##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138778_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6492118
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83746752600615	33.0	33.0	33.0	33.0	33.0
2	32.83422898351509	33.0	33.0	33.0	33.0	33.0
3	32.83256142294395	33.0	33.0	33.0	33.0	33.0
4	36.783324332675406	37.0	37.0	37.0	37.0	37.0
5	36.794358019986696	37.0	37.0	37.0	37.0	37.0
6	36.804242313525414	37.0	37.0	37.0	37.0	37.0
7	36.7907561137983	37.0	37.0	37.0	37.0	37.0
8	36.7857067601051	37.0	37.0	37.0	37.0	37.0
9	36.785671332529695	37.0	37.0	37.0	37.0	37.0
10-11	36.76551204090868	37.0	37.0	37.0	37.0	37.0
12-13	36.75261001109345	37.0	37.0	37.0	37.0	37.0
14-15	39.11420502523214	40.0	40.0	40.0	37.0	40.0
16-17	39.07443803393592	40.0	40.0	40.0	37.0	40.0
18-19	39.05500608584133	40.0	40.0	40.0	37.0	40.0
20-21	39.10169378879064	40.0	40.0	40.0	37.0	40.0
22-23	39.13297428677805	40.0	40.0	40.0	37.0	40.0
24-25	39.13794988672665	40.0	40.0	40.0	37.0	40.0
26-27	38.87350847795004	40.0	40.0	40.0	37.0	40.0
28-29	38.87583592237467	40.0	40.0	40.0	37.0	40.0
30-31	38.88849363684881	40.0	40.0	40.0	37.0	40.0
32-33	38.87981850530004	40.0	40.0	40.0	37.0	40.0
34-35	38.85973129209968	40.0	40.0	40.0	37.0	40.0
36-37	38.82192954837447	40.0	40.0	40.0	37.0	40.0
38-39	38.77769853977183	40.0	37.0	40.0	37.0	40.0
40-41	38.7329730606959	40.0	37.0	40.0	37.0	40.0
42-43	38.674734393437774	40.0	37.0	40.0	37.0	40.0
44-45	38.605926732908316	40.0	37.0	40.0	37.0	40.0
46-47	38.527042160262006	40.0	37.0	40.0	37.0	40.0
48-49	38.46945167185593	40.0	37.0	40.0	37.0	40.0
50-51	38.40913024027887	40.0	37.0	40.0	37.0	40.0
52-53	38.34178014746571	40.0	37.0	40.0	37.0	40.0
54-55	38.269405671588075	40.0	37.0	40.0	37.0	40.0
56-57	38.16255704124572	40.0	37.0	40.0	37.0	40.0
58-59	38.03121256588631	40.0	37.0	40.0	37.0	40.0
60-61	37.86617150058649	40.0	37.0	40.0	35.0	40.0
62-63	37.6806511971474	38.5	37.0	40.0	33.0	40.0
64-65	37.471535198355255	37.0	37.0	40.0	33.0	40.0
66-67	37.2465352511142	37.0	37.0	40.0	33.0	40.0
68-69	36.99423274789889	37.0	37.0	40.0	33.0	40.0
70-71	36.745818199162265	37.0	37.0	40.0	33.0	40.0
72-73	36.50556802021465	37.0	37.0	37.0	33.0	40.0
74-75	36.24704056859014	37.0	37.0	37.0	33.0	40.0
76-77	34.552606105861585	35.0	33.0	37.0	30.0	37.0
78-79	31.433463739299533	37.0	33.0	37.0	10.5	37.0
80-81	34.51777297509898	37.0	33.0	37.0	30.0	37.0
82-83	35.56136568416535	37.0	35.0	37.0	33.0	37.0
84-85	35.684787597655955	37.0	37.0	37.0	33.0	37.0
86-87	35.65167226810339	37.0	37.0	37.0	33.0	37.0
88-89	35.57469033903148	37.0	37.0	37.0	33.0	37.0
90-91	35.507428857291266	37.0	35.0	37.0	33.0	37.0
92-93	35.438091150418046	37.0	33.0	37.0	33.0	37.0
94-95	35.393447140862996	37.0	33.0	37.0	33.0	37.0
96-97	35.37244481941191	37.0	33.0	37.0	33.0	37.0
98-99	35.33651836321067	37.0	33.0	37.0	33.0	37.0
100	35.289021806308654	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	18.0
21	85.0
22	399.0
23	1130.0
24	2686.0
25	5558.0
26	10161.0
27	17088.0
28	25586.0
29	36606.0
30	51086.0
31	68771.0
32	92406.0
33	127897.0
34	186153.0
35	300993.0
36	662368.0
37	2054444.0
38	2717138.0
