##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138770_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4941006
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8340119400786	33.0	33.0	33.0	33.0	33.0
2	32.833623152855914	33.0	33.0	33.0	33.0	33.0
3	32.833784051264054	33.0	33.0	33.0	33.0	33.0
4	36.78634533129488	37.0	37.0	37.0	37.0	37.0
5	36.79516985002649	37.0	37.0	37.0	37.0	37.0
6	36.80848009494423	37.0	37.0	37.0	37.0	37.0
7	36.798568348227064	37.0	37.0	37.0	37.0	37.0
8	36.777549349262074	37.0	37.0	37.0	37.0	37.0
9	36.773688799406436	37.0	37.0	37.0	37.0	37.0
10-11	36.766144283168245	37.0	37.0	37.0	37.0	37.0
12-13	36.75246285877815	37.0	37.0	37.0	37.0	37.0
14-15	39.10548266891398	40.0	40.0	40.0	37.0	40.0
16-17	39.0691844940079	40.0	40.0	40.0	37.0	40.0
18-19	39.09905290542048	40.0	40.0	40.0	37.0	40.0
20-21	39.130622224862904	40.0	40.0	40.0	37.0	40.0
22-23	39.1575326177238	40.0	40.0	40.0	37.0	40.0
24-25	39.066507565835565	40.0	40.0	40.0	37.0	40.0
26-27	38.98099861146878	40.0	40.0	40.0	37.0	40.0
28-29	38.956929429659894	40.0	40.0	40.0	37.0	40.0
30-31	38.895732569545345	40.0	40.0	40.0	37.0	40.0
32-33	38.74858149987065	40.0	38.5	40.0	37.0	40.0
34-35	38.8268103319793	40.0	38.5	40.0	37.0	40.0
36-37	38.52119621686445	40.0	37.0	40.0	37.0	40.0
38-39	38.69675089797635	40.0	37.0	40.0	37.0	40.0
40-41	38.71073165653942	40.0	37.0	40.0	37.0	40.0
42-43	38.66276960731912	40.0	37.0	40.0	37.0	40.0
44-45	38.58197340557351	40.0	37.0	40.0	37.0	40.0
46-47	38.50323376714602	40.0	37.0	40.0	37.0	40.0
48-49	38.45531058144559	40.0	37.0	40.0	37.0	40.0
50-51	38.39169743188977	40.0	37.0	40.0	37.0	40.0
52-53	38.33097954397524	40.0	37.0	40.0	37.0	40.0
54-55	38.24881055542587	40.0	37.0	40.0	37.0	40.0
56-57	38.12754172664951	40.0	37.0	40.0	37.0	40.0
58-59	37.99734261081822	40.0	37.0	40.0	37.0	40.0
60-61	37.83716124650512	40.0	37.0	40.0	33.0	40.0
62-63	37.64669782431979	38.5	37.0	40.0	33.0	40.0
64-65	37.424268928905434	37.0	37.0	40.0	33.0	40.0
66-67	37.188170995088576	37.0	37.0	40.0	33.0	40.0
68-69	36.93465093626805	37.0	37.0	40.0	33.0	40.0
70-71	36.68197326005964	37.0	37.0	40.0	33.0	40.0
72-73	36.43391042250435	37.0	37.0	37.0	33.0	40.0
74-75	36.187634910500385	37.0	37.0	37.0	33.0	40.0
76-77	34.527470971586155	35.0	33.0	37.0	30.0	37.0
78-79	35.79575700793568	37.0	37.0	37.0	33.0	37.0
80-81	35.911090956641594	37.0	37.0	37.0	33.0	37.0
82-83	35.8347353803419	37.0	37.0	37.0	33.0	37.0
84-85	35.72502052655156	37.0	37.0	37.0	33.0	37.0
86-87	35.64481750281475	37.0	37.0	37.0	33.0	37.0
88-89	35.57107516564797	37.0	37.0	37.0	33.0	37.0
90-91	35.5051174479874	37.0	35.0	37.0	33.0	37.0
92-93	35.443100258901744	37.0	33.0	37.0	33.0	37.0
94-95	35.39849206958485	37.0	33.0	37.0	33.0	37.0
96-97	35.357711939768606	37.0	33.0	37.0	33.0	37.0
98-99	35.31367294499121	37.0	33.0	37.0	33.0	37.0
100	35.22029925119397	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	1.0
14	0.0
15	0.0
16	1.0
17	0.0
18	1.0
19	6.0
20	18.0
21	79.0
22	290.0
23	871.0
