##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138783_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41274488
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.442559772031576	31.0	31.0	34.0	30.0	34.0
2	31.56930358530432	31.0	31.0	34.0	30.0	34.0
3	31.60530708460878	31.0	31.0	34.0	30.0	34.0
4	35.422124775963304	37.0	35.0	37.0	33.0	37.0
5	35.24750446328977	37.0	35.0	37.0	33.0	37.0
6	35.26257859334318	37.0	35.0	37.0	32.0	37.0
7	35.16543432349785	37.0	35.0	37.0	32.0	37.0
8	35.16041546051401	37.0	35.0	37.0	32.0	37.0
9	36.686907491135926	39.0	35.0	39.0	32.0	39.0
10-11	36.57250481217356	39.0	35.0	39.0	32.0	39.0
12-13	36.51713987342496	39.0	35.0	39.0	32.0	39.0
14-15	37.58131381302658	39.0	36.0	41.0	32.0	41.0
16-17	37.560485462593746	39.0	36.0	41.0	32.0	41.0
18-19	37.57175219229855	39.0	36.0	41.0	32.0	41.0
20-21	37.55270177146461	39.0	36.0	41.0	32.0	41.0
22-23	37.46222701766041	39.0	36.0	41.0	32.0	41.0
24-25	37.35383782750306	39.0	36.0	41.0	31.0	41.0
26-27	37.144839391932194	39.0	36.0	41.0	31.0	41.0
28-29	36.966051385141654	39.0	36.0	40.0	30.5	41.0
30-31	36.804822067058495	39.0	35.5	40.0	30.0	41.0
32-33	36.626943786302505	38.0	35.0	40.0	30.0	41.0
34-35	36.48815847869214	38.0	35.0	40.0	30.0	41.0
36-37	36.334938423515965	38.0	35.0	40.0	30.0	41.0
38-39	36.177445639535875	38.0	35.0	40.0	30.0	41.0
40-41	35.98200122748489	38.0	34.0	40.0	29.0	41.0
42-43	35.784774710639226	38.0	34.0	40.0	28.5	41.0
44-45	35.98512882946689	38.0	34.0	40.0	29.0	41.0
46-47	35.9707928901539	38.0	34.0	40.0	29.0	41.0
48-49	36.31134334491141	38.0	35.0	40.0	30.0	41.0
50-51	36.3812745561531	38.0	35.0	40.0	30.0	41.0
52-53	36.31054246757448	38.0	35.0	40.0	29.0	41.0
54-55	36.188981794146116	38.0	34.0	40.0	29.0	41.0
56-57	36.07678686250183	38.0	34.0	40.0	28.5	41.0
58-59	35.910528666555024	38.0	34.0	40.0	28.0	41.0
60-61	35.71498316458066	38.0	34.0	40.0	28.0	41.0
62-63	35.496094444538414	37.5	34.0	40.0	28.0	41.0
64-65	35.27023665133677	37.0	33.0	40.0	28.0	41.0
66-67	34.999862317854124	36.5	33.0	39.5	28.0	41.0
68-69	34.729573762690954	36.0	33.0	39.0	27.5	41.0
70-71	34.47909659663897	36.0	32.0	39.0	27.0	40.0
72-73	34.162146825395624	35.0	32.0	38.5	26.0	40.0
74-75	33.84651881186238	35.0	32.0	38.0	26.0	40.0
76-77	31.98186489636888	33.5	29.5	35.5	25.0	38.5
78-79	33.10738151408967	34.5	31.0	36.0	26.0	39.0
80-81	33.190305876514536	35.0	32.0	36.0	26.0	39.0
82-83	32.97789823101243	34.0	31.0	36.0	26.0	37.5
84-85	32.74973329599851	34.0	31.0	35.5	26.0	37.0
86-87	32.56232609429036	34.0	31.0	35.0	26.0	37.0
88-89	32.39686666496672	34.0	31.0	35.0	26.0	36.0
90-91	32.219309630017634	34.0	31.0	35.0	26.0	36.0
92-93	32.192952885894655	34.0	31.0	35.0	26.0	36.0
94-95	32.20151634274313	34.0	31.0	35.0	26.0	35.0
96-97	32.30525272427847	34.0	31.0	35.0	27.0	35.0
98-99	32.45311693529845	34.0	31.0	35.0	27.0	35.0
100	30.986829677452107	33.0	29.0	34.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	0.0
15	0.0
16	2.0
17	0.0
18	19.0
19	89.0
20	650.0
21	4055.0
22	18507.0
23	60281.0
24	149530.0
25	300891.0
26	508811.0
27	761004.0
28	1036391.0
29	1311318.0
30	1590297.0
31	1885294.0
32	2221308.0
33	2644599.0
34	3213769.0
35	3990326.0
36	5198390.0
37	6768830.0
38	6627336.0
39	2893645.0
40	89144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.69115109777137	21.778550512329808	14.63734970225069	23.892948687648136
