##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138780_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6751003
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.818973417727705	33.0	33.0	33.0	33.0	33.0
2	32.8176202854598	33.0	33.0	33.0	33.0	33.0
3	32.81600615493728	33.0	33.0	33.0	33.0	33.0
4	36.76228095291914	37.0	37.0	37.0	37.0	37.0
5	36.773591124163325	37.0	37.0	37.0	37.0	37.0
6	36.78438700145741	37.0	37.0	37.0	37.0	37.0
7	36.77049662102061	37.0	37.0	37.0	37.0	37.0
8	36.76524332754703	37.0	37.0	37.0	37.0	37.0
9	36.76665067398133	37.0	37.0	37.0	37.0	37.0
10-11	36.743085879238976	37.0	37.0	37.0	37.0	37.0
12-13	36.72912980486011	37.0	37.0	37.0	37.0	37.0
14-15	39.049353851568426	40.0	40.0	40.0	37.0	40.0
16-17	39.00192460290715	40.0	40.0	40.0	37.0	40.0
18-19	38.97902422795546	40.0	40.0	40.0	37.0	40.0
20-21	39.0334916805065	40.0	40.0	40.0	37.0	40.0
22-23	39.0668076619381	40.0	40.0	40.0	37.0	40.0
24-25	39.07170270866854	40.0	40.0	40.0	37.0	40.0
26-27	38.79786800952907	40.0	40.0	40.0	37.0	40.0
28-29	38.795405790059974	40.0	40.0	40.0	37.0	40.0
30-31	38.81671318818964	40.0	37.0	40.0	37.0	40.0
32-33	38.80249306733671	40.0	37.0	40.0	37.0	40.0
34-35	38.77355195239284	40.0	37.0	40.0	37.0	40.0
36-37	38.73832281632578	40.0	37.0	40.0	37.0	40.0
38-39	38.68875694144728	40.0	37.0	40.0	37.0	40.0
40-41	38.6500141645287	40.0	37.0	40.0	37.0	40.0
42-43	38.59494371115224	40.0	37.0	40.0	37.0	40.0
44-45	38.52717351034798	40.0	37.0	40.0	37.0	40.0
46-47	38.45170719629335	40.0	37.0	40.0	37.0	40.0
48-49	38.401352171149476	40.0	37.0	40.0	37.0	40.0
50-51	38.33555274363152	40.0	37.0	40.0	37.0	40.0
52-53	38.26687484521506	40.0	37.0	40.0	37.0	40.0
54-55	38.19541934125667	40.0	37.0	40.0	37.0	40.0
56-57	38.087004553741764	40.0	37.0	40.0	37.0	40.0
58-59	37.95229906034414	40.0	37.0	40.0	35.0	40.0
60-61	37.78918297785546	40.0	37.0	40.0	33.0	40.0
62-63	37.59987798450073	38.5	37.0	40.0	33.0	40.0
64-65	37.38659478850059	37.0	37.0	40.0	33.0	40.0
66-67	37.15841773704297	37.0	37.0	40.0	33.0	40.0
68-69	36.89945604277335	37.0	37.0	40.0	33.0	40.0
70-71	36.6431388031219	37.0	37.0	40.0	33.0	40.0
72-73	36.39594612448201	37.0	37.0	37.0	33.0	40.0
74-75	36.12713189317324	37.0	37.0	37.0	33.0	40.0
76-77	34.41085790135562	35.0	33.0	37.0	30.0	37.0
78-79	31.095077737451568	37.0	33.0	37.0	8.5	37.0
80-81	34.327195766035615	37.0	33.0	37.0	30.0	37.0
82-83	35.42579500843739	37.0	33.0	37.0	33.0	37.0
84-85	35.56319276688981	37.0	37.0	37.0	33.0	37.0
86-87	35.52825528539006	37.0	37.0	37.0	33.0	37.0
88-89	35.44257074992426	37.0	33.0	37.0	33.0	37.0
90-91	35.3662519338446	37.0	33.0	37.0	33.0	37.0
92-93	35.28647677128282	37.0	33.0	37.0	33.0	37.0
94-95	35.23290958439118	37.0	33.0	37.0	33.0	37.0
96-97	35.20262609266348	37.0	33.0	37.0	33.0	37.0
98-99	35.168008679361705	37.0	33.0	37.0	33.0	37.0
100	35.11439804265727	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	12.0
21	116.0
22	425.0
23	1301.0
24	3180.0
25	6342.0
26	11746.0
27	19463.0
28	29454.0
29	41521.0
30	57141.0
31	77167.0
32	103912.0
33	143018.0
34	209053.0
35	340039.0
36	744524.0
37	2194168.0
38	2647126.0
