##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138774_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5424507
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83667068730854	33.0	33.0	33.0	33.0	33.0
2	32.83452284235231	33.0	33.0	33.0	33.0	33.0
3	32.83411045464592	33.0	33.0	33.0	33.0	33.0
4	36.787174023372074	37.0	37.0	37.0	37.0	37.0
5	36.79866741807135	37.0	37.0	37.0	37.0	37.0
6	36.81025833315359	37.0	37.0	37.0	37.0	37.0
7	36.799271159572655	37.0	37.0	37.0	37.0	37.0
8	36.7950211881006	37.0	37.0	37.0	37.0	37.0
9	36.79089049014039	37.0	37.0	37.0	37.0	37.0
10-11	36.7695358306294	37.0	37.0	37.0	37.0	37.0
12-13	36.75568996408337	37.0	37.0	37.0	37.0	37.0
14-15	39.11381863826519	40.0	40.0	40.0	37.0	40.0
16-17	39.073846895210934	40.0	40.0	40.0	37.0	40.0
18-19	39.099844096431255	40.0	40.0	40.0	37.0	40.0
20-21	39.13834880248477	40.0	40.0	40.0	37.0	40.0
22-23	39.15734385363605	40.0	40.0	40.0	37.0	40.0
24-25	39.06756738995398	40.0	40.0	40.0	37.0	40.0
26-27	38.985376375146814	40.0	40.0	40.0	37.0	40.0
28-29	38.95267277944298	40.0	40.0	40.0	37.0	40.0
30-31	38.91305607021526	40.0	40.0	40.0	37.0	40.0
32-33	38.756746315586426	40.0	40.0	40.0	37.0	40.0
34-35	38.82878698133756	40.0	40.0	40.0	37.0	40.0
36-37	38.51237511632496	40.0	37.0	40.0	37.0	40.0
38-39	38.689220915	40.0	37.0	40.0	37.0	40.0
40-41	38.70888523413923	40.0	37.0	40.0	37.0	40.0
42-43	38.665304750958796	40.0	37.0	40.0	37.0	40.0
44-45	38.58833120263744	40.0	37.0	40.0	37.0	40.0
46-47	38.514308074654885	40.0	37.0	40.0	37.0	40.0
48-49	38.47112057078604	40.0	37.0	40.0	37.0	40.0
50-51	38.39382432038683	40.0	37.0	40.0	37.0	40.0
52-53	38.33728302725652	40.0	37.0	40.0	37.0	40.0
54-55	38.258734418909455	40.0	37.0	40.0	37.0	40.0
56-57	38.13315245554719	40.0	37.0	40.0	37.0	40.0
58-59	38.00459677196454	40.0	37.0	40.0	37.0	40.0
60-61	37.84764146459956	40.0	37.0	40.0	33.0	40.0
62-63	37.651997133754776	38.5	37.0	40.0	33.0	40.0
64-65	37.44018478458992	37.0	37.0	40.0	33.0	40.0
66-67	37.203889546632965	37.0	37.0	40.0	33.0	40.0
68-69	36.952560581793456	37.0	37.0	40.0	33.0	40.0
70-71	36.7012856768408	37.0	37.0	40.0	33.0	40.0
72-73	36.45289537081183	37.0	37.0	37.0	33.0	40.0
74-75	36.20127204397301	37.0	37.0	37.0	33.0	40.0
76-77	34.52902931763638	35.0	33.0	37.0	30.0	37.0
78-79	35.80185642026094	37.0	37.0	37.0	33.0	37.0
80-81	35.91672513268364	37.0	37.0	37.0	33.0	37.0
82-83	35.84043842954745	37.0	37.0	37.0	33.0	37.0
84-85	35.72923120321066	37.0	37.0	37.0	33.0	37.0
86-87	35.65368447958815	37.0	37.0	37.0	33.0	37.0
88-89	35.581299528794176	37.0	37.0	37.0	33.0	37.0
90-91	35.51877309544349	37.0	35.0	37.0	33.0	37.0
92-93	35.455358639241574	37.0	33.0	37.0	33.0	37.0
94-95	35.41376657550312	37.0	33.0	37.0	33.0	37.0
96-97	35.371723713267556	37.0	33.0	37.0	33.0	37.0
98-99	35.331891033196435	37.0	33.0	37.0	33.0	37.0
100	35.24739881936446	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	2.0
20	25.0
21	85.0
22	370.0
23	1029.0
24	2471.0
25	4984.0
26	8997.0
27	14362.0
28	21461.0
29	30051.0
30	40770.0
31	55532.0
32	74066.0
