##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138782_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41809191
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.417591361669736	31.0	31.0	34.0	30.0	34.0
2	31.552923016376948	31.0	31.0	34.0	30.0	34.0
3	31.589663765558154	31.0	31.0	34.0	30.0	34.0
4	35.40931624340686	37.0	35.0	37.0	33.0	37.0
5	35.233696605131634	37.0	35.0	37.0	32.0	37.0
6	35.24886128507007	37.0	35.0	37.0	32.0	37.0
7	35.15177545530599	37.0	35.0	37.0	32.0	37.0
8	35.147253172155374	37.0	35.0	37.0	32.0	37.0
9	36.67201496436513	39.0	35.0	39.0	32.0	39.0
10-11	36.554366263628495	38.5	35.0	39.0	32.0	39.0
12-13	36.4965694385237	38.0	35.0	39.0	32.0	39.0
14-15	37.55419377763134	39.0	36.0	41.0	32.0	41.0
16-17	37.53309940869222	39.0	36.0	41.0	32.0	41.0
18-19	37.54062707168862	39.0	36.0	41.0	32.0	41.0
20-21	37.52173325447262	39.0	36.0	41.0	32.0	41.0
22-23	37.430398017723974	39.0	36.0	41.0	32.0	41.0
24-25	37.31946938942988	39.0	36.0	41.0	31.0	41.0
26-27	37.10546973596104	39.0	36.0	41.0	31.0	41.0
28-29	36.9260978738841	39.0	36.0	40.0	30.5	41.0
30-31	36.76440258658461	39.0	35.5	40.0	30.0	41.0
32-33	36.5872973783048	38.0	35.0	40.0	30.0	41.0
34-35	36.45139082209863	38.0	35.0	40.0	30.0	41.0
36-37	36.29476507601814	38.0	35.0	40.0	30.0	41.0
38-39	36.134769376891626	38.0	35.0	40.0	29.5	41.0
40-41	35.9364257607572	38.0	34.0	40.0	29.0	41.0
42-43	35.74188497893291	38.0	34.0	40.0	28.0	41.0
44-45	35.94763696040748	38.0	34.0	40.0	29.0	41.0
46-47	35.934427257539845	38.0	34.0	40.0	29.0	41.0
48-49	36.2797138074675	38.0	35.0	40.0	30.0	41.0
50-51	36.34454025488303	38.0	35.0	40.0	29.5	41.0
52-53	36.27762597779976	38.0	34.5	40.0	29.0	41.0
54-55	36.157574643376506	38.0	34.0	40.0	29.0	41.0
56-57	36.04155440369395	38.0	34.0	40.0	28.5	41.0
58-59	35.87766595402336	38.0	34.0	40.0	28.0	41.0
60-61	35.68368907100185	38.0	34.0	40.0	28.0	41.0
62-63	35.470439212112026	37.0	34.0	40.0	28.0	41.0
64-65	35.24927865482549	37.0	33.0	40.0	28.0	41.0
66-67	34.97879961811586	36.5	33.0	39.5	28.0	41.0
68-69	34.71180800885995	36.0	33.0	39.0	27.5	41.0
70-71	34.461168832023596	36.0	32.0	39.0	27.0	40.0
72-73	34.14511757070794	35.0	32.0	38.5	26.0	40.0
74-75	33.83087004570619	35.0	32.0	38.0	26.0	40.0
76-77	31.964727889306857	33.5	29.5	35.5	25.0	38.5
78-79	33.09453481242951	34.5	31.0	36.0	26.0	39.0
80-81	33.17699247935795	35.0	31.0	36.0	26.0	39.0
82-83	32.96159403572582	34.0	31.0	36.0	26.0	37.5
84-85	32.731238639236814	34.0	31.0	35.5	26.0	37.0
86-87	32.540177079705146	34.0	31.0	35.0	26.0	37.0
88-89	32.369930074593	34.0	31.0	35.0	26.0	36.0
90-91	32.195155496003665	34.0	31.0	35.0	26.0	36.0
92-93	32.16704122058218	34.0	31.0	35.0	26.0	36.0
94-95	32.17400521007263	34.0	31.0	35.0	26.0	35.0
96-97	32.27752198522059	34.0	31.0	35.0	26.5	35.0
98-99	32.42837178116518	34.0	31.0	35.0	27.0	35.0
100	30.95588601090913	32.0	29.0	34.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	1.0
15	0.0
16	0.0
17	0.0
18	13.0
19	115.0
20	660.0
21	4221.0
22	19162.0
23	61941.0
24	153299.0
25	306418.0
26	521714.0
27	779385.0
28	1063125.0
29	1343508.0
30	1626360.0
31	1928589.0
32	2272409.0
33	2701582.0
34	3279151.0
35	4057245.0
36	5273576.0
37	6836940.0
38	6651372.0
39	2844375.0
40	84028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.42040160499637	21.965930888258516	14.620177654238752	23.993489852506357