39	121293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53804997568509	20.1014278915296	14.508673748182307	23.851848384603
2	14.540965246201193	20.796554230534337	41.850462812710944	22.81201771055353
3	18.106870251622354	25.933900065027498	30.624619500251075	25.33461018309907
4	12.442431202277254	18.314304115558812	38.530710198764865	30.712554483399074
5	13.479091601217966	37.39812321378121	35.38139507252136	13.741390112479468
6	29.80903726453684	37.60568614767317	18.787000983409428	13.798275604380564
7	26.341182191742472	32.764079648609254	22.648190202255872	18.246547957392405
8	24.37763692298759	36.29364407036999	21.23352633675322	18.0951926698892
9	25.370437548316893	16.907354359048572	21.096968850406377	36.625239242228155
10-11	23.980703311789377	26.749328951564678	28.72862743506409	20.541340301581855
12-13	25.064349697370897	24.30095202149962	28.745180530952215	21.88951775017727
14-15	22.205218987460086	25.731109881005832	26.531383558857847	25.53228757267624
16-17	21.686229438795984	28.951090674970814	27.194899780077126	22.16778010615608
18-19	21.7259939004619	27.82233247415236	29.01739193420593	21.434281691179816
20-21	22.435598091652665	26.972763130071804	29.087246935301277	21.504391842974254
22-23	22.160718433646124	27.174256986894907	28.87520532262614	21.78981925683283
24-25	22.04046904312834	27.337775199132324	28.925521558909228	21.696234198830112
26-27	22.050168965121717	27.45918081889046	28.860549222468308	21.630100993519513
28-29	21.761555794584595	27.360052063641422	28.949529212147834	21.92886292962615
30-31	22.302967431632098	27.26305481918417	28.862894436345165	21.571083312838567
32-33	22.076773411767313	27.40102819979178	28.907228773226063	21.61496961521484
34-35	22.02840705431457	27.521170446651638	28.969700206099635	21.480722292934153
36-37	22.073623364132576	27.36880775045194	28.779580221036145	21.77798866437934
38-39	21.828684617068536	27.33825791305451	28.94742921722481	21.885628252652147
40-41	22.071882759038335	27.23337673348271	28.844629988493914	21.85011051898504
42-43	22.083749752815425	27.28801089098084	28.707282790962434	21.920956565241294
44-45	22.015879761091366	27.50872082280405	28.44806424739624	22.027335168708344
46-47	22.265336834274645	27.390752762358094	27.97958997541193	22.36432042795533
48-49	22.161628167654097	27.524160264292806	28.0245059279259	22.289705640127185
50-51	22.10906192591686	27.71688882260494	27.934232514846652	22.239816736631546
52-53	22.184053995834972	27.721328840559167	27.975837184505615	22.11877997910025
54-55	22.167184422865347	27.649626430537577	27.834720178868054	22.34846896772902
56-57	22.33277346392735	27.812925551952844	27.583447189173555	22.270853794946248
58-59	22.05652896416778	27.941862516400214	27.91967548262268	22.081933036809325
60-61	22.325913455522713	28.0627496629234	27.593488728883912	22.01784815266997
62-63	22.046899444637198	28.291113643369243	27.559491069370484	22.102495842623078
64-65	22.05833319270739	28.188064240812356	27.517590227198042	22.23601233928221
66-67	22.148785888577304	28.134840497638365	27.394190030770638	22.322183583013693
68-69	22.158653676781746	27.99479793394446	27.512995615001273	22.333552774272526
