FastQCFastQC Report
Fri 27 May 2016
SRR2138770_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138770_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4941006
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69900.053.1573031
GTATCAA134100.039.9597631
TCAACGC165900.031.7357854
CTTATAC39300.031.6239571
ATCAACG168500.031.271353
TATCAAC171050.031.0042252
CAACGCA170800.030.9676385
AACGCAG175050.030.2643996
ACGCAGA198300.026.6301677
CGCAGAG200850.026.2920688
TATACAC59700.024.382473
TGGTATC30700.023.2914662
TTATACA58650.022.932162
GTGGTAT33700.022.7336161
GCAGAGT232250.022.6457969
CTCGTAT58400.022.5877889
TCTCGTA58900.021.6735868
GAGTACT141050.021.14802212-13
GTACATG159700.021.0813051
AATCTCG60800.021.0662736
Produced by FastQC (version 0.11.5)