##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138773_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5007747
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83810084654836	33.0	33.0	33.0	33.0	33.0
2	32.834033947801274	33.0	33.0	33.0	33.0	33.0
3	32.833443063317695	33.0	33.0	33.0	33.0	33.0
4	36.78858496645298	37.0	37.0	37.0	37.0	37.0
5	36.798923947236155	37.0	37.0	37.0	37.0	37.0
6	36.8092725131681	37.0	37.0	37.0	37.0	37.0
7	36.79944733629714	37.0	37.0	37.0	37.0	37.0
8	36.79616182686546	37.0	37.0	37.0	37.0	37.0
9	36.79033685208139	37.0	37.0	37.0	37.0	37.0
10-11	36.77011178879444	37.0	37.0	37.0	37.0	37.0
12-13	36.755885830494236	37.0	37.0	37.0	37.0	37.0
14-15	39.111981495870296	40.0	40.0	40.0	37.0	40.0
16-17	39.07311421683244	40.0	40.0	40.0	37.0	40.0
18-19	39.10077975185248	40.0	40.0	40.0	37.0	40.0
20-21	39.14025726413088	40.0	40.0	40.0	37.0	40.0
22-23	39.15826278348591	40.0	40.0	40.0	37.0	40.0
24-25	39.06738019924205	40.0	40.0	40.0	37.0	40.0
26-27	38.99036465687165	40.0	40.0	40.0	37.0	40.0
28-29	38.96773887513413	40.0	40.0	40.0	37.0	40.0
30-31	38.92638036128403	40.0	40.0	40.0	37.0	40.0
32-33	38.7741912240555	40.0	40.0	40.0	37.0	40.0
34-35	38.844457940790775	40.0	40.0	40.0	37.0	40.0
36-37	38.5288381352343	40.0	37.0	40.0	37.0	40.0
38-39	38.71284704205987	40.0	37.0	40.0	37.0	40.0
40-41	38.72271405144241	40.0	37.0	40.0	37.0	40.0
42-43	38.67238878882952	40.0	37.0	40.0	37.0	40.0
44-45	38.58576400545864	40.0	37.0	40.0	37.0	40.0
46-47	38.50802353369224	40.0	37.0	40.0	37.0	40.0
48-49	38.4597915007575	40.0	37.0	40.0	37.0	40.0
50-51	38.39476704047784	40.0	37.0	40.0	37.0	40.0
52-53	38.340386410666305	40.0	37.0	40.0	37.0	40.0
54-55	38.24927299100915	40.0	37.0	40.0	37.0	40.0
56-57	38.11548851802962	40.0	37.0	40.0	37.0	40.0
58-59	37.98337197501195	40.0	37.0	40.0	37.0	40.0
60-61	37.81936328425872	40.0	37.0	40.0	33.0	40.0
62-63	37.62623478026668	38.5	37.0	40.0	33.0	40.0
64-65	37.40308551270002	37.0	37.0	40.0	33.0	40.0
66-67	37.16068694651066	37.0	37.0	40.0	33.0	40.0
68-69	36.90410430462498	37.0	37.0	40.0	33.0	40.0
70-71	36.64984140901035	37.0	37.0	40.0	33.0	40.0
72-73	36.39971842827315	37.0	37.0	37.0	33.0	40.0
74-75	36.15236084056295	37.0	37.0	37.0	33.0	40.0
76-77	34.47069527734152	35.0	33.0	37.0	30.0	37.0
78-79	35.76727845431543	37.0	35.0	37.0	33.0	37.0
80-81	35.88994946726284	37.0	37.0	37.0	33.0	37.0
82-83	35.81756800793111	37.0	37.0	37.0	33.0	37.0
84-85	35.70810322937244	37.0	37.0	37.0	33.0	37.0
86-87	35.63177079174075	37.0	37.0	37.0	33.0	37.0
88-89	35.56049879084467	37.0	37.0	37.0	33.0	37.0
90-91	35.49631839567027	37.0	35.0	37.0	33.0	37.0
92-93	35.43260350253843	37.0	33.0	37.0	33.0	37.0
94-95	35.387479248773516	37.0	33.0	37.0	33.0	37.0
96-97	35.34833890259033	37.0	33.0	37.0	33.0	37.0
98-99	35.30221899263811	37.0	33.0	37.0	33.0	37.0
100	35.21028952126301	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	73.0
22	281.0
23	870.0
24	2120.0
25	4289.0
26	7916.0
27	13021.0
28	19228.0
29	27481.0
30	38279.0
31	51304.0
32	68766.0
33	94531.0
34	137523.0
35	216088.0
36	440923.0
37	1447048.0
38	2340664.0
