##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138777_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6350873
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89580660800492	33.0	33.0	33.0	33.0	33.0
2	32.950717484037234	33.0	33.0	33.0	33.0	33.0
3	33.61440718465005	33.0	33.0	33.0	33.0	37.0
4	36.7835381057061	37.0	37.0	37.0	37.0	37.0
5	36.79604063882241	37.0	37.0	37.0	37.0	37.0
6	36.80007551717693	37.0	37.0	37.0	37.0	37.0
7	36.79114084000735	37.0	37.0	37.0	37.0	37.0
8	36.78014093495493	37.0	37.0	37.0	37.0	37.0
9	36.78553483906858	37.0	37.0	37.0	37.0	37.0
10-11	36.778319610548024	37.0	37.0	37.0	37.0	37.0
12-13	37.03033874240597	37.0	37.0	37.0	37.0	38.5
14-15	39.10851075119909	40.0	40.0	40.0	37.0	40.0
16-17	39.06768187617671	40.0	40.0	40.0	37.0	40.0
18-19	39.049914633783416	40.0	40.0	40.0	37.0	40.0
20-21	39.10969931433446	40.0	40.0	40.0	37.0	40.0
22-23	39.13908459858507	40.0	40.0	40.0	37.0	40.0
24-25	39.12358778338149	40.0	40.0	40.0	37.0	40.0
26-27	38.85984745568779	40.0	40.0	40.0	37.0	40.0
28-29	38.88747023213398	40.0	40.0	40.0	37.0	40.0
30-31	38.89600222810972	40.0	40.0	40.0	37.0	40.0
32-33	38.87597450416446	40.0	40.0	40.0	37.0	40.0
34-35	38.849063430796264	40.0	40.0	40.0	37.0	40.0
36-37	38.81901400689548	40.0	38.5	40.0	37.0	40.0
38-39	38.77187644463074	40.0	37.0	40.0	37.0	40.0
40-41	38.72737274574834	40.0	37.0	40.0	37.0	40.0
42-43	38.66614962604097	40.0	37.0	40.0	37.0	40.0
44-45	38.59334887916704	40.0	37.0	40.0	37.0	40.0
46-47	38.51546703861938	40.0	37.0	40.0	37.0	40.0
48-49	38.462354720478814	40.0	37.0	40.0	37.0	40.0
50-51	38.400720220583835	40.0	37.0	40.0	37.0	40.0
52-53	38.33595873698533	40.0	37.0	40.0	37.0	40.0
54-55	38.24678273790031	40.0	37.0	40.0	37.0	40.0
56-57	38.13680000317892	40.0	37.0	40.0	37.0	40.0
58-59	38.00403741777414	40.0	37.0	40.0	37.0	40.0
60-61	37.83915546549963	40.0	37.0	40.0	33.0	40.0
62-63	37.64890044936911	38.5	37.0	40.0	33.0	40.0
64-65	37.43495210489776	37.0	37.0	40.0	33.0	40.0
66-67	37.20154338065014	37.0	37.0	40.0	33.0	40.0
68-69	36.950326098646975	37.0	37.0	40.0	33.0	40.0
70-71	36.7026062572849	37.0	37.0	40.0	33.0	40.0
72-73	36.456228217242156	37.0	37.0	37.0	33.0	40.0
74-75	35.979900845729006	37.0	37.0	37.0	33.0	40.0
76-77	34.15881686064132	37.0	33.0	37.0	27.0	37.0
78-79	31.85428740954585	37.0	33.0	37.0	10.5	37.0
80-81	34.67987014892411	37.0	33.0	37.0	30.0	37.0
82-83	35.58318210402511	37.0	35.0	37.0	33.0	37.0
84-85	35.68305834356751	37.0	37.0	37.0	33.0	37.0
86-87	35.6464063106486	37.0	37.0	37.0	33.0	37.0
88-89	35.57444867706988	37.0	37.0	37.0	33.0	37.0
90-91	35.50606619445552	37.0	35.0	37.0	33.0	37.0
92-93	35.43971779007707	37.0	33.0	37.0	33.0	37.0
94-95	35.39655245935077	37.0	33.0	37.0	33.0	37.0
96-97	35.37224475975225	37.0	33.0	37.0	33.0	37.0
98-99	35.326842091128384	37.0	33.0	37.0	33.0	37.0
100	35.26361562682829	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	0.0
18	2.0
19	6.0
20	29.0
21	92.0
22	356.0
23	1033.0
24	2556.0
25	5513.0
26	9924.0
27	16457.0
28	24824.0
29	35507.0
30	49309.0
31	66992.0
32	89877.0
33	124255.0
34	180809.0
35	293300.0
36	643387.0
37	1987076.0
38	2665861.0
39	153706.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.9348714669532	0.0	24.307239164730053	29.757889368316746