24	2137.0
25	4452.0
26	7992.0
27	12811.0
28	19371.0
29	27635.0
30	38161.0
31	52105.0
32	69396.0
33	95510.0
34	136486.0
35	215123.0
36	432878.0
37	1402567.0
38	2324478.0
39	98633.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.2572447797068	19.761765114229775	14.42582745295189	23.555162653111534
2	14.693525974265158	20.557716384072393	41.53994146131375	23.2088161803487
3	18.286929892610306	25.994150549577544	30.324549173169597	25.39437038464255
4	12.943687506022176	17.9465255190406	38.02682387882292	31.082963096114312
5	13.615097113502589	37.48012283415547	35.01672039767082	13.888059654671117
6	30.10065156771718	37.43130447524249	18.51981155254618	13.948232404494146
7	26.613487212927893	32.8947789174917	22.300175308429093	18.19155856115131
8	24.62696867803844	36.042255362571915	21.096351633655168	18.234424325734473
9	25.523182930763493	16.958631501358227	20.876598814087657	36.64158675379062
10-11	24.28524676958498	26.55846805286211	28.396757664329897	20.759527513223016
12-13	25.430580331211907	24.133759400413602	28.588034501476017	21.84762576689848
14-15	22.41086126995191	25.66024004018615	26.399451852517487	25.52944683734446
16-17	22.052118536184736	28.718979495268776	26.82523558967546	22.403666378871023
18-19	22.049183911130648	27.549349262073353	28.84362010489362	21.557846721902383
20-21	22.687143556983674	26.555856657854882	29.0545413662376	21.702458418923847
22-23	22.379762915099462	26.690743398616316	29.049387391351605	21.880106294932613
24-25	22.255494709903918	26.92370650406216	28.88295785790462	21.9378409281293
26-27	22.302388519109236	27.056256903713887	28.71409043002786	21.92726414714901
28-29	21.943086129265637	27.034032829738507	29.101250799145618	21.921630241850234
30-31	22.625451925738275	26.961127560870228	28.666623564497307	21.74679694889419
32-33	21.924880190203293	27.474106231268365	28.66293781622228	21.938075762306067
34-35	22.160818916362256	27.464641977829796	28.736635049246118	21.63790405656183
36-37	22.153936857132393	27.463701251647105	28.510809456307157	21.871552434913347
38-39	22.027323006457472	27.203917526410148	28.721088883694446	22.047670583437938
40-41	22.29683217914466	26.98687731608272	28.688948217760448	22.027342287012182
42-43	22.318951517164074	27.00913788386628	28.50008821480498	22.171822384164663
44-45	22.272302178467566	27.27068486692491	28.228411366113725	22.2286015884938
46-47	22.57073302539782	27.13640496827014	27.800468677041707	22.492393329290337
48-49	22.42182607624482	27.32471198628917	27.754988868319973	22.498473069146037
50-51	22.34258288912398	27.538369401694002	27.70400758080756	22.415040128374457
52-53	22.515618119306286	27.457885932765226	27.690945685267792	22.335550262660693
54-55	22.4196849624613	27.40052875735437	27.577549432447352	22.60223684773698
56-57	22.569891290419744	27.610498316607107	27.37328742188041	22.446322971092737
58-59	22.397241100345298	27.75037213044344	27.561762045462867	22.290624723748394
