##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138781_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6882308
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83342535672626	33.0	33.0	33.0	33.0	33.0
2	32.8342243619437	33.0	33.0	33.0	33.0	33.0
3	32.831675652993155	33.0	33.0	33.0	33.0	33.0
4	36.78058784930869	37.0	37.0	37.0	37.0	37.0
5	36.791141140443	37.0	37.0	37.0	37.0	37.0
6	36.80167438016433	37.0	37.0	37.0	37.0	37.0
7	36.788062376749195	37.0	37.0	37.0	37.0	37.0
8	36.78510697283527	37.0	37.0	37.0	37.0	37.0
9	36.784888296193664	37.0	37.0	37.0	37.0	37.0
10-11	36.76618948178431	37.0	37.0	37.0	37.0	37.0
12-13	36.75206544083758	37.0	37.0	37.0	37.0	37.0
14-15	39.10569419444756	40.0	40.0	40.0	37.0	40.0
16-17	39.06269837095347	40.0	40.0	40.0	37.0	40.0
18-19	39.04854788538961	40.0	40.0	40.0	37.0	40.0
20-21	39.10607562470335	40.0	40.0	40.0	37.0	40.0
22-23	39.13523456118416	40.0	40.0	40.0	37.0	40.0
24-25	39.14058528104505	40.0	40.0	40.0	37.0	40.0
26-27	38.878530919497706	40.0	40.0	40.0	37.0	40.0
28-29	38.88141973555666	40.0	40.0	40.0	37.0	40.0
30-31	38.90277593286915	40.0	40.0	40.0	37.0	40.0
32-33	38.884386248336114	40.0	40.0	40.0	37.0	40.0
34-35	38.85697139499974	40.0	40.0	40.0	37.0	40.0
36-37	38.82961868114631	40.0	40.0	40.0	37.0	40.0
38-39	38.78431220652426	40.0	37.0	40.0	37.0	40.0
40-41	38.75088470582338	40.0	37.0	40.0	37.0	40.0
42-43	38.70295816167939	40.0	37.0	40.0	37.0	40.0
44-45	38.639978241057705	40.0	37.0	40.0	37.0	40.0
46-47	38.57315707166046	40.0	37.0	40.0	37.0	40.0
48-49	38.53517482351895	40.0	37.0	40.0	37.0	40.0
50-51	38.48439318437854	40.0	37.0	40.0	37.0	40.0
52-53	38.438363314257025	40.0	37.0	40.0	37.0	40.0
54-55	38.366705150630615	40.0	37.0	40.0	37.0	40.0
56-57	38.2599316391896	40.0	37.0	40.0	37.0	40.0
58-59	38.14645228847856	40.0	37.0	40.0	37.0	40.0
60-61	38.000968522867794	40.0	37.0	40.0	37.0	40.0
62-63	37.834834773641326	40.0	37.0	40.0	33.0	40.0
64-65	37.64462464865535	38.5	37.0	40.0	33.0	40.0
66-67	37.43170431099013	37.0	37.0	40.0	33.0	40.0
68-69	37.19026722899209	37.0	37.0	40.0	33.0	40.0
70-71	36.94540028931969	37.0	37.0	40.0	33.0	40.0
72-73	36.70139546489864	37.0	37.0	40.0	33.0	40.0
74-75	36.428399192042136	37.0	37.0	37.0	33.0	40.0
76-77	34.759669876995545	37.0	33.0	37.0	30.0	37.0
78-79	31.442239502329723	37.0	33.0	37.0	8.5	37.0
80-81	34.61551862539808	37.0	33.0	37.0	30.0	37.0
82-83	35.65058260616363	37.0	35.0	37.0	33.0	37.0
84-85	35.759006529007195	37.0	37.0	37.0	33.0	37.0
86-87	35.713074424713724	37.0	37.0	37.0	33.0	37.0
88-89	35.63441818675974	37.0	37.0	37.0	33.0	37.0
90-91	35.567263276988925	37.0	37.0	37.0	33.0	37.0
92-93	35.49959077277964	37.0	35.0	37.0	33.0	37.0
94-95	35.45271572942814	37.0	33.0	37.0	33.0	37.0
96-97	35.43096281050381	37.0	33.0	37.0	33.0	37.0
98-99	35.398988558490075	37.0	33.0	37.0	33.0	37.0
100	35.34982479561349	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	0.0
13	1.0
14	0.0
15	1.0
16	0.0
17	2.0
18	1.0
19	9.0
20	28.0
21	96.0
22	373.0
23	1084.0
24	2767.0
25	5741.0
26	10762.0
27	17557.0
28	27051.0
29	38581.0
30	52161.0