39	97334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.00913105234749	19.833410114368796	14.354419262794227	23.803039570489485
2	14.768557596859427	20.62889758608013	41.55134035325666	23.05120446380378
3	18.153952021515654	25.864553069541063	30.4620901039858	25.519404804957485
4	12.61761221145711	17.992481287105218	38.14304728775874	31.246859213678928
5	13.594853050419914	37.310272332390255	35.191876356909276	13.90299826028056
6	30.209633194328706	37.25909076476906	18.58376631247545	13.947509728426777
7	26.73583549648175	32.63401685428597	22.431045338352757	18.19910231087952
8	24.74104622298211	35.991335025511475	20.95571122103413	18.311907530472286
9	25.642090145528517	16.85222915614547	20.85684440527846	36.64883629304755
10-11	24.315695261761427	26.486152355540327	28.495489089205183	20.70266329349306
12-13	25.361055580483598	24.101447217680924	28.515058767944947	22.022438433890528
14-15	22.516443023179885	25.458604438283324	26.266682402285895	25.758270136250893
16-17	22.035368400200728	28.687581461283884	26.846184521702078	22.430865616813307
18-19	22.015039897183307	27.54345417210574	28.761876348785194	21.679629581925763
20-21	22.75690606907342	26.57166520972693	28.837601725762173	21.833826995437473
22-23	22.457041490902537	26.714912869753725	28.692544538701227	22.135501100642514
24-25	22.293949148096676	26.94442296931694	28.74688535737908	22.014742525207303
26-27	22.15870826179641	26.98976116038516	28.754350319760686	22.097180258057744
28-29	21.921524280306073	26.83472339543399	28.857110927265783	22.386641396994154
30-31	22.222431416597658	26.68621988690084	28.8526735591985	22.238675137303005
32-33	22.020015122483493	26.987714837868644	28.759510984865276	22.23275905478259
34-35	22.142234591634622	27.167993160137655	28.85919818820862	21.83057406001911
36-37	22.35363284325469	27.035805604402857	28.36502627864833	22.245535273694124
38-39	22.123640044711415	26.969807623255615	28.638147924996805	22.268404407036165
40-41	22.34783020383042	26.87032974926632	28.57212386427849	22.209716182624774
42-43	22.40416220925223	26.914273150219653	28.40512487726599	22.27643976326213
44-45	22.340431369402435	27.130086372236644	28.143413631645835	22.386068626715087
46-47	22.643583689204323	27.01692766775226	27.68414854045434	22.655340102589076
48-49	22.508461159637235	27.171031663150696	27.645347914681693	22.675159262530375
50-51	22.44673452614276	27.402884780389996	27.575616704290617	22.574763989176628
52-53	22.553096683186848	27.36963150301543	27.61392429309827	22.46334752069945
54-55	22.49547900945582	27.341355801416974	27.459655952080738	22.703509237046475
56-57	22.697947672843974	27.51845527869695	27.229161160040192	22.55443588841888
58-59	22.37080175977074	27.714789520173884	27.52127784756995	22.39313087248543
60-61	22.68163673217314	27.812146171810433	27.18105734239885	22.32515975361758
62-63	22.458693722427167	28.024480437507528	27.132001659388255	22.38482418067705
64-65	22.497576933078324	27.887806091607125	27.080443570401773	22.534173404912774
66-67	22.557900904322533	27.76438407210284	27.04417238444246	22.633542639132166
