##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138779_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7081409
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89146199576948	33.0	33.0	33.0	33.0	33.0
2	32.94738490602647	33.0	33.0	33.0	33.0	33.0
3	33.61535592704785	33.0	33.0	33.0	33.0	37.0
4	36.78036334294489	37.0	37.0	37.0	37.0	37.0
5	36.7932929731922	37.0	37.0	37.0	37.0	37.0
6	36.796312428783594	37.0	37.0	37.0	37.0	37.0
7	36.78853939943308	37.0	37.0	37.0	37.0	37.0
8	36.77716002563896	37.0	37.0	37.0	37.0	37.0
9	36.78211158259606	37.0	37.0	37.0	37.0	37.0
10-11	36.774694697058166	37.0	37.0	37.0	37.0	37.0
12-13	37.02703967529626	37.0	37.0	37.0	37.0	38.5
14-15	39.095695150499004	40.0	40.0	40.0	37.0	40.0
16-17	39.053382737813905	40.0	40.0	40.0	37.0	40.0
18-19	39.03681873197834	40.0	40.0	40.0	37.0	40.0
20-21	39.094747083980494	40.0	40.0	40.0	37.0	40.0
22-23	39.12519733346015	40.0	40.0	40.0	37.0	40.0
24-25	39.107406231968056	40.0	40.0	40.0	37.0	40.0
26-27	38.84100170571656	40.0	40.0	40.0	37.0	40.0
28-29	38.866316352925	40.0	40.0	40.0	37.0	40.0
30-31	38.867972525487346	40.0	40.0	40.0	37.0	40.0
32-33	38.849441543944835	40.0	40.0	40.0	37.0	40.0
34-35	38.8217592189869	40.0	37.0	40.0	37.0	40.0
36-37	38.79299626214474	40.0	37.0	40.0	37.0	40.0
38-39	38.73692581624593	40.0	37.0	40.0	37.0	40.0
40-41	38.6999890365106	40.0	37.0	40.0	37.0	40.0
42-43	38.64304634823086	40.0	37.0	40.0	37.0	40.0
44-45	38.56902985320502	40.0	37.0	40.0	37.0	40.0
46-47	38.493728377806775	40.0	37.0	40.0	37.0	40.0
48-49	38.44305377602831	40.0	37.0	40.0	37.0	40.0
50-51	38.379062782599334	40.0	37.0	40.0	37.0	40.0
52-53	38.31614577171426	40.0	37.0	40.0	37.0	40.0
54-55	38.2256940782853	40.0	37.0	40.0	37.0	40.0
56-57	38.10846153725451	40.0	37.0	40.0	37.0	40.0
58-59	37.97462022917492	40.0	37.0	40.0	37.0	40.0
60-61	37.807936571898274	40.0	37.0	40.0	33.0	40.0
62-63	37.61750502092464	38.5	37.0	40.0	33.0	40.0
64-65	37.40651248380762	37.0	37.0	40.0	33.0	40.0
66-67	37.17559134214889	37.0	37.0	40.0	33.0	40.0
68-69	36.92486031702186	37.0	37.0	40.0	33.0	40.0
70-71	36.678006145255935	37.0	37.0	40.0	33.0	40.0
72-73	36.43075355951456	37.0	37.0	37.0	33.0	40.0
74-75	35.955430908716735	37.0	35.0	37.0	33.0	40.0
76-77	34.13704583630002	37.0	33.0	37.0	27.0	37.0
78-79	31.85402758303009	37.0	33.0	37.0	10.5	37.0
80-81	34.668186504751134	37.0	33.0	37.0	30.0	37.0
82-83	35.56669527897242	37.0	35.0	37.0	33.0	37.0
84-85	35.665879615784476	37.0	37.0	37.0	33.0	37.0
86-87	35.62908553604428	37.0	37.0	37.0	33.0	37.0
88-89	35.55668727109692	37.0	37.0	37.0	33.0	37.0
90-91	35.4878588873733	37.0	33.0	37.0	33.0	37.0
92-93	35.41868444823946	37.0	33.0	37.0	33.0	37.0
94-95	35.375703075665434	37.0	33.0	37.0	33.0	37.0
96-97	35.350363001872395	37.0	33.0	37.0	33.0	37.0
98-99	35.308676838938865	37.0	33.0	37.0	33.0	37.0
100	35.245371535018045	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	0.0
15	0.0
16	0.0
17	1.0
18	6.0
19	1.0
20	28.0
21	133.0
22	442.0
23	1231.0
24	3006.0
25	6219.0
26	11267.0
27	19024.0
28	28952.0
29	40408.0
30	56252.0
31	75772.0
32	101331.0
33	139997.0
34	204269.0
35	330839.0
36	724202.0
37	2222368.0
38	2945323.0
39	170336.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.707867681853855	0.0	24.442796305300078	29.849336012846074