31	69937.0
32	94307.0
33	129835.0
34	188451.0
35	302489.0
36	644177.0
37	2076316.0
38	3064578.0
39	155997.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.7869424036239	20.58581220137198	14.892126304140996	23.735119090863126
2	14.014237665620314	20.936813638680512	42.83603698061755	22.212911715081628
3	17.716269773232256	26.419209088145738	31.172282341153025	24.69223879746898
4	11.81846191856874	18.91824173588251	39.225712687028114	30.037583658520635
5	12.869606691656617	37.7301027180568	35.93322701414266	13.467063576143925
6	28.75304331047085	38.41442434718121	19.2575804512091	13.574951891138845
7	25.50779476884789	33.23694609424629	22.954102025076473	18.301157111829344
8	23.70183083930565	36.8695792167395	21.70674430728761	17.721845636667236
9	24.814379129791924	17.221490232637073	21.387854190774373	36.576276446796626
10-11	23.374020995138622	27.17053836958026	29.17210543120894	20.28333520407218
12-13	24.58609088695246	24.6808628733268	29.07854458126547	21.654501658455274
14-15	21.80007214150196	26.189726338150393	26.94319456083588	25.06700695951176
16-17	21.161180316340122	29.52169748300261	27.60585021811362	21.711271982543646
18-19	21.069174759397573	28.127838800588407	29.58220556243632	21.220780877577695
20-21	21.778459539539092	27.14879370524077	29.767466494395407	21.30528026082473
22-23	21.279297979050856	27.522328324530655	29.667804867983083	21.530568828435406
24-25	21.20852612129042	27.724422333828315	29.87243580873264	21.194615736148627
26-27	21.16476378064428	27.750314084925186	29.75516285505197	21.32975927937856
28-29	20.640798031211688	27.525552411593914	29.946098801008386	21.887550756186016
30-31	21.05378583633159	27.33079279876995	30.089290781965932	21.526130582932538
32-33	20.900138425338056	27.61433667226188	30.036334826396054	21.449190076004008
34-35	20.986534344360226	28.117445951915816	30.103773609762396	20.792246093961563
36-37	21.15317775543633	27.82817995850802	29.456527387212834	21.562114898842815
38-39	21.15574661107777	27.636816672470626	29.732288763531905	21.4751479529197
40-41	21.322042007274653	27.551577160846335	29.737433141750834	21.388947690128173
42-43	21.447976823377182	27.532642246672445	29.686290425028417	21.33309050492196
44-45	21.309006279863947	27.936309907939936	29.300451661757627	21.454232150438486
46-47	21.720161701493847	27.613245468138047	28.674922328669556	21.991670501698554
48-49	21.537336467781987	27.74300955659264	28.87755944903075	21.842094526594625
50-51	21.3861376434778	28.127855671392552	28.761139938126924	21.72486674700272
52-53	21.414881697628687	28.239286425963734	28.920963187715955	21.424868688691625
54-55	21.41572838585311	28.247036261859904	28.58020616218417	21.75702919010282
56-57	21.759857796337883	28.48438376888851	28.096754412312848	21.65900402246076
58-59	21.278711105964813	28.715057968471115	28.71097356266028	21.29525736290379
60-61	21.731711507571816	29.046443929013112	28.032316572089876	21.189527991325193
62-63	21.284478155127292	29.27968045441014	28.036714946435005	21.399126444027562
