731E-6	4.244703416629696E-5
20-21	4.851089619005366E-6	5.396837201143471E-4	0.0	1.5766041261767438E-5	4.7298123785302314E-5
22-23	4.851089619005366E-6	6.233650160421896E-4	0.0	3.1532082523534876E-5	5.0936440999556346E-5
24-25	4.851089619005366E-6	6.852164086845079E-4	0.0	7.03407994755778E-5	5.578753061856171E-5
26-27	4.851089619005366E-6	7.167484912080429E-4	0.0	1.7827754349844722E-4	5.8213075428064395E-5
28-29	4.851089619005366E-6	7.203868084222969E-4	0.0	9.884095098723433E-4	5.8213075428064395E-5
30-31	7.276634428508049E-6	7.955786975168801E-4	0.0	0.0038857227848232982	5.8213075428064395E-5
32-33	8.48940683325939E-6	8.004297871358854E-4	0.0	0.009897435595175698	6.0638620237567074E-5
34-35	9.702179238010731E-6	9.132176207777602E-4	0.0	0.018561481654719283	6.791525466607512E-5
36-37	9.702179238010731E-6	0.0010308565440386402	0.0	0.03166791303286703	6.791525466607512E-5
38-39	9.702179238010731E-6	0.0010611758541574237	0.0	0.06560977432464282	7.03407994755778E-5
40-41	9.702179238010731E-6	0.0011703253705850447	0.0	0.099432783920753	7.03407994755778E-5
42-43	9.702179238010731E-6	0.0012091340875370876	0.0	0.1241224045366808	7.03407994755778E-5
44-45	9.702179238010731E-6	0.0013546667761072484	0.0	0.15452175763417791	7.03407994755778E-5
46-47	9.702179238010731E-6	0.001480795106201388	0.0	0.19048167220745996	7.397911668983183E-5
48-49	9.702179238010731E-6	0.0014856461958203933	0.0	0.2213091339638343	7.519188909458317E-5
50-51	9.702179238010731E-6	0.0016323916567953056	0.0	0.24771967862210426	7.519188909458317E-5
52-53	9.702179238010731E-6	0.001648157698057073	0.0	0.30534698497867374	8.610684073734524E-5
54-55	9.702179238010731E-6	0.0016493704704618245	0.0	0.3753651869945877	9.823456478485866E-5
56-57	9.702179238010731E-6	0.0016712003737473487	0.0	0.44975785907443977	1.0066010959436134E-4
58-59	9.702179238010731E-6	0.0016845408701996133	0.0	0.5085615546636181	1.0551119921336672E-4
60-61	9.702179238010731E-6	0.0017160729527231481	0.0	0.5367197043571347	1.0914951642762074E-4
62-63	9.702179238010731E-6	0.0017633710765084507	0.0	0.565655241162097	1.0914951642762074E-4
64-65	9.702179238010731E-6	0.0019452869372211519	0.0	0.5790551634621945	1.0914951642762074E-4
66-67	9.702179238010731E-6	0.001957414661268665	0.0	0.5900974562074555	1.1400060604662611E-4
68-69	9.702179238010731E-6	0.0020156277366967295	0.0	0.6041413606544761	1.2127724047513415E-4
70-71	9.702179238010731E-6	0.0020423087296012592	0.0	0.61455907561129	1.261283300941395E-4
72-73	9.702179238010731E-6	0.0020556492260535237	0.0	0.6220503707554391	1.3583050933215025E-4
74-75	9.702179238010731E-6	0.002113862301481588	0.0	0.627927465828864	1.3583050933215025E-4
76-77	9.702179238010731E-6	0.0022096713214569443	0.0	0.631907784861258	1.3583050933215025E-4
78-79	9.702179238010731E-6	0.0022509055832184897	0.0	0.6355000167241315	1.3583050933215025E-4
80-81	1.0914951642762074E-5	0.0022569694452422464	0.0	0.63643385147579	1.4189437135590696E-4
82-83	1.2127724047513415E-5	0.002266671624480257	0.0	0.6370365993609515	1.5038377818916634E-4
84-85	1.2127724047513415E-5	0.0022739482589087655	0.0	0.6373410052345441	1.5038377818916634E-4
86-87	1.2127724047513415E-5	0.0023066931138370516	0.0	0.6375035167367806	1.5038377818916634E-4
88	1.2127724047513415E-5	0.002389161637360143	0.0	0.6375180700056376	1.5038377818916634E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	48265	0.0	44.453686	1
GTATCAA	82435	0.0	33.248928	1
ATCAACG	99735	0.0	26.64867	3
TCAACGC	99960	0.0	26.576485	4
CAACGCA	101585	0.0	26.139095	5
AACGCAG	103675	0.0	25.700249	6
TATCAAC	106250	0.0	25.420856	2
ACGCAGA	119670	0.0	22.164595	7
CGCAGAG	120645	0.0	21.964823	8
GTACATG	107785	0.0	19.126787	1
TACATGG	104810	0.0	19.09059	2
ACATGGG	105475	0.0	18.330072	3
GCAGAGT	144760	0.0	18.12831	9
AGAGTAC	130060	0.0	17.808462	10-11
GAGTACT	86915	0.0	17.274622	12-13
CATGGGG	73950	0.0	16.679235	4
CAGAGTA	142805	0.0	16.496735	10-11
AGTACTT	95870	0.0	15.557109	12-13
GTACTTT	102095	0.0	14.708117	14-15
TATACTG	26060	0.0	14.273986	5
>>END_MODULE