33	102139.0
34	147175.0
35	231774.0
36	470040.0
37	1531015.0
38	2573804.0
39	114350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.68208128076547	20.011886796696327	14.43871570978076	23.867316212757437
2	14.596573402260042	20.611425261581424	41.78911406863593	23.00288726752261
3	18.10768769043356	25.826514840913312	30.65814428854169	25.407653180111435
4	12.52788554421132	18.143899088210233	38.283646251900755	31.04456911567769
5	13.479298119867805	37.40878122592502	35.3916584123873	13.720262241819874
6	30.093855551086122	37.40860458076736	18.620368380088436	13.877171488058082
7	26.479148516012334	32.72038043648583	22.56000822932079	18.240462818181047
8	24.630279697358617	36.13838753243152	21.054857345535243	18.176475424674614
9	25.44189277327742	16.866604995920365	20.98869463873761	36.70280759206461
10-11	24.201031006230707	26.562213539609548	28.66534494889074	20.571410505269007
12-13	25.228569158450714	24.126893006129404	28.645119270746633	21.999418564673253
14-15	22.378890837453064	25.595100163019424	26.311211322982896	25.714797676544617
16-17	21.927918979549663	28.769425498022215	26.972829051561735	22.329826470866383
18-19	21.9588895359523	27.659942184607743	28.792487501629182	21.588680777810776
20-21	22.644524100623403	26.80148159509655	28.824774759495202	21.729219544784847
22-23	22.377149618389993	27.032822138680814	28.67243115307826	21.917597089850936
24-25	22.35385379413717	27.17675557921754	28.629425841776442	21.839964784868855
26-27	22.21577401852321	27.355916175115453	28.63524430038364	21.793065505977697
28-29	22.015074856197085	27.195018046127384	28.697719932155945	22.092187165519587
30-31	22.552917401470093	27.119427595602403	28.60585504681697	21.721799956110534
32-33	22.357021952437588	27.22898689845875	28.622450330598515	21.791540818505148
34-35	22.28495846527247	27.327580814866494	28.78294348764018	21.60451723222086
36-37	22.384320931971637	27.244366646006114	28.487297299764496	21.88401512225775
38-39	22.058845699452473	27.11840426072821	28.713321395808357	22.109428644010965
40-41	22.270028888104708	26.952358648843827	28.70703829383647	22.070574169215
42-43	22.335979083580995	26.983191364049674	28.52296281924835	22.15786673312098
44-45	22.20398760411362	27.268053442992148	28.297490948626397	22.230468004267838
46-47	22.541825783348116	27.101797236908432	27.79355435595666	22.562822623786797
48-49	22.339561672275522	27.26657259523078	27.835430277530204	22.558435454963494
50-51	22.304016822505282	27.50357361479685	27.770801214788648	22.42160834790922
52-53	22.419610151565564	27.476736399085393	27.823157007677736	22.280496441671303
54-55	22.321809985592	27.467185869569978	27.63894523407327	22.572058910764756
56-57	22.564849513039654	27.64558403677737	27.36163046935288	22.4279359808301
58-59	22.24486929818939	27.912342248012212	27.662022868551546	22.18076558524685
60-61	22.526899140472057	28.012299610970086	27.286024606374543	22.174776642183318
62-63	22.315176427528566	28.240130413649993	27.22047709639664	22.224216062424798