64-65	21.308190671620178	29.05950834457251	28.104065134828765	21.528235848978543
66-67	21.466688046152882	28.869795913033077	28.014951566080875	21.648564474733167
68-69	21.57059867591668	28.62763717473809	28.095241771220802	21.70652237812442
70-71	21.5250899795338	28.249111392361005	28.251434661651835	21.974363966453364
72-73	21.61671597854607	28.184151376291684	28.211342214000545	21.987790431161706
74-75	21.622018153770483	28.266180078919717	28.123104906771896	21.988696860537907
76-77	21.632420891046664	28.22095123286995	28.042525124216667	22.10410275186672
78-79	22.231913229691667	28.176786614302706	27.634176704254287	21.957123451751336
80-81	21.647498456969306	28.22258860339276	28.005611706974786	22.124301232663147
82-83	21.761831249805407	28.185534787296067	28.086466567743685	21.96616739515484
84-85	21.95325543336294	28.1816130507252	27.988756482227974	21.876375033683885
86-87	21.700961060285685	28.17349102461339	28.15231560650536	21.973232308595563
88-89	21.802702199016988	28.086761290535044	28.152344103758935	21.958192406689037
90-91	21.829592983140266	27.976879269238015	28.19976697544156	21.993760772180163
92-93	21.81066190117871	27.928396978813474	28.30220870020869	21.958732419799126
94-95	21.60894122532862	28.13652669278098	28.30294479801513	21.951587283875266
96-97	21.645810961975855	28.226554513129372	28.449597954453353	21.67803657044142
98-99	22.78411619127499	30.058250927795555	28.690373750478347	18.467259130451104
100	21.75812705606384	31.138635341955055	22.4192609369588	24.6839766650223
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1244.0
1	1138.5
2	1653.0
3	2492.0
4	3710.0
5	6175.0
6	8144.0
7	9538.5
8	10437.0
9	10437.5
10	9874.5
11	9350.0
12	10578.0
13	14407.0
14	22721.0
15	33608.5
16	42526.5
17	48105.5
18	49479.0
19	47119.5
20	42823.5
21	38057.0
22	35168.0
23	36011.0
24	40492.5
25	48526.0
26	59681.0
27	73038.0
28	87615.5
29	104145.5
30	121179.0
31	137735.0
32	155662.5
33	173432.5
34	191676.0
35	209376.5
36	226682.5
37	242266.0
38	252350.0
39	261042.5
40	271699.0
41	278703.5
42	281910.5
43	287375.0
44	292556.5
45	302277.0
46	314671.5
47	320909.0
48	311975.0
49	296781.5
50	284390.5
51	263150.0
52	238463.0
53	216797.5
54	196785.5
55	177636.0
56	159161.5
57	140099.5
58	121139.5
59	103601.5
60	86710.0
61	70345.0
62	55229.0
63	42350.5
64	31792.5
65	23237.5
66	16769.0
67	12413.0
68	9363.5
69	6721.5
70	4555.5
71	3168.0
72	2169.0
73	1450.5
74	982.0
75	621.5
76	394.0
77	256.5
78	172.0
79	116.0
80	75.5
81	46.5
82	31.5
83	21.5
84	16.0
85	11.5
86	8.0
87	6.5
88	4.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	3.4872022583121826E-4
4	0.02409075560117333
5	0.016593270745802136
6	0.0
7	0.0
8	0.0
9	0.0
10-11	7.265004704817046E-6
12-13	0.0
14-15	7.265004704817046E-6
16-17	7.265004704817046E-6
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008945743087124343
26-27	0.052527162780121844
28-29	0.08429325909185086
30-31	0.0750332775425313
32-33	0.03678990628570361
34-35	0.004266752694549469
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.494703493041468E-6