2	14.427740222543372	22.81297456544178	41.14936955400437	21.609915658010472
3	17.42738263685222	28.09506586105534	31.450839456445323	23.02671204564712
4	12.283873179942658	19.60376367961772	39.83999594730259	28.272367193137033
5	12.246166159971859	38.84769977969677	36.582202702750216	12.32393135758116
6	27.95990755042273	40.26919549632761	19.38889290050228	12.382004052747376
7	24.549563755012624	33.371334546989914	25.059903694381457	17.019198003615998
8	23.208432806078452	37.940339481813936	22.113419989398984	16.737807722708624
9	25.1134957701804	16.459203209248642	21.93150256818249	36.49579845238846
10-11	23.175888300356853	27.395275007020313	30.3108531773692	19.11798351525363
12-13	24.175470803842565	25.033651739050693	30.07075702982784	20.7201204272789
14-15	21.411346190770132	26.31633556772721	27.49251280424833	24.779805437254325
16-17	21.015891915267357	29.284393814872896	28.151712754384974	21.548001515474777
18-19	21.057871300910094	28.64662533713598	29.616905325240978	20.678598036712952
20-21	21.832259733087316	27.911375809075306	29.351940570358682	20.904423887478696
22-23	21.517053574819606	27.93069655429598	29.233724782088476	21.318525088795937
24-25	21.381457911981023	28.03181600151219	29.284781133896015	21.301944952610775
26-27	21.42711703542056	28.13419696893314	29.14075506406198	21.297930931584315
28-29	21.356217644802783	28.21203017888509	29.13110767359497	21.30064450271715
30-31	21.64933244517125	28.052167262245188	29.08107251093065	21.21742778165291
32-33	21.38228327600308	28.207649803703518	29.07049921180353	21.33956770848988
34-35	21.472953039749456	28.252331562850713	29.03648315000288	21.238232247396947
36-37	21.53283102544316	28.198530694043917	28.997961774689518	21.27067650582341
38-39	21.422953656559386	28.253798971484358	29.008605952962856	21.314641418993396
40-41	21.677857226122494	28.128955533459532	28.890160737276126	21.303026503141847
42-43	21.54611762860238	28.293706944434643	28.693232119709002	21.46694330725398
44-45	21.57566406367811	28.436136857856827	28.51981954260469	21.468379535860375
46-47	21.697509708194897	28.319033273345756	28.289062954807516	21.69439406365183
48-49	21.651767411944768	28.379383057887747	28.31457331253268	21.654276217634806
50-51	21.583161309500998	28.530176329175415	28.18391643629456	21.702745925029028
52-53	21.684136282606588	28.447635296456387	28.19011525759479	21.67811316334224
54-55	21.71276937830939	28.379299032719413	28.19117056346717	21.716761025504027
56-57	21.75394291698038	28.46420983691293	28.10520696697437	21.676640279132318
58-59	21.646447937854045	28.54918777564295	28.183951076485876	21.62041321001713
60-61	21.788910865726983	28.56142832279005	28.08317463296649	21.566486178516474
62-63	21.686932905759043	28.611566527955596	28.06429999312654	21.637200573158825
64-65	21.656173270893454	28.542876428216395	28.09368886618366	21.707261434706492
66-67	21.78612068143151	28.532126593859587	27.94385393437998	21.737898790328927
68-69	21.689397382915292	28.502400935266024	28.04210325282761	21.766098428991075
70-71	21.721839222209066	28.279621625530353	28.10924437907539	21.889294773185192
72-73	21.739687902384663	28.33556493168071	28.090273894563968	21.834473271370662
74-75	21.595815909143354	28.43003741127543	28.10854213349781	21.865604546083404
