##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138771_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6173769
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.838758625403706	33.0	33.0	33.0	33.0	33.0
2	32.83537576478809	33.0	33.0	33.0	33.0	33.0
3	32.83400383137108	33.0	33.0	33.0	33.0	33.0
4	36.78920866653741	37.0	37.0	37.0	37.0	37.0
5	36.8006023548986	37.0	37.0	37.0	37.0	37.0
6	36.81120333462428	37.0	37.0	37.0	37.0	37.0
7	36.8000971529709	37.0	37.0	37.0	37.0	37.0
8	36.79482679057153	37.0	37.0	37.0	37.0	37.0
9	36.78985154773365	37.0	37.0	37.0	37.0	37.0
10-11	36.769196984856414	37.0	37.0	37.0	37.0	37.0
12-13	36.75635320984637	37.0	37.0	37.0	37.0	37.0
14-15	39.108112807589656	40.0	40.0	40.0	37.0	40.0
16-17	39.06705118056733	40.0	40.0	40.0	37.0	40.0
18-19	39.09740808248576	40.0	40.0	40.0	37.0	40.0
20-21	39.13669787013477	40.0	40.0	40.0	37.0	40.0
22-23	39.15565035330832	40.0	40.0	40.0	37.0	40.0
24-25	39.067516933523336	40.0	40.0	40.0	37.0	40.0
26-27	38.99052608238826	40.0	40.0	40.0	37.0	40.0
28-29	38.963346497153196	40.0	40.0	40.0	37.0	40.0
30-31	38.91731307091757	40.0	40.0	40.0	37.0	40.0
32-33	38.76354609750156	40.0	38.5	40.0	37.0	40.0
34-35	38.83294365329688	40.0	37.0	40.0	37.0	40.0
36-37	38.52471066025313	40.0	37.0	40.0	37.0	40.0
38-39	38.69880547570719	40.0	37.0	40.0	37.0	40.0
40-41	38.7051043590911	40.0	37.0	40.0	37.0	40.0
42-43	38.652665436546826	40.0	37.0	40.0	37.0	40.0
44-45	38.5683276634798	40.0	37.0	40.0	37.0	40.0
46-47	38.486973671930144	40.0	37.0	40.0	37.0	40.0
48-49	38.43655167027363	40.0	37.0	40.0	37.0	40.0
50-51	38.37563997361177	40.0	37.0	40.0	37.0	40.0
52-53	38.32079302180261	40.0	37.0	40.0	37.0	40.0
54-55	38.2336572242983	40.0	37.0	40.0	37.0	40.0
56-57	38.113072839445834	40.0	37.0	40.0	37.0	40.0
58-59	37.98508813638033	40.0	37.0	40.0	37.0	40.0
60-61	37.82347004806504	40.0	37.0	40.0	33.0	40.0
62-63	37.63247855555899	38.5	37.0	40.0	33.0	40.0
64-65	37.413151872241045	37.0	37.0	40.0	33.0	40.0
66-67	37.171659800796306	37.0	37.0	40.0	33.0	40.0
68-69	36.91492569615065	37.0	37.0	40.0	33.0	40.0
70-71	36.660716929920824	37.0	37.0	40.0	33.0	40.0
72-73	36.40840798528518	37.0	37.0	37.0	33.0	40.0
74-75	36.1598231079962	37.0	37.0	37.0	33.0	40.0
76-77	34.482908395006376	35.0	33.0	37.0	30.0	37.0
78-79	35.771219869413926	37.0	35.0	37.0	33.0	37.0
80-81	35.88965350852249	37.0	37.0	37.0	33.0	37.0
82-83	35.81470659157062	37.0	37.0	37.0	33.0	37.0
84-85	35.704640368200806	37.0	37.0	37.0	33.0	37.0
86-87	35.62253109122959	37.0	37.0	37.0	33.0	37.0
88-89	35.54664301976783	37.0	37.0	37.0	33.0	37.0
90-91	35.47649264834449	37.0	33.0	37.0	33.0	37.0
92-93	35.412675549749245	37.0	33.0	37.0	33.0	37.0
94-95	35.3654200169741	37.0	33.0	37.0	33.0	37.0
96-97	35.32339986927058	37.0	33.0	37.0	33.0	37.0
98-99	35.27887977292912	37.0	33.0	37.0	33.0	37.0
100	35.188895982976774	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	22.0
21	78.0
22	312.0
23	1079.0
24	2541.0
25	5355.0
26	9632.0
27	16153.0
28	24178.0
29	34631.0
30	47600.0
31	64990.0
32	87019.0
33	119761.0
34	173063.0
35	270880.0
36	549302.0
37	1772088.0
38	2879772.0
39	115310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18998151696314	19.82510197579469	14.459449325039534	23.52546718220264