2	15.086042794039564	23.7794999830541	38.43672684343121	22.69773037947513
3	16.738635575683723	24.127211336935524	32.96715028963855	26.167002797742207
4	13.15372729147422	23.432091232411576	36.46232877679754	26.95185269931666
5	17.50465538828092	35.612954160755486	32.186983838017206	14.695406612946392
6	28.244679589143857	36.98281471707322	19.870808178260592	14.901697515522336
7	26.29457305665766	32.66353245004383	22.074889626469766	18.967004866828745
8	24.354111498730198	32.02140026390232	21.594773242835323	22.02971499453216
9	23.830924584489413	20.379690027745895	23.34377570110351	32.445609686661186
10-11	24.775854972919582	25.921072057910514	28.009646668455858	21.293426300714046
12-13	25.253660676851176	23.97356938428496	28.058144925677926	22.714625013185934
14-15	22.69583637945499	26.19657330906886	26.19460336212751	24.91298694934864
16-17	21.799877679710352	28.779138445470387	27.03976002515883	22.38122384966043
18-19	21.89896106834106	27.504166190654995	29.07464460815637	21.522228132847573
20-21	22.616216567359203	26.79268011533482	29.067234749857807	21.523868567448172
22-23	22.43641552634755	26.666995745769867	28.84515338158085	22.051435346301734
24-25	22.199667985306583	26.922350946595085	29.1952034473015	21.682777620796838
26-27	22.254354630177396	27.182306271414014	28.900545664938655	21.662793433469936
28-29	21.825317643009758	26.81974468552415	29.309864644212375	22.045073027253714
30-31	22.291699355827255	26.9933988852096	29.083554734140165	21.631347024822983
32-33	22.073756795236037	27.03610117065199	28.956870858119032	21.933271175992942
34-35	21.99431089415737	26.99979897781106	29.23863112784122	21.767259000190347
36-37	22.191826206444308	27.038642435801396	28.965976138746473	21.80355521900782
38-39	21.924412002871037	27.084201542500118	29.0779500052398	21.913436449389042
40-41	22.18712122680776	26.809358578658525	28.838852288111976	22.164667906421737
42-43	22.256582559272733	26.844311567855772	28.790101017885526	22.109004854985965
44-45	22.088132749826762	27.3341958916527	28.517415372823063	22.06025598569747
46-47	22.44597203579634	26.944107958408893	27.976983613010496	22.632936392784266
48-49	22.251596058200203	27.175691560287135	28.131344776667234	22.44136760484543
50-51	22.231479885935578	27.50925222864467	27.810036064597625	22.44923182082213
52-53	22.398636478255877	27.35906105577641	27.95699088415416	22.28531158181355
54-55	22.24750205883332	27.456492238861784	27.885553255947688	22.41045244635721
56-57	22.50747882835944	27.97959848342435	27.2137215903157	22.29920109790051
58-59	22.301128721859158	27.77278707246215	27.751978368942765	22.174105836735926
60-61	22.511585502907522	28.082733983955276	27.368970348949095	22.036710164188104
62-63	22.244216009116748	28.346638676536458	27.295265248891415	22.113880065455376
64-65	22.386083804495577	27.947158532844398	27.290322917843486	22.376434744816546
66-67	22.333676530942093	27.88454283932173	27.33197409302498	22.449806536711193
68-69	22.44530763775311	27.808187094490666	27.394174481349364	22.35233078640686
70-71	22.525053636222715	27.34517769111949	27.44668485527067	22.683083817387125
72-73	22.474528517755225	27.47349844801988	27.40665501350494	22.645318020719955