76-77	21.689635942399754	28.40159977934078	28.084326871944782	21.824437406314683
78-79	21.661885770999366	28.40090414056216	28.058106318129134	21.87910377030934
80-81	21.684755497903183	28.36802091828643	28.084106054394827	21.863117529415565
82-83	21.700892258380986	28.3061015497801	28.228899276775156	21.76410691506376
84-85	21.823502678561734	28.304964169805547	28.12611021226013	21.74542293937259
86-87	21.621364410848802	28.3282705869686	28.223027327725813	21.827337674456782
88-89	21.69763737840878	28.27743478594695	28.18814381126577	21.8367840243785
90-91	21.703351209232995	28.262502954561796	28.319993194369346	21.714152641835856
92-93	21.719935268832856	28.196896268203993	28.3165082074881	21.766660255475053
94-95	21.499417773157052	28.245592498893622	28.430469421686084	21.824520306263242
96-97	21.494035618053857	28.39501247404048	28.567793958903902	21.54315794900176
98-99	22.539867489731396	30.34482308274859	28.865867486270698	18.249441941249316
100	21.92211291706866	30.999036635888494	22.6496072862001	24.429243160842745
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7133.0
1	6907.0
2	11562.5
3	17409.0
4	23683.5
5	39405.5
6	53272.0
7	62646.5
8	67943.0
9	65538.5
10	59029.0
11	52157.0
12	54609.5
13	70075.5
14	107119.5
15	162464.5
16	208234.5
17	231631.5
18	239622.5
19	238143.5
20	230575.5
21	225335.0
22	229345.0
23	253293.0
24	303046.5
25	377149.0
26	474184.5
27	577002.5
28	676242.5
29	781413.0
30	880966.5
31	977002.5
32	1081093.5
33	1178746.0
34	1276524.0
35	1372925.5
36	1465833.5
37	1548202.5
38	1602422.0
39	1635235.5
40	1667497.0
41	1697000.5
42	1717558.5
43	1737350.5
44	1749787.0
45	1758638.0
46	1755184.5
47	1741466.0
48	1728847.5
49	1684464.5
50	1618644.0
51	1549253.5
52	1466392.5
53	1375778.0
54	1277363.5
55	1167125.0
56	1056951.5
57	946433.0
58	831971.5
59	719208.0
60	602035.5
61	489167.0
62	389126.0
63	302047.0
64	227480.0
65	170596.5
66	128316.0
67	97351.5
68	74030.0
69	53523.0
70	38249.5
71	27951.0
72	19871.0
73	13919.0
74	9707.0
75	6714.0
76	4718.5
77	3350.5
78	2413.5
79	1773.5
80	1309.0
81	946.5
82	703.0
83	493.5
84	338.5
85	246.0
86	196.5
87	161.0
88	118.5
89	80.5
90	55.0
91	36.5
92	28.0
93	20.5
94	16.5
95	14.5
96	12.5
97	11.0
98	5.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.13045935282507617
3	2.702754999492815E-4
4	0.0
5	0.0
6	5.2620008839683117E-5
7	0.0
8	0.0
9	0.002451614048212509
10-11	4.2215598000927595E-4
12-13	1.4111729643369564E-4
14-15	7.498351259654845E-4
16-17	0.0012222192962308214
18-19	5.2620008839683117E-5
20-21	0.002827324121423381
22-23	3.827860766397296E-5
24-25	8.077358163475366E-4
26-27	0.005011092955295364
28-29	0.001299098648446049
30-31	0.005927184644669121
32-33	0.00487125854663937
34-35	4.7116413288892E-5
36-37	8.015131596923889E-5
38-39	1.2228307789050539E-6
40-41	0.0
42-43	1.3669812368031166E-5
44-45	0.0
46-47	0.0
48-49	2.5382844443083854E-4
50-51	0.0
52-53	7.840901009434982E-6
54-55	3.5616138754144824E-5
56-57	0.0
58-59	0.0
60-61	0.0
62-63	3.142459385469739E-4
64-65	4.181042775760851E-6
66-67	0.0
68-69	4.28465530255158E-6
70-71	7.2355009099655365E-6
72-73	7.332582584421233E-6
74-75	3.863249353176904E-5
76-77	7.061165608136716E-4
78-79	0.004075149850595886
80-81	3.869123762482045E-5
82-83	6.287981767871104E-6
84-85	6.3874128110167035E-6
86-87	1.6234729511953997E-6