70-71	22.186316315373418	27.732811826773172	27.55633452793351	22.524537329919898
72-73	22.186808387350652	27.7117269830013	27.470943079657346	22.630521549990704
74-75	22.19084952173404	27.841626458517908	27.38483373467316	22.58269028507489
76-77	22.33268662442683	27.70180226796703	27.317561592620216	22.647949514985925
78-79	22.84406081826848	27.722434693018233	26.887781370599377	22.54572311811391
80-81	22.371497307183407	27.82775336393568	27.181296228837127	22.61945310004378
82-83	22.438733187657984	27.795548303076558	27.255056118631753	22.5106623906337
84-85	22.495573145029592	27.843146290424908	27.1870108161825	22.474269748363
86-87	22.324725818750128	27.888469088422973	27.26758016880772	22.51922492401917
88-89	22.426021953235253	27.913771343837553	27.21799878866356	22.442207914263633
90-91	22.510831325245555	27.903460207906015	27.204209212157476	22.38149925469095
92-93	22.55664565490164	27.87593884941276	27.2428871923594	22.3245283033262
94-95	22.394345374464823	28.00090076005492	27.302560648006473	22.30219321747378
96-97	22.482530718382705	28.001926248358057	27.455428474759476	22.06011455849977
98-99	23.593078407307043	29.89102921768924	27.62247626543634	18.893416109567372
100	22.55805845724621	30.781113675585956	21.547778217161433	25.113049650006396
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1617.0
1	1440.0
2	1872.0
3	2641.0
4	3625.0
5	5822.0
6	7636.0
7	8908.5
8	9818.0
9	9905.0
10	9486.0
11	9073.5
12	9959.0
13	12981.5
14	20207.5
15	30162.0
16	38328.0
17	43431.5
18	44794.0
19	42393.5
20	38182.0
21	33536.5
22	30167.5
23	30493.0
24	34493.5
25	41574.5
26	51411.5
27	62877.0
28	75863.0
29	90290.5
30	105115.0
31	119855.0
32	135524.0
33	150943.5
34	166174.5
35	180995.0
36	196536.5
37	210644.0
38	221766.0
39	232567.5
40	244354.0
41	254430.5
42	260607.5
43	266054.0
44	270983.5
45	276603.0
46	282713.5
47	289010.5
48	296565.0
49	294315.5
50	283028.5
51	272409.0
52	258742.0
53	241519.5
54	224011.5
55	207571.0
56	190328.5
57	171409.5
58	151178.5
59	130222.0
60	108262.0
61	87288.0
62	69351.0
63	53776.0
64	40180.5
65	29450.5
66	21889.5
67	16554.5
68	12412.0
69	8873.0
70	6259.5
71	4478.0
72	3086.0
73	2040.0
74	1335.0
75	869.5
76	575.5
77	369.0
78	242.0
79	161.5
80	111.0
81	72.0
82	46.5
83	29.5
84	20.0
85	19.0
86	15.0
87	9.5
88	8.5
89	6.5
90	5.0
91	5.5
92	5.0
93	3.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	1.9256397901171128E-4
4	0.021774542242093506
5	0.015701370596339536
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008689948418896112
26-27	0.052674439431842295
28-29	0.08534679149892276
30-31	0.07553645953481357
32-33	0.036123260866169904
34-35	0.004556746748321182
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	4.3122872155777064E-4
56-57	7.962070945912526E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	8.571019121600819E-6
72-73	0.014155650062991345
74-75	0.02954423632248838
76-77	0.0010737781526808676
78-79	5.278083021357021
80-81	0.0
82-83	0.012901043653691446
84-85	0.0508188775361005
86-87	0.03993242120474306
88-89	0.009816903103623705