68-69	22.578195868247704	27.610888627582348	27.131524340497627	22.679391163672328
70-71	22.59184173819045	27.37065122706394	27.21718697471578	22.82032006002983
72-73	22.663591223624856	27.3427284205821	27.11598263204806	22.877697723744983
74-75	22.570913360969254	27.515277519744842	27.097547796761123	22.816261322524785
76-77	22.689180620162155	27.42535580802514	27.058672335926794	22.82679123588591
78-79	22.602481624835015	27.463282756762027	27.018585115304234	22.91565050309872
80-81	22.633976912414536	27.50236202562652	27.007940488618537	22.85572057334041
82-83	22.696584951599284	27.456814879538182	27.091146498065932	22.755453670796598
84-85	22.78270842558154	27.451852013461252	27.02767966893285	22.73775989202435
86-87	22.689397771125183	27.47199133449047	27.08904432009503	22.749566574289318
88-89	22.711128812571925	27.444459523570718	27.042432872135514	22.80197879172184
90-91	22.70219811237383	27.482588306703637	27.039310645685198	22.775902935237337
92-93	22.740987476027424	27.419062630215414	27.094368482759158	22.74558141099801
94-95	22.597112828044388	27.471510482074656	27.162302613792622	22.76907407608833
96-97	22.750595765761418	27.547547216420696	27.15217963701572	22.549677380802162
98-99	23.85795166609838	29.522286108426123	27.318291504502053	19.301470720973455
100	22.755174823227094	30.413840540484898	21.193130833142604	25.6378538031454
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1338.0
1	1173.5
2	1444.5
3	2004.0
4	2719.0
5	4387.5
6	5777.5
7	6753.5
8	7482.5
9	7548.5
10	7225.5
11	6843.0
12	7457.5
13	9715.0
14	14846.5
15	21864.5
16	28011.0
17	32060.0
18	32868.0
19	30931.0
20	27715.0
21	23999.0
22	21480.5
23	21583.0
24	24082.0
25	29059.5
26	35736.5
27	43349.0
28	52220.5
29	62381.0
30	73033.5
31	83717.5
32	94770.0
33	105367.0
34	116136.5
35	126552.5
36	137415.5
37	148550.0
38	157552.5
39	165358.0
40	172885.0
41	179753.0
42	184113.5
43	188506.5
44	195039.5
45	203514.0
46	210820.0
47	215533.0
48	215480.0
49	211646.5
50	208319.0
51	202000.5
52	193968.5
53	185521.0
54	176036.5
55	165481.5
56	153588.5
57	140716.5
58	126741.5
59	110873.5
60	93040.0
61	76087.0
62	61217.5
63	47889.0
64	36347.0
65	27095.5
66	20261.5
67	15522.5
68	11873.5
69	8612.5
70	6159.5
71	4392.0
72	2995.5
73	2030.5
74	1396.0
75	923.0
76	605.0
77	382.5
78	240.5
79	154.5
80	105.0
81	78.5
82	56.0
83	33.0
84	20.5
85	16.5
86	13.5
87	12.5
88	10.0
89	6.5
90	3.5
91	2.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	8.786386372953746E-4
4	0.02074785327613396
5	8.586695773568433E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.9969472667133753E-5
22-23	0.0
24-25	0.0077104744798276205
26-27	0.05180461915362143
28-29	0.08634757648502987
30-31	0.07634324177681516
32-33	0.03636859890850084
34-35	0.004704999071566957
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.478725262726018E-4
56-57	8.039202628428163E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.321697681065139E-5
72-73	0.013586267469333979
74-75	0.029903179153360777
76-77	0.004220055635592235
78-79	0.0
80-81	0.0
82-83	0.011264414585429051
