FastQCFastQC Report
Fri 27 May 2016
SRR2138776_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138776_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5439380
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80550.053.6830141
GTATCAA152300.039.1060261
TCAACGC186650.031.4526794
ATCAACG187600.031.3388213
TATCAAC192250.030.7581022
CAACGCA192650.030.5338275
AACGCAG196800.029.9332356
CTTATAC40450.027.5942881
ACGCAGA223450.026.3441567
CGCAGAG226000.026.1222978
GTGGTAT36250.024.4450951
TGGTATC36100.023.1305162
TATACAC60100.022.6835983
GCAGAGT263500.022.3562099
CTCGTAT52250.021.6846269
TCTCGTA53400.021.1378698
GTACATG184100.021.0351921
AATCTCG53950.020.8434246
GAGTACT157900.020.74439412-13
TACATGG184450.020.5333392
Produced by FastQC (version 0.11.5)