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.996832426988684E-4
56-57	6.202921910223203E-4
58-59	0.0
60-61	0.0
62-63	8.182733532146483E-6
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.013956938216363866
74-75	0.029326043176421686
76-77	0.0010398094575604854
78-79	5.133304874694335
80-81	0.0
82-83	0.01290606805745433
84-85	0.05139221303501901
86-87	0.041102483178616714
88-89	0.010121743431952043
90-91	9.464847131834247E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	77.0
20-21	405.0
22-23	960.0
24-25	1927.0
26-27	2385.0
28-29	5672.0
30-31	14206.0
32-33	19002.0
34-35	26073.0
36-37	37598.0
38-39	42443.0
40-41	47424.0
42-43	55294.0
44-45	66145.0
46-47	64896.0
48-49	56841.0
50-51	47329.0
52-53	42001.0
54-55	49362.0
56-57	56732.0
58-59	60270.0
60-61	59534.0
62-63	66193.0
64-65	70201.0
66-67	77808.0
68-69	65183.0
70-71	55074.0
72-73	51358.0
74-75	52833.0
76-77	54414.0
78-79	55033.0
80-81	55239.0
82-83	53209.0
84-85	53111.0
86-87	56254.0
88-89	60911.0
90-91	63613.0
92-93	65628.0
94-95	71357.0
96-97	209135.0
98-99	411265.0
100-101	4477913.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.85787634501611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.75313799257064	78.82624837680885
2	6.742290722658462	11.712421077416359
3	1.4624608835137416	3.8107873973897872
4	0.5005498198538915	1.7390677742955756
5	0.22579507529451778	0.9806040364622416
6	0.10746916783665303	0.5600726214514674
7	0.05918219756450729	0.3598307998519001
8	0.037260308270271325	0.2589080998653127
9	0.02310403775967009	0.18060908893200073
>10	0.08560659616502934	1.1303350320937626
>50	0.0017348506733313772	0.09745486182699024
>100	0.0012321265086125382	0.22737747388355284
>500	1.51046932675651E-4	0.08313458328804742
>1k	2.517439803508801E-5	0.03314877643413307
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4530009409634094E-5	0.0
2	0.0	1.4530009409634094E-5	0.0	1.4530009409634094E-5	0.0
3	0.0	1.4530009409634094E-5	0.0	1.4530009409634094E-5	0.0
4	0.0	4.359002822890228E-5	0.0	1.4530009409634094E-5	0.0
5	0.0	4.359002822890228E-5	0.0	1.4530009409634094E-5	0.0
6	0.0	4.359002822890228E-5	0.0	1.4530009409634094E-5	1.4530009409634094E-5
7	0.0	4.359002822890228E-5	0.0	1.4530009409634094E-5	1.4530009409634094E-5
8	0.0	7.265004704817047E-5	0.0	1.4530009409634094E-5	2.906001881926819E-5
9	0.0	1.1624007527707275E-4	0.0	2.906001881926819E-5	2.906001881926819E-5
10-11	0.0	1.1624007527707275E-4	0.0	1.0171006586743866E-4	2.906001881926819E-5
12-13	0.0	1.1624007527707275E-4	0.0	1.0171006586743866E-4	2.906001881926819E-5
14-15	0.0	1.5256509880115797E-4	0.0	1.380350893915239E-4	5.085503293371933E-5
16-17	0.0	1.5983010350597502E-4	0.0	1.5983010350597502E-4	7.265004704817047E-5
18-19	0.0	1.5983010350597502E-4	0.0	2.1068513643969435E-4	7.265004704817047E-5
20-21	0.0	1.7436011291560913E-4	0.0	3.6325023524085237E-4	7.265004704817047E-5
22-23	0.0	2.2521514584932846E-4	0.0	7.846205081202411E-4	7.265004704817047E-5
24-25	0.0	2.470101599637796E-4	0.0	0.0019833462844150536	7.265004704817047E-5
26-27	0.0	2.470101599637796E-4	0.0	0.005114563312191201	7.991505175298751E-5