64-65	22.37374438658211	28.05579256392898	27.222593692555275	22.34786935693364
66-67	22.42326633688858	27.90238214731633	27.184305748867203	22.490045766927885
68-69	22.467516206598365	27.688696913300358	27.31441200431146	22.529374875789816
70-71	22.4718440637697	27.451280155885417	27.40254174014124	22.674334040203647
72-73	22.587743276640246	27.40060522574016	27.304089088742025	22.70756240887757
74-75	22.458921523722804	27.562762097271857	27.266004218240464	22.712312160764878
76-77	22.60852474554651	27.50238065710565	27.2155391579339	22.673555439413935
78-79	22.580206228215083	27.48835948222127	27.16319086312294	22.768243426440705
80-81	22.61368490225998	27.512835943375325	27.19641803495091	22.677061119413786
82-83	22.696469422866734	27.424251194789882	27.276625755124673	22.602653627218704
84-85	22.775230567386355	27.413858981237144	27.199952547299507	22.610957904076997
86-87	22.636120830417543	27.44862374620947	27.283834849545137	22.63142057382785
88-89	22.658831590075962	27.37974826101749	27.2614909641307	22.699929184775847
90-91	22.70339397928681	27.3918014138984	27.246784969429918	22.65801963738488
92-93	22.800234689025555	27.266362492989373	27.352889865537406	22.580512952447666
94-95	22.67870528117379	27.308029809957052	27.358393574808996	22.654871334060157
96-97	22.77081458836422	27.44718846672266	27.38485910520188	22.397137839711238
98-99	23.896779281266607	29.34272874597104	27.537686350811047	19.2228056219513
100	22.722289985281012	30.326211837238326	21.423250898509828	25.528247278970834
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	828.0
1	788.0
2	1146.0
3	1699.0
4	2408.0
5	4029.0
6	5470.0
7	6523.5
8	7414.0
9	7655.5
10	7373.0
11	7048.5
12	7681.5
13	9963.5
14	15283.0
15	22561.0
16	28649.5
17	32670.5
18	34009.0
19	32459.0
20	29454.5
21	26166.0
22	24137.5
23	24746.5
24	27621.0
25	33162.5
26	41101.5
27	50122.5
28	60734.5
29	72695.0
30	84330.5
31	96089.5
32	108111.5
33	119857.0
34	131678.0
35	142347.0
36	153273.0
37	164674.0
38	174112.0
39	181900.5
40	190546.5
41	198073.5
42	202427.0
43	206769.5
44	210646.0
45	215366.0
46	222162.5
47	228800.0
48	236244.5
49	234779.0
50	226770.5
51	221075.0
52	213002.5
53	200444.0
54	188077.0
55	176771.0
56	163721.5
57	149635.0
58	134380.5
59	117147.5
60	97512.5
61	79529.5
62	63452.0
63	49190.5
64	37387.0
65	27966.5
66	20773.5
67	15672.0
68	11953.0
69	8484.0
70	5834.5
71	4165.0
72	2855.5
73	1874.5
74	1225.0
75	786.5
76	507.5
77	316.5
78	203.0
79	134.5
80	86.5
81	53.5
82	34.5
83	20.0
84	12.5
85	11.0
86	8.5
87	7.5
88	6.0
89	3.5
90	2.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.8434855001569727E-5
2	1.8434855001569727E-5
3	7.926987650674983E-4
4	0.01839798529156659
5	8.848730400753469E-4
6	1.8434855001569727E-5
7	1.8434855001569727E-5
8	1.8434855001569727E-5
9	1.8434855001569727E-5
10-11	9.217427500784864E-6
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	2.7652697967738702E-5
22-23	0.0
24-25	0.007781540084796659
26-27	0.051105284356498144
28-29	0.08496369573622131
30-31	0.07528931797816729
32-33	0.03524450225562963