60-61	22.60171865570138	27.804683066557374	27.355956745363326	22.237641532377925
62-63	22.43548872064296	27.993318023309904	27.271095668562868	22.300097587484267
64-65	22.513963573207548	27.838610345927982	27.241908876165045	22.405517204699425
66-67	22.53625070294587	27.755388614876203	27.184139804398978	22.524220877778944
68-69	22.55814369771583	27.607046867228995	27.31487618283031	22.51993325222486
70-71	22.558294001323688	27.41678794946839	27.349942137442973	22.674975911764946
72-73	22.618560038601387	27.438607775394857	27.267533449607935	22.675298736395824
74-75	22.49012261297733	27.528609273769263	27.267818113439823	22.713449999813584
76-77	22.64855719741476	27.431740055298086	27.22245517889374	22.697247568393408
78-79	22.60538974531184	27.458710322315323	27.195745734602205	22.740154197770632
80-81	22.637854799000877	27.47177456606151	27.210479600182097	22.67989103475552
82-83	22.67038073348656	27.405128909763164	27.296542420305787	22.62794793644449
84-85	22.72464682537179	27.4044078212643	27.22692140247711	22.6440239508868
86-87	22.640542018268647	27.4106290536984	27.314289792677503	22.63453913535545
88-89	22.67732471018518	27.338664673327816	27.269851724387074	22.71415889209993
90-91	22.74946083791365	27.30023380194484	27.288611458275785	22.661693901865725
92-93	22.768789379783616	27.236914132274272	27.31680286301799	22.67749362492412
94-95	22.66121567438067	27.270252948425117	27.341208265005623	22.727323112188596
96-97	22.73955665459684	27.390063791194237	27.402047568257938	22.468331985950993
98-99	23.874999320063623	29.314152217834856	27.56151880586412	19.2493296562374
100	22.75774263460386	30.28469792568156	21.42406288294019	25.533496556774388
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	891.0
1	802.0
2	1138.0
3	1663.5
4	2332.5
5	3850.0
6	5136.0
7	6281.5
8	7311.0
9	7550.0
10	7162.5
11	6769.5
12	7491.5
13	9718.0
14	14906.5
15	22184.5
16	28426.0
17	32535.0
18	33542.5
19	31533.0
20	28198.0
21	24511.5
22	21933.0
23	21935.5
24	24498.0
25	29439.5
26	36624.5
27	44821.5
28	53432.5
29	63441.5
30	73930.5
31	84161.0
32	95495.0
33	106464.5
34	116842.5
35	127113.0
36	139530.5
37	153101.0
38	164497.5
39	172664.0
40	179365.0
41	184449.0
42	186327.0
43	188441.5
44	191952.5
45	198604.5
46	205351.5
47	209047.0
48	209394.0
49	206719.0
50	203240.5
51	197106.0
52	188806.0
53	180117.0
54	171112.0
55	161159.0
56	149912.0
57	137140.0
58	122866.5
59	107564.5
60	90753.5
61	74688.5
62	60040.0
63	46536.0
64	35220.5
65	26378.5
66	19705.0
67	15017.5
68	11396.5
69	8197.5
70	5802.5
71	4199.5
72	2979.0
73	2011.0
74	1358.5
75	896.0
76	565.0
77	335.5
78	200.5
79	125.0
80	87.5
81	63.5
82	49.0
83	34.5
84	19.5
85	11.5
86	7.5
87	5.5
88	4.5
89	3.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	5.869250108176351E-4
4	0.018822077933117264
5	7.083577716764562E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.007804326208283801
26-27	0.05213785455310599
28-29	0.08558799652663486
30-31	0.07589217165606393
32-33	0.03591402462986655