74-75	22.36968743064158	27.702474953938815	27.37053644966485	22.557301165754755
76-77	22.706442455467332	27.386170739538812	27.179779558707217	22.727607246286638
78-79	22.796827767650484	27.485000752553905	27.1233440785047	22.59482740129091
80-81	22.54814663124425	27.580064156328092	27.30542812547043	22.566361086957222
82-83	22.647709487951463	27.365753301549073	27.331335693381142	22.65520151711832
84-85	22.619642634289715	27.459877076428192	27.3992369127788	22.521243376503293
86-87	22.533306706628256	27.511899481676377	27.37331142910048	22.581482382594892
88-89	22.63650487183244	27.295570889985214	27.36831836558254	22.699605872599804
90-91	22.6637003096366	27.323246814382784	27.4347328682608	22.578320007719817
92-93	22.584760890466956	27.363293191969625	27.465030866246575	22.586915051316847
94-95	22.659703100074175	27.239488134201444	27.445659230056236	22.655149535668144
96-97	22.68333395012984	27.56521902123526	27.538813722488676	22.212633306146227
98-99	23.72799353583028	29.66031867744652	27.051464278108917	19.56022350861429
100	22.85713359285937	29.95785561637343	21.43129860202348	25.753712188743716
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1553.0
1	1383.5
2	1889.0
3	2799.0
4	4012.0
5	6548.5
6	8556.0
7	10142.0
8	11676.0
9	12195.0
10	11667.5
11	10997.0
12	11989.0
13	15080.5
14	22970.0
15	34652.0
16	44992.0
17	51449.0
18	52273.5
19	48680.0
20	43218.0
21	37177.0
22	33023.5
23	32603.5
24	35993.5
25	43314.5
26	53413.0
27	64830.0
28	77516.0
29	92151.0
30	107009.5
31	121509.5
32	136787.0
33	152063.0
34	168062.0
35	182506.5
36	197317.5
37	212283.0
38	224230.5
39	235396.5
40	245999.5
41	255234.5
42	262437.5
43	269957.0
44	278044.5
45	289455.5
46	302802.5
47	311844.0
48	311871.0
49	305923.5
50	299784.0
51	288205.0
52	272253.5
53	256267.0
54	241916.0
55	227891.0
56	211830.5
57	192438.5
58	171358.0
59	150545.5
60	127863.0
61	105769.5
62	85243.5
63	66389.5
64	50775.0
65	38284.5
66	28911.5
67	21985.0
68	16745.0
69	12257.0
70	8669.0
71	6230.0
72	4351.0
73	2962.5
74	1970.0
75	1339.0
76	875.5
77	550.0
78	349.0
79	220.5
80	156.5
81	122.0
82	88.0
83	55.5
84	34.0
85	21.5
86	16.5
87	12.5
88	8.5
89	4.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.106726359118644E-4
2	6.919526890764254E-4
3	0.004829547340084438
4	0.020744459188842223
5	0.01276582103928752
6	1.4121483450539292E-5
7	1.4121483450539292E-5
8	1.4121483450539292E-5
9	1.4121483450539292E-5
10-11	7.060741725269646E-6
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.943223895770288E-4
24-25	0.01320113923924568
26-27	0.05628524451861843
28-29	0.08425213311596655
30-31	0.07015909003709773
32-33	0.03159147462944931
34-35	0.0034083662512254817
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	4.5667880189098515E-5
54-55	6.058738937145338E-4
56-57	7.813351563455515E-4
58-59	1.5616285380744167E-5
60-61	1.5767681503555652E-5
62-63	2.3884169421241193E-5
64-65	3.216352191851549E-5
66-67	7.317063057717724E-5
68-69	1.9736777181654006E-4
70-71	0.001271004069123681
72-73	0.0162302917436858
74-75	0.06522101040287805
76-77	0.9259744648385667
78-79	3.9322059150788307
80-81	0.0010622084096432853
82-83	0.01663446157536088
84-85	0.0482703123285414
86-87	0.0354866854978373
88-89	0.007959521391621687