2	14.762586679223016	20.68323256020755	41.63195934282607	22.92222141774336
3	18.347794353585346	25.780019608913456	30.34520661195846	25.52697942554274
4	12.593496352489977	18.03191443689473	38.25755151786931	31.117037692745985
5	13.600688984476792	37.400457163293986	35.07385493747372	13.924998914755502
6	30.131140313153924	37.308733125583416	18.615403329797406	13.944723231465254
7	26.681076016935524	32.73873706645001	22.36564082653562	18.214546090078848
8	24.695659976911998	36.16095775530312	20.866297394670905	18.277084873113978
9	25.620718883391973	16.978947544036714	20.931816528930707	36.4685170436406
10-11	24.307266760385755	26.679318581566626	28.379965301584818	20.6334493564628
12-13	25.351312949998615	24.279730906679532	28.522843987198094	21.846112156123755
14-15	22.444458158379426	25.644318729774305	26.419347727457893	25.49187538438837
16-17	21.83976271221032	28.84122324628602	27.043164394391823	22.275849647111837
18-19	21.811174017038862	27.686474825993653	29.090892451596424	21.41145870537106
20-21	22.582361615146713	26.716419566500722	29.119587161131022	21.581631657221543
22-23	22.3294506213492	26.79843151535435	28.99119333112973	21.880924532166723
24-25	22.21332734664367	26.96453040309612	29.01252756941075	21.80961468084946
26-27	22.040558324594866	27.092012510095554	29.013867142383056	21.853562022926525
28-29	21.8405886567545	27.026230211417158	29.207351408972986	21.925829722855358
30-31	22.187743708923584	26.878034520047805	28.90972978825769	22.024491982770925
32-33	22.07113458312077	27.046966156066937	29.000705076854356	21.881194183957945
34-35	22.16775132832936	27.2652045463329	28.902984136364545	21.664059988973193
36-37	22.14600532770823	27.20222974516251	28.668450207467348	21.9833147196619
38-39	21.971516179569686	27.16645874646284	28.8854339250923	21.976591148875173
40-41	22.198197285926774	27.034795867876415	28.80012402956093	21.96688281663588
42-43	22.187967629243115	27.129812483301375	28.59255613132838	22.08966375612713
44-45	22.19601841451184	27.320843635030478	28.338679430489368	22.144458519968317
46-47	22.432333153903876	27.29976799551206	27.883125515393743	22.38477333519032
48-49	22.285394838490333	27.472245153205797	27.839517890112862	22.402842118191003
50-51	22.294720064574545	27.61926063971484	27.762799821923913	22.3232194737867
52-53	22.414390933586255	27.56253922307012	27.76565791897524	22.25741192436838
54-55	22.339817061044894	27.560213934455653	27.641383892767617	22.458585111731836
56-57	22.49298598393771	27.729102888048672	27.441562774794658	22.336348353218956
58-59	22.33027843357665	27.80953199001983	27.638380463596313	22.221809112807207
60-61	22.52263867964016	27.895642053050825	27.43041345083752	22.1513058164715
62-63	22.35252586553683	28.016334827516925	27.38607846227587	22.245060844670373
64-65	22.352525847158063	27.95990562818853	27.350137436001955	22.337431088651453
66-67	22.447046492250312	27.830974578201534	27.27013802042385	22.451840909124307
68-69	22.429339639622683	27.737339252728454	27.382921142295004	22.450399965353864
70-71	22.45881432109838	27.560163788195318	27.441475740773253	22.539546149933052
