FastQCFastQC Report
Fri 27 May 2016
SRR2138777_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138777_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6350873
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96650.052.0935021
GTATCAA182300.038.2818071
CTTATAC50750.033.069541
ATCAACG213050.032.083563
TCAACGC221650.030.8562874
CAACGCA228400.029.9938585
TATCAAC229950.029.9823322
AACGCAG230600.029.7217776
ACGCAGA234450.029.287647
TATACAC72500.026.1149653
CGCAGAG267950.025.5945388
GTGGTAT43200.024.597891
TGGTATC43100.023.774432
TTATACA75150.023.174062
GCAGAGT309050.022.1362119
ACACATC79750.020.9832786
GAGTACT188200.020.4710512-13
GTACATG206100.020.2348211
TACACAT94600.019.9200975
TACATGG205950.019.7991142
Produced by FastQC (version 0.11.5)