2	14.532640700471255	22.83072196990267	40.9750899432247	21.661547386401374
3	17.479412591525605	27.964970714641158	31.42621237257137	23.129404321261866
4	12.31683843055788	19.53420233825796	39.8347715421691	28.314187689015064
5	12.312176955411294	38.786780347220784	36.490805167589244	12.410237529778685
6	28.07152073321847	40.143367091493495	19.38335724036641	12.401754934921627
7	24.64924397485546	33.41712520860647	25.006125454690693	16.92750536184738
8	23.31129639479226	37.833836674941594	22.08445134642134	16.77041558384481
9	25.18511571187223	16.499735944428664	21.96245780798526	36.35269053571384
10-11	23.289363320207208	27.359772406577378	30.25889825130544	19.091966021909975
12-13	24.222283190814537	25.05518730369661	30.074962375624292	20.647567129864562
14-15	21.4629455348104	26.342386491363033	27.51755115106245	24.67711682276412
16-17	21.026166451395834	29.298702294895186	28.1547126916642	21.520418562044778
18-19	21.0580046597087	28.610465329190127	29.70147998392759	20.630050027173578
20-21	21.89002307339569	27.89626240066294	29.420812336179797	20.792902189761566
22-23	21.587591310084214	27.816915424043014	29.32578246706553	21.269710798807235
24-25	21.38765942880973	27.94994003466657	29.37262046428263	21.289780072241072
26-27	21.40691749109587	28.054941408154683	29.27559272299615	21.262548377753294
28-29	21.404039204803556	28.08745111721162	29.27284917552062	21.2356605024642
30-31	21.651746127406195	27.999516724894807	29.127648672909224	21.221088474789777
32-33	21.46152287061352	28.176814525119898	29.078958665829628	21.282703938436953
34-35	21.548807841968095	28.188246567920494	29.072744814046192	21.190200776065222
36-37	21.60090806356017	28.124507396325548	29.014877060088484	21.2597074800258
38-39	21.44530571366147	28.232297079771534	29.054111495081585	21.26828571148541
40-41	21.713957231535996	28.102730118203866	28.893272869470486	21.290039780789645
42-43	21.57007601034964	28.24315917053873	28.71414265712045	21.47262216199118
44-45	21.61461382953495	28.365274699087113	28.529205740447612	21.490905730930333
46-47	21.729699486383687	28.30772475979201	28.294146227429806	21.668429526394505
48-49	21.656776302207415	28.393542054426973	28.317648855985528	21.632032787380084
50-51	21.637737318439733	28.50937629450047	28.179860339978895	21.6730260470809
52-53	21.769495603053887	28.398803586801407	28.117701794880844	21.71399901526386
54-55	21.75863435594289	28.319035112987983	28.197932829415045	21.724397701654087
56-57	21.717282653514957	28.418382312364905	28.187236245670633	21.67709878844951
58-59	21.73377210196119	28.456350878137084	28.1318745257579	21.67800249414383
60-61	21.790921803226716	28.457446224514165	28.11231336077012	21.639318611488996
62-63	21.752775440750185	28.495154261832184	28.087630450608987	21.664439846808644
64-65	21.71363957305128	28.45488717114153	28.1080374870953	21.72343576871189
66-67	21.804457906426645	28.486226424956136	27.962983064226066	21.746332604391153
68-69	21.726540324668143	28.47032592336396	28.039835848719623	21.763297903248272
70-71	21.76190802117562	28.20010473765227	28.143227114030754	21.894760127141353
72-73	21.78756101634815	28.290052716320385	28.076417593221652	21.845968674109812
74-75	21.630498852225323	28.396387246784982	28.088937345241057	21.884176555748642
76-77	21.708129847762407	28.357251418955382	28.08130432747877	21.853314405803445
78-79	21.70533643228492	28.372411532581353	28.04601390639349	21.876238128740237
