FastQCFastQC Report
Fri 27 May 2016
SRR2138769_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138769_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5913750
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82900.049.774671
GTATCAA151150.038.2306331
TCAACGC180150.031.5533874
ATCAACG181250.031.4330063
CAACGCA187250.030.375215
TATCAAC191000.029.9853152
AACGCAG191300.029.7992526
ACGCAGA220900.025.7481127
CGCAGAG223900.025.3648878
GTGGTAT38550.024.9829671
TGGTATC36900.024.5920852
GCAGAGT261750.021.6806819
GAGTACT158500.020.54809212-13
GTACATG179200.019.9927861
TACATGG176950.019.6906342
CAGAGTA253800.019.17277510-11
ACATGGG181750.018.817343
GTACTTT173200.018.7304614-15
AGAGTAC236900.018.69783810-11
AGTACTT169900.017.31790712-13
Produced by FastQC (version 0.11.5)