72-73	22.50043583947402	27.571691228002614	27.349245556443126	22.57862737608024
74-75	22.377707643981967	27.723401717750257	27.317317585052507	22.58157305321527
76-77	22.511148273355232	27.67988752156357	27.241998987502132	22.566965217579074
78-79	22.48555995505885	27.678167782741337	27.20589075227247	22.630381509927343
80-81	22.476972693590504	27.70421473040175	27.239945773729808	22.578866802277943
82-83	22.51265283593081	27.682395792327284	27.254767370394923	22.550184001346977
84-85	22.609130323084305	27.646043148274646	27.237970720671385	22.506855807969664
86-87	22.494526637699845	27.671983168625786	27.322602511540918	22.510887682133447
88-89	22.503124829400246	27.641584350911184	27.279826620928517	22.575464198760052
90-91	22.569452207220834	27.66571462267779	27.250985801383454	22.513847368717922
92-93	22.591493537896017	27.62677923186943	27.28063749981462	22.50108973041993
94-95	22.5030656068201	27.664684650300387	27.28965297603715	22.54259676684236
96-97	22.564547288261583	27.80147901258061	27.32211499802819	22.31185870112962
98-99	23.656554973762738	29.728074570901008	27.53899327759255	19.076377177743705
100	22.579473280916684	30.64720993396044	21.417452004784394	25.35586478033848
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1897.0
1	1619.5
2	1891.0
3	2614.5
4	3517.5
5	5434.5
6	7051.0
7	8496.5
8	9873.0
9	10380.0
10	9970.0
11	9311.5
12	10020.0
13	12918.0
14	19825.0
15	29259.5
16	37104.5
17	41882.0
18	42949.0
19	40473.0
20	36178.5
21	31545.5
22	28064.0
23	27769.0
24	30997.0
25	37369.5
26	46117.5
27	56222.0
28	67635.0
29	80581.0
30	93611.0
31	107042.5
32	120982.0
33	134127.0
34	147267.5
35	159777.0
36	173046.0
37	186175.5
38	196947.5
39	206012.0
40	215157.0
41	223283.0
42	228132.5
43	233369.0
44	239062.0
45	244368.5
46	249428.0
47	253388.0
48	258645.5
49	257971.0
50	251698.0
51	245660.5
52	236442.0
53	224240.5
54	212363.0
55	199517.0
56	184067.0
57	167232.5
58	149619.5
59	131020.5
60	110739.5
61	91351.5
62	73481.0
63	57122.0
64	42969.0
65	32007.0
66	23772.5
67	17841.5
68	13596.5
69	9841.0
70	6952.5
71	4890.5
72	3432.0
73	2430.5
74	1630.0
75	1032.5
76	638.5
77	404.0
78	257.5
79	168.0
80	110.5
81	62.5
82	40.0
83	33.5
84	27.0
85	20.5
86	15.5
87	11.0
88	7.5
89	4.5
90	2.0
91	1.0
92	1.0
93	1.0
94	1.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	7.450878061683228E-4
4	0.01946946832639835
5	7.288902451646636E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	8.09894448076371E-6
22-23	0.0
24-25	0.007299188469582961
26-27	0.049669647007567874
28-29	0.08476340154054685
30-31	0.0753956587879326
32-33	0.03463577059425516
34-35	0.004280410420426804
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.9694107894239716E-4
56-57	7.305067358001813E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.743215422047539E-5
72-73	0.011929298896039652
74-75	0.027262823899476398
76-77	0.004040231105057909
78-79	0.0
80-81	0.0
82-83	0.010128936329525958
84-85	0.04612223846331928
86-87	0.03768252567555295
88-89	0.00992297418119229
90-91	1.6408630693615382E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	67.0
20-21	328.0
22-23	783.0
24-25	2246.0
26-27	2759.0
28-29	4896.0
30-31	11097.0