88-89	1.6540266227824735E-6
90-91	7.958409285343652E-5
92-93	5.814122099931083E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1719.0
20-21	6111.0
22-23	12141.0
24-25	24356.0
26-27	32557.0
28-29	51018.0
30-31	110188.0
32-33	138775.0
34-35	199360.0
36-37	264389.0
38-39	322808.0
40-41	325551.0
42-43	379028.0
44-45	421052.0
46-47	417887.0
48-49	390902.0
50-51	363043.0
52-53	351476.0
54-55	377675.0
56-57	409362.0
58-59	415374.0
60-61	417477.0
62-63	399640.0
64-65	414776.0
66-67	443154.0
68-69	451510.0
70-71	462607.0
72-73	444163.0
74-75	451161.0
76-77	411760.0
78-79	467303.0
80-81	500444.0
82-83	495189.0
84-85	504569.0
86-87	546322.0
88-89	678536.0
90-91	710297.0
92-93	767917.0
94-95	914239.0
96-97	1807492.0
98-99	2976479.0
100-101	2.2529384E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.76600578304439
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.67769770524703	69.93699742200752
2	7.80701761912511	12.454692251109261
3	2.1722337042829327	5.19811218653869
4	0.9198868794046924	2.9350280856936544
5	0.47813310517378804	1.9069384016178672
6	0.2863571572677412	1.3704939997580878
7	0.17421084066578557	0.9727272046811253
8	0.1185234410038976	0.7563313184434572
9	0.08127949916871915	0.5835006900661504
>10	0.27832960864612427	3.36433825086168
>50	0.004801640793882071	0.25369176182618464
>100	0.0014539614747614183	0.17814658531890634
>500	3.477381309128488E-5	0.01682953531582584
>1k	4.006393247781926E-5	0.07217230676173814
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.56727433448784E-6	0.0	4.78363716724392E-6	0.0
3	0.0	9.56727433448784E-6	0.0	2.152636725259764E-5	0.0
4	0.0	3.1093641587085484E-5	0.0	2.631000441984156E-5	2.39181858362196E-6
5	0.0	3.1093641587085484E-5	0.0	3.348546017070744E-5	4.78363716724392E-6
6	4.544455308881724E-5	3.1093641587085484E-5	0.0	3.348546017070744E-5	4.78363716724392E-6
7	4.544455308881724E-5	3.1093641587085484E-5	0.0	4.066091592157332E-5	4.78363716724392E-6
8	4.78363716724392E-5	3.58772787543294E-5	0.0	4.78363716724392E-5	7.17545575086588E-6
9	4.78363716724392E-5	7.653819467590272E-5	0.0	8.37136504267686E-5	1.19590929181098E-5
10-11	4.9032280964250184E-5	7.653819467590272E-5	0.0	1.1600320130566506E-4	1.19590929181098E-5
12-13	5.0228190256061164E-5	7.653819467590272E-5	0.0	1.5068457076818347E-4	1.19590929181098E-5
14-15	5.0228190256061164E-5	1.255704756401529E-4	0.0	1.865618495225129E-4	1.79386393771647E-5
16-17	7.893001325952468E-5	1.3035411280739684E-4	0.0	2.1645958181778737E-4	1.913454866897568E-5
18-19	7.893001325952468E-5	1.5546820793542742E-4	0.0	2.3559413048676307E-4	2.631000441984156E-5
20-21	7.893001325952468E-5	1.79386393771647E-4	0.0	2.810386835755803E-4	2.631000441984156E-5
22-23	8.490955971857957E-5	2.152636725259764E-4	0.0	3.408341481661293E-4	3.468136946251842E-5
24-25	9.088910617763448E-5	2.5592458844754973E-4	0.0	3.9345415700581244E-4	4.664046238062822E-5
26-27	9.328092476125644E-5	2.7266731853290343E-4	0.0	4.855391724752579E-4	4.9032280964250184E-5
28-29	9.56727433448784E-5	2.7386322782471445E-4	0.0	9.746660728259487E-4	5.0228190256061164E-5
30-31	9.56727433448784E-5	3.061527787036109E-4	0.0	0.0024862954176750273	5.0228190256061164E-5
32-33	9.806456192850037E-5	3.1332823445447675E-4	0.0	0.005502378651622319	5.262000883968312E-5
