FastQCFastQC Report
Fri 27 May 2016
SRR2138773_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138773_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5007747
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71650.051.2146261
GTATCAA133050.039.285561
CTTATAC45550.032.692551
TCAACGC161950.031.6163734
ATCAACG163850.031.1976683
CAACGCA165950.030.8726165
TATCAAC168750.030.5193482
AACGCAG168700.030.4705056
ACGCAGA191500.026.8426847
TATACAC64400.026.5686973
CGCAGAG194950.026.3457728
TTATACA65000.024.6166922
GCAGAGT227300.022.5210959
GTGGTAT34650.022.0425361
TGGTATC33550.021.4934392
GTACATG157550.021.178741
GAGTACT138350.020.93622212-13
TACATGG159400.020.3708022
TACACAT84150.020.1760715
TATACCG2602.39379E-919.6886335
Produced by FastQC (version 0.11.5)