39	131540.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93190881619835	19.795727680858544	14.451678173440472	23.820685329502638
2	14.677290215612224	20.669926208981416	41.623504070628414	23.029279504777946
3	18.17529977193827	25.716986578461025	30.516418257137673	25.591295392463024
4	12.496171398109889	18.170159399256157	38.19346996126718	31.140199241366773
5	13.591447313689905	37.23653763792046	35.24068488043555	13.931330167954078
6	30.159972446588306	37.30335770237078	18.613093600578424	13.923576250462483
7	26.64657358353622	32.6564766690932	22.37428524866615	18.32266449870443
8	24.629866555105746	36.12505194760786	20.961017652482596	18.284063844803807
9	25.56752665308918	16.86508162667407	20.866549252493563	36.70084246774319
10-11	24.19133478473435	26.645179585460397	28.474228287286213	20.689257342519035
12-13	25.27719767262394	24.224852351728664	28.547455545324347	21.950494430323047
14-15	22.397844586312203	25.592079503176006	26.36720404650686	25.642871864004935
16-17	21.85771269098929	28.831307440807453	26.998662069913088	22.312317798290174
18-19	21.97411538114372	27.62580717109578	28.900167865094257	21.499909582666245
20-21	22.616951906389954	26.770782573709422	29.017503169865105	21.594762350035516
22-23	22.241465284616833	26.880927832431155	28.924779831894455	21.952827051057557
24-25	22.1775597119349	26.98270496871775	28.930612934207506	21.909122385139842
26-27	22.154465197924452	27.113935502683795	28.8942150782818	21.83738422110995
28-29	21.889516975825835	27.0474137515259	29.01980291156146	22.043266361086804
30-31	22.161600303303572	26.992766413764347	28.97988219177617	21.865751091155914
32-33	21.80473166511301	27.000690924040683	29.140853095032504	22.053724315813803
34-35	21.926545631523304	27.158026642542694	28.898797294616855	22.016630431317147
36-37	22.00132562876834	27.174883548006225	28.896409767100685	21.927381056124748
38-39	21.89255201112177	27.11633594357419	28.921140820813097	22.06997122449095
40-41	22.141393695364396	27.01884909337241	28.851073870530907	21.98868334073229
42-43	22.163205023494864	27.107176243267133	28.64738944312897	22.082229290109034
44-45	22.0989722584356	27.340545459375736	28.425629171229126	22.134853110959536
46-47	22.41003416057068	27.239277367446174	27.880759778154268	22.469928693828876
48-49	22.264608233948383	27.37074464163016	27.871793901373497	22.492853223047955
50-51	22.198961482006936	27.579582693164994	27.84187250667402	22.379583318154047
52-53	22.339071083994906	27.558318095449064	27.89209216972533	22.2105186508307
54-55	22.272063164638244	27.513438192659688	27.719474066453937	22.49502457624813
56-57	22.463350729820977	27.756181318912525	27.437348574644936	22.34311937662156
58-59	22.224265497143648	27.887425513502293	27.754301341336408	22.13400764801765
60-61	22.477386287239213	28.010224571180682	27.374576903070967	22.137812238509134
62-63	22.238486898254642	28.195875598131614	27.377468636577547	22.1881688670362
64-65	22.269752567319067	28.038072739834536	27.367272308545864	22.324902384300536
66-67	22.35567455803761	27.962203130633227	27.271800339270463	22.4103219720587
