FastQCFastQC Report
Fri 27 May 2016
SRR2138779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7081409
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124750.050.6669351
CTTATAC71850.041.294791
GTATCAA217700.035.4044841
TATACAC95850.033.3664443
TTATACA96850.031.0692482
ATCAACG254500.029.7566723
TCAACGC263250.028.7977074
AACGCAG269950.028.0413326
CAACGCA270250.028.029315
TATCAAC274800.027.787892
ACGCAGA274950.027.5772327
ACACATC109850.026.806816
ACATCTC122800.024.1167038
CGCAGAG315500.024.0195228
TACACAT130850.023.7917775
ATACACA151800.022.4511624
CACATCT135850.021.676327
GCAGAGT360200.021.0620789
GAGTACT219000.020.78317812-13
GTACATG238650.020.251341
Produced by FastQC (version 0.11.5)