80-81	21.727185058328853	28.3417075246351	28.054543411204612	21.87656400583143
82-83	21.76304746255411	28.277548720666907	28.167344997533256	21.79205881924573
84-85	21.802463483771827	28.26187408267127	28.142265446547256	21.79339698700965
86-87	21.641565142029748	28.290639579026355	28.22809960806665	21.83969567087725
88-89	21.710413848971807	28.25760481783067	28.188169624582482	21.84381170861504
90-91	21.76286608177028	28.222532278354294	28.298585766985628	21.7160158728898
92-93	21.75558282490972	28.171008483756626	28.268587366920105	21.804821324413545
94-95	21.517992831448137	28.20551469349705	28.430694248141368	21.845798226913445
96-97	21.561916155891495	28.337243424923525	28.541044993050633	21.559795426134347
98-99	22.574720248728504	30.316861519089837	28.82892553311566	18.279492699065997
100	21.953972608970144	30.997922374399582	22.681909302606567	24.366195714023707
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9258.0
1	8493.5
2	12766.0
3	18788.5
4	25207.0
5	41334.5
6	55669.0
7	65700.5
8	71735.5
9	69517.0
10	62598.5
11	55518.5
12	58139.5
13	74021.0
14	112230.5
15	169029.5
16	215690.5
17	239565.5
18	247520.0
19	245333.0
20	236677.5
21	230905.0
22	235268.0
23	259850.5
24	312208.0
25	389985.0
26	488640.5
27	588857.5
28	679886.5
29	775790.0
30	862927.0
31	945844.0
32	1043900.0
33	1136009.0
34	1224402.0
35	1312486.0
36	1404596.5
37	1486765.5
38	1540394.0
39	1583497.0
40	1632750.5
41	1672579.5
42	1693430.0
43	1715621.5
44	1732026.0
45	1740617.5
46	1737218.5
47	1714607.0
48	1684868.5
49	1641235.5
50	1590505.0
51	1525434.5
52	1438147.0
53	1345941.5
54	1252892.0
55	1153173.0
56	1052600.0
57	949588.0
58	840270.0
59	729695.5
60	611479.5
61	497236.5
62	396299.0
63	310164.0
64	236082.0
65	176180.0
66	133417.0
67	103971.0
68	81332.0
69	59930.0
70	43200.5
71	31741.0
72	22864.0
73	16272.5
74	11598.0
75	8302.0
76	5950.5
77	4100.0
78	2847.5
79	2075.5
80	1501.0
81	1084.0
82	823.0
83	584.0
84	427.0
85	327.0
86	251.5
87	199.5
88	142.5
89	93.5
90	66.0
91	52.0
92	40.0
93	25.5
94	13.0
95	8.5
96	7.5
97	6.5
98	3.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.4228041302414216E-6
2	0.13362249339107488
3	2.64085650196315E-4
4	0.0
5	0.0
6	5.57244949955527E-5
7	2.4228041302414216E-6
8	2.4228041302414216E-6
9	0.002371925243506352
10-11	4.4700736202954233E-4
12-13	1.1871740238182966E-4
14-15	6.590027234256667E-4
16-17	0.0012392643126184872
18-19	4.724468053970773E-5
20-21	0.0028918142720592943
22-23	4.362111210934795E-5
24-25	8.666749237150308E-4
26-27	0.00523716180372704
28-29	0.001372960193586659
30-31	0.006013825792793012
32-33	0.004944557909820872
34-35	4.161507773996395E-5
36-37	7.628795133320885E-5
38-39	0.0
40-41	0.0
42-43	1.2593375471439306E-5
44-45	0.0
46-47	0.0
48-49	2.547922953357813E-4
50-51	0.0
52-53	5.300406852604497E-6
54-55	3.343814005615788E-5
56-57	0.0
58-59	0.0
60-61	0.0
62-63	2.778455831145951E-4
64-65	0.0
66-67	0.0
68-69	2.890883013567463E-6
70-71	2.92874137924975E-6
72-73	4.451689882996087E-6
74-75	3.758609979431232E-5
76-77	7.38843377406331E-4
78-79	0.003922660807180512
80-81	2.97477088980016E-5
82-83	4.770035400817727E-6
84-85	9.689878037381095E-6
86-87	4.925311832574071E-6
88-89	6.690130179730849E-6
90-91	7.018368285178282E-5
92-93	4.771217657546835E-4
94-95	0.0
96-97	1.871782627936764E-6
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1682.0