68-69	22.37999529928578	27.770578071177077	27.39949952266999	22.449927106867147
70-71	22.36309474089484	27.57299921946067	27.4746158377104	22.589290201934094
72-73	22.436850249231405	27.589567989223678	27.3918303774905	22.581751384054417
74-75	22.327095050419217	27.715501718925385	27.356241051315173	22.60116217934022
76-77	22.47131949829232	27.674093988920532	27.288706020715548	22.5658804920716
78-79	22.91821691250531	27.585810058855998	26.930621558601274	22.565351470037413
80-81	22.426902679359248	27.715660433373063	27.279040336383144	22.57839655088455
82-83	22.474361815249434	27.702060402343818	27.321268174961723	22.502309607445024
84-85	22.549464979188553	27.66324882815528	27.286324886648295	22.500961306007873
86-87	22.43147475303172	27.666898599908578	27.38748577982281	22.514140867236886
88-89	22.483096380055276	27.671287622646528	27.304633551513763	22.54098244578443
90-91	22.508389492782772	27.677098819277045	27.311442590027447	22.503069097912736
92-93	22.5389241567984	27.650781916820392	27.33254724585071	22.4777466805305
94-95	22.433625363851114	27.71817857898013	27.34709666182862	22.50109939534014
96-97	22.51800273295862	27.825631156632564	27.386545121459232	22.269820988949583
98-99	23.648157285290733	29.74007298848276	27.569543533051515	19.042226193175
100	22.471753820645688	30.733108525388126	21.49154341734284	25.30359423662335
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1936.0
1	1725.5
2	2044.0
3	2764.5
4	3744.5
5	5844.5
6	7593.5
7	8919.5
8	10067.0
9	10421.0
10	10038.0
11	9706.0
12	10823.0
13	13949.5
14	21114.5
15	30863.5
16	38826.5
17	43817.0
18	45169.5
19	42677.0
20	38202.0
21	33428.0
22	30179.0
23	30284.5
24	33666.5
25	40185.0
26	49174.5
27	59763.5
28	71710.5
29	85125.5
30	98546.5
31	112308.5
32	127134.5
33	141621.5
34	155438.0
35	168834.0
36	183053.0
37	196694.5
38	208236.0
39	218506.5
40	229386.5
41	238076.0
42	243394.5
43	249117.5
44	254487.0
45	259603.5
46	266019.5
47	272904.5
48	280918.0
49	280218.5
50	271874.5
51	263559.0
52	252129.5
53	237799.5
54	222330.0
55	207757.0
56	191557.5
57	173649.5
58	155067.5
59	135383.5
60	114337.0
61	93601.5
62	75116.0
63	58901.0
64	44720.5
65	33432.5
66	24874.0
67	18687.0
68	14300.5
69	10537.5
70	7602.5
71	5475.5
72	3860.0
73	2645.5
74	1813.5
75	1213.0
76	787.5
77	496.0
78	317.5
79	211.0
80	150.0
81	115.0
82	88.0
83	61.5
84	38.0
85	21.0
86	15.0
87	13.0
88	10.0
89	8.0
90	6.0
91	4.5
92	3.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	2.4645269848761224E-4
4	0.023074133895902692
5	0.01629668468749336
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008551172527532465
26-27	0.05286384485216734
28-29	0.08558309575029042
30-31	0.07601525677456644
32-33	0.036192894280548074
34-35	0.004429186461613692
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.7556525700890316E-4
56-57	7.6585253736624E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	1.798270225887017E-5
72-73	0.013298072577723426
74-75	0.02760317521602664
76-77	0.001061434734870217
78-79	4.973765489455475
80-81	0.0
82-83	0.012866665572304255
84-85	0.04977244692343643
86-87	0.03893375837228079
