FastQCFastQC Report
Fri 27 May 2016
SRR2138783_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138783_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41274488
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA464150.047.600171
GTATCAA826050.034.521771
TCAACGC1018950.027.2917774
ATCAACG1019850.027.279663
CAACGCA1037250.026.7863485
AACGCAG1064250.026.1572996
TATCAAC1086900.025.84812
ACGCAGA1235250.022.516197
CGCAGAG1248750.022.2396538
GTACATG1085900.020.3192081
TACATGG1068250.020.096692
ACATGGG1077900.019.2917043
GCAGAGT1486200.018.383289
AGAGTAC1324800.018.14391310-11
GAGTACT897650.017.37890412-13
CAGAGTA1447350.016.85480310-11
CATGGGG748650.016.8546734
AGTACTT994900.015.72586812-13
GTGGTAT228000.014.9666551
GTACTTT1053000.014.80676714-15
Produced by FastQC (version 0.11.5)