20-21	5885.0
22-23	11903.0
24-25	23169.0
26-27	32361.0
28-29	51573.0
30-31	111327.0
32-33	140236.0
34-35	199777.0
36-37	264770.0
38-39	318822.0
40-41	323134.0
42-43	382954.0
44-45	426337.0
46-47	420854.0
48-49	386048.0
50-51	354714.0
52-53	345465.0
54-55	370755.0
56-57	396932.0
58-59	399451.0
60-61	398760.0
62-63	382975.0
64-65	399245.0
66-67	425236.0
68-69	441936.0
70-71	453763.0
72-73	438008.0
74-75	446162.0
76-77	406572.0
78-79	457387.0
80-81	488919.0
82-83	485703.0
84-85	496474.0
86-87	538904.0
88-89	664204.0
90-91	695045.0
92-93	754100.0
94-95	894743.0
96-97	1776933.0
98-99	2922789.0
100-101	2.2338481E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.17809576205302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.48714117161508	71.83217619739673
2	7.48477792648832	12.15200038548339
3	1.989580739444436	4.845311273788701
4	0.8065157414455386	2.618856483706765
5	0.42148921630538	1.710784598195541
6	0.2509508261986921	1.2223026120434248
7	0.14076663108854826	0.7999016941025432
8	0.10492608862778205	0.6814160056451002
9	0.07139386421419906	0.5216056151402936
>10	0.23480599710676964	2.9647276202537194
>50	0.005846061013250165	0.32418558482315396
>100	0.001744963474524909	0.23144645390877203
>500	2.7255085244584956E-5	0.01684162695105325
>1k	3.351789227446771E-5	0.07844384856081774
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2114020651207108E-5
2	0.0	1.9382433041931373E-5	0.0	2.4228041302414216E-6	1.2114020651207108E-5
3	0.0	2.1805237172172796E-5	0.0	9.691216520965686E-6	1.2114020651207108E-5
4	0.0	6.783851564675981E-5	0.0	1.2114020651207108E-5	1.4536824781448531E-5
5	0.0	7.510692803748407E-5	0.0	1.4536824781448531E-5	1.6959628911689953E-5
6	0.0	7.510692803748407E-5	0.0	1.4536824781448531E-5	3.3919257823379906E-5
7	0.0	7.510692803748407E-5	0.0	1.9382433041931373E-5	4.118767021410417E-5
8	2.4228041302414216E-6	8.237534042820834E-5	0.0	2.665084543265564E-5	4.361047434434559E-5
9	4.845608260482843E-6	1.574822684656924E-4	0.0	4.361047434434559E-5	7.995253629796691E-5
10-11	6.057010325603555E-6	1.574822684656924E-4	0.0	5.451309293043199E-5	8.84323507538119E-5
12-13	7.268412390724266E-6	1.574822684656924E-4	0.0	7.268412390724264E-5	9.812356727477759E-5
14-15	9.691216520965686E-6	2.3985760889390074E-4	0.0	9.448936107941545E-5	1.453682478144853E-4
16-17	9.691216520965686E-6	2.4954882541486644E-4	0.0	1.223516085771918E-4	1.7444189737738237E-4
18-19	9.691216520965686E-6	2.834680832382463E-4	0.0	1.4900245400984742E-4	1.8655591802858949E-4
20-21	9.691216520965686E-6	3.1981014519186766E-4	0.0	1.7080769118202023E-4	1.9140152628907233E-4
22-23	9.691216520965686E-6	3.634206195362133E-4	0.0	1.9624713454955518E-4	2.1684096965660725E-4
24-25	9.691216520965686E-6	4.0824249594567956E-4	0.0	2.338005985682972E-4	2.3743480476365932E-4
26-27	9.691216520965686E-6	4.3852754757369735E-4	0.0	2.9194789769409135E-4	2.3864620682878003E-4
28-29	1.2114020651207108E-5	4.4095035170393877E-4	0.0	6.481001048395804E-4	2.4106901095902146E-4
30-31	1.2114020651207108E-5	5.063660632204571E-4	0.0	0.0018255829121369112	2.459146192195043E-4
32-33	1.574822684656924E-5	5.124230735460606E-4	0.0	0.004525798115290976	2.8346808323824636E-4
34-35	2.1805237172172796E-5	5.86318599518424E-4	0.0	0.009308413468387542	2.9437070182433277E-4
36-37	2.1805237172172796E-5	6.602141254907874E-4	0.0	0.015642834866903738	2.992163100848156E-4