88-89	0.01008778028990308
90-91	3.9483434280958784E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	69.0
20-21	348.0
22-23	900.0
24-25	1808.0
26-27	2903.0
28-29	5521.0
30-31	12830.0
32-33	16620.0
34-35	23097.0
36-37	33455.0
38-39	40023.0
40-41	41861.0
42-43	49650.0
44-45	60215.0
46-47	59370.0
48-49	51759.0
50-51	45297.0
52-53	43699.0
54-55	48492.0
56-57	51689.0
58-59	52905.0
60-61	51865.0
62-63	52640.0
64-65	55698.0
66-67	60976.0
68-69	54931.0
70-71	49607.0
72-73	47124.0
74-75	48034.0
76-77	48938.0
78-79	48735.0
80-81	49469.0
82-83	48703.0
84-85	48840.0
86-87	50652.0
88-89	53871.0
90-91	56112.0
92-93	58161.0
94-95	69567.0
96-97	204946.0
98-99	391661.0
100-101	4299077.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.36040556504283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.80406440093816	81.11844362986206
2	6.085826967378364	10.754922780724538
3	1.2463905788098266	3.3039473110825424
4	0.39858991660199317	1.4087826674035484
5	0.17641884029336258	0.7794220138818018
6	0.0903967986507439	0.47924986743367487
7	0.05653325233641976	0.34967107730498675
8	0.03040840799203758	0.2149519410210984
9	0.021318872895532456	0.16953698297149622
>10	0.08775877612070776	1.2351781884210777
>50	0.0017835799685193777	0.09962566113607826
>100	4.8407138567714856E-4	0.07096930464912794
>500	2.5536628596837004E-5	0.015298574107955033
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.540329365547576E-5	0.0
2	0.0	1.540329365547576E-5	0.0	1.540329365547576E-5	0.0
3	0.0	1.540329365547576E-5	0.0	3.080658731095152E-5	0.0
4	0.0	4.620988096642729E-5	0.0	3.080658731095152E-5	0.0
5	0.0	4.620988096642729E-5	0.0	3.080658731095152E-5	0.0
6	0.0	4.620988096642729E-5	0.0	3.080658731095152E-5	0.0
7	0.0	4.620988096642729E-5	0.0	3.080658731095152E-5	0.0
8	0.0	6.161317462190305E-5	0.0	3.080658731095152E-5	0.0
9	0.0	7.701646827737881E-5	0.0	3.080658731095152E-5	0.0
10-11	0.0	7.701646827737881E-5	0.0	3.080658731095152E-5	0.0
12-13	0.0	7.701646827737881E-5	0.0	3.8508234138689405E-5	7.70164682773788E-6
14-15	0.0	1.0782305558833034E-4	0.0	4.620988096642729E-5	3.080658731095152E-5
16-17	0.0	1.0782305558833034E-4	0.0	5.391152779416516E-5	4.620988096642729E-5
18-19	0.0	1.0782305558833034E-4	0.0	6.161317462190305E-5	5.391152779416516E-5
20-21	0.0	1.1552470241606822E-4	0.0	6.161317462190305E-5	7.701646827737881E-5
22-23	7.70164682773788E-6	1.4633128972701975E-4	0.0	7.701646827737881E-5	7.701646827737881E-5
24-25	1.540329365547576E-5	1.5403293655475762E-4	0.0	1.1552470241606822E-4	7.701646827737881E-5
26-27	1.540329365547576E-5	1.5403293655475762E-4	0.0	1.5403293655475762E-4	7.701646827737881E-5
28-29	1.540329365547576E-5	1.5403293655475762E-4	0.0	3.5427575407594256E-4	7.701646827737881E-5
30-31	1.540329365547576E-5	1.7713787703797128E-4	0.0	9.78109147122711E-4	7.701646827737881E-5
32-33	1.540329365547576E-5	1.8483952386570915E-4	0.0	0.002202670992733034	7.701646827737881E-5
34-35	1.540329365547576E-5	2.0024281752118492E-4	0.0	0.003958646469457271	7.701646827737881E-5
36-37	1.540329365547576E-5	2.3875105165987431E-4	0.0	0.006315350398745063	7.701646827737881E-5