34-35	0.0045344745399953906
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	3.0728851032895457E-4
56-57	7.635129340750844E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.362459665430825E-5
72-73	0.012728826883663766
74-75	0.026476207904843403
76-77	0.003627683226818164
78-79	0.0
80-81	0.0
82-83	0.011528100275238077
84-85	0.04993372650519788
86-87	0.04062721075279584
88-89	0.011036157387709352
90-91	1.4332466045410724E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	55.0
20-21	221.0
22-23	617.0
24-25	1772.0
26-27	2228.0
28-29	3959.0
30-31	9342.0
32-33	12193.0
34-35	17519.0
36-37	28738.0
38-39	34448.0
40-41	34651.0
42-43	37472.0
44-45	44504.0
46-47	43742.0
48-49	38218.0
50-51	33958.0
52-53	32640.0
54-55	36212.0
56-57	39062.0
58-59	39020.0
60-61	39182.0
62-63	39070.0
64-65	41943.0
66-67	45773.0
68-69	41756.0
70-71	38280.0
72-73	36871.0
74-75	37321.0
76-77	37655.0
78-79	39538.0
80-81	39881.0
82-83	39258.0
84-85	39079.0
86-87	41310.0
88-89	44303.0
90-91	46277.0
92-93	46854.0
94-95	53239.0
96-97	156325.0
98-99	301888.0
100-101	3244632.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.76057183201983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.08939029715614	81.73906942437637
2	5.849091297968806	10.383373766108047
3	1.2201881003095008	3.249137805782918
4	0.3899729478675456	1.3845688740696722
5	0.17057783436858093	0.7570293060211404
6	0.09373359090841417	0.49919082773396745
7	0.05520404435306074	0.34299597809524995
8	0.02859360597558232	0.20303878537057166
9	0.023409922928581472	0.1870090331115963
>10	0.07779007638366454	1.0792657587948709
>50	0.0015883255034039303	0.0899905391427514
>100	4.2742723355162414E-4	0.07037370567489121
>500	3.2529043144401015E-5	0.01495619571794358
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.0238793476470176E-5	0.0	0.0	0.0
3	0.0	2.0238793476470176E-5	0.0	4.047758695294035E-5	0.0
4	0.0	4.047758695294035E-5	0.0	6.071638042941053E-5	0.0
5	0.0	4.047758695294035E-5	0.0	8.09551739058807E-5	0.0
6	0.0	4.047758695294035E-5	0.0	8.09551739058807E-5	0.0
7	0.0	4.047758695294035E-5	0.0	8.09551739058807E-5	0.0
8	0.0	6.071638042941053E-5	0.0	8.09551739058807E-5	0.0
9	0.0	1.4167155433529124E-4	0.0	1.2143276085882106E-4	0.0
10-11	0.0	1.4167155433529124E-4	0.0	1.2143276085882106E-4	0.0
12-13	0.0	1.4167155433529124E-4	0.0	1.2143276085882106E-4	0.0
14-15	0.0	2.631043151941123E-4	0.0	1.4167155433529124E-4	2.0238793476470176E-5
16-17	0.0	2.631043151941123E-4	0.0	1.4167155433529124E-4	2.0238793476470176E-5
18-19	0.0	2.631043151941123E-4	0.0	1.517909510735263E-4	2.0238793476470176E-5
20-21	0.0	2.833431086705825E-4	0.0	1.922685380264667E-4	2.0238793476470176E-5
22-23	0.0	3.035819021470526E-4	0.0	2.2262672824117195E-4	2.0238793476470176E-5
24-25	0.0	3.44059489099993E-4	0.0	2.428655217176421E-4	2.0238793476470176E-5
26-27	0.0	3.44059489099993E-4	0.0	2.732237119323474E-4	2.0238793476470176E-5
28-29	0.0	3.44059489099993E-4	0.0	5.768056140794001E-4	4.047758695294035E-5