28-29	0.0	2.470101599637796E-4	0.0	0.01517659482836281	8.718005645780457E-5
30-31	0.0	2.760701787830478E-4	0.0	0.016004805364711952	8.718005645780457E-5
32-33	0.0	2.906001881926819E-4	0.0	0.017755671498572864	1.0171006586743866E-4
34-35	0.0	3.269252117167671E-4	0.0	0.020538168300517794	1.1624007527707275E-4
36-37	0.0	3.632502352408523E-4	0.0	0.024497595864643083	1.1624007527707275E-4
38-39	0.0	3.632502352408523E-4	0.0	0.04998323236914128	1.1624007527707275E-4
40-41	0.0	3.777802446504864E-4	0.0	0.07688554479107881	1.3077008468670684E-4
42-43	0.0	3.850452493553035E-4	0.0	0.08392533435004652	1.4530009409634092E-4
44-45	0.0	4.4316528699383986E-4	0.0	0.09400189587562777	1.5983010350597502E-4
46-47	0.0	4.7949031051792507E-4	0.0	0.10500111299872078	1.888901223252432E-4
48-49	0.0	4.7949031051792507E-4	0.0	0.11562981488186812	1.888901223252432E-4
50-51	0.0	5.812003763853638E-4	0.0	0.12626578176972025	1.888901223252432E-4
52-53	0.0	5.812003763853638E-4	0.0	0.20953726569633324	1.9615512703006027E-4
54-55	0.0	5.812003763853638E-4	0.0	0.31547556430197543	2.0342013173487732E-4
56-57	0.0	5.812003763853638E-4	0.0	0.42397840956841804	2.1068513643969435E-4
58-59	0.0	5.884653810901808E-4	0.0	0.5393684792950272	2.179501411445114E-4
60-61	0.0	6.10260395204632E-4	0.0	0.5758460679179136	2.179501411445114E-4
62-63	0.0	6.10260395204632E-4	0.0	0.6338280704670585	2.179501411445114E-4
64-65	0.0	6.538504234335342E-4	0.0	0.6409405100730743	2.179501411445114E-4
66-67	0.0	6.538504234335342E-4	0.0	0.6460405433758559	2.2521514584932846E-4
68-69	0.0	6.683804328431683E-4	0.0	0.6503341611564027	2.3974515525896256E-4
70-71	0.0	6.683804328431683E-4	0.0	0.6542354686828895	2.615401693734137E-4
72-73	0.0	6.756454375479854E-4	0.0	0.6576354908847439	3.05130197602316E-4
74-75	0.0	6.974404516624365E-4	0.0	0.6600474724467431	3.05130197602316E-4
76-77	0.0	7.337654751865217E-4	0.0	0.6622560338770075	3.05130197602316E-4
78-79	0.0	7.410304798913387E-4	0.0	0.6639778399920492	3.05130197602316E-4
80-81	0.0	7.410304798913387E-4	0.0	0.6647188704719404	3.12395202307133E-4
82-83	0.0	7.410304798913387E-4	0.0	0.6653146008577355	3.632502352408523E-4
84-85	0.0	7.410304798913387E-4	0.0	0.6654526359471271	3.632502352408523E-4
86-87	0.0	7.410304798913387E-4	0.0	0.6655180209894704	3.7051523994566937E-4
88	0.0	7.70090498710607E-4	0.0	0.6655180209894704	3.777802446504864E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10875	0.0	48.948006	1
GTATCAA	19740	0.0	36.758293	1
CTTATAC	11770	0.0	30.806608	1
ATCAACG	23385	0.0	30.434526	3
TCAACGC	23485	0.0	30.340797	4
CAACGCA	23955	0.0	29.773312	5
TATCAAC	24445	0.0	29.321535	2
AACGCAG	24400	0.0	29.245022	6
ACGCAGA	27680	0.0	25.749151	7
TATACAC	15295	0.0	25.606525	3
CGCAGAG	27885	0.0	25.544756	8
TTATACA	15300	0.0	24.662312	2
GCAGAGT	32210	0.0	21.997099	9
GTGGTAT	5250	0.0	21.57795	1
TGGTATC	5215	0.0	20.511461	2
ACACATC	17860	0.0	20.342361	6
GAGTACT	20810	0.0	20.341455	12-13
TACACAT	19460	0.0	20.27247	5
CAGAGTA	31585	0.0	19.560019	10-11
GTACATG	20690	0.0	18.603855	1
>>END_MODULE