FastQCFastQC Report
Fri 27 May 2016
SRR2138782_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138782_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41809191
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA468400.045.568941
GTATCAA807000.033.5884631
ATCAACG996850.026.4853943
TCAACGC997200.026.4679994
CAACGCA1010100.026.1095145
AACGCAG1033850.025.5856866
TATCAAC1062600.025.0805662
ACGCAGA1190000.022.1068177
CGCAGAG1204900.021.8197178
GTACATG1079350.019.4800511
TACATGG1069500.019.1602332
ACATGGG1062600.018.6767483
GCAGAGT1446400.017.9625369
AGAGTAC1303300.017.65412710-11
GAGTACT888100.017.04300312-13
CATGGGG738000.016.602154
CAGAGTA1416950.016.51674310-11
AGTACTT988800.015.25507612-13
GTACTTT1031500.014.67970614-15
TATACTG250700.014.1308725
Produced by FastQC (version 0.11.5)