90-91	2.827959926676655E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	74.0
20-21	393.0
22-23	881.0
24-25	1912.0
26-27	2979.0
28-29	5337.0
30-31	12769.0
32-33	17063.0
34-35	23240.0
36-37	33325.0
38-39	37546.0
40-41	42364.0
42-43	50725.0
44-45	60185.0
46-47	58885.0
48-49	51716.0
50-51	42890.0
52-53	37310.0
54-55	42564.0
56-57	46697.0
58-59	46879.0
60-61	50669.0
62-63	55570.0
64-65	59184.0
66-67	64572.0
68-69	58302.0
70-71	52439.0
72-73	49891.0
74-75	51234.0
76-77	53035.0
78-79	51698.0
80-81	53008.0
82-83	51501.0
84-85	51494.0
86-87	55134.0
88-89	58286.0
90-91	60495.0
92-93	63319.0
94-95	76590.0
96-97	218455.0
98-99	414311.0
100-101	4486082.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.43858858116641
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.12999373224933	79.68278029358424
2	6.508726611921589	11.382277368142022
3	1.362787830161853	3.574807332148283
4	0.46976676648560833	1.6430297209535993
5	0.21644547216398344	0.946284329540143
6	0.09897654034216254	0.5192621394099343
7	0.060297055727527096	0.36906026138904346
8	0.03509772100025559	0.2455116149342331
9	0.024926996448422084	0.1961623248315998
>10	0.09061539908306414	1.2302619947856726
>50	0.001785543930015105	0.10652345124221067
>100	5.552326992221363E-4	0.0845957654967833
>500	2.5097787007946517E-5	0.019443403542222234
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.4812613770131637E-5	0.0	1.4812613770131637E-5	1.4812613770131637E-5
5	0.0	1.4812613770131637E-5	0.0	1.4812613770131637E-5	1.4812613770131637E-5
6	0.0	1.4812613770131637E-5	0.0	4.4437841310394914E-5	2.9625227540263275E-5
7	0.0	2.9625227540263275E-5	0.0	5.925045508052655E-5	2.9625227540263275E-5
8	0.0	2.9625227540263275E-5	0.0	5.925045508052655E-5	4.4437841310394914E-5
9	0.0	5.925045508052655E-5	0.0	7.406306885065819E-5	4.4437841310394914E-5
10-11	0.0	5.925045508052655E-5	0.0	7.406306885065819E-5	4.4437841310394914E-5
12-13	0.0	5.925045508052655E-5	0.0	9.628198950585565E-5	5.184414819546073E-5
14-15	7.406306885065819E-6	8.1469375735724E-5	0.0	1.1109460327598727E-4	8.887568262078983E-5
16-17	1.4812613770131637E-5	8.887568262078983E-5	0.0	1.2590721704611892E-4	1.3331352393118474E-4
18-19	1.4812613770131637E-5	1.0368829639092146E-4	0.0	1.4071983081625057E-4	1.3331352393118474E-4
20-21	1.4812613770131637E-5	1.0368829639092146E-4	0.0	1.555324445863822E-4	1.3331352393118474E-4
22-23	1.4812613770131637E-5	1.1109460327598727E-4	0.0	1.6293875147144803E-4	1.555324445863822E-4
24-25	1.4812613770131637E-5	1.3331352393118474E-4	0.0	1.7775136524157966E-4	1.7775136524157966E-4
26-27	1.4812613770131637E-5	1.3331352393118474E-4	0.0	2.4440812720717204E-4	1.925639790117113E-4
28-29	1.4812613770131637E-5	1.3331352393118474E-4	0.0	5.628793232650023E-4	2.0737659278184293E-4
30-31	1.4812613770131637E-5	1.4812613770131638E-4	0.0	0.0018219514937261915	2.2218920655197458E-4
32-33	1.4812613770131637E-5	1.4812613770131638E-4	0.0	0.004740036406442124	2.2218920655197458E-4
34-35	1.4812613770131637E-5	1.925639790117113E-4	0.0	0.008369126780124376	2.6662704786236947E-4
36-37	1.4812613770131637E-5	2.295955134370404E-4	0.0	0.014871864225212163	2.6662704786236947E-4