38-39	1.540329365547576E-5	2.464526984876122E-4	0.0	0.009881212879987701	7.701646827737881E-5
40-41	1.540329365547576E-5	3.2346916676499103E-4	0.0	0.013878367583583663	7.701646827737881E-5
42-43	1.540329365547576E-5	3.3887246042046677E-4	0.0	0.0173826168902044	7.701646827737881E-5
44-45	1.540329365547576E-5	3.850823413868941E-4	0.0	0.02137206994697262	7.701646827737881E-5
46-47	1.540329365547576E-5	4.158889286978456E-4	0.0	0.02613168768651463	7.701646827737881E-5
48-49	1.540329365547576E-5	4.235905755255835E-4	0.0	0.03115316141819973	7.701646827737881E-5
50-51	1.540329365547576E-5	5.006070438029623E-4	0.0	0.03561241493145996	7.701646827737881E-5
52-53	1.540329365547576E-5	5.083086906307002E-4	0.0	0.039909933861337704	9.241976193285458E-5
54-55	1.540329365547576E-5	5.083086906307002E-4	0.0	0.04493910923985053	1.0782305558833034E-4
56-57	1.540329365547576E-5	5.083086906307002E-4	0.0	0.050114615908090396	1.232263492438061E-4
58-59	1.540329365547576E-5	5.16010337458438E-4	0.0	0.05496665340956526	1.3862964289928186E-4
60-61	1.540329365547576E-5	5.314136311139138E-4	0.0	0.05978788432372917	1.5403293655475762E-4
62-63	1.540329365547576E-5	5.545185715971274E-4	0.0	0.0644704855949938	1.6943623021023339E-4
64-65	1.540329365547576E-5	6.777449208409335E-4	0.0	0.0689836506360482	1.6943623021023339E-4
66-67	1.540329365547576E-5	6.777449208409335E-4	0.0	0.07312713662937119	1.6943623021023339E-4
68-69	1.540329365547576E-5	6.931482144964093E-4	0.0	0.07764800331725333	1.6943623021023339E-4
70-71	1.540329365547576E-5	6.931482144964093E-4	0.0	0.08174527942960988	1.6943623021023339E-4
72-73	2.310494048321364E-5	6.931482144964093E-4	0.0	0.08481053486704955	1.6943623021023339E-4
74-75	3.080658731095152E-5	7.239548018073609E-4	0.0	0.08733667502654757	1.7713787703797128E-4
76-77	3.080658731095152E-5	7.547613891183124E-4	0.0	0.08967797566217989	1.8483952386570915E-4
78-79	3.080658731095152E-5	7.701646827737882E-4	0.0	0.09123370832138294	1.8483952386570915E-4
80-81	3.080658731095152E-5	7.701646827737882E-4	0.0	0.09176512195249686	1.8483952386570915E-4
82-83	3.080658731095152E-5	7.701646827737882E-4	0.0	0.0923427454645772	1.8483952386570915E-4
84-85	3.080658731095152E-5	7.701646827737882E-4	0.0	0.09262000475037577	1.8483952386570915E-4
86-87	3.080658731095152E-5	7.701646827737882E-4	0.0	0.09268161792499767	1.8483952386570915E-4
88	3.080658731095152E-5	8.163745637402155E-4	0.0	0.09268161792499767	1.8483952386570915E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9285	0.0	52.046913	1
GTATCAA	17545	0.0	39.17927	1
CTTATAC	5345	0.0	38.114357	1
TCAACGC	21825	0.0	30.990973	4
ATCAACG	21920	0.0	30.857136	3
TATCAAC	22520	0.0	30.204273	2
CAACGCA	22375	0.0	30.200945	5
AACGCAG	22905	0.0	29.533861	6
TATACAC	8375	0.0	27.81436	3
TTATACA	7865	0.0	27.033121	2
ACGCAGA	25975	0.0	26.026941	7
CGCAGAG	26385	0.0	25.654593	8
GCAGAGT	30460	0.0	22.236366	9
GTGGTAT	4365	0.0	22.21992	1
ACATCTC	9170	0.0	21.92795	8
ACACATC	9355	0.0	21.76582	6
TACACAT	10910	0.0	20.995354	5
TGGTATC	4280	0.0	20.978928	2
GAGTACT	18730	0.0	20.307388	12-13
GTACATG	20320	0.0	19.96793	1
>>END_MODULE