34-35	1.0284819909574429E-4	3.468136946251842E-4	0.0	0.010454639029011586	5.740364600692704E-5
36-37	1.0524001767936625E-4	3.779073362122697E-4	0.0	0.01731676654542299	5.740364600692704E-5
38-39	1.0524001767936625E-4	3.898664291303795E-4	0.0	0.028097888811098974	5.740364600692704E-5
40-41	1.0524001767936625E-4	4.18568252133843E-4	0.0	0.03853339329144159	5.740364600692704E-5
42-43	1.0882774555479919E-4	4.2933143576014186E-4	0.0	0.051172958596591836	5.979546459054901E-5
44-45	1.1002365484661017E-4	4.7238417026533716E-4	0.0	0.06517944822228203	6.218728317417097E-5
46-47	1.1002365484661017E-4	5.046737211442336E-4	0.0	0.07997523798056748	6.457910175779293E-5
48-49	1.1121956413842114E-4	5.058696304360446E-4	0.0	0.09300108198697268	7.414637609228076E-5
50-51	1.2796229422377487E-4	5.608814578593497E-4	0.0	0.10544451816826592	7.414637609228076E-5
52-53	1.315500220992078E-4	5.644691857347825E-4	0.0	0.11540883438763501	8.251774113495763E-5
54-55	1.315500220992078E-4	5.680569136102154E-4	0.0	0.1308001869732423	9.088910617763448E-5
56-57	1.3394184068282977E-4	5.716446414856484E-4	0.0	0.14652161052338947	9.208501546944546E-5
58-59	1.3633365926645172E-4	5.740364600692704E-4	0.0	0.15938122313823294	9.806456192850037E-5
60-61	1.494886614763725E-4	5.847996436955693E-4	0.0	0.17356351142981935	1.0045638051212233E-4
62-63	1.6025184510267134E-4	6.051301016563558E-4	0.0	0.18752575241171254	1.0524001767936625E-4
64-65	1.6025184510267134E-4	6.673173848305269E-4	0.0	0.19868836974147622	1.0524001767936625E-4
66-67	1.6503548226991525E-4	6.840601149158806E-4	0.0	0.20960702157571048	1.0882774555479919E-4
68-69	1.6503548226991525E-4	6.948232985421794E-4	0.0	0.22382877487392663	1.1361138272204311E-4
70-71	1.7699457518802506E-4	7.008028450012343E-4	0.0	0.23440539665070295	1.1719911059747604E-4
72-73	2.152636725259764E-4	7.019987542930452E-4	0.0	0.2417733937975504	1.2198274776471996E-4
74-75	2.152636725259764E-4	7.18741484378399E-4	0.0	0.24742884883852453	1.2198274776471996E-4
76-77	2.2124321898503132E-4	7.510310352572954E-4	0.0	0.25160376817623664	1.2198274776471996E-4
78-79	2.2483094686046425E-4	7.653819467590272E-4	0.0	0.2553266338016442	1.2198274776471996E-4
80-81	2.2483094686046425E-4	7.653819467590272E-4	0.0	0.2562761857793422	1.2317865705653095E-4
82-83	2.2483094686046425E-4	7.665778560508381E-4	0.0	0.25687892406241486	1.2676638493196388E-4
84-85	2.2483094686046425E-4	7.725574025098931E-4	0.0	0.25720062366191204	1.2676638493196388E-4
86-87	2.2483094686046425E-4	7.869083140116249E-4	0.0	0.25736446323489015	1.2676638493196388E-4
88	2.320064026113301E-4	8.203937741823323E-4	0.0	0.25738838142072634	1.2676638493196388E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	46840	0.0	45.56894	1
GTATCAA	80700	0.0	33.588463	1
ATCAACG	99685	0.0	26.485394	3
TCAACGC	99720	0.0	26.467999	4
CAACGCA	101010	0.0	26.109514	5
AACGCAG	103385	0.0	25.585686	6
TATCAAC	106260	0.0	25.080566	2
ACGCAGA	119000	0.0	22.106817	7
CGCAGAG	120490	0.0	21.819717	8
GTACATG	107935	0.0	19.480051	1
TACATGG	106950	0.0	19.160233	2
ACATGGG	106260	0.0	18.676748	3
GCAGAGT	144640	0.0	17.962536	9
AGAGTAC	130330	0.0	17.654127	10-11
GAGTACT	88810	0.0	17.043003	12-13
CATGGGG	73800	0.0	16.60215	4
CAGAGTA	141695	0.0	16.516743	10-11
AGTACTT	98880	0.0	15.255076	12-13
GTACTTT	103150	0.0	14.679706	14-15
TATACTG	25070	0.0	14.130872	5
>>END_MODULE