34-35	0.004027380246683536
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.384017286356538E-4
56-57	7.025274831260425E-4
58-59	2.0250734468825766E-5
60-61	2.0435995840457405E-5
62-63	2.0625928875815718E-5
64-65	2.0833352864601645E-5
66-67	0.0
68-69	0.0
70-71	6.459693076296405E-5
72-73	0.013051886410551923
74-75	0.028142935843428758
76-77	0.003908554008853263
78-79	0.0
80-81	0.0
82-83	0.010719677354835705
84-85	0.05001264734036179
86-87	0.0407461619039125
88-89	0.010317556708239542
90-91	1.4324949298632575E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	48.0
20-21	159.0
22-23	505.0
24-25	2165.0
26-27	1915.0
28-29	4289.0
30-31	10180.0
32-33	13044.0
34-35	18335.0
36-37	26420.0
38-39	31410.0
40-41	33861.0
42-43	40319.0
44-45	48018.0
46-47	46580.0
48-49	41649.0
50-51	36989.0
52-53	35436.0
54-55	39926.0
56-57	43926.0
58-59	44738.0
60-61	45061.0
62-63	47142.0
64-65	51638.0
66-67	55519.0
68-69	49788.0
70-71	44095.0
72-73	41838.0
74-75	43187.0
76-77	42984.0
78-79	45650.0
80-81	46495.0
82-83	45070.0
84-85	45658.0
86-87	47719.0
88-89	50720.0
90-91	52989.0
92-93	54809.0
94-95	60758.0
96-97	174076.0
98-99	329944.0
100-101	3529455.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.90161488195679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.48376386588782	80.41570579291147
2	6.2769205108338735	11.035028987759494
3	1.3033299492287056	3.43694421783666
4	0.44319646612992525	1.5583074033118758
5	0.1906837021548968	0.8380702675542747
6	0.09805770991897284	0.517165863210251
7	0.05790502230640967	0.35629614793563985
8	0.03583953214763296	0.2520282201912594
9	0.021944382918559328	0.1736052026516454
>10	0.0858906969411615	1.1996837186373128
>50	0.0019648963189182974	0.11654517442963713
>100	4.721947896915923E-4	0.0843960296108487
>500	3.1070423460582875E-5	0.01622297395967374
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	1.8434855001569727E-5	0.0	0.0	0.0
9	0.0	1.8434855001569727E-5	0.0	0.0	0.0
10-11	0.0	2.765228250235459E-5	0.0	1.8434855001569727E-5	0.0
12-13	0.0	3.6869710003139454E-5	0.0	1.8434855001569727E-5	0.0
14-15	0.0	1.1060913000941836E-4	0.0	2.765228250235459E-5	0.0
16-17	0.0	1.3826141251177296E-4	0.0	4.608713750392432E-5	0.0
18-19	0.0	1.6591369501412753E-4	0.0	8.295684750706377E-5	0.0
20-21	9.217427500784864E-6	1.8434855001569727E-4	0.0	1.566962675133427E-4	0.0
22-23	1.8434855001569727E-5	2.2121826001883673E-4	0.0	5.069585125431675E-4	0.0
24-25	1.8434855001569727E-5	2.580879700219762E-4	0.0	0.0012996572776106659	0.0
26-27	3.6869710003139454E-5	2.765228250235459E-4	0.0	0.003935841542835137	0.0
28-29	3.6869710003139454E-5	2.765228250235459E-4	0.0	0.01229604828604701	0.0
30-31	3.6869710003139454E-5	3.2260996252747023E-4	0.0	0.013641792701161597	0.0
32-33	3.6869710003139454E-5	3.5026224502982483E-4	0.0	0.01619502011887901	0.0
34-35	4.608713750392432E-5	4.7008880254002805E-4	0.0	0.019808251699186673	0.0
36-37	5.530456500470918E-5	5.899153600502313E-4	0.0	0.02520044678714582	0.0
38-39	5.530456500470918E-5	5.899153600502313E-4	0.0	0.03888832662581134	0.0
