FastQCFastQC Report
Fri 27 May 2016
SRR2138780_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138780_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6751003
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93700.050.930481
GTATCAA173650.038.1512641
TCAACGC207950.031.3064794
ATCAACG209200.031.0993373
TATCAAC214250.030.6439862
CAACGCA212950.030.503865
AACGCAG220050.029.5902926
ACGCAGA251950.025.8269357
CGCAGAG253400.025.6288628
GTGGTAT44250.022.8560561
GCAGAGT291800.022.154299
TGGTATC42450.022.023312
GTACATG192050.021.5296081
TACATGG193000.020.8951992
ACATGGG192900.020.5535563
CTTATAC103550.020.1907561
GAGTACT189100.020.05803712-13
CAGAGTA286850.019.4937110-11
AGAGTAC270200.018.61993610-11
GTACTTT207350.018.35420814-15
Produced by FastQC (version 0.11.5)