38-39	1.4812613770131637E-5	2.4440812720717204E-4	0.0	0.04147531855636859	2.6662704786236947E-4
40-41	1.4812613770131637E-5	2.9625227540263277E-4	0.0	0.0687305278934108	2.6662704786236947E-4
42-43	1.4812613770131637E-5	3.036585822876986E-4	0.0	0.08001033327936605	2.8143966163250115E-4
44-45	1.4812613770131637E-5	3.480964235980935E-4	0.0	0.09374162624427806	2.9625227540263277E-4
46-47	1.4812613770131637E-5	3.851279580234226E-4	0.0	0.11023547167731965	3.036585822876986E-4
48-49	1.4812613770131637E-5	3.851279580234226E-4	0.0	0.1251962115851526	3.110648891727644E-4
50-51	1.4812613770131637E-5	4.8140994752927826E-4	0.0	0.14089758218149212	3.110648891727644E-4
52-53	1.4812613770131637E-5	4.888162544143441E-4	0.0	0.2309804928245477	3.25877502942896E-4
54-55	1.4812613770131637E-5	4.888162544143441E-4	0.0	0.34516352607160744	3.406901167130277E-4
56-57	1.4812613770131637E-5	5.036288681844757E-4	0.0	0.46429397231789116	3.406901167130277E-4
58-59	1.4812613770131637E-5	5.036288681844757E-4	0.0	0.580965524678333	3.406901167130277E-4
60-61	1.4812613770131637E-5	5.332540957247389E-4	0.0	0.6688857937109494	3.406901167130277E-4
62-63	1.4812613770131637E-5	5.332540957247389E-4	0.0	0.7274400559442797	3.555027304831593E-4
64-65	1.4812613770131637E-5	6.221297783455288E-4	0.0	0.7345056727126325	3.555027304831593E-4
66-67	1.4812613770131637E-5	6.295360852305946E-4	0.0	0.7407862209511682	3.555027304831593E-4
68-69	1.4812613770131637E-5	6.517550058857921E-4	0.0	0.7461187619084158	3.555027304831593E-4
70-71	1.4812613770131637E-5	6.59161312770858E-4	0.0	0.7504070135948688	3.555027304831593E-4
72-73	1.4812613770131637E-5	6.739739265409896E-4	0.0	0.7534880372590562	3.851279580234226E-4
74-75	1.4812613770131637E-5	6.813802334260554E-4	0.0	0.7557543671658863	3.851279580234226E-4
76-77	1.4812613770131637E-5	6.813802334260554E-4	0.0	0.7581910421310729	3.851279580234226E-4
78-79	1.4812613770131637E-5	6.813802334260554E-4	0.0	0.7598426485664427	3.851279580234226E-4
80-81	2.2218920655197457E-5	6.813802334260554E-4	0.0	0.7605980918687194	3.851279580234226E-4
82-83	2.9625227540263275E-5	6.813802334260554E-4	0.0	0.760998032440513	3.851279580234226E-4
84-85	2.9625227540263275E-5	6.887865403111212E-4	0.0	0.7611239396575591	3.851279580234226E-4
86-87	2.9625227540263275E-5	7.110054609663186E-4	0.0	0.7611461585782142	3.851279580234226E-4
88	2.9625227540263275E-5	7.554433022767136E-4	0.0	0.7611461585782142	3.851279580234226E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9370	0.0	50.93048	1
GTATCAA	17365	0.0	38.151264	1
TCAACGC	20795	0.0	31.306479	4
ATCAACG	20920	0.0	31.099337	3
TATCAAC	21425	0.0	30.643986	2
CAACGCA	21295	0.0	30.50386	5
AACGCAG	22005	0.0	29.590292	6
ACGCAGA	25195	0.0	25.826935	7
CGCAGAG	25340	0.0	25.628862	8
GTGGTAT	4425	0.0	22.856056	1
GCAGAGT	29180	0.0	22.15429	9
TGGTATC	4245	0.0	22.02331	2
GTACATG	19205	0.0	21.529608	1
TACATGG	19300	0.0	20.895199	2
ACATGGG	19290	0.0	20.553556	3
CTTATAC	10355	0.0	20.190756	1
GAGTACT	18910	0.0	20.058037	12-13
CAGAGTA	28685	0.0	19.49371	10-11
AGAGTAC	27020	0.0	18.619936	10-11
GTACTTT	20735	0.0	18.354208	14-15
>>END_MODULE