32-33	14505.0
34-35	20551.0
36-37	29137.0
38-39	34613.0
40-41	37011.0
42-43	45235.0
44-45	54754.0
46-47	53437.0
48-49	46403.0
50-51	41185.0
52-53	39363.0
54-55	42758.0
56-57	46134.0
58-59	46687.0
60-61	46205.0
62-63	46461.0
64-65	49394.0
66-67	53369.0
68-69	49724.0
70-71	45876.0
72-73	43477.0
74-75	44293.0
76-77	44330.0
78-79	47525.0
80-81	47824.0
82-83	46188.0
84-85	46179.0
86-87	48359.0
88-89	51478.0
90-91	54294.0
92-93	55862.0
94-95	67554.0
96-97	197816.0
98-99	381846.0
100-101	4131766.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.63154891056615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.37926850772234	80.07706837646228
2	6.286619258236362	11.018123660204935
3	1.352058317446659	3.5544889372579407
4	0.4662917169995036	1.6344746161933548
5	0.20426310120360386	0.8949945971873766
6	0.10049175698577893	0.5283748990444809
7	0.0525542341016917	0.3223786255297818
8	0.03976134097793398	0.27874783173260154
9	0.023153434539395133	0.18260741981579365
>10	0.09293509769302583	1.290253648241173
>50	0.001965493907302812	0.11132415217077928
>100	5.821319221902993E-4	0.07826611958182443
>500	5.560826423846181E-5	0.028897116577716028
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.619756100365919E-5
2	0.0	4.8592683010977574E-5	0.0	0.0	3.239512200731838E-5
3	0.0	4.8592683010977574E-5	0.0	0.0	3.239512200731838E-5
4	0.0	8.098780501829595E-5	0.0	1.619756100365919E-5	4.8592683010977574E-5
5	0.0	8.098780501829595E-5	0.0	1.619756100365919E-5	4.8592683010977574E-5
6	4.8592683010977574E-5	8.098780501829595E-5	0.0	1.619756100365919E-5	4.8592683010977574E-5
7	4.8592683010977574E-5	8.098780501829595E-5	0.0	1.619756100365919E-5	6.479024401463677E-5
8	4.8592683010977574E-5	9.718536602195515E-5	0.0	1.619756100365919E-5	9.718536602195515E-5
9	4.8592683010977574E-5	1.943707320439103E-4	0.0	1.619756100365919E-5	9.718536602195515E-5
10-11	6.479024401463677E-5	1.943707320439103E-4	0.0	2.4296341505488787E-5	9.718536602195515E-5
12-13	7.288902451646636E-5	1.943707320439103E-4	0.0	3.239512200731838E-5	9.718536602195515E-5
14-15	9.718536602195515E-5	2.5106219555671744E-4	0.0	3.239512200731838E-5	1.3767926853110312E-4
16-17	1.1338292702561433E-4	2.5916097605854706E-4	0.0	3.239512200731838E-5	1.538768295347623E-4
18-19	1.1338292702561433E-4	2.9155609806586543E-4	0.0	3.239512200731838E-5	1.862719515420807E-4
20-21	1.2958048802927353E-4	3.077536590695246E-4	0.0	3.239512200731838E-5	2.2676585405122867E-4
22-23	1.619756100365919E-4	3.644451225823318E-4	0.0	5.669146351280717E-5	2.2676585405122867E-4
24-25	1.619756100365919E-4	4.130378055933094E-4	0.0	6.479024401463677E-5	2.2676585405122867E-4
26-27	1.619756100365919E-4	4.3733414709879815E-4	0.0	8.098780501829595E-5	2.2676585405122867E-4
28-29	1.619756100365919E-4	4.4543292760062777E-4	0.0	3.0775365906952467E-4	2.2676585405122867E-4
30-31	1.619756100365919E-4	4.8592683010977575E-4	0.0	8.422731721902779E-4	2.2676585405122867E-4
32-33	1.862719515420807E-4	4.8592683010977575E-4	0.0	0.0021704731744903315	2.2676585405122867E-4
34-35	2.024695125457399E-4	5.912109766335605E-4	0.0	0.003676846347830636	2.2676585405122867E-4
36-37	2.1056829304756948E-4	7.126926841610043E-4	0.0	0.006227962205906959	2.3486463455305826E-4