40-41	5.530456500470918E-5	6.820896350580799E-4	0.0	0.05353481892455849	0.0
42-43	5.530456500470918E-5	7.005244900596497E-4	0.0	0.06352651033540929	9.217427500784864E-6
44-45	7.373942000627891E-5	8.480033300722076E-4	0.0	0.07579490633895394	1.8434855001569727E-5
46-47	9.217427500784864E-5	9.770473150831956E-4	0.0	0.08919704592509513	1.8434855001569727E-5
48-49	9.217427500784864E-5	9.770473150831956E-4	0.0	0.10137326765363194	1.8434855001569727E-5
50-51	9.217427500784864E-5	0.0010323518800879048	0.0	0.1136969682221813	1.8434855001569727E-5
52-53	9.217427500784864E-5	0.0010507867350894745	0.0	0.14246455945213088	1.8434855001569727E-5
54-55	9.217427500784864E-5	0.0010692215900910441	0.0	0.17920522547025933	1.8434855001569727E-5
56-57	9.217427500784864E-5	0.001087656445092614	0.0	0.21614867489340506	1.8434855001569727E-5
58-59	9.217427500784864E-5	0.001087656445092614	0.0	0.2396899847304096	1.8434855001569727E-5
60-61	9.217427500784864E-5	0.0011060913000941837	0.0	0.2559679616967957	1.8434855001569727E-5
62-63	1.013917025086335E-4	0.0011060913000941837	0.0	0.2690382738929086	1.8434855001569727E-5
64-65	1.1060913000941836E-4	0.0011982655751020324	0.0	0.2759236922359949	1.8434855001569727E-5
66-67	1.1060913000941836E-4	0.0011982655751020324	0.0	0.2826247620290655	2.765228250235459E-5
68-69	1.1060913000941836E-4	0.0011982655751020324	0.0	0.2894364409521455	3.6869710003139454E-5
70-71	1.1060913000941836E-4	0.0012074830026028173	0.0	0.2944230692300701	3.6869710003139454E-5
72-73	1.1060913000941836E-4	0.001216700430103602	0.0	0.29807317052038096	3.6869710003139454E-5
74-75	1.1060913000941836E-4	0.0012351352851051717	0.0	0.30086605105311875	3.6869710003139454E-5
76-77	1.1060913000941836E-4	0.001281222422609096	0.0	0.30400919383088637	3.6869710003139454E-5
78-79	1.1060913000941836E-4	0.001290439850109881	0.0	0.30623981128607636	3.6869710003139454E-5
80-81	1.1060913000941836E-4	0.001290439850109881	0.0	0.3067928569361234	5.530456500470918E-5
82-83	1.1060913000941836E-4	0.0012996572776106659	0.0	0.30730903287616734	7.373942000627891E-5
84-85	1.1060913000941836E-4	0.0013088747051114506	0.0	0.3075302511361862	7.373942000627891E-5
86-87	1.1060913000941836E-4	0.0013273095601130205	0.0	0.3076132079836933	7.373942000627891E-5
88	1.1060913000941836E-4	0.001419483835120869	0.0	0.30762242541119406	7.373942000627891E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7160	0.0	51.67591	1
GTATCAA	13265	0.0	38.53711	1
CTTATAC	6070	0.0	32.019123	1
TCAACGC	16360	0.0	30.648441	4
ATCAACG	16575	0.0	30.251171	3
CAACGCA	16820	0.0	29.85532	5
TATCAAC	17010	0.0	29.702572	2
AACGCAG	17290	0.0	29.043753	6
TATACAC	8145	0.0	26.733807	3
ACGCAGA	19655	0.0	25.613945	7
CGCAGAG	19945	0.0	25.220201	8
TTATACA	8315	0.0	24.345943	2
GTGGTAT	3470	0.0	22.306108	1
TGGTATC	3285	0.0	21.87929	2
GCAGAGT	23155	0.0	21.61372	9
GTACATG	15675	0.0	20.538572	1
TACATGG	15625	0.0	20.250458	2
ACACATC	9755	0.0	20.094236	6
ACATCTC	9955	0.0	19.94681	8
GAGTACT	14600	0.0	19.876852	12-13
>>END_MODULE