84-85	0.0515291290734681
86-87	0.041860257321071175
88-89	0.011027766322137495
90-91	1.2789298733335066E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	61.0
20-21	259.0
22-23	643.0
24-25	1954.0
26-27	2138.0
28-29	3967.0
30-31	9515.0
32-33	12088.0
34-35	16764.0
36-37	23970.0
38-39	28613.0
40-41	30530.0
42-43	37024.0
44-45	44304.0
46-47	43740.0
48-49	38064.0
50-51	33613.0
52-53	32474.0
54-55	35787.0
56-57	38643.0
58-59	40181.0
60-61	39964.0
62-63	41074.0
64-65	44137.0
66-67	47461.0
68-69	43592.0
70-71	38941.0
72-73	37043.0
74-75	37676.0
76-77	37791.0
78-79	40387.0
80-81	40845.0
82-83	38944.0
84-85	40001.0
86-87	41208.0
88-89	43451.0
90-91	45552.0
92-93	46975.0
94-95	55957.0
96-97	162023.0
98-99	307285.0
100-101	3303108.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.32634908495866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.78606923157459	81.0712839208424
2	6.096990022852868	10.77049738252025
3	1.2334016710251372	3.268255996708129
4	0.42924108014124424	1.5165318994464103
5	0.1735244776028829	0.7663391791768664
6	0.09218659718228253	0.48855033382060536
7	0.05216622852524429	0.32253567578165193
8	0.03372298144765988	0.23829022652252732
9	0.021581756792113042	0.17156140058581687
>10	0.07836899097015597	1.1007014004549782
>50	0.0019431254993333156	0.11386317063187344
>100	7.70605603278744E-4	0.1564440201140975
>500	3.323078321494873E-5	0.015145393394428358
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.987623975412497E-5	0.0	0.0	0.0
3	0.0	7.987623975412497E-5	0.0	0.0	0.0
4	0.0	9.98452996926562E-5	0.0	0.0	0.0
5	1.996905993853124E-5	1.1981435963118743E-4	0.0	0.0	0.0
6	3.993811987706248E-5	1.1981435963118743E-4	0.0	0.0	0.0
7	3.993811987706248E-5	1.1981435963118743E-4	0.0	0.0	0.0
8	3.993811987706248E-5	1.397834195697187E-4	0.0	0.0	0.0
9	3.993811987706248E-5	3.1950495901649986E-4	0.0	0.0	0.0
10-11	3.993811987706248E-5	3.1950495901649986E-4	0.0	0.0	0.0
12-13	3.993811987706248E-5	3.1950495901649986E-4	0.0	0.0	0.0
14-15	3.993811987706248E-5	4.19350258709156E-4	0.0	0.0	9.98452996926562E-6
16-17	4.9922649846328103E-5	4.19350258709156E-4	0.0	0.0	1.996905993853124E-5
18-19	5.990717981559372E-5	4.2933478867842164E-4	0.0	0.0	1.996905993853124E-5
20-21	5.990717981559372E-5	4.3931931864768726E-4	0.0	0.0	1.996905993853124E-5
22-23	5.990717981559372E-5	4.692729085554841E-4	0.0	2.9953589907796862E-5	1.996905993853124E-5
24-25	5.990717981559372E-5	5.191955584018123E-4	0.0	3.993811987706248E-5	1.996905993853124E-5
26-27	5.990717981559372E-5	5.191955584018123E-4	0.0	8.986076972339059E-5	1.996905993853124E-5
28-29	5.990717981559372E-5	5.191955584018123E-4	0.0	2.795668391394373E-4	1.996905993853124E-5
30-31	7.987623975412497E-5	5.591336782788748E-4	0.0	8.886231672646402E-4	1.996905993853124E-5
32-33	7.987623975412497E-5	5.591336782788748E-4	0.0	0.00241625625256228	1.996905993853124E-5
34-35	7.987623975412497E-5	6.689635079407965E-4	0.0	0.00467276002561631	1.996905993853124E-5
36-37	7.987623975412497E-5	7.787933376027183E-4	0.0	0.008347067054306058	1.996905993853124E-5
