##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138772_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5906337
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89432705922469	33.0	33.0	33.0	33.0	33.0
2	32.95176976864002	33.0	33.0	33.0	33.0	33.0
3	33.6223781338586	33.0	33.0	33.0	33.0	37.0
4	36.788062042514674	37.0	37.0	37.0	37.0	37.0
5	36.80236887939174	37.0	37.0	37.0	37.0	37.0
6	36.805956720722165	37.0	37.0	37.0	37.0	37.0
7	36.80053508629799	37.0	37.0	37.0	37.0	37.0
8	36.786840981136024	37.0	37.0	37.0	37.0	37.0
9	36.788981563361524	37.0	37.0	37.0	37.0	37.0
10-11	36.78211334707112	37.0	37.0	37.0	37.0	37.0
12-13	37.03633902027602	37.0	37.0	37.0	37.0	38.5
14-15	39.10778372449794	40.0	40.0	40.0	37.0	40.0
16-17	39.06634535076478	40.0	40.0	40.0	37.0	40.0
18-19	39.10191519718567	40.0	40.0	40.0	37.0	40.0
20-21	39.1406043168993	40.0	40.0	40.0	37.0	40.0
22-23	39.156984941841635	40.0	40.0	40.0	37.0	40.0
24-25	39.04850737971023	40.0	40.0	40.0	37.0	40.0
26-27	38.9838410611883	40.0	40.0	40.0	37.0	40.0
28-29	38.95725964730548	40.0	40.0	40.0	37.0	40.0
30-31	38.89203980350272	40.0	40.0	40.0	37.0	40.0
32-33	38.77267779110498	40.0	38.5	40.0	37.0	40.0
34-35	38.790079387766525	40.0	37.0	40.0	37.0	40.0
36-37	38.555036554357315	40.0	37.0	40.0	37.0	40.0
38-39	38.697853462453374	40.0	37.0	40.0	37.0	40.0
40-41	38.71090936903062	40.0	37.0	40.0	37.0	40.0
42-43	38.65767411636098	40.0	37.0	40.0	37.0	40.0
44-45	38.576677461990386	40.0	37.0	40.0	37.0	40.0
46-47	38.50494582109422	40.0	37.0	40.0	37.0	40.0
48-49	38.45719780209068	40.0	37.0	40.0	37.0	40.0
50-51	38.39340388087298	40.0	37.0	40.0	37.0	40.0
52-53	38.33960765854722	40.0	37.0	40.0	37.0	40.0
54-55	38.24690519025741	40.0	37.0	40.0	37.0	40.0
56-57	38.120493062681874	40.0	37.0	40.0	37.0	40.0
58-59	37.99729984952539	40.0	37.0	40.0	37.0	40.0
60-61	37.833352431981396	40.0	37.0	40.0	33.0	40.0
62-63	37.64137809799311	38.5	37.0	40.0	33.0	40.0
64-65	37.42838152134388	37.0	37.0	40.0	33.0	40.0
66-67	37.18524999862498	37.0	37.0	40.0	33.0	40.0
68-69	36.9308441655125	37.0	37.0	40.0	33.0	40.0
70-71	36.678905852183064	37.0	37.0	40.0	33.0	40.0
72-73	36.42098197246022	37.0	37.0	37.0	33.0	40.0
74-75	35.97072901759287	37.0	35.0	37.0	33.0	40.0
76-77	34.7142352658421	37.0	33.0	37.0	30.0	37.0
78-79	35.822989992379995	37.0	37.0	37.0	33.0	37.0
80-81	35.91338269948264	37.0	37.0	37.0	33.0	37.0
82-83	35.828765689756935	37.0	37.0	37.0	33.0	37.0
84-85	35.71924813449637	37.0	37.0	37.0	33.0	37.0
86-87	35.64063328467298	37.0	37.0	37.0	33.0	37.0
88-89	35.567945390315955	37.0	37.0	37.0	33.0	37.0
90-91	35.49771509918959	37.0	35.0	37.0	33.0	37.0
92-93	35.4362075803013	37.0	33.0	37.0	33.0	37.0
94-95	35.39122801300867	37.0	33.0	37.0	33.0	37.0
96-97	35.349199835275996	37.0	33.0	37.0	33.0	37.0
98-99	35.2972619232937	37.0	33.0	37.0	33.0	37.0
100	35.195150062873715	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	1.0
18	6.0
19	4.0
20	24.0
21	88.0
22	360.0
23	1006.0
24	2483.0
25	5051.0
26	9155.0
27	15357.0
28	23095.0
29	32749.0
30	44733.0