2	15.139294369508494	23.660179274176876	38.310750199462085	22.889776156852545
3	16.759750673014057	24.14432419562695	32.85417167936691	26.241753451992082
4	13.190133323169858	23.113507227459525	36.63525543764314	27.061104011727476
5	17.640676210855734	35.739748143771344	32.03658220855147	14.582993436821454
6	28.381231997553723	36.86894699358655	19.972561252602596	14.777259756257132
7	26.464235704288214	32.64535442607654	22.00755707128768	18.882852798347567
8	24.445442382488203	31.925705332164572	21.733248326647374	21.89560395869985
9	23.949416088150404	20.276409243264666	23.357828128510835	32.416346540074095
10-11	24.78417534093344	25.894928146098966	28.13893460001483	21.181961912952755
12-13	25.216202559868538	23.98741873754994	28.146177698719534	22.650201003861987
14-15	22.68780213365942	26.23190544040166	26.282866938135907	24.797425487803014
16-17	21.80795186928282	28.69878567059655	27.163760123315363	22.32950233680527
18-19	21.87322194650324	27.50863050978602	29.13623415876611	21.481913384944633
20-21	22.50969736535881	26.765515705842542	29.13246961670646	21.59231731209219
22-23	22.42513522123654	26.599304992685997	28.95089548292489	22.024664303152573
24-25	22.17002346384292	26.848480599993856	29.143471278255657	21.838024657907567
26-27	22.095351454037747	27.120392394112514	28.908702155290484	21.875553996559262
28-29	21.914802753528758	26.86629773805785	29.231931535585453	21.986967972827944
30-31	22.101054963146307	26.77609236734728	29.005945891682742	22.116906777823665
32-33	22.018249896343374	27.062703554710126	29.09477697208709	21.824269576859407
34-35	22.238261562903507	27.013521045188494	28.878112250261317	21.870105141646683
36-37	22.027778620944762	27.052132337434948	28.889091780093874	22.03099726152642
38-39	21.96107655133989	27.17797465835181	28.964874449508272	21.896074340800027
40-41	22.230898128890754	26.86591257234021	28.740924364639003	22.162264934130032
42-43	22.218678546568647	26.951959260648884	28.69432178933175	22.13504040345072
44-45	22.174781134578687	27.354492371444717	28.354516940060766	22.116209553915827
46-47	22.449490525860284	27.04350830911224	27.9380552096975	22.568945955329973
48-49	22.288845079664288	27.298017607078563	28.0248908375321	22.388246475725047
50-51	22.298005509923804	27.613691623700515	27.71343211830766	22.37487074806802
52-53	22.46252629638764	27.38848945444168	27.776217306658125	22.372766942512552
54-55	22.335251200378853	27.44309801485941	27.78266614583551	22.43898463892623
56-57	22.49680071712861	27.85233607298353	27.344361359226426	22.306501850661427
58-59	22.40050422553056	27.655081025507688	27.676241048376156	22.26817370058559
60-61	22.496650608704023	27.83654356672665	27.483098277484718	22.18370754708461
62-63	22.308686650622878	28.136997451868627	27.346439694224234	22.207876203284258
64-65	22.455929982611725	27.780572897949344	27.304674299056032	22.4588228203829
66-67	22.373840149015052	27.77623102419709	27.370557694404603	22.479371132383257
68-69	22.471757728127905	27.73358924080318	27.39146277951751	22.403190251551404
70-71	22.511273228529145	27.32307379115751	27.457670576461695	22.70798240385165
72-73	22.452276891087457	27.41165928578474	27.480935740167695	22.65512808296011