30-31	0.0	3.642982825764632E-4	0.0	0.0013357603694470316	4.047758695294035E-5
32-33	0.0	3.642982825764632E-4	0.0	0.003329281526879344	6.071638042941053E-5
34-35	0.0	4.6549224995881403E-4	0.0	0.005990682869035173	6.071638042941053E-5
36-37	0.0	5.464474238646948E-4	0.0	0.010129516134973322	6.071638042941053E-5
38-39	0.0	5.869250108176351E-4	0.0	0.016160676590961435	8.09551739058807E-5
40-41	0.0	6.678801847235158E-4	0.0	0.021787061177420144	8.09551739058807E-5
42-43	0.0	6.88118978199986E-4	0.0	0.027474162144308263	8.09551739058807E-5
44-45	0.0	7.488353586293966E-4	0.0	0.03408212821437578	8.09551739058807E-5
46-47	0.0	7.893129455823368E-4	0.0	0.04138833265938151	1.0119396738235088E-4
48-49	0.0	7.893129455823368E-4	0.0	0.049038596593487235	1.0119396738235088E-4
50-51	0.0	8.297905325352772E-4	0.0	0.05685077087540473	1.0119396738235088E-4
52-53	0.0	8.297905325352772E-4	0.0	0.06372184126066635	1.0119396738235088E-4
54-55	0.0	8.399099292735123E-4	0.0	0.07085601596112209	1.0119396738235088E-4
56-57	0.0	8.500293260117474E-4	0.0	0.07618893804217197	1.0119396738235088E-4
58-59	0.0	8.702681194882176E-4	0.0	0.0818153226286307	1.0119396738235088E-4
60-61	0.0	9.309844999176281E-4	0.0	0.08701669255208352	1.0119396738235088E-4
62-63	0.0	9.815814836088036E-4	0.0	0.09223830126901283	1.0119396738235088E-4
64-65	0.0	0.0011738500216352701	0.0	0.0974801487794186	1.0119396738235088E-4
66-67	0.0	0.0011738500216352701	0.0	0.10266127990939497	1.0119396738235088E-4
68-69	0.0	0.0011738500216352701	0.0	0.10831802268606838	1.0119396738235088E-4
70-71	0.0	0.001183969418373505	0.0	0.11334736286497123	1.0119396738235088E-4
72-73	0.0	0.0011940888151117403	0.0	0.11718261422876233	1.4167155433529124E-4
74-75	0.0	0.0012042082118499755	0.0	0.11963150823941521	1.4167155433529124E-4
76-77	0.0	0.0012244470053264455	0.0	0.12230302897830927	1.4167155433529124E-4
78-79	0.0	0.0012345664020646807	0.0	0.12411440099445335	1.4167155433529124E-4
80-81	0.0	0.0012345664020646807	0.0	0.12454953505419747	1.517909510735263E-4
82-83	0.0	0.0012345664020646807	0.0	0.12494419152698863	1.821491412882316E-4
84-85	0.0	0.0012345664020646807	0.0	0.1250858630813239	1.821491412882316E-4
86-87	0.0	0.001275043989017621	0.0	0.12511622127153862	1.821491412882316E-4
88	0.0	0.0013155215759705614	0.0	0.12511622127153862	1.821491412882316E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6990	0.0	53.157303	1
GTATCAA	13410	0.0	39.959763	1
TCAACGC	16590	0.0	31.735785	4
CTTATAC	3930	0.0	31.623957	1
ATCAACG	16850	0.0	31.27135	3
TATCAAC	17105	0.0	31.004225	2
CAACGCA	17080	0.0	30.967638	5
AACGCAG	17505	0.0	30.264399	6
ACGCAGA	19830	0.0	26.630167	7
CGCAGAG	20085	0.0	26.292068	8
TATACAC	5970	0.0	24.38247	3
TGGTATC	3070	0.0	23.291466	2
TTATACA	5865	0.0	22.93216	2
GTGGTAT	3370	0.0	22.733616	1
GCAGAGT	23225	0.0	22.645796	9
CTCGTAT	5840	0.0	22.587788	9
TCTCGTA	5890	0.0	21.673586	8
GAGTACT	14105	0.0	21.148022	12-13
GTACATG	15970	0.0	21.081305	1
AATCTCG	6080	0.0	21.066273	6
>>END_MODULE