FastQCFastQC Report
Fri 27 May 2016
SRR2138778_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138778_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6492118
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92850.052.0469131
GTATCAA175450.039.179271
CTTATAC53450.038.1143571
TCAACGC218250.030.9909734
ATCAACG219200.030.8571363
TATCAAC225200.030.2042732
CAACGCA223750.030.2009455
AACGCAG229050.029.5338616
TATACAC83750.027.814363
TTATACA78650.027.0331212
ACGCAGA259750.026.0269417
CGCAGAG263850.025.6545938
GCAGAGT304600.022.2363669
GTGGTAT43650.022.219921
ACATCTC91700.021.927958
ACACATC93550.021.765826
TACACAT109100.020.9953545
TGGTATC42800.020.9789282
GAGTACT187300.020.30738812-13
GTACATG203200.019.967931
Produced by FastQC (version 0.11.5)