38-39	7.987623975412497E-5	7.987623975412495E-4	0.0	0.016963716417782288	1.996905993853124E-5
40-41	7.987623975412497E-5	8.886231672646401E-4	0.0	0.026019685099906206	1.996905993853124E-5
42-43	7.987623975412497E-5	9.285612871417026E-4	0.0	0.03297890248848434	1.996905993853124E-5
44-45	8.986076972339059E-5	0.0010483756467728903	0.0	0.04133595407275967	1.996905993853124E-5
46-47	9.98452996926562E-5	0.0011182673565577495	0.0	0.05014230950565194	2.9953589907796862E-5
48-49	9.98452996926562E-5	0.0011282518865270152	0.0	0.05817985613091077	3.993811987706248E-5
50-51	1.1981435963118743E-4	0.001238081716188937	0.0	0.06597777403690722	3.993811987706248E-5
52-53	1.1981435963118743E-4	0.001238081716188937	0.0	0.08448909259992568	3.993811987706248E-5
54-55	1.1981435963118743E-4	0.0012480662461582027	0.0	0.10738361981945174	3.993811987706248E-5
56-57	1.1981435963118743E-4	0.0012580507761274682	0.0	0.1313564762756585	3.993811987706248E-5
58-59	1.1981435963118743E-4	0.0012580507761274682	0.0	0.15289310741936446	3.993811987706248E-5
60-61	1.2979888960045307E-4	0.0012680353060967337	0.0	0.1634168020069704	3.993811987706248E-5
62-63	1.5975247950824993E-4	0.0012780198360659994	0.0	0.1750288103612263	5.990717981559372E-5
64-65	1.5975247950824993E-4	0.0014577413755127804	0.0	0.18067006979386138	5.990717981559372E-5
66-67	1.7972153944678115E-4	0.0014876949654205772	0.0	0.18666078777542078	5.990717981559372E-5
68-69	1.996905993853124E-4	0.0015875402651132336	0.0	0.19235196985790215	5.990717981559372E-5
70-71	1.996905993853124E-4	0.0016174938550210303	0.0	0.19714454424314967	5.990717981559372E-5
72-73	1.996905993853124E-4	0.001647447444928827	0.0	0.20043943913300732	5.990717981559372E-5
74-75	1.996905993853124E-4	0.0016973700947751553	0.0	0.20348472077363333	5.990717981559372E-5
76-77	1.996905993853124E-4	0.0017772463345292803	0.0	0.20635028087481255	5.990717981559372E-5
78-79	1.996905993853124E-4	0.0018171844544063427	0.0	0.20815748079924964	5.990717981559372E-5
80-81	1.996905993853124E-4	0.0018171844544063427	0.0	0.20891630507691383	5.990717981559372E-5
82-83	1.996905993853124E-4	0.0018171844544063427	0.0	0.209185887386084	5.990717981559372E-5
84-85	1.996905993853124E-4	0.0018171844544063427	0.0	0.2094754387551927	5.990717981559372E-5
86-87	1.996905993853124E-4	0.0018571225742834052	0.0	0.2095852685848546	5.990717981559372E-5
88	1.996905993853124E-4	0.0020368441137301866	0.0	0.20959525311482388	5.990717981559372E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7165	0.0	51.214626	1
GTATCAA	13305	0.0	39.28556	1
CTTATAC	4555	0.0	32.69255	1
TCAACGC	16195	0.0	31.616373	4
ATCAACG	16385	0.0	31.197668	3
CAACGCA	16595	0.0	30.872616	5
TATCAAC	16875	0.0	30.519348	2
AACGCAG	16870	0.0	30.470505	6
ACGCAGA	19150	0.0	26.842684	7
TATACAC	6440	0.0	26.568697	3
CGCAGAG	19495	0.0	26.345772	8
TTATACA	6500	0.0	24.616692	2
GCAGAGT	22730	0.0	22.521095	9
GTGGTAT	3465	0.0	22.042536	1
TGGTATC	3355	0.0	21.493439	2
GTACATG	15755	0.0	21.17874	1
GAGTACT	13835	0.0	20.936222	12-13
TACATGG	15940	0.0	20.370802	2
TACACAT	8415	0.0	20.176071	5
TATACCG	260	2.39379E-9	19.688633	5
>>END_MODULE