74-75	22.393190279703692	27.67613325795313	27.37715323464527	22.553523227697912
76-77	22.72075374637507	27.398350511725035	27.172940489864516	22.707955252035376
78-79	22.789141749011996	27.442128308807014	27.12519936045603	22.643530581724963
80-81	22.52450644588296	27.582568998845797	27.27910742148387	22.61381713378737
82-83	22.626071702459214	27.376878607313575	27.347200243658587	22.649849446568624
84-85	22.630525773518304	27.385475781199442	27.407270154435164	22.576728290847093
86-87	22.51328198694492	27.470811114745302	27.387556902049365	22.628349996260415
88-89	22.652557195303423	27.27146345196899	27.350589581508792	22.725389771218786
90-91	22.667636011787312	27.30502626229309	27.41268125206785	22.61465647385175
92-93	22.583370058722252	27.40294014145304	27.412241394336945	22.60144840548776
94-95	22.633251264739677	27.266230760780548	27.39408700034518	22.706430974134598
96-97	22.703687028738166	27.54748949016167	27.521297343209895	22.227526137890273
98-99	23.80087140490782	29.590669288981537	27.033841717554292	19.57461758855635
100	22.875418396477013	29.88831406164748	21.40716872052699	25.829098821348513
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1348.0
1	1228.0
2	1669.5
3	2425.5
4	3431.5
5	5555.5
6	7345.5
7	8871.0
8	10353.0
9	10923.0
10	10477.0
11	9705.5
12	10437.0
13	13246.5
14	20143.0
15	30171.0
16	39089.5
17	44810.0
18	45804.5
19	42433.5
20	37228.5
21	32170.5
22	28849.5
23	28993.0
24	32597.0
25	39202.5
26	48254.0
27	58494.5
28	69550.0
29	82443.5
30	96025.0
31	109532.5
32	123416.5
33	137090.5
34	150443.5
35	163688.5
36	177670.5
37	190862.5
38	202068.0
39	211687.5
40	221045.5
41	230098.5
42	236142.0
43	241340.5
44	247036.0
45	252494.5
46	258173.5
47	263818.5
48	271501.0
49	271831.0
50	265178.5
51	258949.5
52	248957.5
53	235654.0
54	221884.0
55	208100.5
56	192388.5
57	175013.0
58	156276.0
59	136429.0
60	115969.5
61	96249.0
62	77562.5
63	60625.5
64	46348.5
65	34852.0
66	26169.0
67	19931.5
68	15276.5
69	10993.5
70	7784.5
71	5727.5
72	4079.5
73	2814.0
74	1915.5
75	1266.5
76	828.0
77	523.0
78	351.5
79	240.5
80	150.5
81	103.0
82	79.5
83	54.5
84	34.5
85	28.0
86	25.0
87	19.0
88	13.0
89	9.0
90	4.5
91	2.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.5193386798948744E-4
2	6.613264034724045E-4
3	0.005306357094528579
4	0.02108371557737023
5	0.01418702594115801
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	2.3618800124014444E-5
18-19	3.936466687335741E-5
20-21	7.873149081699511E-5
22-23	5.19670144887186E-4
24-25	0.013073141074155425
26-27	0.05551760019008495
28-29	0.08412586939064634
30-31	0.06992967805275997
32-33	0.03198089404594538
34-35	0.003577980457721282
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	2.5434777853498055E-5
54-55	4.954306347141242E-4
56-57	6.718513492713126E-4
58-59	0.0
60-61	8.76861313510188E-6
62-63	0.0
64-65	0.0
66-67	5.4113798612792774E-5
68-69	1.91401602031409E-4
70-71	0.0015737222617646781
72-73	0.017550545571245187
74-75	0.06673816760771262
76-77	0.9255388567559315
78-79	3.944093999630659
80-81	0.0010316468767228263
82-83	0.016618289951678453
84-85	0.048168316218508
86-87	0.03501965846946518
88-89	0.008049479609172672