31	61015.0
32	81423.0
33	111929.0
34	162616.0
35	252049.0
36	506069.0
37	1647152.0
38	2806590.0
39	143380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.74151367246362	0.0	24.49796184564206	29.760524481894322
2	14.977034676216702	23.837782596740833	38.50860166845685	22.67658105858562
3	16.71392840912708	24.186842926891376	33.002145572487905	26.097083091493634
4	13.068153916544716	23.37699217304046	36.84965132420939	26.705202586205434
5	17.513403049791705	35.782354619343224	32.20197737841172	14.502264952453354
6	28.129414220692112	37.12551451093969	20.050870785056794	14.694200483311398
7	26.241712926302714	32.76360627576788	22.14899014397587	18.845690653953543
8	24.21439887361659	32.11137461340252	21.828690100141593	21.845536412839294
9	23.822887180328518	20.481002015293065	23.503941613897073	32.19216919048134
10-11	24.6511755086105	26.022939429294333	28.265242907744682	21.06064215435049
12-13	25.116049084229363	24.100538116941177	28.25184204694043	22.531570751889028
14-15	22.5716378865615	26.36008578582631	26.38861446612342	24.679661861488768
16-17	21.677955531317934	28.872576088409456	27.257463234612523	22.19200514566009
18-19	21.70221098156285	27.682204331077674	29.272420329625675	21.343164357733794
20-21	22.471241320628323	26.94914905564491	29.215972067193757	21.363637556533007
22-23	22.312712035671986	26.795458657430615	29.029859888715958	21.86196941818144
24-25	22.050096339113683	27.072188697622224	29.279527409007176	21.598187554256917
26-27	22.040936343677046	27.347118922319506	29.06731976937071	21.54462496463274
28-29	21.767359967585666	27.050867461788364	29.328396866546573	21.853375704079397
30-31	22.169511158933325	27.12211663685091	29.135391264359228	21.57298093985654
32-33	21.94490084184586	27.256104796688245	29.009719953231254	21.789274408234633
34-35	21.955643469522666	27.18423627071953	29.19590783905604	21.664212420701762
36-37	22.137515809148	27.213599621325262	28.970997292379753	21.677887277146983
38-39	21.83902278693275	27.320841014912585	29.081643586714357	21.75849261144031
40-41	22.149475281863612	27.022209209573344	28.83137044142949	21.996945067133552
42-43	22.140207382207027	27.089773542255063	28.775052438122145	21.994966637415768
44-45	22.094634616200775	27.50296840864564	28.44326396399792	21.959133011155664
46-47	22.338953259948642	27.17170160747128	28.01536034620302	22.473984786377056
48-49	22.17376298567215	27.385825525625133	28.111097009032708	22.329314479670003
50-51	22.182114231846537	27.743796993329035	27.788928631031045	22.285160143793384
52-53	22.38421716193212	27.568879976007537	27.8331713575737	22.21373150448664
54-55	22.2063572975857	27.63766167565208	27.867633936946838	22.28834708981538
56-57	22.39537128354542	28.06084153968356	27.33372251044518	22.210064666325838
58-59	22.282993301107673	27.88153812077364	27.747355528959357	22.088113049159336
60-61	22.43440158227943	28.126417846765882	27.451729359906974	21.987451211047713
62-63	22.202920570000792	28.355307214297543	27.376601331810846	22.06517088389082
