FastQCFastQC Report
Fri 27 May 2016
SRR2138774_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138774_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5424507
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71600.051.675911
GTATCAA132650.038.537111
CTTATAC60700.032.0191231
TCAACGC163600.030.6484414
ATCAACG165750.030.2511713
CAACGCA168200.029.855325
TATCAAC170100.029.7025722
AACGCAG172900.029.0437536
TATACAC81450.026.7338073
ACGCAGA196550.025.6139457
CGCAGAG199450.025.2202018
TTATACA83150.024.3459432
GTGGTAT34700.022.3061081
TGGTATC32850.021.879292
GCAGAGT231550.021.613729
GTACATG156750.020.5385721
TACATGG156250.020.2504582
ACACATC97550.020.0942366
ACATCTC99550.019.946818
GAGTACT146000.019.87685212-13
Produced by FastQC (version 0.11.5)