90-91	2.031034202615972E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	101.0
20-21	418.0
22-23	885.0
24-25	2143.0
26-27	3175.0
28-29	6247.0
30-31	13120.0
32-33	16916.0
34-35	24146.0
36-37	33311.0
38-39	39283.0
40-41	41490.0
42-43	49936.0
44-45	59518.0
46-47	57788.0
48-49	50216.0
50-51	44265.0
52-53	42899.0
54-55	47140.0
56-57	50994.0
58-59	51603.0
60-61	51055.0
62-63	51124.0
64-65	54009.0
66-67	59057.0
68-69	54538.0
70-71	49165.0
72-73	47678.0
74-75	48824.0
76-77	50407.0
78-79	50873.0
80-81	50254.0
82-83	49624.0
84-85	50011.0
86-87	52879.0
88-89	57308.0
90-91	58756.0
92-93	60948.0
94-95	78948.0
96-97	292200.0
98-99	1100915.0
100-101	3346706.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.13328695774445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.99637738823697	79.2881346308446
2	6.540486452002534	11.397881657311533
3	1.4211901709570463	3.7149891296257858
4	0.48859164258741306	1.702903831948991
5	0.2094751678627561	0.9126129955953607
6	0.11540973278919091	0.6033617618902307
7	0.0625372822671034	0.38143552729358887
8	0.04113764969252556	0.28675669083407984
9	0.03073762187716771	0.2410443024679708
>10	0.0918260241284058	1.2755712720803196
>50	0.001700566540056147	0.09628992412919078
>100	5.030914451638442E-4	0.08389216954589891
>500	2.720961368472551E-5	0.015126106432539562
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5745866749342965E-5
2	0.0	1.5745866749342965E-5	0.0	0.0	1.5745866749342965E-5
3	0.0	1.5745866749342965E-5	0.0	0.0	1.5745866749342965E-5
4	0.0	1.5745866749342965E-5	0.0	0.0	1.5745866749342965E-5
5	0.0	1.5745866749342965E-5	0.0	0.0	1.5745866749342965E-5
6	0.0	1.5745866749342965E-5	0.0	0.0	1.5745866749342965E-5
7	0.0	1.5745866749342965E-5	0.0	1.5745866749342965E-5	1.5745866749342965E-5
8	0.0	6.298346699737186E-5	0.0	1.5745866749342965E-5	1.5745866749342965E-5
9	1.5745866749342965E-5	1.1022106724540075E-4	0.0	3.149173349868593E-5	1.5745866749342965E-5
10-11	1.5745866749342965E-5	1.1022106724540075E-4	0.0	4.7237600248028896E-5	1.5745866749342965E-5
12-13	1.5745866749342965E-5	1.1022106724540075E-4	0.0	4.7237600248028896E-5	1.5745866749342965E-5
14-15	1.5745866749342965E-5	2.2044213449080153E-4	0.0	5.511053362270038E-5	4.7237600248028896E-5
16-17	1.5745866749342965E-5	2.5193386798948744E-4	0.0	6.298346699737186E-5	6.298346699737186E-5
18-19	1.5745866749342965E-5	2.5193386798948744E-4	0.0	7.872933374671482E-5	7.872933374671482E-5
20-21	1.5745866749342965E-5	2.5193386798948744E-4	0.0	1.1022106724540076E-4	7.872933374671482E-5
22-23	1.5745866749342965E-5	2.8342560148817336E-4	0.0	1.5745866749342964E-4	9.447520049605778E-5
24-25	2.3618800124014448E-5	3.3066320173620226E-4	0.0	1.8895040099211558E-4	1.1022106724540075E-4
26-27	3.149173349868593E-5	3.7790080198423116E-4	0.0	2.676797347388304E-4	1.2596693399474372E-4
28-29	3.149173349868593E-5	3.936466687335741E-4	0.0	4.56630135730946E-4	1.2596693399474372E-4
30-31	3.149173349868593E-5	4.2513840223226E-4	0.0	0.0012990340068207945	1.2596693399474372E-4
32-33	4.7237600248028896E-5	4.2513840223226E-4	0.0	0.002747653747760347	1.4171280074408668E-4
34-35	6.298346699737186E-5	4.8812186922963185E-4	0.0	0.004810362291924275	1.5745866749342964E-4