64-65	22.32195393588091	28.03590035727105	27.328299833077185	22.31384587377086
66-67	22.309026863825114	27.981909122337235	27.355632935852668	22.353431077984986
68-69	22.396398035505054	27.904907684432988	27.437131749925527	22.261562530136427
70-71	22.456956842374673	27.455751063347307	27.52763461239206	22.559657481885967
72-73	22.41252921725306	27.574056650396688	27.481808388118477	22.531605744231776
74-75	22.304818505143153	27.775410829871777	27.474898174703625	22.44487249028144
76-77	22.551141681030472	27.512684529504227	27.33254614509019	22.60362764437511
78-79	22.3693313803978	27.632655400207582	27.46015789849482	22.537855320899798
80-81	22.45999387921128	27.703221995973315	27.32729994515633	22.509484179659072
82-83	22.587683451636465	27.503303330146426	27.426063277459832	22.482949940757273
84-85	22.591117716833395	27.513966304081546	27.479702978215425	22.41521300086963
86-87	22.45421562724365	27.598763034084584	27.47293007762657	22.474091261045192
88-89	22.59542694757821	27.408133549018153	27.40073330119041	22.595706202213222
90-91	22.5843423666878	27.471242074015155	27.46276668280251	22.481648876494535
92-93	22.552454954442243	27.476011425262197	27.516503297030315	22.45503032326524
94-95	22.545485828114305	27.3886453305555	27.521720225355907	22.54414861597429
96-97	22.609933960237644	27.65200637549884	27.625251968652808	22.112807695610705
98-99	23.652929593548347	29.759548003299592	27.149150169671326	19.43837223348074
100	22.79951625734596	29.97655067108066	21.504093207072653	25.71983986450073
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1032.0
1	976.0
2	1505.5
3	2223.5
4	3155.5
5	5271.0
6	6933.5
7	8178.5
8	9238.5
9	9434.0
10	8933.0
11	8259.0
12	8926.0
13	11487.0
14	18113.5
15	28149.0
16	37114.5
17	42758.5
18	43856.5
19	40914.0
20	36058.5
21	30997.0
22	27670.5
23	27675.0
24	30791.0
25	36982.0
26	45586.5
27	55366.0
28	65988.0
29	78303.0
30	91195.0
31	104053.0
32	117173.5
33	130285.0
34	144049.5
35	156007.5
36	168376.5
37	181454.0
38	192031.5
39	201044.5
40	209749.5
41	217546.5
42	222332.5
43	227079.0
44	233235.5
45	240897.5
46	249446.5
47	255272.5
48	254675.0
49	249023.0
50	243127.0
51	234695.5
52	221767.0
53	208625.5
54	196652.5
55	183879.0
56	170161.5
57	154609.5
58	138157.5
59	120899.5
60	101896.5
61	83855.5
62	67612.5
63	52518.0
64	39693.5
65	29586.0
66	21975.0
67	16615.0
68	12595.0
69	9072.0
70	6347.0
71	4598.0
72	3255.0
73	2221.0
74	1542.0
75	1052.5
76	701.5
77	416.5
78	244.5
79	175.0
80	126.0
81	87.5
82	61.5
83	40.5
84	23.5
85	16.0
86	13.0
87	10.5
88	9.0
89	7.0
90	6.5
91	5.0
92	3.0
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.2010257795990984E-4
2	6.941696689504849E-4
3	0.0037417438253184677
4	0.014408253372606405
5	5.417909611320857E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	8.465483767688839E-6
18-19	1.6930967535377678E-5
20-21	5.079428290028244E-5
22-23	4.995150470864896E-4
24-25	0.012634707405750756
26-27	0.055810539652676815
28-29	0.0858317149119
30-31	0.07178649157970507
32-33	0.032458774719946334
