FastQCFastQC Report
Fri 27 May 2016
SRR2138775_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138775_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6069427
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83700.053.132831
GTATCAA156600.039.06511
CTTATAC50800.038.8522341
TCAACGC193450.031.0050564
ATCAACG195550.030.6505013
TATCAAC197900.030.5024052
CAACGCA199300.030.0897675
TATACAC73200.029.7060153
AACGCAG204350.029.3879786
TTATACA72350.028.4607452
ACGCAGA237350.025.3567
CGCAGAG240950.024.9948888
GTGGTAT38400.023.2515961
ACACATC90850.022.1439466
TACACAT97700.022.1215215
GTACATG208500.021.8213331
GCAGAGT279150.021.5285849
TGGTATC38350.021.3881682
ACATCTC94400.021.356458
TACATGG208700.021.2682042
Produced by FastQC (version 0.11.5)