FastQCFastQC Report
Fri 27 May 2016
SRR2138781_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138781_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6882308
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108750.048.9480061
GTATCAA197400.036.7582931
CTTATAC117700.030.8066081
ATCAACG233850.030.4345263
TCAACGC234850.030.3407974
CAACGCA239550.029.7733125
TATCAAC244450.029.3215352
AACGCAG244000.029.2450226
ACGCAGA276800.025.7491517
TATACAC152950.025.6065253
CGCAGAG278850.025.5447568
TTATACA153000.024.6623122
GCAGAGT322100.021.9970999
GTGGTAT52500.021.577951
TGGTATC52150.020.5114612
ACACATC178600.020.3423616
GAGTACT208100.020.34145512-13
TACACAT194600.020.272475
CAGAGTA315850.019.56001910-11
GTACATG206900.018.6038551
Produced by FastQC (version 0.11.5)