34-35	0.0034024003550775705
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	9.085299331540017E-6
54-55	2.5624604305775475E-4
56-57	5.997240531233721E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	3.904313094675688E-5
70-71	0.0010742010801929542
72-73	0.014525723206528044
74-75	0.024281095046295553
76-77	0.0029349175951042337
78-79	6.12888242995479E-5
80-81	7.117145533661108E-4
82-83	0.015458122394371493
84-85	0.048793001659424844
86-87	0.037537035018345655
88-89	0.008817224625509596
90-91	9.779287989600054E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	97.0
20-21	347.0
22-23	856.0
24-25	2305.0
26-27	2887.0
28-29	5535.0
30-31	11165.0
32-33	14892.0
34-35	21211.0
36-37	29023.0
38-39	33577.0
40-41	36400.0
42-43	44742.0
44-45	53344.0
46-47	52039.0
48-49	44968.0
50-51	39975.0
52-53	38980.0
54-55	42920.0
56-57	46648.0
58-59	47152.0
60-61	47793.0
62-63	47905.0
64-65	50807.0
66-67	55126.0
68-69	50490.0
70-71	45315.0
72-73	43366.0
74-75	44873.0
76-77	45069.0
78-79	47061.0
80-81	47695.0
82-83	46370.0
84-85	47298.0
86-87	49405.0
88-89	53197.0
90-91	54940.0
92-93	57512.0
94-95	75373.0
96-97	273746.0
98-99	1036573.0
100-101	3117360.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.43483800956722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.52259437826142	78.24305781290785
2	6.813904081631588	11.779173910171101
3	1.5529828461719146	4.026954622215182
4	0.5288463364075202	1.8284298967734838
5	0.22303602362328734	0.9639041286088423
6	0.11465943791109884	0.5946341965268314
7	0.06998085117608353	0.4234148474623488
8	0.04161649313251069	0.28776918739478624
9	0.02771574817978846	0.2156045583810576
>10	0.10191565895097367	1.3832232495269807
>50	0.0021017156531473466	0.12068273465726156
>100	6.160998181400394E-4	0.1152460831876074
>500	3.0329082573074806E-5	0.017904772186616538
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.772387014151071E-5	0.0	0.0	0.0
3	0.0	6.772387014151071E-5	0.0	0.0	0.0
4	0.0	6.772387014151071E-5	0.0	0.0	0.0
5	0.0	6.772387014151071E-5	0.0	1.6930967535377678E-5	0.0
6	0.0	6.772387014151071E-5	0.0	1.6930967535377678E-5	0.0
7	0.0	1.0158580521226608E-4	0.0	1.6930967535377678E-5	0.0
8	0.0	1.1851677274764376E-4	0.0	1.6930967535377678E-5	0.0
9	0.0	2.0317161042453217E-4	0.0	1.6930967535377678E-5	0.0
10-11	0.0	2.0317161042453217E-4	0.0	1.6930967535377678E-5	0.0
12-13	0.0	2.11637094192221E-4	0.0	4.2327418838444196E-5	0.0
14-15	0.0	3.047574156367982E-4	0.0	8.46548376768884E-5	1.6930967535377678E-5
16-17	0.0	3.555503182429313E-4	0.0	1.0158580521226608E-4	1.6930967535377678E-5
18-19	0.0	3.555503182429313E-4	0.0	1.1005128897995492E-4	3.3861935070755357E-5
20-21	0.0	3.555503182429313E-4	0.0	1.1851677274764376E-4	3.3861935070755357E-5
22-23	0.0	4.0634322084906433E-4	0.0	1.5237870781839913E-4	4.2327418838444196E-5
24-25	0.0	4.5713612345519737E-4	0.0	2.0317161042453217E-4	5.079290260613304E-5
26-27	0.0	4.7406709099057505E-4	0.0	2.539645130306652E-4	5.079290260613304E-5
28-29	0.0	4.7406709099057505E-4	0.0	4.5713612345519737E-4	5.079290260613304E-5