38-39	2.1056829304756948E-4	7.450878061683228E-4	0.0	0.010253056115316267	2.4296341505488788E-4
40-41	2.1056829304756948E-4	8.017792696811299E-4	0.0	0.014043285390172518	2.4296341505488788E-4
42-43	2.1056829304756948E-4	8.179768306847892E-4	0.0	0.017671539054992177	2.5106219555671744E-4
44-45	2.1056829304756948E-4	8.503719526921075E-4	0.0	0.02219065857501309	2.9155609806586543E-4
46-47	2.1056829304756948E-4	8.746682941975963E-4	0.0	0.026928445168583406	3.077536590695246E-4
48-49	2.1866707354939907E-4	8.746682941975963E-4	0.0	0.03165813298165189	3.239512200731838E-4
50-51	2.5106219555671744E-4	9.39458538212233E-4	0.0	0.036695574453789896	3.320500005750134E-4
52-53	2.5916097605854706E-4	9.556560992158923E-4	0.0	0.04123089153481447	3.482475615786726E-4
54-55	2.672597565603767E-4	9.880512212232105E-4	0.0	0.04620354276293784	3.8064268358599103E-4
56-57	2.7535853706220625E-4	0.0010285451237323586	0.0	0.05137866350360695	4.049390250914798E-4
58-59	2.7535853706220625E-4	0.0010528414652378475	0.0	0.056902031805854736	4.049390250914798E-4
60-61	2.834573175640358E-4	0.0010852365872451657	0.0	0.06221483181505495	4.049390250914798E-4
62-63	3.239512200731838E-4	0.0011419280507579729	0.0	0.06717128548217466	4.2113658609513896E-4
64-65	3.40148781076843E-4	0.0012310146362780985	0.0	0.0722978135398328	4.2113658609513896E-4
66-67	3.482475615786726E-4	0.0012310146362780985	0.0	0.0776025147685312	4.4543292760062777E-4
68-69	3.725439030841614E-4	0.0012310146362780985	0.0	0.0827776355092003	4.940256106116053E-4
70-71	3.8064268358599103E-4	0.0012310146362780985	0.0	0.08717527332169377	5.183219521170941E-4
72-73	4.049390250914798E-4	0.0012310146362780985	0.0	0.09050387210794572	5.993097571353901E-4
74-75	4.049390250914798E-4	0.001239113416779928	0.0	0.09337893918609524	5.993097571353901E-4
76-77	4.2113658609513896E-4	0.001279607319289076	0.0	0.09627830260575024	5.993097571353901E-4
78-79	4.2113658609513896E-4	0.0012958048802927352	0.0	0.09780897212059603	5.993097571353901E-4
80-81	4.2113658609513896E-4	0.0013039036607945648	0.0	0.09828680017020397	6.479024401463676E-4
82-83	4.2113658609513896E-4	0.0013120024412963944	0.0	0.09871603553680094	6.80297562153686E-4
84-85	4.2113658609513896E-4	0.0013120024412963944	0.0	0.0989266038298485	6.80297562153686E-4
86-87	4.2113658609513896E-4	0.0013282000023000537	0.0	0.09899139407386315	6.80297562153686E-4
88	4.2113658609513896E-4	0.0013767926853110312	0.0	0.09899949285436498	6.964951231573452E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9960	0.0	50.82614	1
GTATCAA	18185	0.0	38.624207	1
TCAACGC	22145	0.0	31.136923	4
ATCAACG	22230	0.0	30.979586	3
TATCAAC	22950	0.0	30.399569	2
CTTATAC	4425	0.0	30.391027	1
CAACGCA	22820	0.0	30.266579	5
AACGCAG	23345	0.0	29.67739	6
ACGCAGA	26670	0.0	25.945343	7
CGCAGAG	26975	0.0	25.620237	8
GTGGTAT	4630	0.0	24.327854	1
TATACAC	6710	0.0	23.6161	3
TGGTATC	4630	0.0	22.847832	2
GCAGAGT	30475	0.0	22.734001	9
TTATACA	6825	0.0	21.774883	2
GTACATG	19920	0.0	21.13456	1
GAGTACT	18930	0.0	20.991257	12-13
TACATGG	19875	0.0	20.751436	2
CAGAGTA	29740	0.0	19.90514	10-11
ACATGGG	20245	0.0	19.864462	3
>>END_MODULE