36-37	7.872933374671482E-5	5.353594694776607E-4	0.0	0.007802076974299439	1.5745866749342964E-4
38-39	7.872933374671482E-5	5.511053362270038E-4	0.0	0.011785781261883208	1.7320453424277262E-4
40-41	9.447520049605779E-5	6.140888032243756E-4	0.0	0.016084402884453838	2.1256920111613E-4
42-43	9.447520049605779E-5	7.243098704697764E-4	0.0	0.02031216810665242	2.28315067865473E-4
44-45	9.447520049605779E-5	8.345309377151771E-4	0.0	0.025028055198080643	2.3618800124014446E-4
46-47	1.0234813387072927E-4	8.345309377151771E-4	0.0	0.030310793492485208	2.8342560148817336E-4
48-49	1.1022106724540075E-4	8.345309377151771E-4	0.0	0.03521563098490554	3.3066320173620226E-4
50-51	1.1022106724540075E-4	9.368790715859064E-4	0.0	0.04058497154643149	3.3066320173620226E-4
52-53	1.1022106724540075E-4	9.604978717099209E-4	0.0	0.04611177077545087	3.542820018602167E-4
54-55	1.1022106724540075E-4	9.683708050845924E-4	0.0	0.051835393338837035	3.7790080198423116E-4
56-57	1.1022106724540075E-4	9.762437384592638E-4	0.0	0.05753539710209919	3.7790080198423116E-4
58-59	1.1022106724540075E-4	9.762437384592638E-4	0.0	0.0635503182003482	3.9364666873357415E-4
60-61	1.1022106724540075E-4	0.001015608405332621	0.0	0.06937628889760511	4.093925354829171E-4
62-63	1.1022106724540075E-4	0.0010549730722059787	0.0	0.07471413772563237	4.881218692296319E-4
64-65	1.1022106724540075E-4	0.0011809400062007223	0.0	0.07981579855241949	4.881218692296319E-4
66-67	1.1022106724540075E-4	0.0011809400062007223	0.0	0.08520875791406946	4.881218692296319E-4
68-69	1.1022106724540075E-4	0.0011888129395753939	0.0	0.09011359540648978	4.881218692296319E-4
70-71	1.2596693399474372E-4	0.0011966858729500654	0.0	0.09398707862682815	5.038677359789749E-4
72-73	1.2596693399474372E-4	0.0012045588063247367	0.0	0.0972858377108155	5.511053362270038E-4
74-75	1.2596693399474372E-4	0.0012517964065727656	0.0	0.10000987265845185	5.511053362270038E-4
76-77	1.2596693399474372E-4	0.0013147798735701375	0.0	0.10226153160360787	5.747241363510181E-4
78-79	1.2596693399474372E-4	0.001322652806944809	0.0	0.10395421227916224	5.825970697256897E-4
80-81	1.2596693399474372E-4	0.001322652806944809	0.0	0.10455255521563728	5.983429364750327E-4
82-83	1.2596693399474372E-4	0.001322652806944809	0.0	0.10509578761848962	6.140888032243756E-4
84-85	1.2596693399474372E-4	0.0013383986736941519	0.0	0.10530048388623107	6.140888032243756E-4
86-87	1.2596693399474372E-4	0.0013383986736941519	0.0	0.10533984855310444	6.140888032243756E-4
88	1.2596693399474372E-4	0.0014958573411875816	0.0	0.10533984855310444	6.140888032243756E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9665	0.0	52.093502	1
GTATCAA	18230	0.0	38.281807	1
CTTATAC	5075	0.0	33.06954	1
ATCAACG	21305	0.0	32.08356	3
TCAACGC	22165	0.0	30.856287	4
CAACGCA	22840	0.0	29.993858	5
TATCAAC	22995	0.0	29.982332	2
AACGCAG	23060	0.0	29.721777	6
ACGCAGA	23445	0.0	29.28764	7
TATACAC	7250	0.0	26.114965	3
CGCAGAG	26795	0.0	25.594538	8
GTGGTAT	4320	0.0	24.59789	1
TGGTATC	4310	0.0	23.77443	2
TTATACA	7515	0.0	23.17406	2
GCAGAGT	30905	0.0	22.136211	9
ACACATC	7975	0.0	20.983278	6
GAGTACT	18820	0.0	20.47105	12-13
GTACATG	20610	0.0	20.234821	1
TACACAT	9460	0.0	19.920097	5
TACATGG	20595	0.0	19.799114	2
>>END_MODULE