38-39	2.1805237172172796E-5	6.66271135816391E-4	0.0	0.025097827985170885	3.0648472247553983E-4
40-41	4.6033278474587016E-5	7.438008679841165E-4	0.0	0.034154269824013324	3.125417328011434E-4
42-43	4.6033278474587016E-5	7.607604968958065E-4	0.0	0.04601268463947997	3.1981014519186766E-4
44-45	5.935870119091483E-5	8.564612600403425E-4	0.0	0.05931993632483097	3.2707855758259193E-4
46-47	7.87411342328462E-5	9.388366004685509E-4	0.0	0.07325227147578427	3.3919257823379905E-4
48-49	8.722094868869118E-5	9.400480025336717E-4	0.0	0.08640688650093006	3.525180009501269E-4
50-51	9.933496933989829E-5	0.0010078865181804314	0.0	0.0992538054015352	3.6099781540597186E-4
52-53	9.933496933989829E-5	0.0010151549305711556	0.0	0.11354956117202472	3.9007146496886894E-4
54-55	1.0660338173062256E-4	0.0010200005388316387	0.0	0.127381349951573	4.215679186620074E-4
56-57	1.1629459825158825E-4	0.0010248461470921215	0.0	0.13904351763249007	4.3004773311785237E-4
58-59	1.1629459825158825E-4	0.0010369601677433283	0.0	0.15010482988910728	4.3973894963881806E-4
60-61	1.1750600031670895E-4	0.0010539197966550184	0.0	0.1641546710403773	4.4821876409466304E-4
62-63	1.1992880444695037E-4	0.0010696680235015876	0.0	0.17764726724169178	4.5064156822490446E-4
64-65	1.2840861890279535E-4	0.0012089792609904695	0.0	0.18864922079711804	4.518529702900252E-4
66-67	1.332542271632782E-4	0.0012198818795765558	0.0	0.19956153059972542	4.542757744202666E-4
68-69	1.3931123748888176E-4	0.0012586467456604186	1.2114020651207108E-6	0.21342481583296685	4.56698578550508E-4
70-71	1.429454436842439E-4	0.0012877203952233157	2.4228041302414216E-6	0.22342130567434296	4.7123540333195656E-4
72-73	1.4657964987960603E-4	0.0012962002096791607	2.4228041302414216E-6	0.2303553710950939	5.039432590902157E-4
74-75	1.5505946433545098E-4	0.001327696663372299	2.4228041302414216E-6	0.23593145480084454	5.039432590902157E-4
76-77	1.5505946433545098E-4	0.0013761527459771276	2.4228041302414216E-6	0.23989758516204973	5.148458776763022E-4
78-79	1.562708664005717E-4	0.0013906895707585761	2.4228041302414216E-6	0.2436044754813191	5.233256921321471E-4
80-81	1.574822684656924E-4	0.0013906895707585761	2.4228041302414216E-6	0.24453119806113643	5.354397127833542E-4
82-83	1.574822684656924E-4	0.0014040149934749038	2.4228041302414216E-6	0.24513811049576195	5.693589706067342E-4
84-85	1.574822684656924E-4	0.0014185518182563525	2.4228041302414216E-6	0.2454627662492143	5.74204578867217E-4
86-87	1.574822684656924E-4	0.0014415684574936459	2.4228041302414216E-6	0.24562388272387534	5.74204578867217E-4
88	1.574822684656924E-4	0.0015021385607496816	2.4228041302414216E-6	0.24563599674452655	5.74204578867217E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	46415	0.0	47.60017	1
GTATCAA	82605	0.0	34.52177	1
TCAACGC	101895	0.0	27.291777	4
ATCAACG	101985	0.0	27.27966	3
CAACGCA	103725	0.0	26.786348	5
AACGCAG	106425	0.0	26.157299	6
TATCAAC	108690	0.0	25.8481	2
ACGCAGA	123525	0.0	22.51619	7
CGCAGAG	124875	0.0	22.239653	8
GTACATG	108590	0.0	20.319208	1
TACATGG	106825	0.0	20.09669	2
ACATGGG	107790	0.0	19.291704	3
GCAGAGT	148620	0.0	18.38328	9
AGAGTAC	132480	0.0	18.143913	10-11
GAGTACT	89765	0.0	17.378904	12-13
CAGAGTA	144735	0.0	16.854803	10-11
CATGGGG	74865	0.0	16.854673	4
AGTACTT	99490	0.0	15.725868	12-13
GTGGTAT	22800	0.0	14.966655	1
GTACTTT	105300	0.0	14.806767	14-15
>>END_MODULE