FastQCFastQC Report
Fri 27 May 2016
SRR2138772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5906337
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90350.051.8157231
GTATCAA167650.037.739991
CTTATAC47600.036.893131
ATCAACG197600.031.3736923
TCAACGC205950.030.0802734
TATCAAC211600.029.640042
CAACGCA211800.029.2261525
AACGCAG213700.029.0060946
ACGCAGA215250.028.757727
TATACAC70300.027.8225923
CGCAGAG250850.024.6934458
TTATACA72550.024.4399932
ACACATC80800.022.2043116
GTGGTAT43550.021.9683151
GCAGAGT287850.021.50469
GAGTACT177950.020.78535812-13
TGGTATC43500.020.625082
GTACATG186350.020.1252631
ACATCTC86450.020.0646428
TACACAT98050.019.642055
Produced by FastQC (version 0.11.5)