FastQCFastQC Report
Fri 27 May 2016
SRR2138771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6173769
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99600.050.826141
GTATCAA181850.038.6242071
TCAACGC221450.031.1369234
ATCAACG222300.030.9795863
TATCAAC229500.030.3995692
CTTATAC44250.030.3910271
CAACGCA228200.030.2665795
AACGCAG233450.029.677396
ACGCAGA266700.025.9453437
CGCAGAG269750.025.6202378
GTGGTAT46300.024.3278541
TATACAC67100.023.61613
TGGTATC46300.022.8478322
GCAGAGT304750.022.7340019
TTATACA68250.021.7748832
GTACATG199200.021.134561
GAGTACT189300.020.99125712-13
TACATGG198750.020.7514362
CAGAGTA297400.019.9051410-11
ACATGGG202450.019.8644623
Produced by FastQC (version 0.11.5)