30-31	0.0	5.587219286674634E-4	0.0	0.0011343748248703045	5.9258386373821874E-5
32-33	0.0	5.587219286674634E-4	0.0	0.002167163844528343	6.772387014151071E-5
34-35	0.0	6.518422501120407E-4	0.0	0.0037671402766215334	6.772387014151071E-5
36-37	0.0	7.44962571556618E-4	0.0	0.0065099570173527185	8.46548376768884E-5
38-39	0.0	7.618935390919956E-4	0.0	0.010090856651085098	8.46548376768884E-5
40-41	0.0	9.312032144457724E-4	0.0	0.013764876606262053	8.46548376768884E-5
42-43	0.0	0.0010412545034257275	0.0	0.017379638175065187	1.0158580521226608E-4
44-45	0.0	0.0011767022437087489	0.0	0.022221894890183204	1.1851677274764376E-4
46-47	0.0	0.0011851677274764376	0.0	0.02720806482935193	1.439132240507103E-4
48-49	0.0	0.0011936332112441263	0.0	0.03209264896330839	1.5237870781839913E-4
50-51	0.0	0.001269822565153326	0.0	0.03677406148684032	1.5237870781839913E-4
52-53	0.0	0.001269822565153326	0.0	0.04139621562399842	1.693096753537768E-4
54-55	0.0	0.0012867535326887038	0.0	0.04645010943330867	1.862406428891545E-4
56-57	0.0	0.0013036845002240814	0.0	0.05224896581417552	1.862406428891545E-4
58-59	0.0	0.0013290809515271477	0.0	0.05750603123391029	1.9470612665684333E-4
60-61	0.0	0.0013883393379009697	0.0	0.06267844181596817	2.0317161042453217E-4
62-63	0.0	0.001430666756739414	0.0	0.06819793723250128	2.0317161042453217E-4
64-65	0.0	0.0015745799807901242	0.0	0.07377669103540824	2.0317161042453217E-4
66-67	0.0	0.0015745799807901242	0.0	0.07899989452007225	2.11637094192221E-4
68-69	0.0	0.0016084419158608797	0.0	0.08469716509572683	2.2010257795990984E-4
70-71	0.0	0.0016592348184670128	0.0	0.08961561116475406	2.2010257795990984E-4
72-73	0.0	0.0017015622373054567	0.0	0.09323037273355719	2.539645130306652E-4
74-75	0.0	0.0017523551399115898	0.0	0.09601551689312682	2.539645130306652E-4
76-77	0.0	0.001786217074982345	0.0	0.0984620417019889	2.539645130306652E-4
78-79	0.0	0.001803148042517723	0.0	0.10012127652045591	2.539645130306652E-4
80-81	0.0	0.0018200790100531008	0.0	0.10069692941665875	2.6242999679835404E-4
82-83	0.0	0.0018285444938207895	0.0	0.10109480715374013	2.7089548056604285E-4
84-85	0.0	0.0018285444938207895	0.0	0.10128951328039697	2.7089548056604285E-4
86-87	0.0	0.0018285444938207895	0.0	0.10138263360184155	2.7089548056604285E-4
88	0.0	0.0019470612665684333	0.0	0.10139956456937693	2.7089548056604285E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9035	0.0	51.815723	1
GTATCAA	16765	0.0	37.73999	1
CTTATAC	4760	0.0	36.89313	1
ATCAACG	19760	0.0	31.373692	3
TCAACGC	20595	0.0	30.080273	4
TATCAAC	21160	0.0	29.64004	2
CAACGCA	21180	0.0	29.226152	5
AACGCAG	21370	0.0	29.006094	6
ACGCAGA	21525	0.0	28.75772	7
TATACAC	7030	0.0	27.822592	3
CGCAGAG	25085	0.0	24.693445	8
TTATACA	7255	0.0	24.439993	2
ACACATC	8080	0.0	22.204311	6
GTGGTAT	4355	0.0	21.968315	1
GCAGAGT	28785	0.0	21.5046	9
GAGTACT	17795	0.0	20.785358	12-13
TGGTATC	4350	0.0	20.62508	2
GTACATG	18635	0.0	20.125263	1
ACATCTC	8645	0.0	20.064642	8
TACACAT	9805	0.0	19.64205	5
>>END_MODULE