90-91	5.503669204530841E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	159.0
20-21	575.0
22-23	1149.0
24-25	2289.0
26-27	3625.0
28-29	7025.0
30-31	14457.0
32-33	19133.0
34-35	27517.0
36-37	38136.0
38-39	44183.0
40-41	45982.0
42-43	55960.0
44-45	67224.0
46-47	65652.0
48-49	56842.0
50-51	50297.0
52-53	48697.0
54-55	53970.0
56-57	59668.0
58-59	60723.0
60-61	61661.0
62-63	62441.0
64-65	65421.0
66-67	71578.0
68-69	63947.0
70-71	55900.0
72-73	53064.0
74-75	54302.0
76-77	56270.0
78-79	56728.0
80-81	55961.0
82-83	55253.0
84-85	56014.0
86-87	58684.0
88-89	63403.0
90-91	65818.0
92-93	68576.0
94-95	87411.0
96-97	322539.0
98-99	1222326.0
100-101	3700849.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.89937458591761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.2247263893006	77.50247569026459
2	6.970242313359884	11.974789108598271
3	1.61052213771989	4.150285331607408
4	0.5661777021228555	1.9453724206738094
5	0.24436343853166198	1.0495333270767042
6	0.12127802485739664	0.6250623891759445
7	0.0791913757974286	0.4761742757518243
8	0.041197143935633035	0.28310471190375247
9	0.0291728382905816	0.22553357076513622
>10	0.11027441336993106	1.5215259254669145
>50	0.002201377301476795	0.12900708339653946
>100	6.267574002441145E-4	0.10046354229470573
>500	2.6088012464444422E-5	0.01667262302434737
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.060741725269646E-5	0.0	0.0	0.0
3	0.0	7.060741725269646E-5	0.0	1.4121483450539292E-5	0.0
4	0.0	7.060741725269646E-5	0.0	1.4121483450539292E-5	0.0
5	0.0	8.472890070323576E-5	0.0	1.4121483450539292E-5	0.0
6	2.8242966901078583E-5	8.472890070323576E-5	0.0	1.4121483450539292E-5	1.4121483450539292E-5
7	2.8242966901078583E-5	8.472890070323576E-5	0.0	1.4121483450539292E-5	2.8242966901078583E-5
8	2.8242966901078583E-5	8.472890070323576E-5	0.0	5.6485933802157166E-5	2.8242966901078583E-5
9	5.6485933802157166E-5	1.2709335105485364E-4	0.0	8.472890070323576E-5	2.8242966901078583E-5
10-11	5.6485933802157166E-5	1.2709335105485364E-4	0.0	9.885038415377505E-5	2.8242966901078583E-5
12-13	5.6485933802157166E-5	1.2709335105485364E-4	0.0	1.0591112587904469E-4	2.8242966901078583E-5
14-15	8.472890070323576E-5	2.2594373520862867E-4	0.0	1.482755762306626E-4	5.6485933802157166E-5
16-17	1.4121483450539292E-4	2.6830818556024656E-4	0.0	1.835792848570108E-4	5.6485933802157166E-5
18-19	1.4827557623066256E-4	2.6830818556024656E-4	0.0	2.0476151003281974E-4	7.060741725269646E-5
20-21	1.553363179559322E-4	2.6830818556024656E-4	0.0	2.612474438349769E-4	7.060741725269646E-5
22-23	1.553363179559322E-4	2.9655115246132513E-4	0.0	3.3891560281294297E-4	7.76681589779661E-5
24-25	1.553363179559322E-4	3.8834079488983054E-4	0.0	4.024622783403699E-4	9.885038415377505E-5
26-27	1.553363179559322E-4	4.2364450351617877E-4	0.0	4.8719117904360556E-4	9.885038415377505E-5
28-29	1.553363179559322E-4	4.3776598696671805E-4	0.0	8.331675235818182E-4	9.885038415377505E-5
30-31	1.553363179559322E-4	5.013126624941449E-4	0.0	0.0016663350471636364	9.885038415377505E-5
32-33	1.553363179559322E-4	5.083734042194146E-4	0.0	0.0030078759749648694	9.885038415377505E-5
34-35	1.553363179559322E-4	5.57798596296302E-4	0.0	0.005076673300468876	1.1297186760431433E-4
36-37	1.553363179559322E-4	6.213452718237288E-4	0.0	0.008063367050257935	1.1297186760431433E-4
38-39	1.694578014064715E-4	6.495882387248075E-4	0.0	0.012215083184716488	1.1297186760431433E-4
40-41	1.694578014064715E-4	6.77831205625886E-4	0.0	0.0163526778357245	1.1297186760431433E-4
42-43	1.835792848570108E-4	7.554993646038521E-4	0.0	0.02037023987740293	1.1297186760431433E-4
44-45	1.835792848570108E-4	8.331675235818182E-4	0.0	0.025100936833333594	1.1297186760431433E-4
46-47	1.835792848570108E-4	8.331675235818182E-4	0.0	0.030657740571120806	1.1297186760431433E-4
48-49	1.9064002658228043E-4	8.331675235818182E-4	0.0	0.036087450957853165	1.2003260932958399E-4
50-51	2.0476151003281974E-4	9.885038415377505E-4	0.0	0.041587768761838215	1.4121483450539292E-4
52-53	2.1182225175808938E-4	0.0010026253249882898	0.0	0.046897446539240994	1.553363179559322E-4
54-55	2.1182225175808938E-4	0.0010026253249882898	0.0	0.05200236280661094	1.694578014064715E-4
56-57	2.1182225175808938E-4	0.0010238075501640986	0.0	0.057170825749508325	1.694578014064715E-4
58-59	2.1182225175808938E-4	0.001059111258790447	0.0	0.06272762948729554	1.694578014064715E-4
60-61	2.1182225175808938E-4	0.0011014757091420647	0.0	0.06853861992719246	1.694578014064715E-4
62-63	2.2594373520862867E-4	0.0011297186760431434	0.0	0.07391890512184793	2.1182225175808938E-4
64-65	2.2594373520862867E-4	0.0011862046098453005	0.0	0.0799487785552282	2.1182225175808938E-4
66-67	2.2594373520862867E-4	0.0011862046098453005	0.0	0.0851384237233014	2.1888299348335902E-4
68-69	2.3300447693389833E-4	0.0011862046098453005	0.0	0.09005976070581434	2.2594373520862867E-4
70-71	2.4006521865916797E-4	0.0011862046098453005	0.0	0.09393610791298737	2.4006521865916797E-4
72-73	2.8242966901078585E-4	0.0012073868350211095	0.0	0.09693692314622698	2.4006521865916797E-4
74-75	2.8242966901078585E-4	0.0012426905436474576	0.0	0.09964118722700524	2.471259603844376E-4
76-77	2.8242966901078585E-4	0.001292115735724345	0.0	0.10180177419493776	2.541867021097073E-4
78-79	2.8242966901078585E-4	0.0013274194443506934	0.0	0.10317155808964007	2.541867021097073E-4
80-81	2.8242966901078585E-4	0.0013274194443506934	0.0	0.10366581001040895	2.6124744383497695E-4
82-83	2.8242966901078585E-4	0.0013274194443506934	0.0	0.10402590783839769	2.6830818556024656E-4
84-85	2.8242966901078585E-4	0.0013697838947023112	0.0	0.10417418341462836	2.6830818556024656E-4
86-87	2.8242966901078585E-4	0.0013839053781528506	0.0	0.10429421602395794	2.753689272855162E-4
88	2.8242966901078585E-4	0.001454512795405547	0.0	0.10430127676568321	2.8242966901078585E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12475	0.0	50.666935	1
CTTATAC	7185	0.0	41.29479	1
GTATCAA	21770	0.0	35.404484	1
TATACAC	9585	0.0	33.366444	3
TTATACA	9685	0.0	31.069248	2
ATCAACG	25450	0.0	29.756672	3
TCAACGC	26325	0.0	28.797707	4
AACGCAG	26995	0.0	28.041332	6
CAACGCA	27025	0.0	28.02931	5
TATCAAC	27480	0.0	27.78789	2
ACGCAGA	27495	0.0	27.577232	7
ACACATC	10985	0.0	26.80681	6
ACATCTC	12280	0.0	24.116703	8
CGCAGAG	31550	0.0	24.019522	8
TACACAT	13085	0.0	23.791777	5
ATACACA	15180	0.0	22.451162	4
CACATCT	13585	0.0	21.67632	7
GCAGAGT	36020	0.0	21.062078	9
GAGTACT	21900	0.0	20.783178	12-13
GTACATG	23865	0.0	20.25134	1
>>END_MODULE